FastQCFastQC Report
Thu 26 May 2016
SRR1660719_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660719_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26977951
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16281216.03500614260883No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT287630.10661669598258222No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2020350.087.212518
GTGGTAT2044050.086.258156
AGTGGTA2046050.086.2029345
TGGTATC2036750.086.191167
GTATCAA2056600.085.5941549
GCAGTGG2122500.083.65963
AAGCAGT2124050.083.495841
CAGTGGT2157200.081.7463464
AGCAGTG2228300.079.7173542
CAACGCA1995350.045.22339212-13
ATCAACG2004300.045.06501410-11
ACGCAGA2016650.044.7886914-15
TCAACGC1990300.044.41819812-13
AGAGTAC2013650.044.11841218-19
AACGCAG2008700.044.11049714-15
CGCAGAG2022300.043.71085716-17
GAGTACT1987350.043.56640220-21
TATCAAC2037300.043.16306310-11
AGTACTT2071850.042.65279420-21
GTACTTT2087450.041.48872422-23
Produced by FastQC (version 0.11.5)