FastQCFastQC Report
Thu 26 May 2016
SRR1660719_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660719_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26977951
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16044835.947386441616712No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT346010.12825658998342757No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT313620.11625048914945393No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2068300.086.748578
TGGTATC2088450.085.845727
GTGGTAT2071500.085.82036
AGTGGTA2082250.085.431345
GTATCAA2100600.085.335849
GCAGTGG2094500.084.943053
AAGCAGT2041400.084.895731
CAGTGGT2183200.081.4072954
AGCAGTG2197100.080.703222
CAACGCA2030550.045.11536812-13
ATCAACG2040800.044.93362410-11
ACGCAGA2054350.044.6339514-15
TCAACGC2026300.044.25098812-13
AGAGTAC2073100.044.10387418-19
AACGCAG2041550.043.96999414-15
CGCAGAG2059950.043.52845416-17
GAGTACT2047050.043.45006620-21
TATCAAC2081700.043.0214810-11
AGTACTT2126100.042.73313520-21
GTACTTT2150200.041.34316622-23
Produced by FastQC (version 0.11.5)