FastQCFastQC Report
Thu 26 May 2016
SRR1660718_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660718_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22224690
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13033065.864225777727383No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC369250.16614404970328045Illumina Single End PCR Primer 1 (100% over 50bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT298260.13420209685714402No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3202850.044.665786
GGTATCA3205700.044.5484858
TGGTATC3198900.044.5345577
AGTGGTA3220250.044.4723175
GTATCAA3213650.044.4131859
AAGCAGT3256750.044.195841
GCAGTGG3273450.044.010333
CAGTGGT3287250.043.5061234
GAGTACT1613100.042.85787620-21
AGCAGTG3366850.042.8000872
AGTACTT1671150.042.4877220-21
GTACTTT1683350.041.01515622-23
AGAGTAC1758850.040.92901618-19
TACTTTT1791900.039.6677622-23
ACTTTTT1946600.035.93037824-25
CTTTTTT2597500.027.8312424-25
ATCAACG3218750.023.3284110-11
ACGCAGA3222400.023.32629214-15
CAACGCA3229350.023.23983812-13
GCAGAGT3256950.022.91468416-17
Produced by FastQC (version 0.11.5)