##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660719_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26977951 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.821533814780818 34.0 31.0 34.0 30.0 34.0 2 31.947873357765385 34.0 31.0 34.0 30.0 34.0 3 32.01180890275914 34.0 31.0 34.0 30.0 34.0 4 35.40933316247776 37.0 35.0 37.0 33.0 37.0 5 35.371761035521196 37.0 35.0 37.0 33.0 37.0 6 35.459015141661425 37.0 35.0 37.0 33.0 37.0 7 35.40573240717948 37.0 35.0 37.0 33.0 37.0 8 35.42078314250033 37.0 35.0 37.0 33.0 37.0 9 37.09795932982457 39.0 37.0 39.0 33.0 39.0 10-11 37.022722111104734 39.0 37.0 39.0 33.0 39.0 12-13 37.060123450442916 39.0 37.0 39.0 33.0 39.0 14-15 38.438662836180555 40.0 38.0 41.0 33.0 41.0 16-17 38.41704151290067 40.0 38.0 41.0 33.0 41.0 18-19 38.381652631810326 40.0 38.0 41.0 33.5 41.0 20-21 38.260994951025005 40.0 38.0 41.0 33.5 41.0 22-23 38.079617480956955 40.0 38.0 41.0 32.5 41.0 24-25 38.071266735564905 40.0 38.0 41.0 33.0 41.0 26-27 38.06730735406852 40.0 38.0 41.0 33.0 41.0 28-29 37.993604332664106 40.0 38.0 41.0 33.0 41.0 30-31 37.91132078562972 40.0 38.0 41.0 32.5 41.0 32-33 37.79292041119061 40.0 38.0 41.0 32.0 41.0 34-35 37.64502854571869 40.0 38.0 41.0 31.5 41.0 36-37 37.45947179235369 40.0 37.0 41.0 31.0 41.0 38-39 37.016987075853166 40.0 35.0 41.0 31.0 41.0 40-41 36.95945429658464 39.5 35.0 41.0 30.5 41.0 42-43 36.96875824631752 40.0 35.0 41.0 30.5 41.0 44-45 36.972391620846224 40.0 35.0 41.0 30.5 41.0 46-47 37.06253226940771 40.0 35.0 41.0 31.0 41.0 48-49 36.95959077841012 40.0 35.0 41.0 30.0 41.0 50-51 35.60404713463969 38.5 34.0 40.0 28.0 40.5 52-53 35.7624954170908 38.5 35.0 39.5 28.5 40.5 54-55 36.11633561422067 39.0 35.0 41.0 27.0 41.0 56-57 35.71950695959082 39.5 35.0 41.0 24.5 41.0 58-59 35.53837493069803 39.0 35.0 41.0 24.0 41.0 60-61 35.33517443559742 39.0 35.0 41.0 24.0 41.0 62-63 35.12529659869276 39.0 35.0 41.0 23.5 41.0 64-65 34.894593106793025 38.0 34.5 40.5 23.0 41.0 66-67 34.65168058908551 37.5 34.0 40.0 23.0 41.0 68-69 34.402444370219214 37.0 34.0 40.0 23.0 41.0 70-71 34.123212489339906 36.5 34.0 39.5 23.0 41.0 72-73 33.792967264267034 36.0 34.0 39.0 23.0 41.0 74-75 33.42215144508195 35.5 33.0 39.0 22.5 40.5 76-77 33.03201742415501 35.0 33.0 37.5 21.0 40.0 78-79 32.6558095905801 35.0 33.0 37.0 20.0 39.0 80-81 32.327263679143016 35.0 33.0 36.5 20.0 39.0 82-83 32.00595085964831 35.0 32.5 36.0 19.5 38.5 84-85 31.7111936707128 35.0 32.0 36.0 19.0 37.0 86-87 31.488616796731524 35.0 32.0 35.0 18.5 37.0 88-89 31.2775804396709 35.0 32.0 35.0 18.0 36.5 90-91 31.09986434848221 35.0 32.0 35.0 18.0 36.0 92-93 30.94510407777077 35.0 31.5 35.0 17.5 36.0 94-95 30.76056611934687 35.0 31.0 35.0 17.0 36.0 96-97 30.628861769376037 35.0 31.0 35.0 15.5 35.0 98-99 30.532587241336454 35.0 31.0 35.0 15.0 35.0 100 30.45907986117997 35.0 31.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5297205953970554 1101 2 0.5489897692629775 1101 3 0.5357482630388688 1101 4 0.43978629182610973 1101 5 0.4833180095735514 1101 6 0.45006824477648166 1101 7 0.4649679915783764 1101 8 0.49193432860366926 1101 9 0.5933296246442694 1101 10-11 0.5978017233296313 1101 12-13 0.5834277343582102 1101 14-15 0.7157696567711724 1101 16-17 0.6791890857513678 1101 18-19 0.6499871149419505 1101 20-21 0.7076854428453174 1101 22-23 0.7301379385168971 1101 24-25 0.74448103118565 1101 26-27 0.7101151383722808 1101 28-29 0.6843810637280185 1101 30-31 0.6611554730611715 1101 32-33 0.6618001700655753 1101 34-35 0.6700601593860469 1101 36-37 0.6195496327141825 1101 38-39 0.6490532570636631 1101 40-41 0.6433056435733135 1101 42-43 0.6541745486208086 1101 44-45 0.6389940873550941 1101 46-47 0.690273498750642 1101 48-49 0.7721396772931328 1101 50-51 0.6693388448762221 1101 52-53 0.6750666603187838 1101 54-55 0.6976181124585921 1101 56-57 0.7031233000821615 1101 58-59 0.6611665469211232 1101 60-61 0.6397604087777822 1101 62-63 0.6032748254034104 1101 64-65 0.5555592805191765 1101 66-67 0.5859021332027226 1101 68-69 0.5795621056541833 1101 70-71 0.5547359248348585 1101 72-73 0.5461804271572746 1101 74-75 0.5245198638617623 1101 76-77 0.4969161563939011 1101 78-79 0.5126944398890529 1101 80-81 0.4114701554263789 1101 82-83 0.4032084317804632 1101 84-85 0.4133024116478161 1101 86-87 0.3858248594140825 1101 88-89 0.4118411128049573 1101 90-91 0.3708786555468855 1101 92-93 0.3617801127831335 1101 94-95 0.3884419112231079 1101 96-97 0.3977293965390949 1101 98-99 0.3322013917911093 1101 100 0.33635719894127547 1102 1 0.43860963754774573 1102 2 0.46805398033182044 1102 3 0.47154323121031894 1102 4 0.4103082807190077 1102 5 0.4387703023960867 1102 6 0.383088803057845 1102 7 0.4000756387691595 1102 8 0.4201122905006187 1102 9 0.47099044693177206 1102 10-11 0.4869783021904581 1102 12-13 0.46745730259669216 1102 14-15 0.56720229845304 1102 16-17 0.5444939803785687 1102 18-19 0.5196852185577185 1102 20-21 0.5460646752728451 1102 22-23 0.5616991563029501 1102 24-25 0.5328099469556165 1102 26-27 0.5138165280123843 1102 28-29 0.5441525307031299 1102 30-31 0.5195249704057687 1102 32-33 0.5130164740588228 1102 34-35 0.5015253501901284 1102 36-37 0.5556850026312503 1102 38-39 0.5413846540921554 1102 40-41 0.5045179759679144 1102 42-43 0.5180096589422618 1102 44-45 0.5486619779700987 1102 46-47 0.5671231479743355 1102 48-49 0.5933699253786742 1102 50-51 0.5388681579518959 1102 52-53 0.5657972421105271 1102 54-55 0.6246482359385581 1102 56-57 0.6542197647986185 1102 58-59 0.5569840320292911 1102 60-61 0.5018434619311449 1102 62-63 0.5007167085461148 1102 64-65 0.4964988880321215 1102 66-67 0.48462949927763077 1102 68-69 0.4450628669483834 1102 70-71 0.47975473581857386 1102 72-73 0.4350966992996277 1102 74-75 0.47213112513609445 1102 76-77 0.4351263772632521 1102 78-79 0.43456296046660725 1102 80-81 0.3805132507904574 1102 82-83 0.3812519482715899 1102 84-85 0.3653728792634432 1102 86-87 0.33943689133490196 1102 88-89 0.35211343510587767 1102 90-91 0.32945444245417477 1102 92-93 0.29934076097566376 1102 94-95 0.3484237857063128 1102 96-97 0.33581741589503267 1102 98-99 0.2608747931101689 1102 100 0.2980872585368495 1103 1 0.38237835547682053 1103 2 0.39969978016948104 1103 3 0.4188303018123847 1103 4 0.35339071467014094 1103 5 0.3920309766870673 1103 6 0.3708893500258412 1103 7 0.360856417048268 1103 8 0.4035419899752526 1103 9 0.4605795194960294 1103 10-11 0.45988440515986184 1103 12-13 0.4601519974435462 1103 14-15 0.5439369786617902 1103 16-17 0.5301796602641673 1103 18-19 0.4963707554725474 1103 20-21 0.5253203104251725 1103 22-23 0.5445227979128902 1103 24-25 0.587475209707911 1103 26-27 0.5529416815029151 1103 28-29 0.5384738421322623 1103 30-31 0.5080748763841854 1103 32-33 0.4470043242648458 1103 34-35 0.467723363223719 1103 36-37 0.4791476413890976 1103 38-39 0.5071292793603632 1103 40-41 0.49015619340063665 1103 42-43 0.5325953905163985 1103 44-45 0.5218319229033028 1103 46-47 0.5614899264616611 1103 48-49 0.5765703750026958 1103 50-51 0.48375114873640257 1103 52-53 0.4823615079508201 1103 54-55 0.600819821212724 1103 56-57 0.6225848643322038 1103 58-59 0.5522132761599536 1103 60-61 0.5595092412420186 1103 62-63 0.5194252524091851 1103 64-65 0.5322005074398035 1103 66-67 0.5117198474754829 1103 68-69 0.491059171287624 1103 70-71 0.47743126080872145 1103 72-73 0.4352520334397596 1103 74-75 0.47945952713656226 1103 76-77 0.47834657358024657 1103 78-79 0.4520117609628116 1103 80-81 0.3746630294460047 1103 82-83 0.3890172002484036 1103 84-85 0.3540848611977161 1103 86-87 0.2941673262610571 1103 88-89 0.33835339012336973 1103 90-91 0.31788919105058255 1103 92-93 0.30049777534357247 1103 94-95 0.39451574070913864 1103 96-97 0.37278130827826317 1103 98-99 0.31507532922940484 1103 100 0.30983754655419204 1104 1 0.37528244923602827 1104 2 0.4268933664542196 1104 3 0.41216285204278336 1104 4 0.3623715788151358 1104 5 0.3424550284531591 1104 6 0.30210627565877246 1104 7 0.3088187059491574 1104 8 0.32578796636409635 1104 9 0.38324636227124387 1104 10-11 0.3518478887805898 1104 12-13 0.36807334980112216 1104 14-15 0.4411581807416596 1104 16-17 0.44520570122455894 1104 18-19 0.40889297530478785 1104 20-21 0.37736133849422515 1104 22-23 0.42428648548420966 1104 24-25 0.4129914061656734 1104 26-27 0.3957967978088277 1104 28-29 0.3804355760841176 1104 30-31 0.34592475821980884 1104 32-33 0.3264333760482643 1104 34-35 0.3405926949918552 1104 36-37 0.30384863635330817 1104 38-39 0.3088516501473535 1104 40-41 0.32290732458213256 1104 42-43 0.33847409938131534 1104 44-45 0.3371536265407542 1104 46-47 0.35542023553083624 1104 48-49 0.38826933978680955 1104 50-51 0.35058588268100266 1104 52-53 0.3667022921255878 1104 54-55 0.4799792159011034 1104 56-57 0.4895539190486389 1104 58-59 0.43967866212077666 1104 60-61 0.4568077347452473 1104 62-63 0.4406380173016444 1104 64-65 0.42324611184877625 1104 66-67 0.4386845774365895 1104 68-69 0.39405753472993155 1104 70-71 0.3968662947538206 1104 72-73 0.3795792682677259 1104 74-75 0.37338921857794816 1104 76-77 0.37194812162699264 1104 78-79 0.3767185684650727 1104 80-81 0.33826658231061657 1104 82-83 0.3150401681171999 1104 84-85 0.30048410123209734 1104 86-87 0.2681320551203292 1104 88-89 0.24083665164660673 1104 90-91 0.24990499385275555 1104 92-93 0.2645822058805827 1104 94-95 0.32436380765492245 1104 96-97 0.3248392519150052 1104 98-99 0.3013228298280808 1104 100 0.27588540459110433 1105 1 0.2972466327697525 1105 2 0.3439044596288632 1105 3 0.32880486604639714 1105 4 0.2731165935154536 1105 5 0.28434234206555686 1105 6 0.27604736335072033 1105 7 0.2885827227276323 1105 8 0.26208888926402807 1105 9 0.3010887252877339 1105 10-11 0.32103234052930674 1105 12-13 0.31305973509672924 1105 14-15 0.377062977618678 1105 16-17 0.3353145477821897 1105 18-19 0.3399334912644534 1105 20-21 0.35332862070684 1105 22-23 0.3825914252344518 1105 24-25 0.42570864513324835 1105 26-27 0.3883812655387544 1105 28-29 0.36440960556975455 1105 30-31 0.39472115086557835 1105 32-33 0.37857512384800174 1105 34-35 0.3578716866732421 1105 36-37 0.3426570156550639 1105 38-39 0.3675929794428825 1105 40-41 0.3467382571426256 1105 42-43 0.3728862055671769 1105 44-45 0.42883876961148815 1105 46-47 0.4306809813984671 1105 48-49 0.475907181467484 1105 50-51 0.34404550124482114 1105 52-53 0.39745951943346114 1105 54-55 0.486581921065131 1105 56-57 0.48638535977570285 1105 58-59 0.45868745522814436 1105 60-61 0.46277190226783915 1105 62-63 0.4174782057961366 1105 64-65 0.4130722121880339 1105 66-67 0.384935505932944 1105 68-69 0.4098235300062001 1105 70-71 0.43117870542065617 1105 72-73 0.39144988091670285 1105 74-75 0.3911385374312246 1105 76-77 0.34407144015825253 1105 78-79 0.3754604035330047 1105 80-81 0.34810110570543173 1105 82-83 0.3140615434350238 1105 84-85 0.33075508375332063 1105 86-87 0.29748780284943876 1105 88-89 0.32350398898541144 1105 90-91 0.2882061792156456 1105 92-93 0.28938807831700686 1105 94-95 0.3104021516364064 1105 96-97 0.32333852572760335 1105 98-99 0.2580961059822009 1105 100 0.2702105037105227 1106 1 0.2306217634357246 1106 2 0.2696393607551677 1106 3 0.2640297836146317 1106 4 0.2734906190137849 1106 5 0.268027250718589 1106 6 0.24861963665110665 1106 7 0.2527682670039937 1106 8 0.25296836796290734 1106 9 0.28250347193599623 1106 10-11 0.25217119191113824 1106 12-13 0.2660230176959004 1106 14-15 0.331972707652028 1106 16-17 0.319905544739008 1106 18-19 0.28743015828875684 1106 20-21 0.27216531839674474 1106 22-23 0.2844976830942727 1106 24-25 0.2780470627227558 1106 26-27 0.30878987968120697 1106 28-29 0.31246861618641475 1106 30-31 0.2994644855862205 1106 32-33 0.31083417880695663 1106 34-35 0.297779957379241 1106 36-37 0.284650478733397 1106 38-39 0.287688271371465 1106 40-41 0.2937444548937691 1106 42-43 0.33673847784023536 1106 44-45 0.33285630628795104 1106 46-47 0.3420552065480962 1106 48-49 0.3563365096141524 1106 50-51 0.251796723082677 1106 52-53 0.30766101603217777 1106 54-55 0.4405378121544601 1106 56-57 0.46376568353745995 1106 58-59 0.3865097898001153 1106 60-61 0.37445211427981206 1106 62-63 0.35737442160208843 1106 64-65 0.3231468967606119 1106 66-67 0.3557262186971144 1106 68-69 0.3446887217018215 1106 70-71 0.341525440810841 1106 72-73 0.29559627857977233 1106 74-75 0.30781121146006996 1106 76-77 0.336750410901935 1106 78-79 0.3519527443507613 1106 80-81 0.3150507151859827 1106 82-83 0.31898830504139397 1106 84-85 0.3123088872492268 1106 86-87 0.24751023111405246 1106 88-89 0.29320380232475074 1106 90-91 0.2764599687625555 1106 92-93 0.25869005073620244 1106 94-95 0.32007617203315775 1106 96-97 0.3520576285724779 1106 98-99 0.2921750702743964 1106 100 0.24109432401419184 1107 1 0.1918962273699698 1107 2 0.20755323610984178 1107 3 0.2175513863668428 1107 4 0.24512934956873522 1107 5 0.24456894435292043 1107 6 0.1960502643564297 1107 7 0.2122920707483047 1107 8 0.22936451399420577 1107 9 0.234862806717949 1107 10-11 0.21381982928956944 1107 12-13 0.24225585515928572 1107 14-15 0.2684464815787777 1107 16-17 0.26104548483446166 1107 18-19 0.225144020221002 1107 20-21 0.26087234989607566 1107 22-23 0.280387652224384 1107 24-25 0.28975506685287655 1107 26-27 0.26525795984261435 1107 28-29 0.26924963463467577 1107 30-31 0.23177221408120374 1107 32-33 0.1799347973910983 1107 34-35 0.24866272519877697 1107 36-37 0.22140656482118004 1107 38-39 0.23326498255154604 1107 40-41 0.23370035915304044 1107 42-43 0.29384151506096146 1107 44-45 0.2781912905647985 1107 46-47 0.26519484120282044 1107 48-49 0.3267420719312568 1107 50-51 0.26882096179114257 1107 52-53 0.24854928376783647 1107 54-55 0.3093596741894018 1107 56-57 0.3780241109443594 1107 58-59 0.3200055541946085 1107 60-61 0.34685410136083306 1107 62-63 0.2976181560584692 1107 64-65 0.29617357749063444 1107 66-67 0.317319285965155 1107 68-69 0.32836578298317676 1107 70-71 0.30046343357290795 1107 72-73 0.28535520365266365 1107 74-75 0.2541048365523224 1107 76-77 0.2814825001654384 1107 78-79 0.26672825686571144 1107 80-81 0.2126740485057539 1107 82-83 0.21670402956721802 1107 84-85 0.21841308985592534 1107 86-87 0.1835439126044669 1107 88-89 0.17596371062689187 1107 90-91 0.21806776925525995 1107 92-93 0.20577116863560363 1107 94-95 0.20508982883052695 1107 96-97 0.2828696821102845 1107 98-99 0.269189113265913 1107 100 0.2573840451130245 1108 1 0.11955682764035913 1108 2 0.14370860633985316 1108 3 0.1624845913051729 1108 4 0.16238765416209588 1108 5 0.15833676351468995 1108 6 0.08466081241866164 1108 7 0.10155712049400023 1108 8 0.13705753963390777 1108 9 0.14369185578136978 1108 10-11 0.09978644631071631 1108 12-13 0.10652163250392732 1108 14-15 0.16244827021588293 1108 16-17 0.13091545710242514 1108 18-19 0.10186612323381183 1108 20-21 0.09421099549966527 1108 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-1.2092378464088647 2316 92-93 -1.1304777011113991 2316 94-95 -1.3285432125249166 2316 96-97 -1.4609592878048296 2316 98-99 -1.335872533466521 2316 100 -1.1640256538891798 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13800.0 3 3584.0 4 2397.0 5 2116.0 6 3546.0 7 18245.0 8 34685.0 9 38190.0 10 38456.0 11 37521.0 12 38294.0 13 41278.0 14 47522.0 15 55805.0 16 66434.0 17 79188.0 18 89819.0 19 102023.0 20 118634.0 21 144772.0 22 187039.0 23 269642.0 24 409252.0 25 526892.0 26 522262.0 27 437192.0 28 381786.0 29 366930.0 30 401348.0 31 482009.0 32 600660.0 33 773978.0 34 1066776.0 35 1622027.0 36 2817813.0 37 5387843.0 38 7312920.0 39 2430734.0 40 4539.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 15.635158422069296 34.39753834472851 28.713783259702026 21.253519973500172 2 20.05243958769494 34.79843066006743 27.06326959314758 18.085860159090046 3 25.936274059875558 25.70881932804186 27.442909638326963 20.911996973755617 4 19.020423228437828 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24-25 19.878750328751842 30.14353624950401 26.479998262116244 23.49771515962791 26-27 19.891063233591836 26.712070373061316 33.511921014305415 19.884945379041437 28-29 19.867292445985868 26.81346403849841 33.49888229221909 19.820361223296633 30-31 19.836053432309487 26.74396729565844 33.4956588781005 19.924320393931577 32-33 19.953438862165882 26.86834538738202 33.29459948538256 19.88361626506954 34-35 19.965699710161104 26.994869483627166 33.178511768985 19.860919037226733 36-37 19.924905643197093 26.920711343747218 33.24640090933691 19.907982103718773 38-39 19.93973183583363 26.94053689306386 33.24028503856961 19.8794462325329 40-41 19.957827605751618 26.939819469856452 33.24088135654726 19.861471567844664 42-43 19.936530278233015 26.99037501934063 33.18014447643192 19.89295022599444 44-45 19.952045347441725 27.048820199077557 33.13367714094795 19.86545731253277 46-47 19.922729928000578 27.13376302330949 33.10588212074537 19.837624927944557 48-49 20.001251400846414 27.114060957690427 33.00386084801013 19.880826793453025 50-51 19.97235338819318 27.182339740203997 32.95422252266623 19.891084348936598 52-53 20.07467228440099 27.34443611128386 32.67387861888278 19.90701298543237 54-55 20.42574357338427 27.61712481785455 31.847255548465895 20.10987606029528 56-57 20.88820946001732 28.20598865899257 30.602307571995436 20.303494308994676 58-59 20.954850438746863 28.776891296602606 29.913523387306086 20.354734877344445 60-61 21.036190521283032 29.078655156675115 29.547841258770152 20.3373130632717 62-63 20.99056694792258 29.260286081884328 29.379733632648286 20.3694133375448 64-65 21.03592233852794 29.314460664306015 29.361863327880368 20.287753669285678 66-67 21.05786075971729 29.105974500717107 29.602130591375346 20.234034148190254 68-69 21.08144837190106 28.872075872543153 29.886835506556785 20.159640248999 70-71 21.102527867694786 28.72549292327949 30.09682272936996 20.075156479655764 72-73 21.181722470685468 28.66840883385231 30.105334014099522 20.0445346813627 74-75 21.291120193794367 28.770903562632842 29.956722899832155 19.981253343740637 76-77 21.521232327237154 28.931372164099216 29.606071608473115 19.941323900190515 78-79 21.74715066942043 29.10168090469944 29.22471040697258 19.926458018907546 80-81 21.96879002963226 29.269502910413607 28.881882531735247 19.879824528218887 82-83 22.134281381013633 29.432042318969888 28.54153136898133 19.892144931035148 84-85 22.243832801950578 29.500543629162753 28.353305739579476 19.902317829307197 86-87 22.384244890310356 29.54903319903885 28.1970315485857 19.8696903620651 88-89 22.582041948784692 29.630013185922998 27.97962922627688 19.808315639015426 90-91 22.809801808078248 29.598529105899257 27.836168981694154 19.75550010432834 92-93 22.988757078388677 29.60523982327607 27.75370916670439 19.652293931630865 94-95 23.201554412049248 29.628876087428306 27.59360692239457 19.575962578127868 96-97 23.35466001553292 29.617437327817942 27.48314715169466 19.544755504954484 98-99 23.518757949986245 29.62661503507857 27.35619564397259 19.498431370962592 100 23.589068253460475 29.63147050331518 27.257614747266175 19.521846495958172 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 37714.0 1 27211.0 2 14106.0 3 10781.5 4 9970.5 5 9830.0 6 9908.5 7 9849.5 8 9717.0 9 9824.5 10 10485.5 11 13140.0 12 117445.0 13 148041.0 14 75329.0 15 79955.5 16 90964.0 17 100837.5 18 109600.0 19 118436.5 20 131018.5 21 152097.5 22 181619.0 23 214672.0 24 247284.0 25 282917.5 26 320689.5 27 365234.5 28 429289.5 29 490315.5 30 532449.0 31 580856.0 32 633237.5 33 668037.5 34 695640.0 35 738852.5 36 781732.0 37 804949.5 38 828162.0 39 841105.5 40 841585.0 41 860486.0 42 895871.0 43 890865.5 44 859647.5 45 849977.0 46 842176.0 47 832335.5 48 813814.0 49 791404.0 50 776561.5 51 761040.0 52 742850.0 53 712966.0 54 666412.0 55 613341.0 56 560752.0 57 508643.0 58 454094.5 59 391199.0 60 324351.5 61 268099.5 62 214378.5 63 157660.0 64 111524.5 65 78542.0 66 55003.5 67 40963.0 68 30989.0 69 22615.5 70 16734.5 71 13074.0 72 10947.0 73 9408.0 74 8621.0 75 8354.5 76 6675.5 77 3940.0 78 2451.0 79 1987.5 80 1682.0 81 1427.0 82 1224.5 83 1044.5 84 902.5 85 788.5 86 671.5 87 586.5 88 501.5 89 422.0 90 340.0 91 292.5 92 274.5 93 225.5 94 175.5 95 158.5 96 141.0 97 117.0 98 88.5 99 57.0 100 31.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4071880774043959 2 0.38825039010560886 3 0.16887494532108832 4 0.17548033948167524 5 0.2758549009151955 6 0.10282471044594899 7 0.13751229661585493 8 0.14317988790179062 9 0.13620011393748915 10-11 0.13261385195636244 12-13 0.13450984472467906 14-15 0.14091322206048934 16-17 0.14519634941882725 18-19 0.1570912483309055 20-21 0.13807571968679164 22-23 0.0999631143225073 24-25 0.09500536197133726 26-27 0.08917467453328831 28-29 0.09937745086719152 30-31 0.103380720055426 32-33 0.10461506138846498 34-35 0.10499129455754441 36-37 0.10128271046233274 38-39 0.10034861431841136 40-41 0.10149955421002876 42-43 0.0638354632640559 44-45 0.06711406659460535 46-47 0.08530299428596337 48-49 0.08954534760627299 50-51 0.09907349894734407 52-53 0.1040071575487701 54-55 0.10629977050518032 56-57 0.10692435463315951 58-59 0.10616632819890584 60-61 0.10412391956676027 62-63 0.10861833057669948 64-65 0.11547578242691596 66-67 0.12420142656497522 68-69 0.1292574072804862 70-71 0.1276320058554484 72-73 0.11439897714989547 74-75 0.11198774881013016 76-77 0.11377068629118646 78-79 0.1124974242854841 80-81 0.10665932338597545 82-83 0.10993421998579506 84-85 0.11592059011449757 86-87 0.12991164525430415 88-89 0.1375160033465848 90-91 0.14442164269628927 92-93 0.14324846242029277 94-95 0.133664710118274 96-97 0.13038796015308945 98-99 0.12499837367189227 100 0.11939750353909384 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.6977951E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.90600123723448 #Duplication Level Percentage of deduplicated Percentage of total 1 76.84381545997108 30.66529394819424 2 11.310068565965024 9.02679220373214 3 3.913135810469502 4.684728074821875 4 1.9933359718179322 3.181842710303616 5 1.2122779593561845 2.4188582872969997 6 0.8166078097390684 1.955253135946956 7 0.5888096060343437 1.6447925806831447 8 0.4511942696856263 1.4404287267446167 9 0.342450597257841 1.2299250562076778 >10 2.1319212350929204 16.08727419291048 >50 0.20856019237844467 5.760949311951267 >100 0.1846923754174908 14.482050779290315 >500 0.002488541948450088 0.6184214356438192 >1k 5.859031830145528E-4 0.4775373255424439 >5k 1.8567227649668973E-5 0.04608300023556563 >10k+ 3.713445529925399E-5 6.279769230494786 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1628121 6.03500614260883 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 28763 0.10661669598258222 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.408557677341767E-4 0.0 0.0 3.706730729846755E-6 3.706730729846755E-6 2 1.482692291938702E-4 7.41346145969351E-6 0.0 3.706730729846755E-6 7.41346145969351E-6 3 1.5568269065356372E-4 1.482692291938702E-5 0.0 3.706730729846755E-6 7.41346145969351E-6 4 1.6680288284310398E-4 2.965384583877404E-5 0.0 3.706730729846755E-6 7.41346145969351E-6 5 1.7050961357295075E-4 3.3360576568620795E-5 0.0 3.706730729846755E-6 7.41346145969351E-6 6 1.7792307503264424E-4 4.077403802831431E-5 0.0 3.706730729846755E-6 1.8533653649233776E-5 7 1.7792307503264424E-4 4.077403802831431E-5 0.0 3.706730729846755E-6 2.224038437908053E-5 8 1.8533653649233775E-4 4.077403802831431E-5 0.0 3.706730729846755E-6 2.5947115108927287E-5 9 2.0016345941172478E-4 4.448076875816106E-5 0.0 3.706730729846755E-6 2.5947115108927287E-5 10-11 2.1128365160126504E-4 4.818749948800782E-5 0.0 1.2973557554463644E-5 2.5947115108927287E-5 12-13 2.4093749744003907E-4 5.374759558277795E-5 0.0 1.482692291938702E-5 3.1507211203697416E-5 14-15 2.5947115108927287E-4 5.74543263126247E-5 0.0 1.482692291938702E-5 3.5213941933544173E-5 16-17 2.817115354683534E-4 5.930769167754808E-5 0.0 1.6680288284310397E-5 4.448076875816106E-5 18-19 3.0024518911758713E-4 6.857451850216496E-5 0.0 1.8533653649233776E-5 5.374759558277795E-5 20-21 3.224855734966677E-4 7.969471069170525E-5 0.0 1.8533653649233776E-5 6.857451850216496E-5 22-23 3.502860539705184E-4 8.154807605662862E-5 0.0 1.8533653649233776E-5 8.154807605662862E-5 24-25 4.299807646622236E-4 9.266826824616888E-5 0.0 1.8533653649233776E-5 8.525480678647538E-5 26-27 6.57944704547799E-4 1.0378846043570915E-4 0.0 1.8533653649233776E-5 8.896153751632212E-5 28-29 0.0013381297934746787 1.0934855653047928E-4 0.0 1.8533653649233776E-5 9.822836434093902E-5 30-31 0.0030561994867586498 1.1861538335509616E-4 0.0 1.8533653649233776E-5 1.074951911655559E-4 32-33 0.006692502332738316 1.2232211408494292E-4 0.0 1.8533653649233776E-5 1.0934855653047928E-4 34-35 0.013881706583276098 1.2417547944986628E-4 0.0 1.8533653649233776E-5 1.334423062744832E-4 36-37 0.025913754532358668 1.2602884481478966E-4 0.0 1.8533653649233776E-5 1.482692291938702E-4 38-39 0.04448632885425584 1.3158894090955982E-4 0.0 2.965384583877404E-5 1.649495174781806E-4 40-41 0.07145650164462083 1.3714903700432995E-4 0.0 3.3360576568620795E-5 1.6680288284310398E-4 42-43 0.10943381133726575 1.649495174781806E-4 0.0 3.3360576568620795E-5 1.7977644039756762E-4 44-45 0.159691519937893 1.7236297893787414E-4 0.0 3.3360576568620795E-5 1.9089663258710788E-4 46-47 0.22581588942762926 1.834831711274144E-4 0.0 3.3360576568620795E-5 2.1313701696618843E-4 48-49 0.31018478756967127 2.0387019014157153E-4 0.0 3.3360576568620795E-5 2.242572091557287E-4 50-51 0.4141141037731146 2.1869711306095855E-4 0.0 3.3360576568620795E-5 2.4279086280496246E-4 52-53 0.5362786818020391 2.2611057452065207E-4 0.0 3.3360576568620795E-5 2.539110549945027E-4 54-55 0.6807207115173424 2.50204324264656E-4 0.0 3.3360576568620795E-5 2.6873797791388974E-4 56-57 0.8519976183513714 2.613245164541962E-4 0.0 3.3360576568620795E-5 2.7985817010343006E-4 58-59 1.05352144794095 2.780048047385066E-4 0.0 4.262740339323769E-5 2.9097836229297026E-4 60-61 1.2859353180677064 2.817115354683534E-4 0.0 4.448076875816106E-5 2.9283172765789365E-4 62-63 1.54321579129564 2.928317276578937E-4 0.0 4.448076875816106E-5 2.9283172765789365E-4 64-65 1.8291103723926252 3.0951201594220406E-4 0.0 4.448076875816106E-5 3.0209855448251057E-4 66-67 2.145743018066865 3.18778842766821E-4 0.0 4.448076875816106E-5 3.243389388615911E-4 68-69 2.4967055503955806 3.261923042265144E-4 0.0 4.448076875816106E-5 3.317524003212846E-4 70-71 2.8816143227482325 3.447259578757483E-4 0.0 4.448076875816106E-5 3.391658617809781E-4 72-73 3.2967051500686617 3.669663422548288E-4 0.0 4.448076875816106E-5 3.5399278470036515E-4 74-75 3.7442799121401027 3.8179326517421576E-4 0.0 4.448076875816106E-5 3.6511297688990535E-4 76-77 4.222451883021064 3.966201880936028E-4 0.0 4.448076875816106E-5 3.743798037145223E-4 78-79 4.7337323727810166 4.0403364955329634E-4 0.0 4.818749948800782E-5 3.8364663053913915E-4 80-81 5.277461583350048 4.114471110129898E-4 0.0 4.818749948800782E-5 3.929134573637561E-4 82-83 5.850442459473665 4.1700720710776E-4 0.0 4.818749948800782E-5 4.058870149182197E-4 84-85 6.452734308843544 4.2812739929730024E-4 0.0 4.818749948800782E-5 4.114471110129898E-4 86-87 7.088407121801059 4.411009568517639E-4 0.0 4.818749948800782E-5 4.133004763779132E-4 88 7.583485491540851 4.559278797711509E-4 0.0 4.818749948800782E-5 4.1886057247268336E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 202035 0.0 87.21251 8 GTGGTAT 204405 0.0 86.25815 6 AGTGGTA 204605 0.0 86.202934 5 TGGTATC 203675 0.0 86.19116 7 GTATCAA 205660 0.0 85.594154 9 GCAGTGG 212250 0.0 83.6596 3 AAGCAGT 212405 0.0 83.49584 1 CAGTGGT 215720 0.0 81.746346 4 AGCAGTG 222830 0.0 79.717354 2 CAACGCA 199535 0.0 45.223392 12-13 ATCAACG 200430 0.0 45.065014 10-11 ACGCAGA 201665 0.0 44.78869 14-15 TCAACGC 199030 0.0 44.418198 12-13 AGAGTAC 201365 0.0 44.118412 18-19 AACGCAG 200870 0.0 44.110497 14-15 CGCAGAG 202230 0.0 43.710857 16-17 GAGTACT 198735 0.0 43.566402 20-21 TATCAAC 203730 0.0 43.163063 10-11 AGTACTT 207185 0.0 42.652794 20-21 GTACTTT 208745 0.0 41.488724 22-23 >>END_MODULE