FastQCFastQC Report
Thu 26 May 2016
SRR1660717_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660717_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35272682
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21516926.100165561552705No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT491350.13930043652478707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2668200.087.083048
GTGGTAT2693250.086.272496
TGGTATC2704850.085.661097
AGTGGTA2716100.085.560765
GTATCAA2720800.085.371069
GCAGTGG2797650.083.63363
AAGCAGT2817500.083.00451
CAGTGGT2867800.081.0018844
AGCAGTG2935700.079.722812
CAACGCA2637900.045.4511412-13
ATCAACG2651500.045.27346410-11
ACGCAGA2659500.045.0855314-15
TCAACGC2632250.044.35038412-13
AGAGTAC2663850.044.33369418-19
AACGCAG2650700.044.1151714-15
CGCAGAG2666700.043.76857816-17
GAGTACT2629500.043.46707520-21
TATCAAC2701800.042.9449110-11
AGTACTT2772250.042.30895220-21
CAGAGTA2789300.041.4017718-19
Produced by FastQC (version 0.11.5)