##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660718_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 22224690 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.796720179224096 34.0 31.0 34.0 30.0 34.0 2 32.23806298310573 34.0 31.0 34.0 30.0 34.0 3 31.968113885952963 34.0 31.0 34.0 30.0 34.0 4 35.80288584452696 37.0 35.0 37.0 35.0 37.0 5 35.98098380674826 37.0 35.0 37.0 35.0 37.0 6 35.73394216072305 37.0 35.0 37.0 35.0 37.0 7 35.768685682454965 37.0 35.0 37.0 35.0 37.0 8 35.98419289537897 37.0 35.0 37.0 35.0 37.0 9 37.7739563971421 39.0 38.0 39.0 35.0 39.0 10-11 37.70170823530047 39.0 37.5 39.0 35.0 39.0 12-13 37.818979949776576 39.0 38.0 39.0 35.0 39.0 14-15 39.27992568175304 41.0 39.0 41.0 36.0 41.0 16-17 39.25595907074519 41.0 39.0 41.0 36.0 41.0 18-19 39.23148592848764 41.0 39.0 41.0 36.0 41.0 20-21 39.128419564007416 40.5 39.0 41.0 36.0 41.0 22-23 38.977722883873746 40.0 38.5 41.0 35.0 41.0 24-25 39.02850791169641 40.0 39.0 41.0 35.0 41.0 26-27 39.019538585240106 40.0 39.0 41.0 35.0 41.0 28-29 38.945289338119 40.0 38.5 41.0 35.0 41.0 30-31 38.83214751251874 40.0 38.0 41.0 35.0 41.0 32-33 38.73903728240979 40.0 38.0 41.0 35.0 41.0 34-35 38.654934512022436 40.0 38.0 41.0 35.0 41.0 36-37 38.506175406721084 40.0 38.0 41.0 34.0 41.0 38-39 38.072746976448265 40.0 37.0 41.0 34.0 41.0 40-41 37.95747794457426 40.0 37.0 41.0 33.0 41.0 42-43 37.810332270101405 40.0 36.0 41.0 33.0 41.0 44-45 37.67595910224169 40.0 36.0 41.0 33.0 41.0 46-47 37.5393833389802 40.0 36.0 41.0 33.0 41.0 48-49 37.47075844477471 40.0 35.0 41.0 33.0 41.0 50-51 37.36659804928662 40.0 35.0 41.0 33.0 41.0 52-53 37.04572808439623 39.0 35.0 41.0 31.5 41.0 54-55 36.42986604087616 39.0 35.0 41.0 29.0 41.0 56-57 35.77337969618473 39.0 35.0 41.0 26.5 41.0 58-59 35.523443386611916 39.0 35.0 41.0 26.0 41.0 60-61 35.358994951110674 39.0 35.0 40.5 26.0 41.0 62-63 35.143674827410415 38.0 35.0 40.0 26.0 41.0 64-65 34.95127774560635 37.5 35.0 40.0 26.0 41.0 66-67 34.752863527005324 37.0 34.5 40.0 26.0 41.0 68-69 34.50633995344817 36.5 34.0 39.5 26.0 41.0 70-71 34.21944875271601 36.0 34.0 39.0 26.0 41.0 72-73 33.86029746646635 35.5 34.0 39.0 25.0 40.5 74-75 33.46195211271788 35.0 34.0 37.5 24.5 40.0 76-77 31.799542198338873 34.5 31.0 36.0 21.0 39.0 78-79 32.68144394814956 35.0 33.0 37.0 23.5 39.0 80-81 32.60236777205891 35.0 33.0 36.0 23.5 39.0 82-83 32.35198967904614 35.0 33.0 36.0 22.5 37.0 84-85 32.124209449040684 35.0 33.0 35.5 21.0 37.0 86-87 31.547391414683396 35.0 33.0 35.0 15.5 36.5 88-89 31.309247620551737 35.0 32.5 35.0 15.5 36.0 90-91 31.53244558191813 35.0 33.0 35.0 20.0 36.0 92-93 31.48421730516826 35.0 33.0 35.0 19.0 36.0 94-95 31.404354143972313 35.0 33.0 35.0 19.0 35.5 96-97 31.31335658675104 35.0 33.0 35.0 19.0 35.0 98-99 31.223829893690308 35.0 33.0 35.0 19.0 35.0 100 31.14933981081401 35.0 33.0 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9794591077142947 1101 2 0.7769500158960625 1101 3 1.136851549436507 1101 4 0.660353030107494 1101 5 0.4220470212516787 1101 6 0.68298791725082 1101 7 0.5475179862456159 1101 8 0.3809055271176689 1101 9 0.4280834357615788 1101 10-11 0.4071737291069297 1101 12-13 0.37076418579858483 1101 14-15 0.4623214504865345 1101 16-17 0.4704612431661772 1101 18-19 0.4745059926800863 1101 20-21 0.5021302170188875 1101 22-23 0.47225604936813426 1101 24-25 0.4743635182439334 1101 26-27 0.44430025554745356 1101 28-29 0.4570075926590178 1101 30-31 0.4601386079695047 1101 32-33 0.4459878795987251 1101 34-35 0.45763756481841256 1101 36-37 0.4642945284786606 1101 38-39 0.4775945238951351 1101 40-41 0.46710217282621613 1101 42-43 0.4376733300355298 1101 44-45 0.44757062593558317 1101 46-47 0.467822149054534 1101 48-49 0.3987806103452911 1101 50-51 0.4192626209774275 1101 52-53 0.4181293408462494 1101 54-55 0.39606388098819423 1101 56-57 0.4402639880961985 1101 58-59 0.41654933245653325 1101 60-61 0.46871660091233736 1101 62-63 0.4633249910397339 1101 64-65 0.3875512496221205 1101 66-67 0.3793061164213256 1101 68-69 0.39628558133644276 1101 70-71 0.38202099620471586 1101 72-73 0.3925370947955926 1101 74-75 0.3824195894918958 1101 76-77 0.3117226788003542 1101 78-79 0.39569806527696727 1101 80-81 0.4288635952219977 1101 82-83 0.4071467647006486 1101 84-85 0.36769172625866986 1101 86-87 0.7485181956794698 1101 88-89 0.8268513604791536 1101 90-91 0.4738063302219757 1101 92-93 0.3616093131874223 1101 94-95 0.3590524985658341 1101 96-97 0.36456376419852177 1101 98-99 0.34995195681379343 1101 100 0.3631037822917236 1102 1 0.883179794656197 1102 2 0.6890211801624417 1102 3 1.0526279207571037 1102 4 0.6150036419635612 1102 5 0.36210758281953304 1102 6 0.6262112843927738 1102 7 0.4920328575557775 1102 8 0.32679950087749177 1102 9 0.38691960154800853 1102 10-11 0.3431881325742765 1102 12-13 0.31965843562571195 1102 14-15 0.4036948336672097 1102 16-17 0.4085883090140072 1102 18-19 0.3856116082654779 1102 20-21 0.4191586825950466 1102 22-23 0.42952494638049643 1102 24-25 0.3960780145347229 1102 26-27 0.3926350825378151 1102 28-29 0.39471470135502784 1102 30-31 0.4000698561253344 1102 32-33 0.3907268460340063 1102 34-35 0.40016271870450737 1102 36-37 0.40667774846667726 1102 38-39 0.35886697532070144 1102 40-41 0.38782754434351574 1102 42-43 0.37600219559260495 1102 44-45 0.34878959257950015 1102 46-47 0.39842973371516877 1102 48-49 0.4016757428613289 1102 50-51 0.40310324119002416 1102 52-53 0.3540615481590166 1102 54-55 0.451684807970814 1102 56-57 0.48713207244240664 1102 58-59 0.4669727963338133 1102 60-61 0.4004848744447358 1102 62-63 0.38258824897676647 1102 64-65 0.42015515766972555 1102 66-67 0.38761861586248614 1102 68-69 0.3761199656318297 1102 70-71 0.34297876582300546 1102 72-73 0.3265462846516627 1102 74-75 0.33733870102457786 1102 76-77 0.2368965388025579 1102 78-79 0.36511349321110487 1102 80-81 0.3469530781006114 1102 82-83 0.39765561198736066 1102 84-85 0.40522004551169744 1102 86-87 0.7877893877574884 1102 88-89 0.8333179974092815 1102 90-91 0.4507847939912182 1102 92-93 0.37909271468690164 1102 94-95 0.3130059799199323 1102 96-97 0.3080214369510905 1102 98-99 0.32853991675832717 1102 100 0.37271556310098575 1103 1 0.8926016857717691 1103 2 0.7028576116133891 1103 3 1.03833704750798 1103 4 0.6177340833777336 1103 5 0.39219791146629035 1103 6 0.6403196011368877 1103 7 0.5220739513976156 1103 8 0.34494060951792704 1103 9 0.36081688054633787 1103 10-11 0.3368748031421802 1103 12-13 0.30679496016574603 1103 14-15 0.3974661326481552 1103 16-17 0.3836993245099123 1103 18-19 0.37202846638398057 1103 20-21 0.4299498016499612 1103 22-23 0.4146203817574019 1103 24-25 0.3736041347575778 1103 26-27 0.3947048288267325 1103 28-29 0.4106718575873529 1103 30-31 0.4190899674059594 1103 32-33 0.3896317885932561 1103 34-35 0.366781358512128 1103 36-37 0.3395978718946324 1103 38-39 0.35469843572422377 1103 40-41 0.3596862035002104 1103 42-43 0.35985327705144954 1103 44-45 0.3805643233974578 1103 46-47 0.35664149267612544 1103 48-49 0.33852692863817424 1103 50-51 0.30061276367350587 1103 52-53 0.29603191898698356 1103 54-55 0.3564787530558746 1103 56-57 0.39858304835291136 1103 58-59 0.3991404590179144 1103 60-61 0.3818481036657104 1103 62-63 0.40332509104555214 1103 64-65 0.36981810394976833 1103 66-67 0.3513456910282926 1103 68-69 0.35714098804876215 1103 70-71 0.3431342572647722 1103 72-73 0.3215491468985263 1103 74-75 0.3367249663291716 1103 76-77 0.28987936220223176 1103 78-79 0.38908309950248565 1103 80-81 0.38494690341058657 1103 82-83 0.39928017643370595 1103 84-85 0.39030042669175913 1103 86-87 0.755472954951447 1103 88-89 0.8061635295662626 1103 90-91 0.4680458711075204 1103 92-93 0.41480679307720436 1103 94-95 0.4248228356297652 1103 96-97 0.3936558277374189 1103 98-99 0.3472049872613532 1103 100 0.3689168802110778 1104 1 0.8415223750688021 1104 2 0.6568759157766806 1104 3 1.0054647061091941 1104 4 0.577893962230263 1104 5 0.35161119161831067 1104 6 0.5806437583529984 1104 7 0.4735821970705274 1104 8 0.30584372780820956 1104 9 0.3279697234077048 1104 10-11 0.2725716269623035 1104 12-13 0.2433808654588745 1104 14-15 0.31439011220520996 1104 16-17 0.32644359314817706 1104 18-19 0.29418538089644386 1104 20-21 0.33990447498483434 1104 22-23 0.3349880829830596 1104 24-25 0.33621242860783696 1104 26-27 0.30818530624148366 1104 28-29 0.30211043286541184 1104 30-31 0.3287442624082004 1104 32-33 0.3181524001105913 1104 34-35 0.34074997958764897 1104 36-37 0.29683056422754106 1104 38-39 0.32613644962760446 1104 40-41 0.2987914741773565 1104 42-43 0.3263792447243574 1104 44-45 0.33918475117027214 1104 46-47 0.31895912606101007 1104 48-49 0.3149718675238944 1104 50-51 0.3176803175081844 1104 52-53 0.31701010138201013 1104 54-55 0.2982707243076135 1104 56-57 0.31294743050443685 1104 58-59 0.3554491192504017 1104 60-61 0.3485723546307753 1104 62-63 0.3040579116634916 1104 64-65 0.2867621880307496 1104 66-67 0.29311518380901447 1104 68-69 0.28489309163134635 1104 70-71 0.2623692411253984 1104 72-73 0.24334729929584853 1104 74-75 0.2879639527973268 1104 76-77 0.19406795035576252 1104 78-79 0.2756559690182243 1104 80-81 0.29914316160846255 1104 82-83 0.3376597289341703 1104 84-85 0.31133911002167736 1104 86-87 0.6766947436279729 1104 88-89 0.7502041009447993 1104 90-91 0.4258314455061267 1104 92-93 0.356567507358978 1104 94-95 0.34620633605017304 1104 96-97 0.3846931717527582 1104 98-99 0.3290203892176642 1104 100 0.35737732763874064 1105 1 0.8140255112693389 1105 2 0.6349811918755393 1105 3 0.9874154571416369 1105 4 0.571290997317206 1105 5 0.3249458057432122 1105 6 0.5651691383280095 1105 7 0.44230085500885963 1105 8 0.2715972424422475 1105 9 0.28212290741674906 1105 10-11 0.23118593000485532 1105 12-13 0.23052899959893125 1105 14-15 0.27174906563092804 1105 16-17 0.2886937568680352 1105 18-19 0.24418400322864642 1105 20-21 0.31189953169879914 1105 22-23 0.28622813972310013 1105 24-25 0.300455190067062 1105 26-27 0.29480808750129484 1105 28-29 0.3139440450665987 1105 30-31 0.32529995201090145 1105 32-33 0.3206028860900645 1105 34-35 0.29682574820159857 1105 36-37 0.2719100156283716 1105 38-39 0.2711884110147693 1105 40-41 0.26495025912252146 1105 42-43 0.31115745348986223 1105 44-45 0.30498341260837236 1105 46-47 0.2829875440992282 1105 48-49 0.31926729621006444 1105 50-51 0.2917953990667925 1105 52-53 0.31349283048190557 1105 54-55 0.291501166206686 1105 56-57 0.2793581843919668 1105 58-59 0.27450448464258415 1105 60-61 0.2145531973480601 1105 62-63 0.22589596775092247 1105 64-65 0.2564564320525591 1105 66-67 0.23513841373377176 1105 68-69 0.23897971384081984 1105 70-71 0.2814686847214958 1105 72-73 0.2328464015475049 1105 74-75 0.259161187476046 1105 76-77 0.14683044226291386 1105 78-79 0.23941960398838802 1105 80-81 0.23237041022264293 1105 82-83 0.2596407897089392 1105 84-85 0.2713191409085667 1105 86-87 0.6218469848039838 1105 88-89 0.7155821605018104 1105 90-91 0.3462169219220854 1105 92-93 0.2531111815393601 1105 94-95 0.2620024948525064 1105 96-97 0.23817169359661605 1105 98-99 0.28395552389845236 1105 100 0.3265027080761591 1106 1 0.7458078462215703 1106 2 0.5827828399793518 1106 3 0.9189090670749707 1106 4 0.511688235148199 1106 5 0.2602767212520476 1106 6 0.502860654058864 1106 7 0.37969918392983715 1106 8 0.23510275708630957 1106 9 0.23724706724051003 1106 10-11 0.18747230371128865 1106 12-13 0.1690671574833118 1106 14-15 0.22641649959457055 1106 16-17 0.22610860134179944 1106 18-19 0.1768120251938754 1106 20-21 0.2390854377228493 1106 22-23 0.2402559903609074 1106 24-25 0.21201849357764502 1106 26-27 0.18441875939571872 1106 28-29 0.2148720430744362 1106 30-31 0.24986477255721695 1106 32-33 0.2280555350931337 1106 34-35 0.2266144054736614 1106 36-37 0.211754742085013 1106 38-39 0.23834623671150013 1106 40-41 0.207346701043285 1106 42-43 0.2141819212902547 1106 44-45 0.2600483917013179 1106 46-47 0.24513215328740046 1106 48-49 0.25801476839433946 1106 50-51 0.2465215244611798 1106 52-53 0.24344201727369352 1106 54-55 0.2532069827171597 1106 56-57 0.21959074929243627 1106 58-59 0.20209504516945032 1106 60-61 0.21161524651223118 1106 62-63 0.21345084856194063 1106 64-65 0.18644093223307578 1106 66-67 0.1917885038378202 1106 68-69 0.1890452326831138 1106 70-71 0.1640369959925465 1106 72-73 0.12234837286373335 1106 74-75 0.155312096471782 1106 76-77 0.08046769252561248 1106 78-79 0.15506315837521356 1106 80-81 0.1921188656185251 1106 82-83 0.17757324175045142 1106 84-85 0.19190193812468692 1106 86-87 0.5756955304285931 1106 88-89 0.6631544173634509 1106 90-91 0.32492113989149374 1106 92-93 0.26301643347531467 1106 94-95 0.24179684766666298 1106 96-97 0.24008511489534712 1106 98-99 0.2512663809824467 1106 100 0.2512281050613865 1107 1 0.7086542133488045 1107 2 0.5262219691929104 1107 3 0.8865246857001736 1107 4 0.4868380492482487 1107 5 0.2608279217386098 1107 6 0.5158455832828039 1107 7 0.3803749229988398 1107 8 0.18779594102164765 1107 9 0.1697111687324906 1107 10-11 0.14641470443409332 1107 12-13 0.12363121084162998 1107 14-15 0.14039191717376553 1107 16-17 0.15790272165686048 1107 18-19 0.13974143940718164 1107 20-21 0.18017586707169642 1107 22-23 0.1765952005007918 1107 24-25 0.16479670728926266 1107 26-27 0.14435360216437942 1107 28-29 0.16042455088618368 1107 30-31 0.13182387570819998 1107 32-33 0.10019959461924088 1107 34-35 0.13007115345871512 1107 36-37 0.130647129463064 1107 38-39 0.1078107960168424 1107 40-41 0.1389449174861923 1107 42-43 0.15101468575050347 1107 44-45 0.13850244004402867 1107 46-47 0.19218156278688525 1107 48-49 0.17906431536454903 1107 50-51 0.13164220734684307 1107 52-53 0.14232617519499513 1107 54-55 0.13965838170663147 1107 56-57 0.15381060964787707 1107 58-59 0.12131707922370794 1107 60-61 0.08667501747719797 1107 62-63 0.10944526860134118 1107 64-65 0.08246030243766 1107 66-67 0.058275908271333776 1107 68-69 0.06962187645417828 1107 70-71 0.07352929148429865 1107 72-73 0.03628034672694014 1107 74-75 0.04443276281506314 1107 76-77 -0.07050812633873349 1107 78-79 0.07626385631662913 1107 80-81 0.1240693736445948 1107 82-83 0.14599330724529835 1107 84-85 0.0701775348922169 1107 86-87 0.45014961317575697 1107 88-89 0.5580508788325957 1107 90-91 0.20906375970082536 1107 92-93 0.10594272636474855 1107 94-95 0.1161147594184051 1107 96-97 0.10629483447648269 1107 98-99 0.1286122263301337 1107 100 0.1349970219144474 1108 1 0.6729643282766169 1108 2 0.5068807711959025 1108 3 0.8564823219925728 1108 4 0.4792496326734579 1108 5 0.24731395170037018 1108 6 0.48505046118262385 1108 7 0.32862133188797316 1108 8 0.15427544557762474 1108 9 0.12987416356349257 1108 10-11 0.08550964805964156 1108 12-13 0.11350237455398826 1108 14-15 0.12558553208626932 1108 16-17 0.10673045432834982 1108 18-19 0.10220297693917502 1108 20-21 0.16616466619295522 1108 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-0.35273142705403515 2316 88-89 -0.2148185309336661 2316 90-91 -0.5672950783932151 2316 92-93 -0.7429077232196164 2316 94-95 -0.795156200566101 2316 96-97 -0.8310003327375703 2316 98-99 -0.9203636648283577 2316 100 -0.8661727583512437 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 31.0 7 558.0 8 830.0 9 371.0 10 224.0 11 364.0 12 526.0 13 965.0 14 2579.0 15 6763.0 16 15105.0 17 24531.0 18 32069.0 19 41662.0 20 58394.0 21 83009.0 22 124252.0 23 211239.0 24 347000.0 25 428000.0 26 423471.0 27 353041.0 28 306844.0 29 313596.0 30 357925.0 31 422360.0 32 510160.0 33 647537.0 34 885824.0 35 1367200.0 36 2511841.0 37 4995455.0 38 6121677.0 39 1626307.0 40 2980.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 14.847159917367767 33.10994331721182 28.70884543356322 23.33405133185719 2 21.169964865093007 34.840005773924574 25.99398165555048 17.996047705431934 3 26.641780621480994 25.310247001191495 26.26304054444097 21.78493183288655 4 19.23842204894371 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20.25386566006453 24-25 20.303980702134833 29.953295744863656 25.35134604800812 24.39137750499339 26-27 20.4832154041896 25.99874556181015 33.00198343780723 20.516055596193024 28-29 20.60875806995742 26.233483662438623 32.90110800173731 20.256650265866647 30-31 20.520185079664703 26.107141175406294 33.04686366480028 20.32581008012873 32-33 20.46855944228071 26.30410847856664 32.77079846190935 20.4565336172433 34-35 20.500025187459837 26.44462794004534 32.64940736573121 20.405939506763616 36-37 20.474213673104813 26.221058159249782 32.69135234405374 20.613375823591664 38-39 20.430854488209135 26.331104408425226 32.80180281827031 20.43623828509533 40-41 20.598121969586472 26.246911442061688 32.85373855366473 20.301228034687107 42-43 20.66374777405489 26.3447975422119 32.74506613385132 20.246388549881893 44-45 20.44521691967673 26.443325638932784 32.70779145472863 20.40366598666185 46-47 20.41952885875366 26.477130529819913 32.60616455894783 20.497176052478594 48-49 20.767488921577165 26.459738177258817 32.465322336931216 20.307450564232802 50-51 20.696739235391167 26.628395862358772 32.37830569892955 20.29655920332051 52-53 20.81904308907707 26.60758088084883 32.09020708645473 20.48316894361937 54-55 21.157405512299462 26.876984817188603 31.351332302226247 20.614277368285684 56-57 21.502967633250964 27.565108689681733 30.061260857598903 20.870662819468397 58-59 21.548598120157582 28.017780940562087 29.395558104265252 21.038062835015083 60-61 21.673544904334037 28.428985643698024 28.763239708336048 21.134229743631888 62-63 21.88109194251371 28.610279938526478 28.38401373478632 21.124614384173494 64-65 21.670491409293515 28.67283756147792 28.520099138769073 21.13657189045949 66-67 21.669866300816736 28.7541490772301 28.429203427804282 21.146781194148875 68-69 21.568800637615563 28.622029016476912 28.703225176651493 21.10594516925603 70-71 21.371247685258012 28.619597541966012 28.770853831990294 21.238300940785678 72-73 21.41126522265572 28.388234881870826 28.873213745543385 21.327286149930067 74-75 21.60555924111378 28.4309145073969 28.753362968732226 21.21016328275709 76-77 21.545155485417908 28.578025036097763 28.581536119846433 21.295283358637896 78-79 21.589953197984666 28.721441288343513 28.22594203961284 21.46266347405898 80-81 21.741968700565874 28.974667625083605 27.762541625215313 21.52082204913521 82-83 21.771726782443814 29.017297926870782 27.443992876695372 21.766982413990036 84-85 21.668523502591242 29.104604457936702 27.269917772553132 21.956954266918927 86-87 21.956934743914452 29.054516666895886 27.02633203789184 21.962216551297818 88-89 21.727725602492495 29.149268622161294 26.936231734739845 22.186774040606362 90-91 21.63919649349651 29.14475417046315 26.760527405000538 22.455521931039797 92-93 21.60358107727807 29.084446848160805 26.715810773680875 22.59616130088025 94-95 21.77789416758841 29.017937791861787 26.65544244183402 22.548725598715784 96-97 21.805402873582384 28.94331681464796 26.60540002887023 22.645880282899423 98-99 21.80834308722824 28.893100237185383 26.60825176133807 22.690304914248312 100 21.745651844262387 28.94746125660835 26.6076848278354 22.69920207129387 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 19235.0 1 14727.5 2 8470.5 3 6219.0 4 5654.0 5 5615.5 6 5667.0 7 5720.0 8 5721.5 9 5921.5 10 6513.0 11 11054.5 12 102406.0 13 124058.5 14 56428.0 15 58003.0 16 65846.5 17 73346.5 18 80053.5 19 87514.5 20 96047.5 21 108493.5 22 126763.5 23 147859.5 24 170597.5 25 196846.0 26 223583.0 27 253833.0 28 291973.5 29 329389.0 30 360195.5 31 392016.0 32 426915.5 33 452788.5 34 475607.0 35 507258.5 36 538966.5 37 562777.5 38 588647.0 39 610804.5 40 625932.0 41 653079.0 42 690171.5 43 700599.0 44 694557.5 45 705727.0 46 721802.5 47 797453.0 48 896119.5 49 914848.5 50 844115.0 51 748165.0 52 695802.5 53 662312.0 54 625014.0 55 584400.0 56 540030.5 57 489888.0 58 433515.5 59 369450.5 60 302832.5 61 244287.5 62 191272.0 63 140881.0 64 100629.5 65 71259.0 66 49721.5 67 35630.5 68 25263.0 69 17599.5 70 12796.0 71 9649.5 72 7636.5 73 5970.5 74 4867.5 75 4453.0 76 3301.5 77 1587.5 78 700.5 79 431.0 80 285.0 81 208.5 82 145.0 83 107.0 84 81.0 85 55.0 86 41.0 87 29.0 88 14.5 89 13.0 90 11.0 91 7.0 92 6.0 93 5.0 94 4.5 95 1.0 96 1.0 97 1.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2546042262006804 2 0.002870681210851535 3 1.165217602585233 4 4.3645153205736504E-4 5 2.6997001982929797E-5 6 0.004521997832140741 7 0.00378407977794066 8 1.709810125585554E-4 9 0.0 10-11 3.5996002643906394E-5 12-13 2.2497501652441496E-6 14-15 0.0 16-17 2.0247751487197348E-5 18-19 0.0 20-21 1.4623376074086972E-4 22-23 9.853905723769376E-4 24-25 2.497222683421006E-4 26-27 0.0017930508816995875 28-29 0.003025913972253381 30-31 0.0028729309610167793 32-33 0.007570409306046563 34-35 0.005982085689384194 36-37 0.002769442453415548 38-39 0.0026884514474667586 40-41 0.004177786056858386 42-43 0.009480447196338846 44-45 0.0169586167456104 46-47 0.01650416721223108 48-49 0.021125154051642565 50-51 0.023917094006710556 52-53 0.038832937602279266 54-55 0.02779116379126098 56-57 0.021194896306765135 58-59 0.02325341770796353 60-61 0.023462644473331237 62-63 0.016828131236026236 64-65 0.023982336761502635 66-67 0.025842880148159546 68-69 0.025462672370233287 70-71 0.030466116737736276 72-73 0.03530082984284595 74-75 0.033030831926114605 76-77 0.04196683958246437 78-79 0.04328969267962793 80-81 0.039996058437710494 82-83 0.039944314183909875 84-85 0.04265526313302907 86-87 0.5422527828284669 88-89 0.03488687581244103 90-91 0.03047961523872774 92-93 0.03258313164323102 94-95 0.03671367294661928 96-97 0.03613548715415153 98-99 0.024121821271747772 100 0.00404955029743947 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.222469E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.674072690464435 #Duplication Level Percentage of deduplicated Percentage of total 1 72.066224709271 30.032930870615036 2 14.6511216187304 12.211438146716116 3 5.023008941302505 6.279877192340802 4 2.3662313647993027 3.944419915964121 5 1.3737494672514514 2.862486757836189 6 0.8669985315987536 2.1678815897023433 7 0.6153582846942101 1.7951140120918214 8 0.45501133750625045 1.5169740443376742 9 0.36107903784058365 1.35428706629743 >10 1.9344763826784743 14.796061829890498 >50 0.16142187166539745 4.757325408764205 >100 0.12306532753271227 8.27536656123697 >500 9.349566470999617E-4 0.2441563461864873 >1k 7.997714310616256E-4 0.6662096106256693 >5k 1.9439889404556775E-4 0.6424315142145989 >10k+ 3.239981567426111E-4 8.45303913318009 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1418922 6.384440007937118 No Hit AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTATGGATCTCGTA 36264 0.16316987998482768 TruSeq Adapter, Index 6 (97% over 36bp) AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 31922 0.14363304954984749 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.18929397890364275 0.0 0.0 4.499500330488299E-6 4.499500330488299E-6 2 0.19652467593473744 4.499500330488299E-6 0.0 4.499500330488299E-6 4.499500330488299E-6 3 0.20476776054019202 4.499500330488299E-6 0.0 4.499500330488299E-6 4.499500330488299E-6 4 0.2057756486142214 4.499500330488299E-6 0.0 1.3498500991464899E-5 4.499500330488299E-6 5 0.20586113912050066 2.2497501652441495E-5 0.0 1.3498500991464899E-5 4.499500330488299E-6 6 0.20596012812777142 3.149650231341809E-5 0.0 1.3498500991464899E-5 8.999000660976599E-6 7 0.20610411213834703 4.499500330488299E-5 0.0 1.3498500991464899E-5 8.999000660976599E-6 8 0.2074629612381545 4.949450363537129E-5 0.0 1.3498500991464899E-5 8.999000660976599E-6 9 0.20866432782639488 4.949450363537129E-5 0.0 1.7998001321953197E-5 8.999000660976599E-6 10-11 0.2089455465970504 6.074325446159204E-5 0.0 1.7998001321953197E-5 8.999000660976599E-6 12-13 0.20908503110729554 6.524275479208034E-5 0.0 2.0247751487197345E-5 8.999000660976599E-6 14-15 0.2091547733624181 7.649150561830109E-5 0.0 2.2497501652441495E-5 8.999000660976599E-6 16-17 0.20923576436836688 8.099100594878939E-5 0.0 2.2497501652441495E-5 8.999000660976599E-6 18-19 0.20934600212646387 9.448950694025428E-5 0.0 2.2497501652441495E-5 1.1248750826220748E-5 20-21 0.2094449911337346 1.2148650892318408E-4 0.0 2.2497501652441495E-5 1.3498500991464899E-5 22-23 0.20963172039744987 1.3723476007989314E-4 0.0 2.2497501652441495E-5 1.3498500991464899E-5 24-25 0.2100321759268633 1.4398401057562558E-4 0.0 2.4747251817685646E-5 2.0247751487197345E-5 26-27 0.21116829976031162 1.5298301123660218E-4 0.0 2.6997001982929797E-5 2.4747251817685646E-5 28-29 0.21437644349594978 1.6873126239331122E-4 0.0 2.6997001982929797E-5 2.6997001982929797E-5 30-31 0.2218276160432384 1.9347851421099688E-4 4.499500330488299E-6 2.6997001982929797E-5 2.6997001982929797E-5 32-33 0.2369369381530181 2.0922676536770592E-4 8.999000660976599E-6 2.6997001982929797E-5 2.9246752148173945E-5 34-35 0.2644581319244498 2.227252663591708E-4 1.3498500991464899E-5 2.6997001982929797E-5 3.149650231341809E-5 36-37 0.30858473166554856 2.2947451685490327E-4 1.3498500991464899E-5 2.6997001982929797E-5 3.374625247866224E-5 38-39 0.37400071722035266 2.4297301784636816E-4 1.3498500991464899E-5 2.6997001982929797E-5 3.8245752809150545E-5 40-41 0.46249239022006605 2.519720185073448E-4 1.574825115670905E-5 3.5996002643906394E-5 4.0495502974394696E-5 42-43 0.5753578565100346 2.8346852082076286E-4 1.7998001321953197E-5 3.5996002643906394E-5 4.0495502974394696E-5 44-45 0.7163024546124153 2.9696702181222773E-4 1.7998001321953197E-5 3.5996002643906394E-5 4.0495502974394696E-5 46-47 0.8885140805113592 3.0371627230796024E-4 1.7998001321953197E-5 3.5996002643906394E-5 4.0495502974394696E-5 48-49 1.0953403624527496 3.082157726384485E-4 1.7998001321953197E-5 3.5996002643906394E-5 4.274525313963885E-5 50-51 1.3343110747551483 3.3296302445613414E-4 1.7998001321953197E-5 3.5996002643906394E-5 4.499500330488299E-5 52-53 1.599189909960499 3.5546052610857564E-4 1.7998001321953197E-5 4.0495502974394696E-5 4.724475347012714E-5 54-55 1.8955809957304242 3.5546052610857564E-4 1.7998001321953197E-5 4.0495502974394696E-5 5.3994003965859594E-5 56-57 2.2351987811753506 3.847072782567496E-4 1.7998001321953197E-5 4.0495502974394696E-5 6.074325446159204E-5 58-59 2.6178745350328847 4.1620378057016766E-4 1.7998001321953197E-5 4.0495502974394696E-5 6.749250495732449E-5 60-61 3.042139170445122 4.387012822226092E-4 1.7998001321953197E-5 4.499500330488299E-5 8.099100594878939E-5 62-63 3.4976055908991306 4.477002828835858E-4 1.7998001321953197E-5 4.499500330488299E-5 8.099100594878939E-5 64-65 3.9866562818199043 4.5444953337931825E-4 1.7998001321953197E-5 4.724475347012714E-5 8.54905062792777E-5 66-67 4.510483610795021 4.634485340402948E-4 1.7998001321953197E-5 4.949450363537129E-5 9.448950694025428E-5 68-69 5.071355325991048 4.679480343707831E-4 2.2497501652441495E-5 4.949450363537129E-5 9.898900727074259E-5 70-71 5.669570644179964 4.701977845360273E-4 2.2497501652441495E-5 4.949450363537129E-5 1.0123875743598673E-4 72-73 6.298569293879915 4.7919678519700386E-4 2.2497501652441495E-5 5.3994003965859594E-5 1.0348850760123089E-4 74-75 6.9536807937478535 4.949450363537129E-4 2.2497501652441495E-5 5.3994003965859594E-5 1.0348850760123089E-4 76-77 7.627892672518717 5.039440370146895E-4 2.2497501652441495E-5 5.849350429634789E-5 1.0348850760123089E-4 78-79 8.324426572429132 5.129430376756662E-4 2.2497501652441495E-5 6.299300462683618E-5 1.1698700859269578E-4 80-81 9.041424199842607 5.129430376756662E-4 2.2497501652441495E-5 6.299300462683618E-5 1.1698700859269578E-4 82-83 9.78441319091515 5.331907891628635E-4 2.2497501652441495E-5 6.299300462683618E-5 1.2148650892318408E-4 84-85 10.546858021416721 5.624375413110374E-4 2.2497501652441495E-5 6.299300462683618E-5 1.3048550958416068E-4 86-87 11.332243554353289 5.71436541972014E-4 2.2497501652441495E-5 6.299300462683618E-5 1.3048550958416068E-4 88 11.939262144938805 5.71436541972014E-4 2.2497501652441495E-5 6.299300462683618E-5 1.3498500991464897E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGTATC 353850 0.0 44.170574 7 GGTATCA 354135 0.0 44.15027 8 GTGGTAT 353010 0.0 44.13694 6 GTATCAA 354275 0.0 44.079834 9 AGTGGTA 355315 0.0 43.88373 5 AAGCAGT 354215 0.0 43.49709 1 GCAGTGG 358765 0.0 43.483475 3 CAGTGGT 362375 0.0 43.014812 4 GAGTACT 176745 0.0 42.877167 20-21 AGTACTT 182720 0.0 42.52944 20-21 AGCAGTG 367085 0.0 42.407516 2 GTACTTT 184100 0.0 41.14721 22-23 AGAGTAC 192200 0.0 40.97322 18-19 TACTTTT 194530 0.0 39.9833 22-23 ACTTTTT 209115 0.0 36.426285 24-25 CTTTTTT 272215 0.0 28.773113 24-25 ATCAACG 354285 0.0 23.016876 10-11 ACGCAGA 355605 0.0 22.930803 14-15 CAACGCA 355975 0.0 22.903648 12-13 GCAGAGT 359215 0.0 22.67388 16-17 >>END_MODULE