##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660717_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 35272682 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.89865071785582 34.0 31.0 34.0 30.0 34.0 2 32.02970888349233 34.0 31.0 34.0 30.0 34.0 3 32.092586665227216 34.0 31.0 34.0 30.0 34.0 4 35.496955377535514 37.0 35.0 37.0 33.0 37.0 5 35.459681007528715 37.0 35.0 37.0 33.0 37.0 6 35.54265675062645 37.0 35.0 37.0 33.0 37.0 7 35.48983349210587 37.0 35.0 37.0 33.0 37.0 8 35.50478880511553 37.0 35.0 37.0 33.0 37.0 9 37.18973697548715 39.0 37.0 39.0 34.0 39.0 10-11 37.117625064632165 39.0 37.0 39.0 33.5 39.0 12-13 37.15517449169303 39.0 37.0 39.0 33.5 39.0 14-15 38.54442257892383 40.0 38.0 41.0 34.0 41.0 16-17 38.52283780121965 40.0 38.0 41.0 34.0 41.0 18-19 38.49075531029934 40.0 38.0 41.0 34.0 41.0 20-21 38.371300515225926 40.0 38.0 41.0 33.5 41.0 22-23 38.1898946896071 40.0 38.0 41.0 33.5 41.0 24-25 38.18688349244324 40.0 38.0 41.0 33.0 41.0 26-27 38.1874228191664 40.0 38.0 41.0 33.0 41.0 28-29 38.11722737726606 40.0 38.0 41.0 33.0 41.0 30-31 38.037066163553995 40.0 38.0 41.0 33.0 41.0 32-33 37.92119368751149 40.0 38.0 41.0 32.5 41.0 34-35 37.776121121155455 40.0 38.0 41.0 32.0 41.0 36-37 37.59310254604399 40.0 37.5 41.0 31.5 41.0 38-39 37.15326287068275 40.0 35.0 41.0 31.0 41.0 40-41 37.100685680776984 40.0 35.0 41.0 31.0 41.0 42-43 37.11650852067331 40.0 35.0 41.0 31.0 41.0 44-45 37.1248149630357 40.0 35.0 41.0 31.0 41.0 46-47 37.21924461258716 40.0 35.0 41.0 31.0 41.0 48-49 37.123685406173536 40.0 35.0 41.0 31.0 41.0 50-51 35.77915009411532 38.5 34.0 40.0 29.0 40.5 52-53 35.94294398991265 38.5 35.0 39.5 29.0 40.5 54-55 36.29520837116951 39.5 35.0 41.0 27.5 41.0 56-57 35.890349321891655 40.0 35.0 41.0 25.5 41.0 58-59 35.70904423995884 39.0 35.0 41.0 25.0 41.0 60-61 35.513948669965046 39.0 35.0 41.0 25.0 41.0 62-63 35.31715580913297 39.0 35.0 41.0 25.0 41.0 64-65 35.09631772542842 38.5 35.0 41.0 25.0 41.0 66-67 34.865271912127355 37.5 34.0 40.0 24.5 41.0 68-69 34.62931236700402 37.0 34.0 40.0 25.0 41.0 70-71 34.36279822725133 36.5 34.0 39.5 24.5 41.0 72-73 34.03817973070491 36.0 34.0 39.0 24.0 41.0 74-75 33.667499142821065 35.5 34.0 39.0 24.0 41.0 76-77 33.27418575655801 35.0 33.0 37.5 23.0 40.0 78-79 32.896763620072896 35.0 33.0 37.0 22.0 39.0 80-81 32.56471457146355 35.0 33.0 37.0 20.5 39.0 82-83 32.238130928064955 35.0 33.0 36.0 20.0 38.5 84-85 31.94340513998907 35.0 33.0 36.0 20.0 37.0 86-87 31.72438813980746 35.0 32.0 35.5 20.0 37.0 88-89 31.51751850057787 35.0 32.0 35.0 19.0 36.5 90-91 31.34565659055923 35.0 32.0 35.0 19.0 36.0 92-93 31.192776891760033 35.0 32.0 35.0 18.5 36.0 94-95 31.014979836237007 35.0 32.0 35.0 18.0 36.0 96-97 30.888070263554102 35.0 31.5 35.0 18.0 35.5 98-99 30.798947114370264 35.0 31.0 35.0 18.0 35.0 100 30.731990354461846 35.0 31.0 35.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5177576233310326 1101 2 0.5365613855879303 1101 3 0.547811836611416 1101 4 0.48274059665005353 1101 5 0.4840785499310485 1101 6 0.46299021973733545 1101 7 0.4885754065463459 1101 8 0.5060072672622979 1101 9 0.561275210203398 1101 10-11 0.5983616855129981 1101 12-13 0.5914049540314963 1101 14-15 0.6944421776064971 1101 16-17 0.6616467182254624 1101 18-19 0.6514899214854779 1101 20-21 0.6866218136770499 1101 22-23 0.7522243062119074 1101 24-25 0.7422462633068889 1101 26-27 0.7229436996238903 1101 28-29 0.6963311163389321 1101 30-31 0.6759176086490868 1101 32-33 0.6856932922263965 1101 34-35 0.7033096015325953 1101 36-37 0.6658248179353308 1101 38-39 0.6642015530724379 1101 40-41 0.6617920521851204 1101 42-43 0.6880957940127317 1101 44-45 0.7218249494751987 1101 46-47 0.7138867203006498 1101 48-49 0.7496044675514568 1101 50-51 0.6753556029604297 1101 52-53 0.7255093506163988 1101 54-55 0.7846834275548318 1101 56-57 0.7752851784506731 1101 58-59 0.6644307747939138 1101 60-61 0.6798085187865652 1101 62-63 0.6379420718763669 1101 64-65 0.6004709782032549 1101 66-67 0.5984186069692257 1101 68-69 0.634402291320157 1101 70-71 0.6182371247222918 1101 72-73 0.5871195836179481 1101 74-75 0.536567935247632 1101 76-77 0.49624109446001086 1101 78-79 0.5506655284940294 1101 80-81 0.49403422670851427 1101 82-83 0.5052906224834146 1101 84-85 0.4798746025466052 1101 86-87 0.4660415134810698 1101 88-89 0.4534981562038567 1101 90-91 0.46797511090612787 1101 92-93 0.4286766265068316 1101 94-95 0.47528933240903015 1101 96-97 0.47576108788019766 1101 98-99 0.4434264451735679 1101 100 0.41758128170980413 1102 1 0.45711622075948455 1102 2 0.49183652663850097 1102 3 0.5023287198540629 1102 4 0.4479980109224968 1102 5 0.44576046825036997 1102 6 0.4214698203419118 1102 7 0.42271434834919575 1102 8 0.4536913562901219 1102 9 0.5177833144850368 1102 10-11 0.5053485075521991 1102 12-13 0.5167981817092127 1102 14-15 0.6024258195370535 1102 16-17 0.5987700728430738 1102 18-19 0.5770451972081361 1102 20-21 0.5864673232331796 1102 22-23 0.6261394345964888 1102 24-25 0.5834032573006382 1102 26-27 0.5824900668520883 1102 28-29 0.6071389779655334 1102 30-31 0.5659141922708883 1102 32-33 0.5499871351591139 1102 34-35 0.5691387067350959 1102 36-37 0.5793504278274 1102 38-39 0.5996258442031532 1102 40-41 0.578573267774324 1102 42-43 0.5825124638654628 1102 44-45 0.574352843039442 1102 46-47 0.6173675621933867 1102 48-49 0.6320470370673164 1102 50-51 0.5910795812202494 1102 52-53 0.6155579299998024 1102 54-55 0.6510118643277778 1102 56-57 0.6323565942444631 1102 58-59 0.5248096180080992 1102 60-61 0.5412169383545091 1102 62-63 0.5342320214879877 1102 64-65 0.5112258632259667 1102 66-67 0.4964058140724248 1102 68-69 0.48856384442898104 1102 70-71 0.510692108343008 1102 72-73 0.4572620311646318 1102 74-75 0.44859068291514603 1102 76-77 0.45291424602881136 1102 78-79 0.40179024098880944 1102 80-81 0.3909052908981536 1102 82-83 0.36570991265297437 1102 84-85 0.33918711284295355 1102 86-87 0.3152848887421875 1102 88-89 0.3229590273470535 1102 90-91 0.30118992134879363 1102 92-93 0.2743976859268109 1102 94-95 0.3335587491884233 1102 96-97 0.33696694850402054 1102 98-99 0.2968171118520644 1102 100 0.26446990208663124 1103 1 0.41564710902301627 1103 2 0.43963117990904976 1103 3 0.4140215513152725 1103 4 0.3879618341999489 1103 5 0.3961838307268124 1103 6 0.3760288584658227 1103 7 0.366112703922127 1103 8 0.39665341978336244 1103 9 0.4694850234708241 1103 10-11 0.4571037427279947 1103 12-13 0.46078189620313736 1103 14-15 0.5371100093050032 1103 16-17 0.5267354382031115 1103 18-19 0.5005712390509984 1103 20-21 0.5292621502206174 1103 22-23 0.5732584387395931 1103 24-25 0.5841454925349794 1103 26-27 0.5486637674742028 1103 28-29 0.5445633101414558 1103 30-31 0.5336464656051874 1103 32-33 0.5043132291191199 1103 34-35 0.5145405080701408 1103 36-37 0.4915161103095258 1103 38-39 0.5258004366688454 1103 40-41 0.5114487636695699 1103 42-43 0.5393179675984285 1103 44-45 0.5261149869138677 1103 46-47 0.5858532154321026 1103 48-49 0.6461857825091144 1103 50-51 0.560647222223686 1103 52-53 0.5661231292478774 1103 54-55 0.6617500519483599 1103 56-57 0.7163098393371001 1103 58-59 0.6348496548173941 1103 60-61 0.6453204504197743 1103 62-63 0.5964053540545962 1103 64-65 0.6031393314304623 1103 66-67 0.5876417838382508 1103 68-69 0.5541303905280301 1103 70-71 0.5209914661429451 1103 72-73 0.49331445684313735 1103 74-75 0.5245980253764131 1103 76-77 0.5134361301714634 1103 78-79 0.5140286057632366 1103 80-81 0.4587958651696127 1103 82-83 0.4658747531533578 1103 84-85 0.45236438985230976 1103 86-87 0.39839813898340637 1103 88-89 0.4287525596297215 1103 90-91 0.441291612283802 1103 92-93 0.37639072996224243 1103 94-95 0.44053578925749903 1103 96-97 0.4294468214873852 1103 98-99 0.3949264143693334 1103 100 0.424419220367799 1104 1 0.36310677467956864 1104 2 0.4069640922556488 1104 3 0.3974804807158847 1104 4 0.34204062226258714 1104 5 0.3628929511216157 1104 6 0.30869723596062215 1104 7 0.333303269539833 1104 8 0.3836641347416929 1104 9 0.4326506471503322 1104 10-11 0.38561365819772675 1104 12-13 0.38491789727901704 1104 14-15 0.45348127194798593 1104 16-17 0.45241206838050374 1104 18-19 0.4180929471904449 1104 20-21 0.37070081409775213 1104 22-23 0.43403854449034185 1104 24-25 0.44831953112826994 1104 26-27 0.4157261087952264 1104 28-29 0.3962509165580599 1104 30-31 0.3662004148501481 1104 32-33 0.3658487277977258 1104 34-35 0.36758236018986423 1104 36-37 0.36216375531672185 1104 38-39 0.3326836931805204 1104 40-41 0.33070232372519826 1104 42-43 0.35487246789440974 1104 44-45 0.3737738126830763 1104 46-47 0.39166212630964026 1104 48-49 0.4270464127647884 1104 50-51 0.3785831825237196 1104 52-53 0.3569075980404861 1104 54-55 0.4183100571113201 1104 56-57 0.44937946301858744 1104 58-59 0.38504604504270645 1104 60-61 0.38935670313681925 1104 62-63 0.3616693034603955 1104 64-65 0.3604048804229265 1104 66-67 0.3710144121518866 1104 68-69 0.3267958681176708 1104 70-71 0.34639202930465274 1104 72-73 0.32751475839961586 1104 74-75 0.280909955724681 1104 76-77 0.2817148612640139 1104 78-79 0.2935036004695206 1104 80-81 0.2481990676079633 1104 82-83 0.24828903428198856 1104 84-85 0.230105662972111 1104 86-87 0.206478753889364 1104 88-89 0.20351728798046054 1104 90-91 0.2100696422782704 1104 92-93 0.23432913383253506 1104 94-95 0.3041764895984933 1104 96-97 0.22596273275857115 1104 98-99 0.22907094079269186 1104 100 0.18853092121010917 1105 1 0.31736542750665464 1105 2 0.3484810028825578 1105 3 0.3407690279702038 1105 4 0.2923204503460539 1105 5 0.29672268263899326 1105 6 0.2623257594016124 1105 7 0.30924491400148924 1105 8 0.2982106409172829 1105 9 0.3146951373733273 1105 10-11 0.3208384050292068 1105 12-13 0.3292631022118897 1105 14-15 0.37073722263343 1105 16-17 0.35570395210724826 1105 18-19 0.349942251947013 1105 20-21 0.373071104434743 1105 22-23 0.42388875007580396 1105 24-25 0.47379639281225394 1105 26-27 0.41876369724028706 1105 28-29 0.38231511150368647 1105 30-31 0.3750846464325619 1105 32-33 0.3728287909906598 1105 34-35 0.4059007141825788 1105 36-37 0.3957950084851731 1105 38-39 0.41128101592925503 1105 40-41 0.436564491687065 1105 42-43 0.42128799197917743 1105 44-45 0.4546001714930554 1105 46-47 0.46711321817784324 1105 48-49 0.4816441996588381 1105 50-51 0.38155377353231756 1105 52-53 0.39371190669453426 1105 54-55 0.49554957266459354 1105 56-57 0.501773741536141 1105 58-59 0.4146422680020905 1105 60-61 0.39008226031844373 1105 62-63 0.38092991737681103 1105 64-65 0.405818766989114 1105 66-67 0.3624160722535521 1105 68-69 0.35892557510758394 1105 70-71 0.3911060530173529 1105 72-73 0.35793497995513945 1105 74-75 0.3088300164942339 1105 76-77 0.3243008425097713 1105 78-79 0.34536050566821075 1105 80-81 0.3267875043694701 1105 82-83 0.2872883574806835 1105 84-85 0.26673420360181765 1105 86-87 0.25198023012476867 1105 88-89 0.2405002886164027 1105 90-91 0.2638313317224643 1105 92-93 0.2509484821228831 1105 94-95 0.32794041026186704 1105 96-97 0.28976097020527547 1105 98-99 0.27990056328634694 1105 100 0.2575494766774895 1106 1 0.22185141046893264 1106 2 0.2744140177698995 1106 3 0.26034220916447026 1106 4 0.24159801365463096 1106 5 0.2512373730031783 1106 6 0.2172419426651757 1106 7 0.22012521337372704 1106 8 0.24482716287636208 1106 9 0.2708491083024924 1106 10-11 0.2596642497903048 1106 12-13 0.25069035416262864 1106 14-15 0.31948584639415145 1106 16-17 0.2868333275211654 1106 18-19 0.24995958924671413 1106 20-21 0.2942890283815558 1106 22-23 0.2720657428105753 1106 24-25 0.25116378516842275 1106 26-27 0.294287165138293 1106 28-29 0.28156774807428064 1106 30-31 0.2767031059585179 1106 32-33 0.2882348984731635 1106 34-35 0.30456175467472235 1106 36-37 0.2726287719944125 1106 38-39 0.2762862005038045 1106 40-41 0.27443080164935196 1106 42-43 0.3038646396901399 1106 44-45 0.3289683088985669 1106 46-47 0.3105691364016252 1106 48-49 0.32996977843938424 1106 50-51 0.21571650812209242 1106 52-53 0.30550805382682 1106 54-55 0.4062871789786797 1106 56-57 0.4241200269559755 1106 58-59 0.3620500195537133 1106 60-61 0.36710734940723455 1106 62-63 0.36072713341718554 1106 64-65 0.31706630831683924 1106 66-67 0.33182903652333806 1106 68-69 0.32387063453780485 1106 70-71 0.35781534544349825 1106 72-73 0.3430167927100527 1106 74-75 0.35919627764598516 1106 76-77 0.3767957548099474 1106 78-79 0.3452205211644426 1106 80-81 0.291274836395111 1106 82-83 0.3495652979374171 1106 84-85 0.33398665087213786 1106 86-87 0.29361600822145917 1106 88-89 0.29894514940186667 1106 90-91 0.3147631125781416 1106 92-93 0.28472726617891553 1106 94-95 0.30308772480954715 1106 96-97 0.3378120795046762 1106 98-99 0.36144003403471814 1106 100 0.3309939986533763 1107 1 0.1797706704331894 1107 2 0.2089433819567148 1107 3 0.2023935753207624 1107 4 0.21806770088774385 1107 5 0.22321698965060222 1107 6 0.18492268287270974 1107 7 0.18383461075228524 1107 8 0.19552975685311225 1107 9 0.2121854037704196 1107 10-11 0.21420709567148322 1107 12-13 0.20688267841644148 1107 14-15 0.23773484261767663 1107 16-17 0.2276244834672312 1107 18-19 0.2166960752979179 1107 20-21 0.23422860591314532 1107 22-23 0.24961581534203248 1107 24-25 0.27754029404010794 1107 26-27 0.2508940251450227 1107 28-29 0.2730493975490589 1107 30-31 0.2500333887150177 1107 32-33 0.2035872567556254 1107 34-35 0.26054681185331674 1107 36-37 0.2313759052059723 1107 38-39 0.2592524267937293 1107 40-41 0.24668841347263992 1107 42-43 0.29339276921797364 1107 44-45 0.26579549625324006 1107 46-47 0.25646622855277457 1107 48-49 0.30016571216034293 1107 50-51 0.24658356552077265 1107 52-53 0.23158177715232142 1107 54-55 0.310165803279439 1107 56-57 0.32449673852284633 1107 58-59 0.295666908073251 1107 60-61 0.3162937092757687 1107 62-63 0.2908965416285767 1107 64-65 0.27715235540953387 1107 66-67 0.26167257290126145 1107 68-69 0.2606203365111597 1107 70-71 0.25022510010724375 1107 72-73 0.23639800572435377 1107 74-75 0.24117839804878827 1107 76-77 0.21105429662606667 1107 78-79 0.2310607022935045 1107 80-81 0.1675415773346316 1107 82-83 0.23621424507808797 1107 84-85 0.22240152419823644 1107 86-87 0.21310861490869115 1107 88-89 0.2204584870816646 1107 90-91 0.23461316567161106 1107 92-93 0.23617615727683017 1107 94-95 0.25756822479104713 1107 96-97 0.2835265944751164 1107 98-99 0.26639519846989757 1107 100 0.27902431187136045 1108 1 0.09900523824208918 1108 2 0.1258291948592074 1108 3 0.15211110983783271 1108 4 0.12858849879455647 1108 5 0.14750024046159638 1108 6 0.08908605606698217 1108 7 0.11436752711924214 1108 8 0.16054514002250642 1108 9 0.12293546553285495 1108 10-11 0.09074849136848684 1108 12-13 0.09303848165255602 1108 14-15 0.13664794581011108 1108 16-17 0.11967612412558282 1108 18-19 0.11480023646772963 1108 20-21 0.09988358912040951 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-1.1764352196857502 2316 94-95 -1.4213840944215796 2316 96-97 -1.5621669152695397 2316 98-99 -1.4346005342519668 2316 100 -1.2114272912541892 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18357.0 3 4575.0 4 3131.0 5 2686.0 6 3503.0 7 10929.0 8 21904.0 9 27154.0 10 30047.0 11 32377.0 12 37843.0 13 46206.0 14 56478.0 15 68137.0 16 81861.0 17 98339.0 18 113552.0 19 128952.0 20 149925.0 21 182956.0 22 234973.0 23 338140.0 24 523977.0 25 694605.0 26 691916.0 27 568613.0 28 488216.0 29 466942.0 30 510490.0 31 612674.0 32 760209.0 33 982024.0 34 1352230.0 35 2052081.0 36 3604233.0 37 7028808.0 38 9842248.0 39 3395631.0 40 5760.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 14.366566955125537 34.591119338558755 29.169299663429065 21.873014042886638 2 19.732256980745717 34.46063910583792 28.03679501905436 17.770308894361996 3 25.28180961121666 26.19698601657709 27.40968314599511 21.111521226211146 4 18.38310644300742 28.140215977740485 25.790405085357587 27.68627249389451 5 20.602735286215676 33.58946256643823 26.836430111745585 18.971372035600513 6 27.5517455705694 27.324142224803904 26.599080306414685 18.525031898212017 7 20.04712252680263 26.48361181594251 33.6560325243265 19.81323313292836 8 26.777536805797986 26.850294961968267 26.56986929367891 19.802298938554834 9 26.12998099444698 25.84899996428616 27.9897208875587 20.031298153708153 10-11 19.557434568416674 30.48440206586062 30.307003210851143 19.651160154871555 12-13 19.517603105940083 27.010696189962363 30.25946798979061 23.212232714306943 14-15 19.50666628642131 34.01332568811149 26.78963439990973 19.690373625557474 16-17 23.132709629005703 26.760766162294836 26.855937167517762 23.2505870411817 18-19 19.546762315576604 30.343650386719485 26.88736353267184 23.22222376503207 20-21 23.116164206027257 30.401081089137687 26.816806422089652 19.665948282745408 22-23 23.131655211987713 26.886108574350793 30.29314717138769 19.68908904227381 24-25 19.685274054367884 30.21354378515167 26.68586954234573 23.415312618134713 26-27 19.731511717386958 26.7112885353067 33.81368357750332 19.74351616980303 28-29 19.703277280543617 26.819087557935735 33.793883873427355 19.683751288093298 30-31 19.674766192074046 26.754452707905845 33.79690738788717 19.773873712132946 32-33 19.80946298894733 26.861697621514214 33.58518774026326 19.743651649275197 34-35 19.83994743244678 26.977250606906996 33.47772590274103 19.705076057905195 36-37 19.781318405399347 26.90687780738615 33.52274632400075 19.78905746321376 38-39 19.81286403065491 26.923098043700143 33.51975206741622 19.744285858228725 40-41 19.812844690465806 26.92690470914941 33.53653579700925 19.723714803375536 42-43 19.789932714684372 26.961635418332445 33.497331733448576 19.751100133534607 44-45 19.819209860503527 27.00911779290363 33.431093105615425 19.740579240977425 46-47 19.785643691966296 27.07636808978073 33.41243833971393 19.72554987853905 48-49 19.852937358220768 27.074909603081572 33.30843428283711 19.763718755860545 50-51 19.829073916587497 27.138580295446584 33.25545339092754 19.776892397038377 52-53 19.93786874327866 27.272119289606255 32.997152293750034 19.792859673365047 54-55 20.29923706758714 27.54799963587107 32.15757672339164 19.995186573150146 56-57 20.77626107622386 28.14965004152531 30.890213898791774 20.183874983459056 58-59 20.84143091213315 28.721978139119912 30.18675110285816 20.249839845888776 60-61 20.863970157833105 29.058336914710843 29.83392663157347 20.24376629588258 62-63 20.795412874384258 29.224760109931875 29.715100357528208 20.264726658155666 64-65 20.81749400852841 29.28824009705055 29.710845849711948 20.18342004470909 66-67 20.784697445053208 29.080931022670203 29.97934708668938 20.15502444558721 68-69 20.791422061282383 28.859298078042073 30.26162949841968 20.087650362255868 70-71 20.756321340053038 28.715009616306382 30.4927623565726 20.035906687067982 72-73 20.796704908082557 28.62891601792131 30.526996183332695 20.04738289066344 74-75 20.870520355654616 28.725805111447716 30.3792611865471 20.024413346350567 76-77 21.042619246203646 28.904835595053154 30.058994111771813 19.99355104697139 78-79 21.20377101186372 29.08670434787261 29.710279057210986 19.999245583052687 80-81 21.367117237481875 29.24565023516304 29.407422119422698 19.979810407932387 82-83 21.48347269403474 29.38418851826003 29.115847385060416 20.016491402644814 84-85 21.567033478066108 29.47593346224498 28.946284682802247 20.010748376886664 86-87 21.64858651786951 29.544271809161742 28.772497280454928 20.034644392513815 88-89 21.792704091054222 29.634417865837754 28.567569964987893 20.005308078120134 90-91 21.94401930609206 29.62744838948481 28.42440780150281 20.004124502920316 92-93 22.048337623704242 29.636536289133332 28.362522127104832 19.952603960057587 94-95 22.16079533436829 29.683815181914845 28.271150219275476 19.884239264441387 96-97 22.2230795354146 29.732098427616638 28.15654981743345 19.88827221953531 98-99 22.33321427590576 29.743878448162008 28.04311955818789 19.87978771774434 100 22.352156820824636 29.768365515220967 27.96424718089923 19.915230483055165 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 43340.0 1 31836.5 2 17251.0 3 13212.5 4 12096.0 5 11904.5 6 11959.0 7 11960.0 8 11753.5 9 11700.5 10 12459.5 11 15851.5 12 152398.0 13 193304.5 14 99230.0 15 105681.5 16 120098.0 17 133229.5 18 145501.0 19 157962.5 20 175045.0 21 204685.0 22 247000.0 23 291936.0 24 335226.5 25 382905.5 26 434468.5 27 497330.0 28 591022.0 29 679096.5 30 732567.5 31 790700.5 32 857127.5 33 898608.5 34 929490.0 35 991460.5 36 1058871.0 37 1086191.0 38 1106447.0 39 1120024.5 40 1116437.5 41 1136933.0 42 1189783.0 43 1190800.0 44 1149340.5 45 1131732.5 46 1115427.5 47 1104095.0 48 1072613.0 49 1029277.5 50 1006794.0 51 982298.5 52 948785.5 53 905783.0 54 841883.0 55 763212.0 56 682639.0 57 609138.5 58 538772.5 59 460330.5 60 382135.5 61 315008.0 62 249628.0 63 182518.5 64 127370.5 65 88195.0 66 61258.0 67 45113.5 68 33027.5 69 23463.5 70 17211.0 71 13168.5 72 10827.0 73 9132.0 74 8393.0 75 8396.5 76 6600.5 77 3493.0 78 1829.0 79 1389.5 80 1129.0 81 948.0 82 831.5 83 685.5 84 583.0 85 497.0 86 378.0 87 332.0 88 302.0 89 256.5 90 232.0 91 203.5 92 155.5 93 132.0 94 128.5 95 110.5 96 81.0 97 58.5 98 52.5 99 47.5 100 31.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.41493017174027197 2 0.392570091494602 3 0.1710814051508757 4 0.1773950730483154 5 0.28076118510069636 6 0.10314214269275017 7 0.13704656765255332 8 0.14320997762517748 9 0.13676306213403336 10-11 0.13317813485234836 12-13 0.13512581776458055 14-15 0.1412155163023895 16-17 0.145567326011671 18-19 0.15749582070339874 20-21 0.13876035851200655 22-23 0.09996971594051171 24-25 0.09501829205956043 26-27 0.08956222835564362 28-29 0.09933041099624916 30-31 0.10406920573831045 32-33 0.10521740308831633 34-35 0.1056299036177629 36-37 0.10159278503403851 38-39 0.10070824781625622 40-41 0.10179123889700249 42-43 0.06419415455847673 44-45 0.06716529239256601 46-47 0.0858610638113654 48-49 0.08990810508823797 50-51 0.09954162260754655 52-53 0.10476946436905478 54-55 0.10747552454332789 56-57 0.10814176251184983 58-59 0.1073635398635125 60-61 0.10538183628905791 62-63 0.10965568198074646 64-65 0.1166497631226341 66-67 0.12541717128286417 68-69 0.1307825132208546 70-71 0.12910557807880896 72-73 0.11557527720744343 74-75 0.1131314596378013 76-77 0.11512166837781147 78-79 0.11389975959299041 80-81 0.1082735925779616 82-83 0.1110547817146425 84-85 0.11713314003171066 86-87 0.13098521966659638 88-89 0.13894747215422973 90-91 0.14591462027185798 92-93 0.14508111404740925 94-95 0.13522787975124773 96-97 0.13140197277882074 98-99 0.12639951790453585 100 0.12067412395802507 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.5272682E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.714588645999726 #Duplication Level Percentage of deduplicated Percentage of total 1 74.95891517918635 26.021679057958387 2 11.734158341483944 8.146929598632829 3 4.341358393322216 4.521254123671171 4 2.105881241445422 2.924192041364202 5 1.3192730005864985 2.289900976356703 6 0.908297200323799 1.8918698206532334 7 0.6552531595547166 1.592279072505353 8 0.4884843135708495 1.3566025604508465 9 0.3935783723506976 1.2296620166504921 >10 2.591427753248256 17.212030723349248 >50 0.27434833344089427 6.6336287461316905 >100 0.21069948658244492 15.361660968702987 >500 0.017467613661530164 3.8286113744158468 >1k 7.759469426053823E-4 0.5250745174871627 >5k 4.899857991624136E-5 0.11220135712082481 >10k+ 3.266571969366331E-5 6.35242304454913 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2151692 6.100165561552705 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 49135 0.13930043652478707 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0034219116085360338 0.0 0.0 2.8350551851996966E-6 0.0 2 0.0035324787607588217 1.1340220740798787E-5 0.0 2.8350551851996966E-6 5.670110370399393E-6 3 0.003708252182241203 1.4175275925998483E-5 0.0 5.670110370399393E-6 1.1340220740798787E-5 4 0.003725262513352401 2.2680441481597573E-5 0.0 5.670110370399393E-6 1.4175275925998483E-5 5 0.003725262513352401 3.4020662222396354E-5 0.0 5.670110370399393E-6 1.4175275925998483E-5 6 0.003725262513352401 3.969077259279575E-5 0.0 5.670110370399393E-6 1.4175275925998483E-5 7 0.0037309326237228006 4.2525827777995445E-5 0.0 1.4175275925998483E-5 1.4175275925998483E-5 8 0.003745107899648799 4.5360882963195146E-5 0.0 2.2680441481597573E-5 1.4175275925998483E-5 9 0.003756448120389598 4.819593814839484E-5 0.0 2.5515496666797268E-5 1.4175275925998483E-5 10-11 0.0037592831755747973 5.670110370399393E-5 0.0 2.5515496666797268E-5 1.9845386296397875E-5 12-13 0.003760700703167397 6.520626925959301E-5 0.0 2.5515496666797268E-5 1.9845386296397875E-5 14-15 0.003762118230759997 7.087637962999242E-5 0.0 2.8350551851996965E-5 2.409796907419742E-5 16-17 0.003764953285945197 7.229390722259226E-5 0.0 3.118560703719666E-5 2.5515496666797268E-5 18-19 0.0037692058687229966 7.65464900003918E-5 0.0 3.118560703719666E-5 3.260313462979651E-5 20-21 0.0037777110342785956 8.788671074119058E-5 0.0 3.118560703719666E-5 3.4020662222396354E-5 22-23 0.003791886310204594 1.0206198666718907E-4 0.0 3.6855717407596056E-5 3.6855717407596056E-5 24-25 0.003842917303538189 1.0631456944498861E-4 0.0 4.11083001853956E-5 4.11083001853956E-5 26-27 0.004123587766872959 1.1340220740798786E-4 0.0 4.2525827777995445E-5 4.2525827777995445E-5 28-29 0.004920238273914073 1.2190737296358695E-4 0.0 4.5360882963195146E-5 4.677841055579499E-5 30-31 0.007043694607628646 1.3041253851918603E-4 0.0 4.819593814839484E-5 5.5283576111394084E-5 32-33 0.011540092131355364 1.3891770407478514E-4 0.0 5.1030993333594535E-5 6.378874166699317E-5 34-35 0.020189845501399635 1.4458781444518453E-4 0.0 5.5283576111394084E-5 6.945885203739257E-5 36-37 0.034413317365546515 1.4742286963038422E-4 0.0 6.095368648179347E-5 7.371143481519211E-5 38-39 0.05476334348490994 1.5025792481558392E-4 0.0 6.520626925959301E-5 8.079907277819135E-5 40-41 0.08235268301968078 1.5167545240818376E-4 0.0 6.662379685219286E-5 8.788671074119058E-5 42-43 0.11829409513004993 1.615981455563827E-4 0.0 6.804132444479271E-5 9.213929351899014E-5 44-45 0.1633757251575029 1.6726825592678208E-4 0.0 6.804132444479271E-5 9.497434870418983E-5 46-47 0.21974796246001366 1.7719094907498102E-4 0.0 7.229390722259226E-5 9.922693148198938E-5 48-49 0.28811815330628954 1.8569611463058013E-4 0.0 7.371143481519211E-5 1.0064445907458922E-4 50-51 0.36836297279577435 1.8711364222317997E-4 0.0 7.371143481519211E-5 1.0206198666718907E-4 52-53 0.4601337091406885 1.9136622500097951E-4 0.0 7.65464900003918E-5 1.0489704185238876E-4 54-55 0.5655127103745612 2.140466664825771E-4 0.0 8.22166003707912E-5 1.1056715222278817E-4 56-57 0.6852909568940633 2.2680441481597572E-4 0.0 9.213929351899014E-5 1.1481973500058771E-4 58-59 0.8219122662688366 2.3672710796417465E-4 0.0 9.355682111158999E-5 1.2190737296358695E-4 60-61 0.9726960938212751 2.438147459271739E-4 0.0 9.355682111158999E-5 1.2899501092658618E-4 62-63 1.1393363850245355 2.565724942605725E-4 0.0 9.497434870418983E-5 1.4742286963038422E-4 64-65 1.3201292717123128 2.6933024259397114E-4 0.0 9.922693148198938E-5 1.530929800007836E-4 66-67 1.5180685721601777 2.778354081495703E-4 0.0 1.0206198666718907E-4 1.58763090371183E-4 68-69 1.7333130494585016 2.8634057370516936E-4 0.0 1.0347951425978892E-4 1.6726825592678208E-4 70-71 1.9666664417522886 2.962632668533683E-4 0.0 1.0773209703758846E-4 1.7010331111198178E-4 72-73 2.216484417034123 3.0760348759416707E-4 0.0 1.0773209703758846E-4 1.7010331111198178E-4 74-75 2.483406280248267 3.118560703719666E-4 0.0 1.1056715222278817E-4 1.7293836629718147E-4 76-77 2.769342858589545 3.2744887389056493E-4 0.0 1.1481973500058771E-4 1.7293836629718147E-4 78-79 3.074031909453327 3.317014566683645E-4 0.0 1.1907231777838725E-4 1.7577342148238117E-4 80-81 3.3997684100120313 3.387890946313637E-4 0.0 1.1907231777838725E-4 1.7577342148238117E-4 82-83 3.7444827699804626 3.4871178777956264E-4 0.0 1.1907231777838725E-4 1.7860847666758086E-4 84-85 4.109109140042143 3.586344809277616E-4 0.0 1.2474242814878664E-4 1.8853116981577982E-4 86-87 4.494530639887264 3.6288706370556117E-4 0.0 1.261599557413865E-4 1.9136622500097951E-4 88 4.796213114727142 3.6572211889076086E-4 0.0 1.3041253851918603E-4 1.9278375259357936E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 266820 0.0 87.08304 8 GTGGTAT 269325 0.0 86.27249 6 TGGTATC 270485 0.0 85.66109 7 AGTGGTA 271610 0.0 85.56076 5 GTATCAA 272080 0.0 85.37106 9 GCAGTGG 279765 0.0 83.6336 3 AAGCAGT 281750 0.0 83.0045 1 CAGTGGT 286780 0.0 81.001884 4 AGCAGTG 293570 0.0 79.72281 2 CAACGCA 263790 0.0 45.45114 12-13 ATCAACG 265150 0.0 45.273464 10-11 ACGCAGA 265950 0.0 45.08553 14-15 TCAACGC 263225 0.0 44.350384 12-13 AGAGTAC 266385 0.0 44.333694 18-19 AACGCAG 265070 0.0 44.11517 14-15 CGCAGAG 266670 0.0 43.768578 16-17 GAGTACT 262950 0.0 43.467075 20-21 TATCAAC 270180 0.0 42.94491 10-11 AGTACTT 277225 0.0 42.308952 20-21 CAGAGTA 278930 0.0 41.40177 18-19 >>END_MODULE