FastQCFastQC Report
Thu 26 May 2016
SRR1660720_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660720_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27848634
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15563535.58861522615436No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT333780.11985507080885906No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT287030.10306789194759068No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2007300.085.550468
GTGGTAT2008150.084.706846
TGGTATC2033650.084.418197
AGTGGTA2017500.084.407365
GTATCAA2035250.084.331489
GCAGTGG2046800.083.301383
AAGCAGT2021100.082.208431
AGCAGTG2128350.079.82792
CAGTGGT2149200.079.161434
CAACGCA1976200.044.32951712-13
ATCAACG1982250.044.23921210-11
ACGCAGA2000900.043.82225414-15
TCAACGC1969900.043.51936312-13
GAGTACT1968050.043.32267820-21
AGAGTAC2025550.043.228918-19
AACGCAG1985400.043.22460614-15
CGCAGAG2008400.042.74132516-17
TATCAAC2024950.042.32118610-11
AGTACTT2088700.041.7068120-21
GTACTTT2070650.041.1915422-23
Produced by FastQC (version 0.11.5)