##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660719_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26977951 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.21773510523464 34.0 31.0 34.0 27.0 34.0 2 31.872487721547127 34.0 31.0 34.0 28.0 34.0 3 31.659954456882215 34.0 31.0 34.0 30.0 34.0 4 35.57836775669138 37.0 35.0 37.0 33.0 37.0 5 35.90863001419196 37.0 35.0 37.0 35.0 37.0 6 35.37232334657291 37.0 35.0 37.0 33.0 37.0 7 35.57896150081969 37.0 35.0 37.0 35.0 37.0 8 35.953609115829444 37.0 35.0 37.0 35.0 37.0 9 37.76887855567682 39.0 38.0 39.0 35.0 39.0 10-11 37.77437037379154 39.0 37.5 39.0 35.0 39.0 12-13 37.862472765259305 39.0 38.0 39.0 35.0 39.0 14-15 39.32190074405577 41.0 39.0 41.0 36.0 41.0 16-17 39.29009636424945 41.0 39.0 41.0 36.0 41.0 18-19 39.28653640152286 41.0 39.0 41.0 36.0 41.0 20-21 39.222298869176534 40.5 39.0 41.0 36.0 41.0 22-23 39.08900032845341 40.0 39.0 41.0 35.5 41.0 24-25 39.10952321768247 40.0 39.0 41.0 36.0 41.0 26-27 39.075555793692416 40.0 39.0 41.0 35.5 41.0 28-29 39.0060551485174 40.0 39.0 41.0 35.0 41.0 30-31 38.91831321807946 40.0 38.5 41.0 35.0 41.0 32-33 38.80439064849662 40.0 38.0 41.0 35.0 41.0 34-35 38.72432850812132 40.0 38.0 41.0 35.0 41.0 36-37 38.5985128373908 40.0 38.0 41.0 35.0 41.0 38-39 38.17433533035923 40.0 37.0 41.0 34.0 41.0 40-41 38.04950683615668 40.0 37.0 41.0 33.5 41.0 42-43 37.89329006120591 40.0 37.0 41.0 33.0 41.0 44-45 37.775086495634895 40.0 36.0 41.0 33.0 41.0 46-47 37.63198963850145 40.0 36.0 41.0 33.0 41.0 48-49 37.67940224593039 40.0 36.0 41.0 33.0 41.0 50-51 37.68069107620516 40.0 36.0 41.0 33.0 41.0 52-53 37.425603616078924 40.0 36.0 41.0 32.5 41.0 54-55 36.87077536021917 40.0 35.5 41.0 30.5 41.0 56-57 36.334868333773755 39.5 35.0 41.0 28.5 41.0 58-59 36.189037392053976 39.0 35.0 41.0 28.0 41.0 60-61 36.0555354630157 39.0 35.0 41.0 28.0 41.0 62-63 35.836610108010056 39.0 35.0 41.0 28.0 41.0 64-65 35.590133679907716 38.5 35.0 40.5 28.0 41.0 66-67 35.33150673674216 37.5 35.0 40.0 28.0 41.0 68-69 35.08561039346539 37.0 35.0 40.0 27.5 41.0 70-71 34.80324456442226 36.5 34.5 39.5 27.0 41.0 72-73 34.47051141875082 36.0 34.0 39.0 26.0 41.0 74-75 34.09600421470111 35.5 34.0 39.0 26.0 40.5 76-77 32.37984678673335 34.5 32.0 36.5 24.0 39.0 78-79 33.31627222171172 35.0 33.5 37.0 26.0 39.0 80-81 33.20888478520848 35.0 34.0 37.0 26.0 39.0 82-83 32.937309045449744 35.0 34.0 36.0 25.0 38.5 84-85 32.675936248827796 35.0 34.0 36.0 25.0 37.0 86-87 32.45309512201278 35.0 34.0 35.5 25.0 37.0 88-89 32.27065079923972 35.0 33.0 35.0 24.0 36.5 90-91 32.103861909305124 35.0 33.0 35.0 24.0 36.0 92-93 31.956458720678974 35.0 33.0 35.0 24.0 36.0 94-95 31.84757702688392 35.0 33.0 35.0 23.0 36.0 96-97 31.74654466901508 35.0 33.0 35.0 23.0 36.0 98-99 31.65827578973659 35.0 33.0 35.0 22.0 35.0 100 31.604942791985945 35.0 33.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.5909123882705138 1101 2 1.180623958753607 1101 3 1.5319499022735492 1101 4 0.9458313883636791 1101 5 0.5706682906777303 1101 6 1.1210981870255168 1101 7 0.8362559045671389 1101 8 0.49791825107752175 1101 9 0.5352846937523026 1101 10-11 0.43956937674639107 1101 12-13 0.3918682935619344 1101 14-15 0.5353753487807538 1101 16-17 0.5523058127204834 1101 18-19 0.5265324445766879 1101 20-21 0.5174429261309754 1101 22-23 0.5054171908084442 1101 24-25 0.5003439406406969 1101 26-27 0.5184074811733126 1101 28-29 0.4940318867000286 1101 30-31 0.49475238693206336 1101 32-33 0.5481196110446405 1101 34-35 0.5813802529521723 1101 36-37 0.5504614851381646 1101 38-39 0.5671155494588973 1101 40-41 0.584474891426737 1101 42-43 0.5694600521854696 1101 44-45 0.5582935143263725 1101 46-47 0.5671313237313811 1101 48-49 0.5586121360382847 1101 50-51 0.5528265362666858 1101 52-53 0.5344926209942997 1101 54-55 0.5595804924827732 1101 56-57 0.6137983030015732 1101 58-59 0.5753900739828097 1101 60-61 0.6079912579949962 1101 62-63 0.4966573209110834 1101 64-65 0.5061215075485492 1101 66-67 0.5253116485983398 1101 68-69 0.5168310503071041 1101 70-71 0.49529635510377545 1101 72-73 0.4922686306229096 1101 74-75 0.486746927122347 1101 76-77 0.3886069402070689 1101 78-79 0.4961549461358814 1101 80-81 0.49136521027683244 1101 82-83 0.49939713613175485 1101 84-85 0.4740127117607642 1101 86-87 0.4696261298474056 1101 88-89 0.46412257065104257 1101 90-91 0.47471297461038375 1101 92-93 0.48049503241826486 1101 94-95 0.4308225433372108 1101 96-97 0.419739662984707 1101 98-99 0.4078261487253876 1101 100 0.4179744274818198 1102 1 1.5020128716720826 1102 2 1.1349335429264258 1102 3 1.4774320359838207 1102 4 0.8957944119272909 1102 5 0.4852459027307674 1102 6 1.0453252106608986 1102 7 0.7730001820969647 1102 8 0.42770367005862653 1102 9 0.46579842097838764 1102 10-11 0.3557845009496958 1102 12-13 0.3466133794300319 1102 14-15 0.4518424307590365 1102 16-17 0.4665411039579439 1102 18-19 0.4024304809927912 1102 20-21 0.3848811397519043 1102 22-23 0.4025025819768828 1102 24-25 0.3807367667423023 1102 26-27 0.37820616276490426 1102 28-29 0.4041680784312547 1102 30-31 0.4015206137200735 1102 32-33 0.42203498252420957 1102 34-35 0.3967139989166242 1102 36-37 0.37934070798164754 1102 38-39 0.3883897467900823 1102 40-41 0.41459070472651405 1102 42-43 0.44088042075079414 1102 44-45 0.392758836085072 1102 46-47 0.43837765611030477 1102 48-49 0.3979900790373492 1102 50-51 0.35393096922161504 1102 52-53 0.3404638238319109 1102 54-55 0.3605258093842778 1102 56-57 0.3532543773019938 1102 58-59 0.37226341555237497 1102 60-61 0.399913947388562 1102 62-63 0.3418337973687571 1102 64-65 0.32887169012728634 1102 66-67 0.3211491728667326 1102 68-69 0.30389851815353097 1102 70-71 0.2846804767167157 1102 72-73 0.2980240355986439 1102 74-75 0.2987904714583536 1102 76-77 0.15199508867883083 1102 78-79 0.32475542618826836 1102 80-81 0.322937682461351 1102 82-83 0.31479909528228944 1102 84-85 0.32395003335332717 1102 86-87 0.3066498968815594 1102 88-89 0.31537912423961245 1102 90-91 0.33724287712598056 1102 92-93 0.3305165585044101 1102 94-95 0.3091872629607231 1102 96-97 0.2890676206837277 1102 98-99 0.28015234939857336 1102 100 0.31995064049950983 1103 1 1.4810140707171406 1103 2 1.0903618221158382 1103 3 1.4321143327818326 1103 4 0.8889914796780118 1103 5 0.48885850375561546 1103 6 1.0386375684684879 1103 7 0.742579466560521 1103 8 0.4282990972406395 1103 9 0.42986830559443945 1103 10-11 0.3278528281000064 1103 12-13 0.2974281663868297 1103 14-15 0.3616836565795367 1103 16-17 0.3978674420718633 1103 18-19 0.36718469548823407 1103 20-21 0.3955936248349232 1103 22-23 0.3551966142578067 1103 24-25 0.3526277875661705 1103 26-27 0.3951983561535144 1103 28-29 0.3621248557009267 1103 30-31 0.3462532487468053 1103 32-33 0.3994118424255859 1103 34-35 0.41383389932283876 1103 36-37 0.3744561882555999 1103 38-39 0.39479226368728604 1103 40-41 0.4367517207280045 1103 42-43 0.43361537670534744 1103 44-45 0.37057640847052653 1103 46-47 0.4007280552592718 1103 48-49 0.3385538899592646 1103 50-51 0.3238454870226164 1103 52-53 0.3108111401936071 1103 54-55 0.33662039315925796 1103 56-57 0.34911552980692306 1103 58-59 0.3385372597347569 1103 60-61 0.3300558882743374 1103 62-63 0.2921882675938434 1103 64-65 0.2964308874383548 1103 66-67 0.30510952607700403 1103 68-69 0.2702891487191508 1103 70-71 0.29657184818880467 1103 72-73 0.26863105288706635 1103 74-75 0.3245165803424541 1103 76-77 0.1655173732898021 1103 78-79 0.30094607710286425 1103 80-81 0.29674409503922305 1103 82-83 0.31708567086656814 1103 84-85 0.231979426734938 1103 86-87 0.1947365302027677 1103 88-89 0.22523941130732794 1103 90-91 0.2517189382581151 1103 92-93 0.21203279906047 1103 94-95 0.20705159035212617 1103 96-97 0.23630017773541923 1103 98-99 0.21072429827986738 1103 100 0.2563162947510591 1104 1 1.4314352915508515 1104 2 1.0519696149471898 1104 3 1.3976635069799421 1104 4 0.8661308876253742 1104 5 0.4943460803167383 1104 6 1.0084277515092097 1104 7 0.7410098705406085 1104 8 0.43120355089000384 1104 9 0.4098065150026926 1104 10-11 0.30548432212115273 1104 12-13 0.30664293175617274 1104 14-15 0.3711557505491214 1104 16-17 0.3614728426757452 1104 18-19 0.33989191622033843 1104 20-21 0.35259544339535154 1104 22-23 0.35591095424388186 1104 24-25 0.3482055725010511 1104 26-27 0.33127562180312964 1104 28-29 0.3257978800775234 1104 30-31 0.3349462567513797 1104 32-33 0.35842836055425664 1104 34-35 0.37565846341667 1104 36-37 0.3418791368475027 1104 38-39 0.38950679678077904 1104 40-41 0.3505630303433449 1104 42-43 0.37946557101219014 1104 44-45 0.3882887533561714 1104 46-47 0.39250632819909015 1104 48-49 0.33962761979757516 1104 50-51 0.321096373735017 1104 52-53 0.3435236125541792 1104 54-55 0.3758486081787922 1104 56-57 0.46711717706441647 1104 58-59 0.4300796605836652 1104 60-61 0.42162874736695954 1104 62-63 0.40959295069807666 1104 64-65 0.37330931080058605 1104 66-67 0.3926406251800927 1104 68-69 0.3591055533490106 1104 70-71 0.3456302576782093 1104 72-73 0.3296168127130983 1104 74-75 0.317961715724671 1104 76-77 0.233330633447423 1104 78-79 0.35189484832488205 1104 80-81 0.3754746691845625 1104 82-83 0.3927340400059123 1104 84-85 0.3576286751891047 1104 86-87 0.371694653272165 1104 88-89 0.35620874170281525 1104 90-91 0.37520994924799567 1104 92-93 0.38067968882026904 1104 94-95 0.36278537871087124 1104 96-97 0.3429139141308646 1104 98-99 0.38248650217711955 1104 100 0.39601670099417063 1105 1 1.3780187691340622 1105 2 1.0396037793034623 1105 3 1.3425926374943593 1105 4 0.8357284678785888 1105 5 0.43887680284701247 1105 6 0.9995624817918696 1105 7 0.7128465036646929 1105 8 0.36329799625222137 1105 9 0.3532399508671631 1105 10-11 0.26256007036501217 1105 12-13 0.24535669196738752 1105 14-15 0.315332525929918 1105 16-17 0.31655496110789016 1105 18-19 0.2962567213154159 1105 20-21 0.30414787283860534 1105 22-23 0.2998785934899004 1105 24-25 0.30276485994855307 1105 26-27 0.29431725839279466 1105 28-29 0.3079868921574018 1105 30-31 0.3242458974135616 1105 32-33 0.3602377687586653 1105 34-35 0.3325282553077855 1105 36-37 0.31988967217615283 1105 38-39 0.334896490665777 1105 40-41 0.38218289510758297 1105 42-43 0.3542688659547082 1105 44-45 0.30763653741470876 1105 46-47 0.3251460126569725 1105 48-49 0.343170888326199 1105 50-51 0.33323320895856057 1105 52-53 0.32735642230834117 1105 54-55 0.3930704573065924 1105 56-57 0.36452493270991226 1105 58-59 0.280736932752653 1105 60-61 0.241096772077384 1105 62-63 0.2620048452975041 1105 64-65 0.2379118163491185 1105 66-67 0.25492658469914886 1105 68-69 0.22873679065980923 1105 70-71 0.22703768681620318 1105 72-73 0.20678046734058597 1105 74-75 0.21584971198016234 1105 76-77 0.13986995620322773 1105 78-79 0.2537421034173235 1105 80-81 0.24851571770160774 1105 82-83 0.23667740816272698 1105 84-85 0.2262617415105268 1105 86-87 0.2561403080289395 1105 88-89 0.24746109399333704 1105 90-91 0.2251710736069512 1105 92-93 0.22210947668915892 1105 94-95 0.21147799449384408 1105 96-97 0.22617079802228446 1105 98-99 0.2380157241495482 1105 100 0.24585134484660998 1106 1 1.329213810535773 1106 2 0.9905340614110614 1106 3 1.3244746128716507 1106 4 0.7950536485552604 1106 5 0.40386712474111874 1106 6 0.9400402035359932 1106 7 0.6982937184692588 1106 8 0.33474077005002556 1106 9 0.30270780405798803 1106 10-11 0.2378985440251853 1106 12-13 0.20964807728731216 1106 14-15 0.23253872369858897 1106 16-17 0.23554001449276996 1106 18-19 0.19914937212007544 1106 20-21 0.22803240414981474 1106 22-23 0.24962075172008014 1106 24-25 0.2450975731736733 1106 26-27 0.21479943788600053 1106 28-29 0.22569428391852853 1106 30-31 0.2441070351623793 1106 32-33 0.26500514826781085 1106 34-35 0.27445015232406433 1106 36-37 0.24528161603466714 1106 38-39 0.26355474705462 1106 40-41 0.2962253481660113 1106 42-43 0.2819145903607705 1106 44-45 0.2802095952368546 1106 46-47 0.2909431677034888 1106 48-49 0.25650654644440607 1106 50-51 0.2703365718483539 1106 52-53 0.26112135437255546 1106 54-55 0.31168212435193965 1106 56-57 0.31334580161407644 1106 58-59 0.30428131763382993 1106 60-61 0.26814468213478193 1106 62-63 0.23591549446310722 1106 64-65 0.1963408503804729 1106 66-67 0.22249290644504782 1106 68-69 0.23686893506720708 1106 70-71 0.22439422758888838 1106 72-73 0.14215069309468475 1106 74-75 0.19832000124984717 1106 76-77 0.07957474285537813 1106 78-79 0.2546622561363918 1106 80-81 0.2563677031055889 1106 82-83 0.24528336499994197 1106 84-85 0.2549902900266332 1106 86-87 0.2421461717228226 1106 88-89 0.23662083232478892 1106 90-91 0.21676990032469945 1106 92-93 0.2409739021831392 1106 94-95 0.22161522385867372 1106 96-97 0.23612021404590067 1106 98-99 0.24216922341463842 1106 100 0.2578808699213866 1107 1 1.2781418773287676 1107 2 0.9021468093325069 1107 3 1.2639972726662343 1107 4 0.775236767351295 1107 5 0.38179228642829344 1107 6 0.9432289035827637 1107 7 0.6618635076252417 1107 8 0.296005476564865 1107 9 0.2826110893646927 1107 10-11 0.19887413827134282 1107 12-13 0.1313689168342762 1107 14-15 0.18237054154923982 1107 16-17 0.18227425510656303 1107 18-19 0.14039295146288566 1107 20-21 0.14255931102017172 1107 22-23 0.1869272123317529 1107 24-25 0.18310670473702118 1107 26-27 0.14233099505219116 1107 28-29 0.1449953552483052 1107 30-31 0.13559717396355353 1107 32-33 0.17295670319327172 1107 34-35 0.20741977163804393 1107 36-37 0.16583263535508053 1107 38-39 0.17787528826524124 1107 40-41 0.22219714389050438 1107 42-43 0.23217228845697946 1107 44-45 0.2192340522735563 1107 46-47 0.27439441686343 1107 48-49 0.24394992375325586 1107 50-51 0.20405832956370062 1107 52-53 0.20511759950970543 1107 54-55 0.23781975859893123 1107 56-57 0.22517826241508487 1107 58-59 0.21385593756712495 1107 60-61 0.22989888015849402 1107 62-63 0.2152660804228148 1107 64-65 0.18306097179641512 1107 66-67 0.1743312893910769 1107 68-69 0.18091655195769363 1107 70-71 0.17792521510503434 1107 72-73 0.18773069321571967 1107 74-75 0.18191180306195776 1107 76-77 0.03805182575758437 1107 78-79 0.16162501934184093 1107 80-81 0.1913153639865115 1107 82-83 0.2019246000389856 1107 84-85 0.23482499305116278 1107 86-87 0.2172526016126355 1107 88-89 0.23642089069836913 1107 90-91 0.24690691753629324 1107 92-93 0.23255729374246314 1107 94-95 0.1967479379252275 1107 96-97 0.20912220378680146 1107 98-99 0.21272031624048537 1107 100 0.22367430453199333 1108 1 1.2353211924048644 1108 2 0.8744120020018329 1108 3 1.226238653491155 1108 4 0.7487712049004074 1108 5 0.33944147266485203 1108 6 0.8715947758458995 1108 7 0.612549844694712 1108 8 0.27556806161916114 1108 9 0.21709086331233607 1108 10-11 0.12090691151296085 1108 12-13 0.11259186309394664 1108 14-15 0.12310313154902275 1108 16-17 0.14934513220237733 1108 18-19 0.08024548763891914 1108 20-21 0.11669757119455682 1108 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-0.840709329715768 2316 88-89 -0.7168914199687393 2316 90-91 -0.7932215911415703 2316 92-93 -0.8586966052616312 2316 94-95 -0.8730981206054231 2316 96-97 -0.9879428015666996 2316 98-99 -1.145936838083177 2316 100 -1.0596051627177872 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 60.0 7 876.0 8 1045.0 9 398.0 10 383.0 11 698.0 12 894.0 13 1298.0 14 2282.0 15 5170.0 16 10315.0 17 16292.0 18 23549.0 19 33910.0 20 49015.0 21 73745.0 22 114206.0 23 194539.0 24 346411.0 25 495356.0 26 512033.0 27 418266.0 28 346480.0 29 346113.0 30 392559.0 31 475154.0 32 586888.0 33 749097.0 34 1029015.0 35 1580691.0 36 2834768.0 37 5767027.0 38 8011721.0 39 2552586.0 40 5111.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 16.25872346958449 34.002154982612566 28.910811449286054 20.82831009851689 2 20.32063626961759 34.86657534926017 27.078672981706088 17.73411539941616 3 25.91027779510327 25.947032729924004 27.540367885692685 20.60232158928004 4 19.26299210496954 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19.765249306753024 24-25 19.79031067211502 30.189449549760617 26.528106028451415 23.49213374967295 26-27 19.827505582628216 26.69177238423458 33.65741632618822 19.823305706948986 28-29 19.8212492070793 26.781207935167778 33.63063225768951 19.766910600063405 30-31 19.789824217093095 26.697539733985348 33.633250120387764 19.87938592853379 32-33 19.937266365257337 26.83066649148386 33.4132493661981 19.8188177770607 34-35 19.93541823256906 26.936937272579815 33.325129520065246 19.80251497478588 36-37 19.873842030097556 26.87803535330124 33.38779786930331 19.86032474729789 38-39 19.8818368451906 26.881371699842315 33.38650983592617 19.850281619040917 40-41 19.89492490053579 26.90276495011234 33.377035077753526 19.825275071598345 42-43 19.88294398914815 26.947866990912694 33.33096427378491 19.83822474615425 44-45 19.916106146026664 26.992790968682932 33.265209051896555 19.825893833393852 46-47 19.926991963902598 27.069219808421668 33.20301057384359 19.80077765383214 48-49 19.970229867025616 27.071914537791532 33.11340927725244 19.844446317930416 50-51 19.956218650876973 27.145120626766918 33.043105985703804 19.8555547366523 52-53 20.054412497029958 27.287143434592803 32.7783525195114 19.880091548865835 54-55 20.40140595016287 27.519097247457136 32.00429177292523 20.075205029454768 56-57 20.87232213939585 28.12507519385602 30.689561537465348 20.313041129282787 58-59 20.937957550458073 28.72295264906602 29.94127060342408 20.397819197051827 60-61 20.933618612030767 29.06147558449363 29.564709253242544 20.440196550233065 62-63 20.894386043000985 29.249921049833755 29.38522685045332 20.470466056711942 64-65 20.894401973297338 29.36417350874133 29.306933971904307 20.434490546057024 66-67 20.853242839128114 29.183255642291407 29.541231323976362 20.422270194604113 68-69 20.798504763817892 28.96894565240228 29.82374169562793 20.4088078881519 70-71 20.736740325764245 28.838489363838367 30.03289310072368 20.391877209673712 72-73 20.684643763281542 28.80241488115353 30.0609178541768 20.452023501388126 74-75 20.753428882208862 28.90869650346424 29.88350592614415 20.45436868818275 76-77 20.820337832637744 29.043292166722456 29.655943690233226 20.480426310406568 78-79 20.91442856318452 29.265956477795108 29.240434613811527 20.579180345208847 80-81 21.003585976058222 29.45192215849045 28.886159672999955 20.65833219245137 82-83 21.036792625416965 29.640613757926854 28.51968339651872 20.802910220137466 84-85 21.032049740836413 29.730871732612705 28.319235589146707 20.917842937404178 86-87 21.030857679103438 29.788048742408048 28.112089654098487 21.069003924390024 88-89 21.045482748598268 29.88785079244832 27.934240877048445 21.132425581904972 90-91 21.13338687419146 29.8583905748605 27.764900492152822 21.243322058795222 92-93 21.117165818370083 29.887377672499593 27.67249588438206 21.322960624748262 94-95 21.15844657349026 29.900685607982098 27.536652159437935 21.404215659089708 96-97 21.15639597879822 29.886394112940412 27.424264905811274 21.532945002450095 98-99 21.136147645580103 29.907227539875166 27.317361294257065 21.63926352028767 100 21.08594329882022 29.896367984383822 27.23902170819728 21.778667008598678 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 26411.0 1 20104.0 2 11092.5 3 7743.5 4 7006.0 5 7007.0 6 7111.0 7 7103.0 8 7087.5 9 7371.5 10 8270.5 11 15964.5 12 127739.0 13 152218.5 14 71257.0 15 75974.0 16 87317.0 17 98438.0 18 108032.5 19 117336.0 20 130208.0 21 151823.5 22 182698.5 23 216528.0 24 250340.0 25 286626.0 26 324512.0 27 370443.5 28 435119.5 29 496894.5 30 540524.5 31 590540.5 32 642156.0 33 674351.0 34 700251.5 35 742607.0 36 786047.5 37 808440.5 38 829838.0 39 843443.0 40 844953.0 41 865539.5 42 902440.0 43 896747.0 44 864548.5 45 855159.0 46 846761.0 47 834058.5 48 815102.5 49 794126.5 50 780030.0 51 764213.0 52 746003.0 53 715594.0 54 667660.0 55 613617.5 56 560596.0 57 508760.0 58 451976.0 59 385883.0 60 319094.5 61 263322.5 62 207921.0 63 150115.0 64 104161.0 65 71764.5 66 48996.5 67 35773.5 68 26602.0 69 18616.0 70 12858.0 71 9593.0 72 7895.0 73 6381.5 74 5775.5 75 5922.5 76 4450.5 77 1950.0 78 715.0 79 423.5 80 291.5 81 218.5 82 161.5 83 117.5 84 84.5 85 61.0 86 44.0 87 33.5 88 20.0 89 9.5 90 8.0 91 8.0 92 6.5 93 4.5 94 4.0 95 4.0 96 1.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33200075128018436 2 0.006353336470957339 3 2.3571471384168503 4 8.191874912961329E-4 5 3.706730729846755E-5 6 0.008799778752656197 7 0.008073259529606233 8 3.298990349563612E-4 9 0.0 10-11 6.301442240739485E-5 12-13 5.5600960947701325E-6 14-15 0.0 16-17 3.150721120369742E-5 18-19 0.0 20-21 3.5213941933544176E-4 22-23 4.262740339323769E-5 24-25 5.022620138942353E-4 26-27 0.003565874962112578 28-29 0.00643117781628412 30-31 0.0058547811877929495 32-33 0.015653523872142847 34-35 0.009972959028652695 36-37 0.005098608118904212 38-39 0.005326572058789787 40-41 0.008497680198173686 42-43 0.01935840123662468 44-45 0.03465237222797239 46-47 0.033875812140069496 48-49 0.040738824086380764 50-51 0.04073141062492107 52-53 0.06319605221315733 54-55 0.033890639062988885 56-57 0.022240384379080533 58-59 0.016648781073106703 60-61 0.017775627214980116 62-63 0.025292877135109332 64-65 0.045681749514631415 66-67 0.04867122784825282 68-69 0.05158471820191237 70-71 0.0618060281894648 72-73 0.06960313627969744 74-75 0.060501258972558744 76-77 0.060701422431970464 78-79 0.05484108114808275 80-81 0.05249657396145468 82-83 0.04862489371412974 84-85 0.050811864844739325 86-87 0.059035246968904345 88-89 0.06563508103339649 90-91 0.061568797422754604 92-93 0.0661429031433855 94-95 0.0746016626688958 96-97 0.07354524441088947 98-99 0.047781612473089596 100 0.006775903774159869 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.6977951E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.0092699596874 #Duplication Level Percentage of deduplicated Percentage of total 1 75.51635537182119 29.458378930710623 2 11.937316205010925 9.313319808708444 3 4.1026751745697485 4.801270903250968 4 2.120230139722153 3.3083451958834873 5 1.303844774650162 2.543101639992797 6 0.8898585155570029 2.0827638635573886 7 0.6336842203966699 1.7303691175853417 8 0.48085265384855563 1.500616878784834 9 0.3764257935580053 1.3215685860624409 >10 2.2280988998043227 16.40027254985803 >50 0.217743851025289 5.846776972856652 >100 0.19018615091449118 14.302929716662133 >500 0.002058270308201741 0.49213373712535585 >1k 5.744372902639125E-4 0.41034194629730475 >5k 3.821660920074639E-5 0.09794196605418957 >10k+ 5.7324913800838955E-5 6.389868186609957 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1604483 5.947386441616712 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 34601 0.12825658998342757 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 31362 0.11625048914945393 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.2602884481478966E-4 0.0 0.0 0.0 3.706730729846755E-6 2 1.2973557554463644E-4 1.1120192189540265E-5 0.0 0.0 7.41346145969351E-6 3 1.408557677341767E-4 1.482692291938702E-5 0.0 0.0 7.41346145969351E-6 4 1.482692291938702E-4 1.8533653649233776E-5 0.0 0.0 1.482692291938702E-5 5 1.482692291938702E-4 2.965384583877404E-5 0.0 0.0 2.965384583877404E-5 6 1.5197595992371695E-4 3.706730729846755E-5 0.0 0.0 2.965384583877404E-5 7 1.5568269065356372E-4 3.706730729846755E-5 0.0 0.0 2.965384583877404E-5 8 1.5938942138341047E-4 4.448076875816106E-5 0.0 0.0 3.3360576568620795E-5 9 1.5938942138341047E-4 4.818749948800782E-5 0.0 0.0 3.3360576568620795E-5 10-11 1.6124278674833385E-4 5.74543263126247E-5 0.0 0.0 4.262740339323769E-5 12-13 1.6680288284310398E-4 6.857451850216496E-5 0.0 0.0 4.818749948800782E-5 14-15 1.7792307503264424E-4 7.784134532678186E-5 0.0 0.0 5.374759558277795E-5 16-17 1.9460336331695465E-4 7.784134532678186E-5 0.0 0.0 6.857451850216496E-5 18-19 2.298173052504988E-4 8.896153751632212E-5 0.0 1.8533653649233776E-6 7.41346145969351E-5 20-21 2.4464422816988584E-4 1.0193509507078576E-4 0.0 3.706730729846755E-6 7.784134532678186E-5 22-23 2.742980740086599E-4 1.1120192189540266E-4 0.0 3.706730729846755E-6 9.266826824616888E-5 24-25 3.63259611524982E-4 1.1861538335509616E-4 0.0 3.706730729846755E-6 9.822836434093902E-5 26-27 5.875168206807107E-4 1.3344230627448318E-4 0.0 3.706730729846755E-6 1.0008172970586239E-4 28-29 0.0012899422939866708 1.4270913309910008E-4 0.0 3.706730729846755E-6 1.0378846043570915E-4 30-31 0.0030339591023795694 1.575360560184871E-4 0.0 3.706730729846755E-6 1.0934855653047928E-4 32-33 0.006701769159562934 1.6865624820802737E-4 0.0 3.706730729846755E-6 1.2417547944986628E-4 34-35 0.014002175331996118 1.7792307503264424E-4 0.0 3.706730729846755E-6 1.334423062744832E-4 36-37 0.02629925452826273 1.9274999795203127E-4 0.0 3.706730729846755E-6 1.5197595992371695E-4 38-39 0.045203581250481184 2.0387019014157153E-4 0.0 3.706730729846755E-6 1.575360560184871E-4 40-41 0.07262968192061732 2.057235555064949E-4 0.0 3.706730729846755E-6 1.649495174781806E-4 42-43 0.11106477285839833 2.1869711306095855E-4 0.0 3.706730729846755E-6 1.7606970966772085E-4 44-45 0.16183215693437947 2.316706706154222E-4 0.0 3.706730729846755E-6 1.81629805762491E-4 46-47 0.22851994949505244 2.390841320751157E-4 0.0 3.706730729846755E-6 1.9645672868187804E-4 48-49 0.31367096782109216 2.4649759353480923E-4 0.0 3.706730729846755E-6 2.149903823311118E-4 50-51 0.41844541863094054 2.6317788181911964E-4 0.0 5.5600960947701325E-6 2.1869711306095855E-4 52-53 0.5418814053002023 2.7059134327881313E-4 0.0 7.41346145969351E-6 2.2240384379080533E-4 54-55 0.6877394061543072 2.7059134327881313E-4 0.0 7.41346145969351E-6 2.316706706154222E-4 56-57 0.8610716951780363 2.780048047385066E-4 0.0 1.1120192189540265E-5 2.390841320751157E-4 58-59 1.0648455103206318 2.983918237526638E-4 0.0 1.1120192189540265E-5 2.4835095889973256E-4 60-61 1.2997984909973335 3.0951201594220406E-4 0.0 1.1120192189540265E-5 2.576177857243495E-4 62-63 1.5599646540984524 3.18778842766821E-4 0.0 1.1120192189540265E-5 2.5947115108927287E-4 64-65 1.8489228481436562 3.317524003212846E-4 0.0 1.1120192189540265E-5 2.761514393735833E-4 66-67 2.1695050154105475 3.410192271459015E-4 0.0 1.1120192189540265E-5 2.9468509302281703E-4 68-69 2.5249860524989463 3.5399278470036515E-4 0.0 1.1120192189540265E-5 3.039519198474339E-4 70-71 2.914720988261859 3.6140624616005863E-4 0.0 1.482692291938702E-5 3.076586505772807E-4 72-73 3.3353014837931907 3.669663422548288E-4 1.8533653649233776E-6 1.482692291938702E-5 3.1692547740189755E-4 74-75 3.7890868732024905 3.743798037145223E-4 3.706730729846755E-6 1.482692291938702E-5 3.261923042265144E-4 76-77 4.274145949779507 3.8179326517421576E-4 3.706730729846755E-6 1.482692291938702E-5 3.3360576568620796E-4 78-79 4.792257944274567 3.892067266339093E-4 3.706730729846755E-6 1.482692291938702E-5 3.447259578757483E-4 80-81 5.343161532171216 3.966201880936028E-4 3.706730729846755E-6 1.6680288284310397E-5 3.502860539705184E-4 82-83 5.9236170308115685 4.0032691882344956E-4 3.706730729846755E-6 2.5947115108927287E-5 3.5769951543021186E-4 84-85 6.533822750289671 4.114471110129898E-4 3.706730729846755E-6 3.1507211203697416E-5 3.63259611524982E-4 86-87 7.178817991032751 4.1886057247268336E-4 3.706730729846755E-6 3.5213941933544173E-5 3.688197076197521E-4 88 7.681039230888958 4.225673032025301E-4 3.706730729846755E-6 3.706730729846755E-5 3.706730729846755E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 206830 0.0 86.74857 8 TGGTATC 208845 0.0 85.84572 7 GTGGTAT 207150 0.0 85.8203 6 AGTGGTA 208225 0.0 85.43134 5 GTATCAA 210060 0.0 85.33584 9 GCAGTGG 209450 0.0 84.94305 3 AAGCAGT 204140 0.0 84.89573 1 CAGTGGT 218320 0.0 81.407295 4 AGCAGTG 219710 0.0 80.70322 2 CAACGCA 203055 0.0 45.115368 12-13 ATCAACG 204080 0.0 44.933624 10-11 ACGCAGA 205435 0.0 44.63395 14-15 TCAACGC 202630 0.0 44.250988 12-13 AGAGTAC 207310 0.0 44.103874 18-19 AACGCAG 204155 0.0 43.969994 14-15 CGCAGAG 205995 0.0 43.528454 16-17 GAGTACT 204705 0.0 43.450066 20-21 TATCAAC 208170 0.0 43.02148 10-11 AGTACTT 212610 0.0 42.733135 20-21 GTACTTT 215020 0.0 41.343166 22-23 >>END_MODULE