FastQCFastQC Report
Thu 26 May 2016
SRR1660718_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660718_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22224690
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14189226.384440007937118No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTATGGATCTCGTA362640.16316987998482768TruSeq Adapter, Index 6 (97% over 36bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT319220.14363304954984749No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC3538500.044.1705747
GGTATCA3541350.044.150278
GTGGTAT3530100.044.136946
GTATCAA3542750.044.0798349
AGTGGTA3553150.043.883735
AAGCAGT3542150.043.497091
GCAGTGG3587650.043.4834753
CAGTGGT3623750.043.0148124
GAGTACT1767450.042.87716720-21
AGTACTT1827200.042.5294420-21
AGCAGTG3670850.042.4075162
GTACTTT1841000.041.1472122-23
AGAGTAC1922000.040.9732218-19
TACTTTT1945300.039.983322-23
ACTTTTT2091150.036.42628524-25
CTTTTTT2722150.028.77311324-25
ATCAACG3542850.023.01687610-11
ACGCAGA3556050.022.93080314-15
CAACGCA3559750.022.90364812-13
GCAGAGT3592150.022.6738816-17
Produced by FastQC (version 0.11.5)