FastQCFastQC Report
Thu 26 May 2016
SRR1660717_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660717_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35272682
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21218166.015465452839679No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT524270.1486334381944645No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT456670.12946846514251453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2738800.086.672358
GTGGTAT2739650.085.791896
TGGTATC2783200.085.2715157
AGTGGTA2759700.085.189365
GTATCAA2785050.085.16389
GCAGTGG2763850.085.0384753
AAGCAGT2716900.084.301131
CAGTGGT2905450.080.858414
AGCAGTG2900400.080.7921752
CAACGCA2692800.045.31059312-13
ATCAACG2705200.045.1697410-11
ACGCAGA2721850.044.89259314-15
AGAGTAC2753400.044.27865218-19
TCAACGC2686900.044.15141712-13
AACGCAG2703750.043.90844314-15
CGCAGAG2733950.043.43292216-17
GAGTACT2716200.043.36525720-21
TATCAAC2763900.042.88490710-11
AGTACTT2870150.042.23230420-21
GTACTTT2857600.041.2347322-23
Produced by FastQC (version 0.11.5)