##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660720_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 27848634 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.217825657086088 34.0 31.0 34.0 27.0 34.0 2 31.873358204930266 34.0 31.0 34.0 28.0 34.0 3 31.63876773991859 34.0 31.0 34.0 30.0 34.0 4 35.56474798728009 37.0 35.0 37.0 33.0 37.0 5 35.905417982081275 37.0 35.0 37.0 35.0 37.0 6 35.36671166707853 37.0 35.0 37.0 33.0 37.0 7 35.58024027318539 37.0 35.0 37.0 35.0 37.0 8 35.954602405274166 37.0 35.0 37.0 35.0 37.0 9 37.76969092990342 39.0 38.0 39.0 35.0 39.0 10-11 37.78240440805822 39.0 37.5 39.0 35.0 39.0 12-13 37.86132808165743 39.0 38.0 39.0 35.0 39.0 14-15 39.3195588875203 41.0 39.0 41.0 36.0 41.0 16-17 39.28619337307532 41.0 39.0 41.0 36.0 41.0 18-19 39.28146490057645 41.0 39.0 41.0 36.0 41.0 20-21 39.21828244789314 40.5 39.0 41.0 36.0 41.0 22-23 39.09460984693181 40.0 39.0 41.0 35.5 41.0 24-25 39.106008664554246 40.0 39.0 41.0 36.0 41.0 26-27 39.064455585146476 40.0 39.0 41.0 35.5 41.0 28-29 38.99012962359303 40.0 39.0 41.0 35.0 41.0 30-31 38.89948797847679 40.0 38.5 41.0 35.0 41.0 32-33 38.78113150899969 40.0 38.0 41.0 35.0 41.0 34-35 38.6990486319724 40.0 38.0 41.0 35.0 41.0 36-37 38.57411695668807 40.0 38.0 41.0 34.5 41.0 38-39 38.17024639341376 40.0 37.0 41.0 34.0 41.0 40-41 38.0444223045195 40.0 37.0 41.0 33.5 41.0 42-43 37.88578477493726 40.0 37.0 41.0 33.0 41.0 44-45 37.768696428700956 40.0 36.0 41.0 33.0 41.0 46-47 37.62192134809916 40.0 36.0 41.0 33.0 41.0 48-49 37.67101018312066 40.0 36.0 41.0 33.0 41.0 50-51 37.67092046597331 40.0 36.0 41.0 33.0 41.0 52-53 37.419829784110775 40.0 36.0 41.0 32.5 41.0 54-55 36.883213176631926 40.0 36.0 41.0 30.5 41.0 56-57 36.36716759608389 39.5 35.0 41.0 28.5 41.0 58-59 36.21796483805992 39.0 35.0 41.0 28.0 41.0 60-61 36.07194706569809 39.0 35.0 41.0 28.0 41.0 62-63 35.84112870670784 39.0 35.0 41.0 28.0 41.0 64-65 35.583047915384284 38.0 35.0 40.5 28.0 41.0 66-67 35.315644673990114 37.5 35.0 40.0 28.0 41.0 68-69 35.063537622707095 37.0 35.0 40.0 28.0 41.0 70-71 34.77845356795597 36.5 34.0 39.0 27.5 41.0 72-73 34.442731517818785 36.0 34.0 39.0 26.5 41.0 74-75 34.06408145548539 35.5 34.0 39.0 26.0 40.5 76-77 32.361394099258156 34.5 32.0 36.5 24.0 39.0 78-79 33.29297945098492 35.0 33.5 37.0 26.0 39.0 80-81 33.187752512385345 35.0 34.0 37.0 26.0 39.0 82-83 32.921191502606554 35.0 34.0 36.0 25.5 38.0 84-85 32.6691057809155 35.0 34.0 36.0 25.0 37.0 86-87 32.45453462816165 35.0 34.0 35.5 25.0 37.0 88-89 32.28550089745874 35.0 33.0 35.0 25.0 36.5 90-91 32.12751079640029 35.0 33.0 35.0 24.5 36.0 92-93 31.989360537396557 35.0 33.0 35.0 24.0 36.0 94-95 31.886482403409804 35.0 33.0 35.0 24.0 36.0 96-97 31.78511604913907 35.0 33.0 35.0 23.0 35.5 98-99 31.69787334991009 35.0 33.0 35.0 23.0 35.0 100 31.647083120845352 35.0 33.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.5817506319674628 1101 2 1.2012929658560658 1101 3 1.5638579650187516 1101 4 0.9717472922242223 1101 5 0.5946246459642737 1101 6 1.1291591529049612 1101 7 0.8423352597052798 1101 8 0.5009931020392315 1101 9 0.5198775581487709 1101 10-11 0.44542324861219385 1101 12-13 0.40192961833437124 1101 14-15 0.5204669083561626 1101 16-17 0.5431320310421768 1101 18-19 0.5149679729300232 1101 20-21 0.5262015130491307 1101 22-23 0.5163162323878723 1101 24-25 0.5214605776105046 1101 26-27 0.535057688122663 1101 28-29 0.5018992831949802 1101 30-31 0.4895447269047324 1101 32-33 0.5483595815606037 1101 34-35 0.5541073533097389 1101 36-37 0.568395309904453 1101 38-39 0.5821340244132713 1101 40-41 0.5348552479806656 1101 42-43 0.5606035683402553 1101 44-45 0.5306140044073473 1101 46-47 0.5319991186293649 1101 48-49 0.5249260506203157 1101 50-51 0.4983531770704488 1101 52-53 0.4957581671389093 1101 54-55 0.5233449214691746 1101 56-57 0.5283396503282631 1101 58-59 0.485237072294062 1101 60-61 0.48816055818394233 1101 62-63 0.4431685875728135 1101 64-65 0.4476503634619533 1101 66-67 0.46899110622649687 1101 68-69 0.4306794765610107 1101 70-71 0.45753346546680973 1101 72-73 0.40398998567139444 1101 74-75 0.4218917858498159 1101 76-77 0.38874718960681065 1101 78-79 0.46165309547200906 1101 80-81 0.4668230529394535 1101 82-83 0.4526529673159345 1101 84-85 0.44669272194804677 1101 86-87 0.4330055623375557 1101 88-89 0.4544213591302011 1101 90-91 0.45318106783609124 1101 92-93 0.43180370550949476 1101 94-95 0.42749527154557754 1101 96-97 0.42499534210178425 1101 98-99 0.4201741356200692 1101 100 0.4318955138975298 1102 1 1.4695164561231628 1102 2 1.1047277284633985 1102 3 1.488797191854534 1102 4 0.899770824540937 1102 5 0.489762596448891 1102 6 1.0395336167753157 1102 7 0.7599856324691601 1102 8 0.41517295452714365 1102 9 0.4340071844206079 1102 10-11 0.32949846908739744 1102 12-13 0.329149880907714 1102 14-15 0.42661790449849946 1102 16-17 0.4533218248429449 1102 18-19 0.38654258015694154 1102 20-21 0.37500512108497475 1102 22-23 0.39812613023167387 1102 24-25 0.39669764423592113 1102 26-27 0.43039753592886854 1102 28-29 0.4385485594866836 1102 30-31 0.4290372952809278 1102 32-33 0.4324087224108055 1102 34-35 0.4059611889374608 1102 36-37 0.3899512783418757 1102 38-39 0.4115655909277294 1102 40-41 0.4414047949793627 1102 42-43 0.4482068696916315 1102 44-45 0.41766141631820375 1102 46-47 0.40604501075282684 1102 48-49 0.38810308296931595 1102 50-51 0.36915714939288335 1102 52-53 0.36325834143861613 1102 54-55 0.37749222016726947 1102 56-57 0.36141326697714504 1102 58-59 0.4108651105919918 1102 60-61 0.38243577219213165 1102 62-63 0.3394353933523391 1102 64-65 0.306896310990858 1102 66-67 0.307828703339446 1102 68-69 0.2931156237048427 1102 70-71 0.2711849959604393 1102 72-73 0.26240295026619265 1102 74-75 0.2741901818075121 1102 76-77 0.09241857900710215 1102 78-79 0.3156794460061363 1102 80-81 0.35155906549262994 1102 82-83 0.3327776323620881 1102 84-85 0.30857855393702494 1102 86-87 0.31396773161880276 1102 88-89 0.34968065841255935 1102 90-91 0.36508360461990463 1102 92-93 0.3442459296046678 1102 94-95 0.2786360319082739 1102 96-97 0.31086484558038663 1102 98-99 0.2826411248387366 1102 100 0.26729337996797753 1103 1 1.4914505888723575 1103 2 1.1128897746557662 1103 3 1.4536841346827778 1103 4 0.9075434379210066 1103 5 0.5278409304166445 1103 6 1.0423228690107535 1103 7 0.7652274974838846 1103 8 0.4328489896055885 1103 9 0.441230982795048 1103 10-11 0.3594093578042816 1103 12-13 0.33091755465297723 1103 14-15 0.39967612370980277 1103 16-17 0.44186212896715915 1103 18-19 0.4147175438628139 1103 20-21 0.4191432171248053 1103 22-23 0.39181345756512087 1103 24-25 0.38457192820175834 1103 26-27 0.40567745795625143 1103 28-29 0.39567519733382284 1103 30-31 0.3781905950269149 1103 32-33 0.4030688009798098 1103 34-35 0.4227370533950179 1103 36-37 0.3783383957101236 1103 38-39 0.43070414563921844 1103 40-41 0.4626572646672784 1103 42-43 0.4422293554648462 1103 44-45 0.42986260476946114 1103 46-47 0.4632355382274369 1103 48-49 0.39392442623648805 1103 50-51 0.40868869298774513 1103 52-53 0.39507946015177 1103 54-55 0.4474341735839502 1103 56-57 0.5185319653349438 1103 58-59 0.49548639725204424 1103 60-61 0.4647558999510224 1103 62-63 0.4361726133569661 1103 64-65 0.43460579852223447 1103 66-67 0.4730232270183663 1103 68-69 0.4406795653186464 1103 70-71 0.42742839146989553 1103 72-73 0.4136898770505084 1103 74-75 0.43492322702032027 1103 76-77 0.2745347975936525 1103 78-79 0.40759864305106674 1103 80-81 0.46477558485073445 1103 82-83 0.46643901963165035 1103 84-85 0.4368975985426147 1103 86-87 0.4039693290590307 1103 88-89 0.38986932099135174 1103 90-91 0.38289002589683463 1103 92-93 0.3983281087804613 1103 94-95 0.40185738711296537 1103 96-97 0.42234778826960806 1103 98-99 0.4043787206845302 1103 100 0.418325290808788 1104 1 1.4132794643923 1104 2 1.0547227342512322 1104 3 1.3944404908453656 1104 4 0.8809122965785647 1104 5 0.4646718444768041 1104 6 1.010142692649275 1104 7 0.7372249724419007 1104 8 0.41444669488941344 1104 9 0.41517162060426216 1104 10-11 0.31910790739126327 1104 12-13 0.2880938338925674 1104 14-15 0.35610072795245884 1104 16-17 0.3789555287545312 1104 18-19 0.32850208469989184 1104 20-21 0.33047943427335014 1104 22-23 0.32764298353585986 1104 24-25 0.3546036382554121 1104 26-27 0.34623143995430183 1104 28-29 0.3375221091601972 1104 30-31 0.33256065449031524 1104 32-33 0.3532992564631101 1104 34-35 0.3821307062343138 1104 36-37 0.36291990361695525 1104 38-39 0.39211780996023293 1104 40-41 0.3692746779515872 1104 42-43 0.3663764410874819 1104 44-45 0.36232291361737623 1104 46-47 0.39297742225249266 1104 48-49 0.33003660490507514 1104 50-51 0.306400453537492 1104 52-53 0.33588218351901844 1104 54-55 0.315401272530778 1104 56-57 0.3351241569716379 1104 58-59 0.33059362990509555 1104 60-61 0.3413714369679539 1104 62-63 0.2983368545780607 1104 64-65 0.29567378902205377 1104 66-67 0.3288292071270078 1104 68-69 0.25723836200935324 1104 70-71 0.2584687938046599 1104 72-73 0.26569858024659254 1104 74-75 0.2850536022620531 1104 76-77 0.17086320525439191 1104 78-79 0.2985193742125034 1104 80-81 0.3329731620478995 1104 82-83 0.31237800414522354 1104 84-85 0.2872859720966261 1104 86-87 0.29633177919972553 1104 88-89 0.2868707039598348 1104 90-91 0.28902064044975617 1104 92-93 0.29032302643989283 1104 94-95 0.29042051130825186 1104 96-97 0.2860514965755989 1104 98-99 0.3304419918756558 1104 100 0.3320084721610783 1105 1 1.3700504035607075 1105 2 1.0118230930434216 1105 3 1.3437642512305636 1105 4 0.8131206759940213 1105 5 0.4276961206145913 1105 6 0.9831429970724628 1105 7 0.6970498665192295 1105 8 0.3449595564712169 1105 9 0.34704325833068594 1105 10-11 0.24896580611297736 1105 12-13 0.2453394839030878 1105 14-15 0.30545325577589466 1105 16-17 0.3141893736164434 1105 18-19 0.2731935162224204 1105 20-21 0.29782089721905436 1105 22-23 0.2947752162525106 1105 24-25 0.2774579800418522 1105 26-27 0.30919397186813313 1105 28-29 0.29482222562227633 1105 30-31 0.27439121277313205 1105 32-33 0.34251029870934246 1105 34-35 0.33654272157659904 1105 36-37 0.3401568597670419 1105 38-39 0.3648580497159841 1105 40-41 0.365265130907126 1105 42-43 0.3426227297519375 1105 44-45 0.3248109301885478 1105 46-47 0.29746589223575626 1105 48-49 0.29046515392888494 1105 50-51 0.31583420759357494 1105 52-53 0.27963612951499783 1105 54-55 0.30927714389549266 1105 56-57 0.3021331150678961 1105 58-59 0.28737510313357717 1105 60-61 0.23758882782046697 1105 62-63 0.2522702781087318 1105 64-65 0.2509870751455736 1105 66-67 0.22218314506351788 1105 68-69 0.17782604654487244 1105 70-71 0.1958620400330915 1105 72-73 0.17731458436166037 1105 74-75 0.2005081372251567 1105 76-77 0.12829743128280313 1105 78-79 0.20569454117376296 1105 80-81 0.2671616683091358 1105 82-83 0.2658153493551012 1105 84-85 0.24077809075328105 1105 86-87 0.25006974146634064 1105 88-89 0.2597097595222877 1105 90-91 0.2639701308764728 1105 92-93 0.23478514169247688 1105 94-95 0.22680855436165004 1105 96-97 0.25144123507963556 1105 98-99 0.30475978234921186 1105 100 0.28641763443329893 1106 1 1.3228361692622137 1106 2 0.9756396222838113 1106 3 1.3319652085394829 1106 4 0.7832000972932462 1106 5 0.39221213290576884 1106 6 0.9445916566216468 1106 7 0.6791228293252232 1106 8 0.31866344875905384 1106 9 0.29858814768004294 1106 10-11 0.20659793556723116 1106 12-13 0.17868652119650363 1106 14-15 0.24445626148930444 1106 16-17 0.24055322777104493 1106 18-19 0.21062233114989226 1106 20-21 0.2152240940586907 1106 22-23 0.2338357844376162 1106 24-25 0.21838016041966313 1106 26-27 0.17919317401337764 1106 28-29 0.20953399575021336 1106 30-31 0.2359873586646799 1106 32-33 0.2764247607040744 1106 34-35 0.28417406072453133 1106 36-37 0.26287405268671193 1106 38-39 0.26222443471656476 1106 40-41 0.2966136089820921 1106 42-43 0.3166026574240277 1106 44-45 0.3176695689979212 1106 46-47 0.30137575082789425 1106 48-49 0.27266156061724445 1106 50-51 0.2688952965633433 1106 52-53 0.27332821890760073 1106 54-55 0.30074814636865455 1106 56-57 0.2837843606492001 1106 58-59 0.2748793133413301 1106 60-61 0.2673798498352369 1106 62-63 0.24238012570294387 1106 64-65 0.24455204827366828 1106 66-67 0.23906422202102107 1106 68-69 0.2195728899946161 1106 70-71 0.22102686390736892 1106 72-73 0.1494583615482057 1106 74-75 0.1839320623939571 1106 76-77 0.060857746332033 1106 78-79 0.23507707921525167 1106 80-81 0.2586445730889224 1106 82-83 0.2502724294906997 1106 84-85 0.24532798922619747 1106 86-87 0.2759131371054053 1106 88-89 0.2585962607812178 1106 90-91 0.236477621304104 1106 92-93 0.23648319935766438 1106 94-95 0.2190131865593763 1106 96-97 0.24621177144012663 1106 98-99 0.2334320701461614 1106 100 0.21874743397649254 1107 1 1.2556374002364343 1107 2 0.8992098568891898 1107 3 1.2707858222517068 1107 4 0.7715931078627847 1107 5 0.37752904866560044 1107 6 0.9380776635061423 1107 7 0.6567482746382822 1107 8 0.30148125045342766 1107 9 0.28878230355326195 1107 10-11 0.1944765314451331 1107 12-13 0.12979728871869867 1107 14-15 0.17453899545735396 1107 16-17 0.1826385876704535 1107 18-19 0.15791298644350604 1107 20-21 0.14954272287812387 1107 22-23 0.18478219940332963 1107 24-25 0.1740361806605648 1107 26-27 0.1459099305292213 1107 28-29 0.1367654715375366 1107 30-31 0.1273386317862446 1107 32-33 0.16858614599838972 1107 34-35 0.18130438732043785 1107 36-37 0.18646206659407483 1107 38-39 0.18974808855761438 1107 40-41 0.20354422069203082 1107 42-43 0.21174128686121918 1107 44-45 0.1923588215430172 1107 46-47 0.22760756217635958 1107 48-49 0.2031791237158984 1107 50-51 0.1998577827197181 1107 52-53 0.21999423578626676 1107 54-55 0.27709225381546787 1107 56-57 0.2155084096928661 1107 58-59 0.18796501871165816 1107 60-61 0.18192371233705984 1107 62-63 0.17325279922514625 1107 64-65 0.12917668411841277 1107 66-67 0.1331658100776849 1107 68-69 0.15243556819726223 1107 70-71 0.17814297090371412 1107 72-73 0.13282020458402855 1107 74-75 0.1616095936427584 1107 76-77 -0.021051967577072617 1107 78-79 0.12108329400867746 1107 80-81 0.16451961624629519 1107 82-83 0.19772770826978814 1107 84-85 0.17697346819578286 1107 86-87 0.15690057820791026 1107 88-89 0.19676246700878153 1107 90-91 0.1730295265288717 1107 92-93 0.1748116735119396 1107 94-95 0.11524238738432402 1107 96-97 0.17000711029139737 1107 98-99 0.23151362053203073 1107 100 0.21743990409830616 1108 1 1.2794078537045728 1108 2 0.9117956778776595 1108 3 1.2663703820833554 1108 4 0.7594625445822913 1108 5 0.356830171550655 1108 6 0.8811397244557995 1108 7 0.6231287811898625 1108 8 0.2699435543509523 1108 9 0.23278912161278953 1108 10-11 0.14405104289181025 1108 12-13 0.13883925466140568 1108 14-15 0.1680529419110215 1108 16-17 0.1624639139770352 1108 18-19 0.13220372681767856 1108 20-21 0.15284624620774423 1108 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-0.7612275349424635 2316 92-93 -0.8783170192318579 2316 94-95 -0.9184440020120057 2316 96-97 -0.9591493179587154 2316 98-99 -1.1445684219236618 2316 100 -1.0962691732942993 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 48.0 7 831.0 8 995.0 9 403.0 10 372.0 11 701.0 12 909.0 13 1417.0 14 2873.0 15 7022.0 16 14070.0 17 21299.0 18 28082.0 19 37736.0 20 53083.0 21 78418.0 22 119126.0 23 201772.0 24 351505.0 25 491656.0 26 502473.0 27 414877.0 28 349405.0 29 356345.0 30 405587.0 31 492187.0 32 610473.0 33 777348.0 34 1067666.0 35 1644205.0 36 2977929.0 37 6133859.0 38 8189247.0 39 2509722.0 40 4993.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.50938777274799 33.591344118659 28.528392090601056 21.370876017991954 2 20.666965229239064 34.04788686539788 27.128632815043215 18.156515090319846 3 25.718865532042663 25.997492230895393 27.115834030866193 21.167808206195755 4 19.538646186630267 27.822120260605388 25.577297328664052 27.061936224100297 5 21.03508672583201 32.88385210284904 26.474522636182048 19.60653853513691 6 28.26244358646035 27.00596118271566 25.825298949743576 18.90629628108042 7 20.43290485728841 26.24613945293262 32.922608209695156 20.39834748008381 8 26.798077270768133 26.769702353146783 26.16061115450258 20.271609221582505 9 26.297555564125695 25.717440934445833 27.467038419191404 20.51796508223707 10-11 20.073199691855407 30.062856448388125 29.722394268483264 20.141549591273204 12-13 20.02290130280717 26.799761525107478 29.668550349722718 23.508786822362634 14-15 19.994908906483527 33.37304443729628 26.43666651656954 20.195380139650656 16-17 23.411238970798077 26.6052744109301 26.480282609269796 23.503204009002022 18-19 20.05903772515377 29.93199953721249 26.50426049622398 23.50470224140976 20-21 23.36496054434163 30.00856903075405 26.46396675617525 20.162503668729066 22-23 23.367307124402267 26.709781064945975 29.752216842024225 20.170694968627533 24-25 20.161860516357013 29.863203684624267 26.329169300562285 23.64576649845643 26-27 20.21452045366493 26.586649241347576 32.983428267303886 20.215402037683607 28-29 20.17334626785682 26.70275910565627 32.946422229358056 20.177472397128845 30-31 20.181580366633632 26.61579200262893 32.94510029755921 20.257527333178235 32-33 20.334927847327457 26.708077378279466 32.74846970166327 20.20852507272981 34-35 20.305498637179063 26.81691262611633 32.67337783584654 20.204210900858072 36-37 20.250360264730812 26.769265259609927 32.711060800103624 20.269313675555637 38-39 20.276424940161238 26.76125045653276 32.710532367155814 20.25179223615019 40-41 20.276644254117173 26.795557859312318 32.71363230107665 20.21416558549386 42-43 20.30569506447188 26.824122727972423 32.6430877343499 20.2270944732058 44-45 20.29919633651654 26.8947830616873 32.6072873643173 20.198733237478862 46-47 20.339654487975732 26.957001994604795 32.53352237989613 20.169821137523346 48-49 20.369046448776785 26.97112235929905 32.44733452174347 20.212496670180695 50-51 20.37560443644202 27.023779791196358 32.3783464312711 20.222269341090524 52-53 20.48011114432124 27.153373166444144 32.1236095394588 20.24290614977582 54-55 20.80064212695736 27.379444088762906 31.371061423137526 20.448852361142208 56-57 21.27316714971126 27.94865187189582 30.136205465781714 20.641975512611207 58-59 21.352848867857702 28.521333799838228 29.411791536096654 20.71402579620742 60-61 21.355486359994842 28.85832466632987 29.04585589317657 20.74033308049872 62-63 21.327126370181865 29.024085490053032 28.86030981825778 20.788478321507327 64-65 21.312339161993336 29.120932721634812 28.793104163671824 20.77362395270003 66-67 21.287494452399887 28.96970384783358 28.947345494790067 20.795456204976468 68-69 21.215454875407847 28.79967112776429 29.18021122514518 20.80466277168268 70-71 21.154798448581282 28.690368322175857 29.321919383744167 20.832913845498695 72-73 21.112155450611354 28.624583318241374 29.34922545199694 20.914035779150332 74-75 21.181770476559528 28.69234692511844 29.169576548624 20.956306049698036 76-77 21.23006243938397 28.840498544963765 28.95345145200554 20.97598756364672 78-79 21.33980611650345 29.05065816898308 28.55977610407327 21.0497596104402 80-81 21.426290442046977 29.232877709921247 28.219620049619294 21.121211798412485 82-83 21.458002127096684 29.43520567754611 27.839741256406835 21.267050938950373 84-85 21.473608102882974 29.54222249965533 27.605023076097456 21.37914632136424 86-87 21.479966722135764 29.621096915581763 27.39817546618023 21.500760896102246 88-89 21.501843086882715 29.707925668845714 27.176271889486365 21.613959354785205 90-91 21.553825548381894 29.715094597337675 26.9785975702901 21.75248228399033 92-93 21.538464081336077 29.755994217260074 26.88168473000358 21.823856971400275 94-95 21.560243377123143 29.843036647002126 26.722511442395895 21.874208533478836 96-97 21.56497214425873 29.87838772870465 26.588783426305586 21.96785670073104 98-99 21.546610554626717 29.896767273108477 26.496075373747157 22.06054679851765 100 21.521476566247372 29.90757904544708 26.398512393269314 22.172431995036234 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 29168.0 1 20855.5 2 10120.5 3 7177.0 4 6492.0 5 6460.5 6 6659.0 7 6689.5 8 6760.5 9 7047.5 10 7696.5 11 14888.5 12 116934.5 13 138828.5 14 64797.0 15 69269.5 16 80157.0 17 90713.0 18 100326.0 19 110158.0 20 121574.0 21 139367.5 22 164480.5 23 193531.5 24 226934.0 25 264776.0 26 305380.5 27 353122.5 28 412556.5 29 472342.5 30 519635.5 31 562110.0 32 607283.0 33 644870.0 34 673558.0 35 710463.0 36 761088.5 37 803747.0 38 834237.5 39 852851.0 40 857031.5 41 866556.5 42 891104.5 43 897916.0 44 900845.5 45 915190.5 46 915540.5 47 918116.0 48 908268.0 49 881802.5 50 868537.5 51 856876.5 52 833693.5 53 797416.5 54 745608.5 55 689942.5 56 633510.0 57 573700.0 58 508365.5 59 435038.0 60 359250.0 61 292442.5 62 230044.5 63 167912.0 64 117179.5 65 80719.5 66 55901.5 67 40109.0 68 28346.0 69 19464.0 70 13654.5 71 9878.0 72 7498.5 73 5680.5 74 4490.5 75 3868.0 76 2793.5 77 1501.0 78 748.5 79 444.0 80 295.0 81 224.5 82 174.5 83 120.0 84 90.0 85 68.0 86 48.0 87 30.5 88 17.0 89 15.5 90 14.5 91 7.5 92 5.0 93 4.0 94 2.0 95 2.5 96 3.5 97 2.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3295816951021727 2 0.006732825746498015 3 2.4341265715223233 4 8.833467379405395E-4 5 3.949924437945502E-5 6 0.008912465868164306 7 0.007871122152705946 8 3.411298378225661E-4 9 0.0 10-11 7.002138776357936E-5 12-13 0.0 14-15 0.0 16-17 2.693130298599206E-5 18-19 0.0 20-21 3.3574357722536766E-4 22-23 3.590840398132275E-5 24-25 5.386260597198412E-4 26-27 0.0037093381312706397 28-29 0.006147518761602454 30-31 0.005639414845266737 32-33 0.015471135855352905 34-35 0.009752722521327258 36-37 0.005039744498778647 38-39 0.0054167827405825365 40-41 0.0083666581276482 42-43 0.019146361002841287 44-45 0.03431586626475108 46-47 0.033339157676459105 48-49 0.04008275594415152 50-51 0.0403484781336133 52-53 0.06259732524044087 54-55 0.033321203474468444 56-57 0.022044169204134035 58-59 0.01686258650962916 60-61 0.017970360772452967 62-63 0.024748072023927637 64-65 0.0452104760326844 66-67 0.04811546591477341 68-69 0.05069009848023426 70-71 0.06131719063850672 72-73 0.06909495094086122 74-75 0.060142985828317466 76-77 0.059898808681244475 78-79 0.05446048089827315 80-81 0.05206539035271892 82-83 0.04851225377876703 84-85 0.050618281672271614 86-87 0.058598924457120595 88-89 0.06517375322610078 90-91 0.060891676051328045 92-93 0.0654538387771551 94-95 0.07377740682002572 96-97 0.07339318689742555 98-99 0.04801671780382478 100 0.007278633487014121 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.7848634E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.453234865404205 #Duplication Level Percentage of deduplicated Percentage of total 1 70.21029265756826 26.99812873530237 2 14.878071215884614 11.44219933617242 3 5.291660914481229 6.104444399178788 4 2.5692457234630703 3.951832369250431 5 1.5450613882563426 2.970630422204431 6 0.9949102608268577 2.295451075774543 7 0.7018301117412626 1.8891346685679753 8 0.5395977357483783 1.6599422772458166 9 0.41365753126202276 1.431582318311566 >10 2.507118483679157 18.35980277231319 >50 0.2221240013965797 5.862701456049983 >100 0.12027023456057723 9.284389447142075 >500 0.005473386809264498 1.2464250856048666 >1k 6.01970211916075E-4 0.4157091523777994 >5k 3.750404913101357E-5 0.10733576869693966 >10k+ 4.688006141296713E-5 5.980290715806829 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1556353 5.58861522615436 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 33378 0.11985507080885906 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 28703 0.10306789194759068 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.811726133497248E-4 1.0772521194396824E-5 0.0 0.0 7.181680796264549E-6 2 4.883542941459893E-4 1.4363361592529098E-5 0.0 3.5908403981322746E-6 7.181680796264549E-6 3 4.919451345441216E-4 2.1545042388793648E-5 0.0 3.5908403981322746E-6 1.0772521194396824E-5 4 4.919451345441216E-4 2.5135882786925922E-5 0.0 1.4363361592529098E-5 1.0772521194396824E-5 5 4.919451345441216E-4 3.2317563583190474E-5 0.0 1.4363361592529098E-5 1.0772521194396824E-5 6 4.919451345441216E-4 3.590840398132275E-5 0.0 1.7954201990661374E-5 1.0772521194396824E-5 7 4.95535974942254E-4 3.949924437945502E-5 0.0 1.7954201990661374E-5 1.0772521194396824E-5 8 4.95535974942254E-4 3.949924437945502E-5 0.0 1.7954201990661374E-5 1.4363361592529098E-5 9 4.95535974942254E-4 3.949924437945502E-5 0.0 1.7954201990661374E-5 1.4363361592529098E-5 10-11 4.9733139514132E-4 5.0271765573851845E-5 0.0 2.3340462587859785E-5 1.4363361592529098E-5 12-13 5.188764375301136E-4 5.924886656918253E-5 0.0 2.8726723185058197E-5 1.7954201990661374E-5 14-15 5.314443789235766E-4 6.104428676824867E-5 0.0 3.2317563583190474E-5 2.8726723185058197E-5 16-17 5.601711021086348E-4 6.643054736544708E-5 0.0 4.3090084777587297E-5 3.949924437945502E-5 18-19 5.906932454927591E-4 7.540764836077776E-5 0.0 4.668092517571957E-5 5.565802617105026E-5 20-21 6.122382878815528E-4 8.977100995330686E-5 0.0 5.924886656918253E-5 6.104428676824867E-5 22-23 6.589192130572723E-4 1.0054353114770369E-4 0.0 6.104428676824867E-5 6.104428676824867E-5 24-25 8.258932915704231E-4 1.0413437154583597E-4 0.0 6.283970696731481E-5 6.283970696731481E-5 26-27 0.0013752918724846612 1.1490689274023279E-4 0.0 6.822596756451322E-5 6.643054736544708E-5 28-29 0.0030629868596068303 1.2567941393462962E-4 0.0 6.822596756451322E-5 7.72030685598439E-5 30-31 0.007623354165234819 1.3106567453182802E-4 0.0 7.18168079626455E-5 8.079390895797617E-5 32-33 0.017324009500789158 1.3286109473089415E-4 0.0 7.361222816171164E-5 8.438474935610845E-5 34-35 0.035089692370548584 1.5081529672155553E-4 0.0 7.899848875891004E-5 8.618016955517459E-5 36-37 0.063985185054319 1.633832381150185E-4 0.0 8.258932915704231E-5 8.618016955517459E-5 38-39 0.10629426204531253 1.6697407851315075E-4 0.0 8.258932915704231E-5 8.797558975424073E-5 40-41 0.16314624264874178 1.7236033911034919E-4 0.0 8.258932915704231E-5 9.336185035143914E-5 42-43 0.23694698993135535 1.7954201990661372E-4 0.0 8.258932915704231E-5 1.0054353114770369E-4 44-45 0.331272980929693 1.8133744010567987E-4 0.0 8.258932915704231E-5 1.0413437154583596E-4 46-47 0.4507797402199333 1.9031454110101056E-4 0.0 8.258932915704231E-5 1.1311147254116665E-4 48-49 0.5970975093428281 1.974962218972751E-4 0.0 8.258932915704231E-5 1.1490689274023279E-4 50-51 0.7702945142659421 2.0108706229540738E-4 0.0 8.258932915704231E-5 1.1490689274023279E-4 52-53 0.9665752366884495 2.028824824944735E-4 0.0 8.797558975424073E-5 1.238839937355635E-4 54-55 1.1913762089731224 2.1365500368887032E-4 0.0 9.874811094863755E-5 1.2567941393462962E-4 56-57 1.4497623115015266 2.1904126428606876E-4 0.0 1.0413437154583597E-4 1.3286109473089415E-4 58-59 1.7452669312254239 2.2263210468420104E-4 0.0 1.0592979174490211E-4 1.3286109473089415E-4 60-61 2.0758001990331016 2.2801836528139945E-4 0.0 1.1131605234210052E-4 1.3286109473089415E-4 62-63 2.4374534851511926 2.4058630667486242E-4 0.0 1.1131605234210052E-4 1.3645193512902643E-4 64-65 2.827478001254927 2.5135882786925923E-4 0.0 1.1131605234210052E-4 1.3645193512902643E-4 66-67 3.252026652366504 2.5135882786925923E-4 0.0 1.1131605234210052E-4 1.3645193512902643E-4 68-69 3.7147477323304257 2.549496682673915E-4 0.0 1.1131605234210052E-4 1.3645193512902643E-4 70-71 4.211985765621394 2.585405086655238E-4 0.0 1.1490689274023279E-4 1.3824735532809256E-4 72-73 4.739550241494789 2.7110845005898676E-4 0.0 1.1849773313836507E-4 1.4183819572622487E-4 74-75 5.298829019764488 2.8367639145244973E-4 0.0 1.1849773313836507E-4 1.4722445632342325E-4 76-77 5.886136102761808 2.87267231850582E-4 0.0 1.1849773313836507E-4 1.5081529672155553E-4 78-79 6.506577665532895 2.890626520496481E-4 0.0 1.2208857353649733E-4 1.544061371196878E-4 80-81 7.158286471070717 2.9624433284591265E-4 0.0 1.3286109473089415E-4 1.579969775178201E-4 82-83 7.837637565993363 3.034260136421772E-4 0.0 1.3286109473089415E-4 1.579969775178201E-4 84-85 8.54396125856658 3.0522143384124336E-4 0.0 1.3286109473089415E-4 1.6517865831408465E-4 86-87 9.277507112198034 3.088122742393756E-4 0.0 1.3286109473089415E-4 1.741557593094153E-4 88 9.84296393137272 3.159939550356402E-4 0.0 1.3286109473089415E-4 1.7595117950848147E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 200730 0.0 85.55046 8 GTGGTAT 200815 0.0 84.70684 6 TGGTATC 203365 0.0 84.41819 7 AGTGGTA 201750 0.0 84.40736 5 GTATCAA 203525 0.0 84.33148 9 GCAGTGG 204680 0.0 83.30138 3 AAGCAGT 202110 0.0 82.20843 1 AGCAGTG 212835 0.0 79.8279 2 CAGTGGT 214920 0.0 79.16143 4 CAACGCA 197620 0.0 44.329517 12-13 ATCAACG 198225 0.0 44.239212 10-11 ACGCAGA 200090 0.0 43.822254 14-15 TCAACGC 196990 0.0 43.519363 12-13 GAGTACT 196805 0.0 43.322678 20-21 AGAGTAC 202555 0.0 43.2289 18-19 AACGCAG 198540 0.0 43.224606 14-15 CGCAGAG 200840 0.0 42.741325 16-17 TATCAAC 202495 0.0 42.321186 10-11 AGTACTT 208870 0.0 41.70681 20-21 GTACTTT 207065 0.0 41.19154 22-23 >>END_MODULE