FastQCFastQC Report
Thu 26 May 2016
SRR1660716_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660716_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43049396
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27516346.391806286898892No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA725970.16863651234502802No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT493080.11453819235930744No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3506050.086.7934958
AGTGGTA3536150.086.18965
GTGGTAT3544000.085.953646
TGGTATC3538700.085.794287
GTATCAA3567200.085.2119
AAGCAGT3603400.085.035561
GCAGTGG3632950.084.401113
CAGTGGT3722700.081.818644
AGCAGTG3772250.081.308692
CAACGCA3492350.044.85070812-13
ATCAACG3500300.044.79652410-11
AGAGTAC3408900.044.3796418-19
ACGCAGA3533100.044.3736514-15
TCAACGC3479300.043.87795612-13
GAGTACT3346850.043.67193220-21
AACGCAG3514250.043.52873214-15
CGCAGAG3552400.043.0227816-17
TATCAAC3552550.042.75573710-11
AGTACTT3506800.042.5271320-21
GTACTTT3478550.041.92508722-23
Produced by FastQC (version 0.11.5)