##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660718_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 22224690 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.83494950885704 34.0 31.0 34.0 30.0 34.0 2 31.99725759954357 34.0 31.0 34.0 30.0 34.0 3 32.03630345350149 34.0 31.0 34.0 30.0 34.0 4 35.44230371717221 37.0 35.0 37.0 33.0 37.0 5 35.43678233532166 37.0 35.0 37.0 33.0 37.0 6 35.48020746296124 37.0 35.0 37.0 33.0 37.0 7 35.39964759913411 37.0 35.0 37.0 33.0 37.0 8 35.40548106632758 37.0 35.0 37.0 33.0 37.0 9 37.08653056578067 39.0 37.0 39.0 33.0 39.0 10-11 37.00568595107514 39.0 37.0 39.0 33.0 39.0 12-13 37.08462340757059 39.0 37.0 39.0 33.0 39.0 14-15 38.47915610971401 40.0 38.0 41.0 34.0 41.0 16-17 38.4576882737172 40.0 38.0 41.0 34.0 41.0 18-19 38.415521341355046 40.0 38.0 41.0 34.0 41.0 20-21 38.303444412497996 40.0 38.0 41.0 33.5 41.0 22-23 38.11724357010154 40.0 38.0 41.0 33.5 41.0 24-25 38.13998546211444 40.0 38.0 41.0 33.0 41.0 26-27 38.14837927998096 40.0 38.0 41.0 33.0 41.0 28-29 38.08605382122315 40.0 38.0 41.0 33.0 41.0 30-31 37.98577651701778 40.0 38.0 41.0 33.0 41.0 32-33 37.85961493726121 40.0 38.0 41.0 32.5 41.0 34-35 37.74458588623733 40.0 38.0 41.0 32.0 41.0 36-37 37.56366531996622 40.0 37.5 41.0 32.0 41.0 38-39 37.13953245242116 40.0 35.5 41.0 31.0 41.0 40-41 37.012031753873735 40.0 35.0 41.0 31.0 41.0 42-43 36.953022944302035 40.0 35.0 41.0 31.0 41.0 44-45 36.89429368868587 40.0 35.0 41.0 30.5 41.0 46-47 36.854884882533796 40.0 35.0 41.0 30.0 41.0 48-49 36.73683911901583 39.5 35.0 41.0 30.0 41.0 50-51 35.39015601567446 38.0 34.0 39.5 28.0 40.0 52-53 35.45508607769108 38.5 34.5 39.5 28.0 40.5 54-55 35.858188483168945 39.0 35.0 40.5 26.5 41.0 56-57 35.56470841663033 39.0 35.0 41.0 24.5 41.0 58-59 35.36221470355717 39.0 35.0 41.0 23.0 41.0 60-61 35.14205964177678 39.0 35.0 41.0 23.0 41.0 62-63 34.88842685769745 38.5 34.5 41.0 22.5 41.0 64-65 34.59937069988378 37.5 34.0 40.0 22.0 41.0 66-67 34.28584805907305 37.0 34.0 40.0 22.0 41.0 68-69 33.9911680657863 36.5 34.0 39.5 22.0 41.0 70-71 33.68757613716997 36.0 34.0 39.0 22.0 41.0 72-73 33.339898104315516 35.5 33.0 39.0 21.0 41.0 74-75 32.96511415907264 35.0 33.0 37.5 20.0 40.0 76-77 32.58212110045179 35.0 33.0 37.0 20.0 39.0 78-79 32.22711828601434 35.0 32.5 36.5 19.0 39.0 80-81 31.872532282789997 35.0 32.0 36.0 18.0 39.0 82-83 31.59758091113982 35.0 32.0 36.0 18.0 37.0 84-85 31.332351722341233 35.0 32.0 35.0 17.0 37.0 86-87 31.129938640313995 35.0 32.0 35.0 17.0 36.5 88-89 30.935575546835523 35.0 31.0 35.0 15.5 36.0 90-91 30.76427709452865 35.0 31.0 35.0 15.0 36.0 92-93 30.623664357073146 35.0 31.0 35.0 15.0 36.0 94-95 30.464937958639695 35.0 31.0 35.0 12.5 35.0 96-97 30.357105610921906 35.0 31.0 35.0 11.5 35.0 98-99 30.242665791963802 35.0 30.5 35.0 10.0 35.0 100 30.160908341128717 35.0 30.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5033718398950846 1101 2 0.509968090345545 1101 3 0.4857115290066858 1101 4 0.42018673040916354 1101 5 0.40692129818896916 1101 6 0.35773809708931026 1101 7 0.4063802324274022 1101 8 0.44522603573366837 1101 9 0.500974279824483 1101 10-11 0.48911594140732717 1101 12-13 0.46380261051457694 1101 14-15 0.5556424443626611 1101 16-17 0.5404661760804856 1101 18-19 0.5484316138132499 1101 20-21 0.581558821557131 1101 22-23 0.5983424869668568 1101 24-25 0.5745504256575416 1101 26-27 0.575768458039235 1101 28-29 0.5450238612684473 1101 30-31 0.5279460556723663 1101 32-33 0.5213164800910164 1101 34-35 0.5369278675572886 1101 36-37 0.49948106148480065 1101 38-39 0.4784985949735088 1101 40-41 0.5317196544098266 1101 42-43 0.5337472658761371 1101 44-45 0.5324546517285427 1101 46-47 0.5436317456572723 1101 48-49 0.5755981292143844 1101 50-51 0.4953707477875966 1101 52-53 0.5474805910036409 1101 54-55 0.6133233888380261 1101 56-57 0.6358399874298755 1101 58-59 0.5993110729278186 1101 60-61 0.6034584625537747 1101 62-63 0.5837531404468521 1101 64-65 0.5450929308150236 1101 66-67 0.5427135866245365 1101 68-69 0.5527332993204084 1101 70-71 0.5094936697894497 1101 72-73 0.5167177960645617 1101 74-75 0.4908517169162039 1101 76-77 0.49317065389102055 1101 78-79 0.464325029348565 1101 80-81 0.41542426997402515 1101 82-83 0.40191429537611256 1101 84-85 0.35401479679384096 1101 86-87 0.36020838187990023 1101 88-89 0.3794605459673477 1101 90-91 0.3403511684471425 1101 92-93 0.38006484665335805 1101 94-95 0.3970304838117542 1101 96-97 0.35370332165683394 1101 98-99 0.2506419441063201 1101 100 0.23858944494016399 1102 1 0.45607454259778635 1102 2 0.49205948092006224 1102 3 0.4661132414063971 1102 4 0.3863820832388356 1102 5 0.4119021057361465 1102 6 0.38935627997776123 1102 7 0.3902032375150881 1102 8 0.4344558945556045 1102 9 0.4815585235714295 1102 10-11 0.45816940355592806 1102 12-13 0.4881959376956999 1102 14-15 0.5688984603760474 1102 16-17 0.5299458134152175 1102 18-19 0.513326206675444 1102 20-21 0.5137043009269675 1102 22-23 0.5537833461583688 1102 24-25 0.503787942460022 1102 26-27 0.4892943661740219 1102 28-29 0.498176139680659 1102 30-31 0.46663126368033403 1102 32-33 0.5108295663708091 1102 34-35 0.4572607000451612 1102 36-37 0.4824208421137399 1102 38-39 0.4966796625306529 1102 40-41 0.4686532809917239 1102 42-43 0.4399765421997657 1102 44-45 0.47468713607727864 1102 46-47 0.5285598466084949 1102 48-49 0.5122400409451231 1102 50-51 0.3976560756482641 1102 52-53 0.4979450318760641 1102 54-55 0.5652479058506259 1102 56-57 0.6118866192410266 1102 58-59 0.49187143145022105 1102 60-61 0.49221546762208845 1102 62-63 0.5091275427667128 1102 64-65 0.508723472558934 1102 66-67 0.5284011870295799 1102 68-69 0.48282261743415233 1102 70-71 0.48629367709163773 1102 72-73 0.4653280849553951 1102 74-75 0.47729048073447444 1102 76-77 0.45470352881353193 1102 78-79 0.39665612533206485 1102 80-81 0.4098646315178911 1102 82-83 0.40253145344383157 1102 84-85 0.37646519781793586 1102 86-87 0.3405657130131381 1102 88-89 0.3593507910488043 1102 90-91 0.394730188374659 1102 92-93 0.34259607886359333 1102 94-95 0.3792024173037518 1102 96-97 0.3624822900964908 1102 98-99 0.28538939048369016 1102 100 0.267352021650364 1103 1 0.3759610889718168 1103 2 0.40852848634321504 1103 3 0.396330974045938 1103 4 0.34408927750737917 1103 5 0.323447760631403 1103 6 0.3423207307590701 1103 7 0.3511428602651492 1103 8 0.39767272992169467 1103 9 0.4433752319883979 1103 10-11 0.39843869346223926 1103 12-13 0.41572050701137186 1103 14-15 0.47953149520162697 1103 16-17 0.4925459032465369 1103 18-19 0.46789401606322656 1103 20-21 0.47523306263068577 1103 22-23 0.5150177928986608 1103 24-25 0.5078034707665964 1103 26-27 0.4593005035209785 1103 28-29 0.45507641816813305 1103 30-31 0.454822621713582 1103 32-33 0.44076002377926216 1103 34-35 0.467886277086599 1103 36-37 0.4502015467835889 1103 38-39 0.42579967346151193 1103 40-41 0.4438473544988284 1103 42-43 0.4508593618911263 1103 44-45 0.4668524013534494 1103 46-47 0.4419385070093895 1103 48-49 0.44275724379823345 1103 50-51 0.3528933635811953 1103 52-53 0.398170309809025 1103 54-55 0.473707731982671 1103 56-57 0.5043167690673229 1103 58-59 0.46629662539159256 1103 60-61 0.4755986018270306 1103 62-63 0.4600073988390605 1103 64-65 0.42700772170151424 1103 66-67 0.4116827498103035 1103 68-69 0.4090610493127613 1103 70-71 0.43005284390618215 1103 72-73 0.4276195312603974 1103 74-75 0.3765646583377773 1103 76-77 0.38964212761827355 1103 78-79 0.3568981430987037 1103 80-81 0.32052274945129966 1103 82-83 0.3283795592403109 1103 84-85 0.2955600883845406 1103 86-87 0.3037655663787646 1103 88-89 0.3346221716473181 1103 90-91 0.36264746385181823 1103 92-93 0.31822102731109325 1103 94-95 0.3137153830611048 1103 96-97 0.3086201932977026 1103 98-99 0.32167527925574646 1103 100 0.26884104417334953 1104 1 0.34709384646420816 1104 2 0.36698895092455786 1104 3 0.35768586212449094 1104 4 0.31866488111584346 1104 5 0.3143868947110775 1104 6 0.3069306525550246 1104 7 0.271473650442573 1104 8 0.33924457756808124 1104 9 0.3884231916416354 1104 10-11 0.3514926671351759 1104 12-13 0.35592583668901057 1104 14-15 0.39721180919487864 1104 16-17 0.4068816474277952 1104 18-19 0.39133850842226536 1104 20-21 0.36029457022125655 1104 22-23 0.38177167327599193 1104 24-25 0.3918149177454424 1104 26-27 0.3429923661694829 1104 28-29 0.31153541664060214 1104 30-31 0.3051131816052717 1104 32-33 0.30830893393027736 1104 34-35 0.3078375979997716 1104 36-37 0.3207818597312766 1104 38-39 0.336935017892543 1104 40-41 0.3705315009718291 1104 42-43 0.32021561156361855 1104 44-45 0.37242547544727245 1104 46-47 0.3755923732308375 1104 48-49 0.35053374665581316 1104 50-51 0.32837401026603175 1104 52-53 0.34186527533400834 1104 54-55 0.5225810204484986 1104 56-57 0.5073838448860712 1104 58-59 0.46809086480937623 1104 60-61 0.44927536780575394 1104 62-63 0.4468176569370357 1104 64-65 0.39912294102420987 1104 66-67 0.4125122554219942 1104 68-69 0.39744726367032257 1104 70-71 0.366103230131543 1104 72-73 0.34796241580479403 1104 74-75 0.3412205683387768 1104 76-77 0.328159009128683 1104 78-79 0.32588856395163646 1104 80-81 0.31300846666247395 1104 82-83 0.3347328849043123 1104 84-85 0.33857598404832956 1104 86-87 0.3342064703304395 1104 88-89 0.3148071084558097 1104 90-91 0.30057430464306023 1104 92-93 0.3432206428848197 1104 94-95 0.38384567528347446 1104 96-97 0.32969312426038044 1104 98-99 0.3478447759532344 1104 100 0.30871490600101126 1105 1 0.2713935331183528 1105 2 0.2984838802185763 1105 3 0.28697105336322437 1105 4 0.26865639537012953 1105 5 0.29968819209006625 1105 6 0.2655490327108154 1105 7 0.2809676588583443 1105 8 0.29670311812719774 1105 9 0.34386611441601644 1105 10-11 0.3446280432689619 1105 12-13 0.31642852865378757 1105 14-15 0.36082939895915445 1105 16-17 0.3176248823862835 1105 18-19 0.35023269919845035 1105 20-21 0.3518696492083322 1105 22-23 0.3797631038574991 1105 24-25 0.37343406356733055 1105 26-27 0.369539210036713 1105 28-29 0.346508514285361 1105 30-31 0.383068162459999 1105 32-33 0.41783334657613125 1105 34-35 0.3801053259330516 1105 36-37 0.3627298281040794 1105 38-39 0.37230480886326234 1105 40-41 0.38348330268137687 1105 42-43 0.337069748053203 1105 44-45 0.3491608199997813 1105 46-47 0.39416155803538544 1105 48-49 0.3985933292782633 1105 50-51 0.31117577688326037 1105 52-53 0.3798149785363947 1105 54-55 0.49059933924552723 1105 56-57 0.5219736095251903 1105 58-59 0.5092522202227627 1105 60-61 0.4561966962680657 1105 62-63 0.423367663612332 1105 64-65 0.43560444159219713 1105 66-67 0.38227617819287474 1105 68-69 0.3736928411130904 1105 70-71 0.39611342286688256 1105 72-73 0.35330607579960116 1105 74-75 0.3474662114166236 1105 76-77 0.37233416330852975 1105 78-79 0.36848146141877436 1105 80-81 0.3862365900288225 1105 82-83 0.35975946710943774 1105 84-85 0.38153612981972174 1105 86-87 0.33438858138869065 1105 88-89 0.3245324184086087 1105 90-91 0.31668946137149945 1105 92-93 0.2779159508253848 1105 94-95 0.3562120526379928 1105 96-97 0.3278251113505064 1105 98-99 0.2931919542555299 1105 100 0.32219897759135563 1106 1 0.21901007833836061 1106 2 0.23948095300664818 1106 3 0.2208639606741798 1106 4 0.2089419633088454 1106 5 0.2021636294727145 1106 6 0.19299435713771373 1106 7 0.18971007601441414 1106 8 0.2439124606858556 1106 9 0.2484586772034234 1106 10-11 0.20090281363776086 1106 12-13 0.2033265411115721 1106 14-15 0.24618220070337316 1106 16-17 0.24314773793179256 1106 18-19 0.20671141497194867 1106 20-21 0.2551050299122508 1106 22-23 0.2551200873521964 1106 24-25 0.23838727991269337 1106 26-27 0.24385877238993459 1106 28-29 0.2377518620722725 1106 30-31 0.23102598687695064 1106 32-33 0.24092827421106477 1106 34-35 0.1971545670089867 1106 36-37 0.1955869845965097 1106 38-39 0.23256994970853384 1106 40-41 0.2533657770068132 1106 42-43 0.22522567165514573 1106 44-45 0.2347853698381499 1106 46-47 0.2635862106026181 1106 48-49 0.2414435123287717 1106 50-51 0.1955490267139126 1106 52-53 0.22678615395464163 1106 54-55 0.3095549525713608 1106 56-57 0.2890032669805578 1106 58-59 0.2625700835116902 1106 60-61 0.24953828136022338 1106 62-63 0.26689002876746315 1106 64-65 0.1683582241743622 1106 66-67 0.1887416354986513 1106 68-69 0.17437630214271138 1106 70-71 0.19657226277138307 1106 72-73 0.1405470476079742 1106 74-75 0.15760724051911978 1106 76-77 0.18120269945474377 1106 78-79 0.17991406729711912 1106 80-81 0.1561684280277973 1106 82-83 0.19907667805189178 1106 84-85 0.19075279067183715 1106 86-87 0.18506074651304516 1106 88-89 0.21068023109567946 1106 90-91 0.18488317909115537 1106 92-93 0.1527344953495806 1106 94-95 0.21733462816624183 1106 96-97 0.15600658682728863 1106 98-99 0.1696215225858957 1106 100 0.18269759053069023 1107 1 0.17910988891778956 1107 2 0.20491558088090045 1107 3 0.21434854224646926 1107 4 0.21058150836702794 1107 5 0.22238182025379416 1107 6 0.2022184056766747 1107 7 0.20893258015643568 1107 8 0.22652263054843758 1107 9 0.2379036057532673 1107 10-11 0.2496213786807857 1107 12-13 0.22685554295095045 1107 14-15 0.24586238657351345 1107 16-17 0.24610560386517477 1107 18-19 0.22800041607229815 1107 20-21 0.22624138204381694 1107 22-23 0.24504095191322506 1107 24-25 0.22804136809498488 1107 26-27 0.1796956358662385 1107 28-29 0.21006103377673924 1107 30-31 0.1802944522576837 1107 32-33 0.15718158744454058 1107 34-35 0.19390066556901786 1107 36-37 0.22042350180704773 1107 38-39 0.23073399118374027 1107 40-41 0.2962782612522332 1107 42-43 0.25004317626403605 1107 44-45 0.26558409182902665 1107 46-47 0.235618426647612 1107 48-49 0.24417374609311793 1107 50-51 0.17488804877876873 1107 52-53 0.21603818996786828 1107 54-55 0.3391913505338522 1107 56-57 0.36685589803242635 1107 58-59 0.3555226562400975 1107 60-61 0.3379449373548482 1107 62-63 0.3376831216413194 1107 64-65 0.33136105130147087 1107 66-67 0.2994599478122453 1107 68-69 0.30158802800671225 1107 70-71 0.2823398950555287 1107 72-73 0.27996906867839755 1107 74-75 0.2343237249373331 1107 76-77 0.24542697890829146 1107 78-79 0.2701227932594179 1107 80-81 0.2318407134648055 1107 82-83 0.2951544843466216 1107 84-85 0.2463920162678832 1107 86-87 0.25766397880915193 1107 88-89 0.27446744136025814 1107 90-91 0.25881726408339745 1107 92-93 0.22719973117572323 1107 94-95 0.295175184456113 1107 96-97 0.2975452069227771 1107 98-99 0.2797878547859298 1107 100 0.28130089759953947 1108 1 0.06918763394622474 1108 2 0.11794021575996538 1108 3 0.11554620499759238 1108 4 0.11921634818738625 1108 5 0.09659097127915572 1108 6 0.07136079574548404 1108 7 0.09816256460722883 1108 8 0.13374915626562967 1108 9 0.14381171581285201 1108 10-11 0.09659595001318166 1108 12-13 0.11131367916249246 1108 14-15 0.12112234063812366 1108 16-17 0.08852591900350149 1108 18-19 0.09186044261629434 1108 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-1.066004725515974 2316 66-67 -1.0657693669552444 2316 68-69 -0.9642427450764401 2316 70-71 -1.1372584488842747 2316 72-73 -0.8952115368467446 2316 74-75 -0.998135470732386 2316 76-77 -0.9733130676908175 2316 78-79 -0.7958638001780187 2316 80-81 -0.8729299030321016 2316 82-83 -0.8529141775182119 2316 84-85 -0.8229439761963739 2316 86-87 -0.9175244741821409 2316 88-89 -0.991233598253995 2316 90-91 -0.9155170447212342 2316 92-93 -0.7841092773218641 2316 94-95 -0.8324796807180874 2316 96-97 -0.8738351502219928 2316 98-99 -0.8443515477696231 2316 100 -0.8885894629426545 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5798.0 3 1386.0 4 1034.0 5 885.0 6 1910.0 7 13572.0 8 28961.0 9 35617.0 10 36774.0 11 34393.0 12 33008.0 13 34017.0 14 38260.0 15 46216.0 16 59829.0 17 74964.0 18 82077.0 19 91278.0 20 105331.0 21 129495.0 22 169003.0 23 258772.0 24 369954.0 25 419486.0 26 413494.0 27 358239.0 28 311761.0 29 308020.0 30 343902.0 31 412574.0 32 513586.0 33 665332.0 34 918906.0 35 1405290.0 36 2473879.0 37 4675760.0 38 5705589.0 39 1643057.0 40 3281.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 14.26865414905811 32.70221850880881 28.459600068624745 24.569527273508328 2 20.93276320296272 34.11190132488053 26.635578078444848 18.319757393711903 3 25.958183310314098 25.320662728729022 26.335949589111703 22.38520437184518 4 18.970645243911854 27.871171398344842 24.43464502120861 28.723538336534695 5 22.288366136418837 32.57094717754673 25.701824706836085 19.43886197919835 6 29.068929690969625 26.860478351088794 25.37613517706295 18.694456780878635 7 21.304813940021894 25.487323959391244 32.25639138248902 20.951470718097845 8 27.860332618708462 26.577866994935697 25.198386868206217 20.363413518149624 9 26.790423647909282 25.1012364515798 27.11242060904833 20.99591929146259 10-11 20.71243547084012 30.01324607382297 28.82223188070046 20.452086574636454 12-13 20.469089012057093 26.414239036592374 28.91401007364907 24.20266187770146 14-15 20.592565338108376 33.108009028132116 25.624195253586546 20.675230380172962 16-17 24.185945467807016 26.102785129823737 25.70119065770174 24.01007874466751 18-19 20.555725374127977 29.50739294697737 25.98572926574522 23.951152413149433 20-21 23.8563120070091 29.914719004603775 25.82367008341673 20.405298904970394 22-23 24.120624345387984 26.363471037099995 29.095441486377766 20.420463131134255 24-25 20.57921588035886 29.77242685010475 25.510583497821056 24.13777377171534 26-27 20.635262164879002 26.399477358194595 32.39191616788706 20.57334430903934 28-29 20.84445675492584 26.43144443102597 32.40736253325996 20.316736280788223 30-31 20.63108982021479 26.294713641523664 32.57086665191114 20.503329886350404 32-33 20.676827698684804 26.4233179000966 32.3895028059571 20.510351595261497 34-35 20.741918429057424 26.765134645429377 32.042911522833826 20.45003540267937 36-37 20.61163144929288 26.54548475641481 32.2858813077629 20.557002486529413 38-39 20.56326050339666 26.43565808584804 32.31983900295927 20.681242407796024 40-41 20.732069352521787 26.442201478727135 32.263254909014435 20.562474259736643 42-43 20.790925611761317 26.473974387572547 32.34918568352768 20.38591431713846 44-45 20.759442524679166 26.642100342614093 32.13696524962416 20.461491883082576 46-47 20.53090249196786 26.706717141566642 32.116101572918716 20.646278793546777 48-49 20.891362733120186 26.64509063253361 31.931147716774856 20.532398917571353 50-51 20.923228365067523 26.719039749037798 31.852903663812214 20.50482822208246 52-53 20.85713351217425 26.87411150644859 31.805955990979783 20.462798990397378 54-55 21.187017810930676 27.06861865399241 31.107158708991488 20.63720482608543 56-57 21.615787698748466 27.79111092323156 29.8389267491695 20.754174628850475 58-59 21.676325624684697 28.207046126289036 29.189725729470723 20.92690251955554 60-61 21.903954745287376 28.657715767155906 28.561515829029272 20.87681365852745 62-63 22.094528525624575 28.81901357153983 28.331435766810458 20.75502213602513 64-65 22.064193660401966 28.82201771634888 28.317714012296015 20.79607461095314 66-67 22.11827397133902 28.745161997106973 28.394115194776138 20.742448836777868 68-69 22.285861230807104 28.46655090125659 28.671684218327297 20.57590364960901 70-71 22.27763145249636 28.383833133364995 28.74357353568957 20.594961878449073 72-73 22.49798885201487 28.15139635247752 28.834246852345462 20.516367943162145 74-75 22.852608407554527 28.210030044909225 28.69708331499588 20.24027823254037 76-77 23.034981893962136 28.344310868023864 28.41641309433881 20.204294143675188 78-79 23.25389004122521 28.446060551920443 28.074291195345552 20.225758211508793 80-81 23.60947550662598 28.68579641502916 27.620347586007643 20.08438049233722 82-83 23.827189262916846 28.71574878685929 27.352257596063488 20.104804354160372 84-85 23.915168726218944 28.822240688365643 27.17497180141261 20.087618784002807 86-87 24.12008089533294 28.858525247372175 27.019394662826173 20.001999194468713 88-89 24.389008720861316 28.87476670963717 26.80923758355615 19.926986985945362 90-91 24.46633085086118 28.90302233999798 26.628900165300767 20.00174664384007 92-93 24.598730920992317 28.896867225182227 26.567938657548957 19.9364631962765 94-95 24.924277775440135 28.844525129566463 26.4853093019606 19.7458877930328 96-97 25.07280914113727 28.847645753531975 26.40300655752239 19.676538547808367 98-99 25.23508670277132 28.837362994419856 26.312194817078687 19.61535548573013 100 25.316714898847493 28.923045752472675 26.262250242286513 19.497989106393323 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 24428.0 1 18356.5 2 10524.5 3 8204.0 4 7643.5 5 7768.5 6 7969.0 7 7926.5 8 7923.5 9 8134.5 10 8583.5 11 10868.0 12 89852.0 13 110387.0 14 53601.5 15 56778.5 16 64000.5 17 71023.5 18 77256.0 19 83743.5 20 91586.5 21 104186.0 22 122530.0 23 143779.5 24 166379.0 25 192139.0 26 218370.5 27 248163.5 28 285537.5 29 322201.0 30 352839.5 31 384238.5 32 418460.5 33 445441.5 34 470786.0 35 503474.5 36 536700.5 37 562995.0 38 590530.5 39 613770.0 40 628452.5 41 655823.5 42 691231.5 43 699123.0 44 692761.0 45 703403.0 46 719511.0 47 796526.0 48 890266.5 49 905779.5 50 839716.5 51 750210.5 52 701783.0 53 665034.0 54 626136.5 55 585620.5 56 542718.0 57 494594.0 58 439203.5 59 377179.0 60 310781.0 61 251975.5 62 198734.5 63 147841.0 64 107401.0 65 77257.0 66 54626.0 67 39637.0 68 29108.0 69 21138.0 70 15968.0 71 12619.0 72 10364.0 73 8430.5 74 7157.5 75 6587.0 76 5250.0 77 3377.5 78 2261.5 79 1779.0 80 1455.0 81 1230.0 82 1103.0 83 1000.0 84 848.5 85 708.5 86 611.0 87 540.5 88 488.5 89 440.5 90 382.0 91 313.5 92 275.0 93 255.0 94 213.5 95 172.0 96 140.5 97 106.0 98 78.5 99 53.5 100 29.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28611422701509 2 0.25389330514846326 3 0.13266776724444748 4 0.11133563617760248 5 0.14929342096560175 6 0.0632539756460045 7 0.08077952943325643 8 0.07964565534997338 9 0.07661299212722426 10-11 0.07299314411134644 12-13 0.07134407724022247 14-15 0.07647125786681389 16-17 0.07632952360640351 18-19 0.08091901394350157 20-21 0.07907196905783613 22-23 0.09313065784044683 24-25 0.057589104729919745 26-27 0.05124255951376599 28-29 0.0556250728356616 30-31 0.05194448156532217 32-33 0.05215820783102036 34-35 0.05256316286076431 36-37 0.05038765445097322 38-39 0.050007446673046956 40-41 0.05036515694932078 42-43 0.03166748332597665 44-45 0.033384042702057935 46-47 0.042997225158146184 48-49 0.050884849237492175 50-51 0.056603714157542805 52-53 0.056623961909030004 54-55 0.05542934457128535 56-57 0.05344056542520953 58-59 0.05311210190108389 60-61 0.05209521482639353 62-63 0.05412898897577424 64-65 0.057490115722649004 66-67 0.061604908774880555 68-69 0.06414487671144119 70-71 0.06340020940674539 72-73 0.05792431750454112 74-75 0.061969368301650105 76-77 0.06550147606108342 78-79 0.0684868945303624 80-81 0.08053430666524482 82-83 0.0581425432705698 84-85 0.06061276895200787 86-87 0.07084463270353827 88-89 0.07220123205318049 90-91 0.07372206316488554 92-93 0.07547461854361073 94-95 0.07073889444577179 96-97 0.07095037096130474 98-99 0.07328786138299342 100 0.06486029726398883 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.222469E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.968503795420546 #Duplication Level Percentage of deduplicated Percentage of total 1 73.42393776656954 31.549167485975648 2 13.940277997969739 11.979857761299604 3 4.773286515858331 6.1530293931986515 4 2.300577176657095 3.9540943658739303 5 1.287359408417913 2.765795381333772 6 0.8497977654245328 2.1908723105390364 7 0.5960899597561525 1.7929165588736022 8 0.4110615037500647 1.4130158227228746 9 0.3116039621403926 1.2050240426907786 >10 1.830759817308132 14.51372798656802 >50 0.15404838022719272 4.668751241698614 >100 0.11893657220351068 8.389362653851375 >500 0.0010599882758705937 0.28567796637909165 >1k 7.11656793330117E-4 0.6120574352434531 >5k 1.4641278885917016E-4 0.4697233604754251 >10k+ 3.4511585945371377E-4 8.056926233275965 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1303306 5.864225777727383 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 36925 0.16614404970328045 Illumina Single End PCR Primer 1 (100% over 50bp) AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 29826 0.13420209685714402 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.1955392853623605 1.7998001321953197E-5 0.0 4.499500330488299E-6 1.3498500991464899E-5 2 0.20284197439874302 3.5996002643906394E-5 0.0 4.499500330488299E-6 1.3498500991464899E-5 3 0.211116555506511 3.5996002643906394E-5 0.0 4.499500330488299E-6 1.7998001321953197E-5 4 0.21209744657855745 5.3994003965859594E-5 0.0 4.499500330488299E-6 1.7998001321953197E-5 5 0.21217393808417576 5.3994003965859594E-5 0.0 4.499500330488299E-6 1.7998001321953197E-5 6 0.21228642559243796 6.299300462683618E-5 0.0 4.499500330488299E-6 1.7998001321953197E-5 7 0.21244390810400504 7.199200528781279E-5 0.0 8.999000660976599E-6 2.2497501652441495E-5 8 0.21381175620447349 8.549050627927768E-5 0.0 8.999000660976599E-6 2.2497501652441495E-5 9 0.2154270768231188 8.549050627927768E-5 0.0 1.3498500991464899E-5 2.2497501652441495E-5 10-11 0.2158455303538542 1.0348850760123087E-4 0.0 1.7998001321953197E-5 2.4747251817685646E-5 12-13 0.2160232606169085 1.3723476007989314E-4 0.0 1.7998001321953197E-5 2.9246752148173945E-5 14-15 0.21620774013045851 1.50733261071358E-4 0.0 1.7998001321953197E-5 3.149650231341809E-5 16-17 0.21637422164268658 1.5748251156709047E-4 0.0 1.7998001321953197E-5 3.8245752809150545E-5 18-19 0.2165024574021055 1.6873126239331122E-4 0.0 1.7998001321953197E-5 4.274525313963885E-5 20-21 0.2166036961595415 1.8222976338477613E-4 0.0 1.7998001321953197E-5 4.724475347012714E-5 22-23 0.216792675173422 2.0922676536770592E-4 0.0 1.7998001321953197E-5 5.624375413110374E-5 24-25 0.2171841317021745 2.2722476668965913E-4 0.0 1.7998001321953197E-5 5.849350429634789E-5 26-27 0.21832925453628374 2.3397401718539156E-4 0.0 1.7998001321953197E-5 5.849350429634789E-5 28-29 0.22158239327522677 2.542217686725889E-4 0.0 2.0247751487197345E-5 5.849350429634789E-5 30-31 0.2289525748165666 2.632207693335655E-4 0.0 2.2497501652441495E-5 5.849350429634789E-5 32-33 0.24383692190982192 2.6997001982929794E-4 0.0 2.2497501652441495E-5 6.299300462683618E-5 34-35 0.27107014766010235 2.7446952015978623E-4 0.0 2.6997001982929797E-5 6.974225512256863E-5 36-37 0.3147715446199699 2.812187706555187E-4 0.0 2.6997001982929797E-5 7.424175545305695E-5 38-39 0.37970158413908134 2.8796802115125115E-4 0.0 2.6997001982929797E-5 7.649150561830109E-5 40-41 0.46761732109649223 2.9471727164698356E-4 0.0 2.6997001982929797E-5 7.649150561830109E-5 42-43 0.5800778323567168 3.1046552280369265E-4 2.2497501652441496E-6 3.374625247866224E-5 7.649150561830109E-5 44-45 0.7207277131874505 3.2846352412564585E-4 4.499500330488299E-6 4.0495502974394696E-5 7.874125578354523E-5 46-47 0.8924781403025195 3.6220977660430804E-4 4.499500330488299E-6 4.0495502974394696E-5 8.549050627927768E-5 48-49 1.0992144322373 3.8695702842199376E-4 4.499500330488299E-6 4.0495502974394696E-5 8.999000660976598E-5 50-51 1.3378476820149123 3.892067785872379E-4 4.499500330488299E-6 4.0495502974394696E-5 9.223975677501013E-5 52-53 1.602749014721915 4.027052795787028E-4 4.499500330488299E-6 4.0495502974394696E-5 9.898900727074259E-5 54-55 1.8998780185460404 4.162037805701677E-4 4.499500330488299E-6 4.0495502974394696E-5 1.0573825776647504E-4 56-57 2.239545298494602 4.387012822226092E-4 4.499500330488299E-6 4.0495502974394696E-5 1.0798800793171919E-4 58-59 2.6217778515695835 4.499500330488299E-4 4.499500330488299E-6 4.0495502974394696E-5 1.1248750826220748E-4 60-61 3.045547541945467 4.634485340402948E-4 4.499500330488299E-6 4.0495502974394696E-5 1.1248750826220748E-4 62-63 3.500519017363122 4.7919678519700386E-4 4.499500330488299E-6 4.499500330488299E-5 1.1248750826220748E-4 64-65 3.987781156902526 4.859460356927363E-4 4.499500330488299E-6 4.724475347012714E-5 1.1248750826220748E-4 66-67 4.509369984463225 4.97194786518957E-4 4.499500330488299E-6 4.949450363537129E-5 1.1473725842745162E-4 68-69 5.067101048428572 5.129430376756662E-4 4.499500330488299E-6 4.949450363537129E-5 1.2373625908842822E-4 70-71 5.6624749321587835 5.151927878409103E-4 4.499500330488299E-6 4.949450363537129E-5 1.2598600925367237E-4 72-73 6.288695140404657 5.219420383366427E-4 4.499500330488299E-6 5.3994003965859594E-5 1.2598600925367237E-4 74-75 6.940454512526384 5.4218978982384E-4 4.499500330488299E-6 5.3994003965859594E-5 1.282357594189165E-4 76-77 7.622401032365357 5.579380409805492E-4 4.499500330488299E-6 5.624375413110374E-5 1.3498500991464897E-4 78-79 8.330606636133059 5.669370416415257E-4 4.499500330488299E-6 5.849350429634789E-5 1.394845102451373E-4 80-81 9.062472862388631 5.759360423025023E-4 4.499500330488299E-6 6.074325446159204E-5 1.394845102451373E-4 82-83 9.81963078000188 5.804355426329906E-4 4.499500330488299E-6 6.524275479208034E-5 1.4398401057562558E-4 84-85 10.596501008563 5.894345432939671E-4 4.499500330488299E-6 6.749250495732449E-5 1.4623376074086972E-4 86-87 11.392975560064055 5.984335439549438E-4 4.499500330488299E-6 6.749250495732449E-5 1.4848351090611386E-4 88 12.000266370419565 6.029330442854321E-4 4.499500330488299E-6 6.749250495732449E-5 1.5298301123660218E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGGTAT 320285 0.0 44.66578 6 GGTATCA 320570 0.0 44.548485 8 TGGTATC 319890 0.0 44.534557 7 AGTGGTA 322025 0.0 44.472317 5 GTATCAA 321365 0.0 44.413185 9 AAGCAGT 325675 0.0 44.19584 1 GCAGTGG 327345 0.0 44.01033 3 CAGTGGT 328725 0.0 43.506123 4 GAGTACT 161310 0.0 42.857876 20-21 AGCAGTG 336685 0.0 42.800087 2 AGTACTT 167115 0.0 42.48772 20-21 GTACTTT 168335 0.0 41.015156 22-23 AGAGTAC 175885 0.0 40.929016 18-19 TACTTTT 179190 0.0 39.66776 22-23 ACTTTTT 194660 0.0 35.930378 24-25 CTTTTTT 259750 0.0 27.83124 24-25 ATCAACG 321875 0.0 23.32841 10-11 ACGCAGA 322240 0.0 23.326292 14-15 CAACGCA 322935 0.0 23.239838 12-13 GCAGAGT 325695 0.0 22.914684 16-17 >>END_MODULE