##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660716_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 43049396 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.936478574519374 34.0 31.0 34.0 30.0 34.0 2 32.0615608869402 34.0 31.0 34.0 30.0 34.0 3 32.12153246006053 34.0 31.0 34.0 30.0 34.0 4 35.52838497432113 37.0 35.0 37.0 33.0 37.0 5 35.49327279760208 37.0 35.0 37.0 33.0 37.0 6 35.570082632518236 37.0 35.0 37.0 33.0 37.0 7 35.51142750063207 37.0 35.0 37.0 33.0 37.0 8 35.533775293850816 37.0 35.0 37.0 33.0 37.0 9 37.2289611914648 39.0 37.0 39.0 34.0 39.0 10-11 37.14790726680579 39.0 37.0 39.0 34.0 39.0 12-13 37.19170726576512 39.0 37.0 39.0 34.0 39.0 14-15 38.58719063096727 40.0 38.0 41.0 34.0 41.0 16-17 38.562603479965205 40.0 38.0 41.0 34.0 41.0 18-19 38.5168956725897 40.0 38.0 41.0 34.0 41.0 20-21 38.38303880035855 40.0 38.0 41.0 34.0 41.0 22-23 38.18138389212243 40.0 38.0 41.0 33.5 41.0 24-25 38.17665285013523 40.0 38.0 41.0 33.0 41.0 26-27 38.174913255461234 40.0 38.0 41.0 33.0 41.0 28-29 38.096911173852476 40.0 38.0 41.0 33.0 41.0 30-31 38.001484306539396 40.0 38.0 41.0 33.0 41.0 32-33 37.866474735208826 40.0 38.0 41.0 32.5 41.0 34-35 37.69679665192051 40.0 38.0 41.0 32.0 41.0 36-37 37.48362605366171 40.0 37.0 41.0 31.5 41.0 38-39 37.00133127303343 40.0 35.0 41.0 31.0 41.0 40-41 36.93046255515408 39.5 35.0 41.0 31.0 41.0 42-43 36.92598627864605 40.0 35.0 41.0 31.0 41.0 44-45 36.9162714617413 40.0 35.0 41.0 31.0 41.0 46-47 36.99437722657015 40.0 35.0 41.0 31.0 41.0 48-49 36.88101823774717 40.0 35.0 41.0 30.5 41.0 50-51 35.50969364355309 38.5 34.0 40.0 28.0 40.5 52-53 35.65094787857186 38.5 35.0 39.5 28.0 40.5 54-55 35.99518799288148 39.0 35.0 41.0 27.0 41.0 56-57 35.58953405525132 39.0 35.0 41.0 23.5 41.0 58-59 35.38441087535816 39.0 35.0 41.0 23.0 41.0 60-61 35.154895030350716 39.0 35.0 41.0 22.0 41.0 62-63 34.92029862625715 38.5 35.0 41.0 22.0 41.0 64-65 34.664573435594775 38.0 34.0 40.5 22.0 41.0 66-67 34.39617748643907 37.0 34.0 40.0 22.0 41.0 68-69 34.11909608441428 37.0 34.0 40.0 21.5 41.0 70-71 33.81595306238443 36.0 34.0 39.0 21.0 41.0 72-73 33.464327292768516 36.0 33.0 39.0 20.0 41.0 74-75 33.07381601823171 35.0 33.0 39.0 20.0 40.5 76-77 32.66914298867283 35.0 33.0 37.0 19.0 39.5 78-79 32.28360439714416 35.0 32.5 37.0 18.0 39.0 80-81 31.94675445620654 35.0 32.0 36.5 17.5 39.0 82-83 31.617710548134056 35.0 32.0 36.0 16.0 38.0 84-85 31.315951761553173 35.0 32.0 36.0 15.0 37.0 86-87 31.083267753628878 35.0 31.5 35.0 15.0 37.0 88-89 30.865861358426493 35.0 31.0 35.0 14.0 36.0 90-91 30.682764062473723 35.0 31.0 35.0 12.0 36.0 92-93 30.522282565822756 35.0 31.0 35.0 10.5 36.0 94-95 30.335140439601055 35.0 31.0 35.0 10.0 36.0 96-97 30.195755139050036 35.0 30.5 35.0 10.0 35.0 98-99 30.090114365367633 34.5 30.0 35.0 10.0 35.0 100 30.006429312039593 34.0 30.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5057670671478256 1101 2 0.5360220478301514 1101 3 0.5385879139145686 1101 4 0.4441945748088756 1101 5 0.4444227335481088 1101 6 0.428402709741853 1101 7 0.45444390233866727 1101 8 0.46413441351799634 1101 9 0.5233510966867811 1101 10-11 0.5525647856663412 1101 12-13 0.5487895400328 1101 14-15 0.6807921276016486 1101 16-17 0.6462288648042076 1101 18-19 0.6272815829757192 1101 20-21 0.674855769798441 1101 22-23 0.7286655553691901 1101 24-25 0.7319328945407406 1101 26-27 0.7144022220334705 1101 28-29 0.6872376397579458 1101 30-31 0.6540517004562219 1101 32-33 0.6834615208038954 1101 34-35 0.728309292680251 1101 36-37 0.6800077711762853 1101 38-39 0.678120558690388 1101 40-41 0.6628953022364144 1101 42-43 0.6930572146509419 1101 44-45 0.7170469251680416 1101 46-47 0.7271706969261373 1101 48-49 0.7790024962063526 1101 50-51 0.7182212326841295 1101 52-53 0.7755080083756738 1101 54-55 0.7939447084972713 1101 56-57 0.8021535164346076 1101 58-59 0.7441867632781936 1101 60-61 0.7324417144296191 1101 62-63 0.6724541061796216 1101 64-65 0.635644571380567 1101 66-67 0.6290463803003874 1101 68-69 0.602071888763831 1101 70-71 0.5749531890236312 1101 72-73 0.5634937848056083 1101 74-75 0.5288088020348383 1101 76-77 0.5289610076949529 1101 78-79 0.5113835262379212 1101 80-81 0.45045142125776394 1101 82-83 0.46357556680855794 1101 84-85 0.4600229462051786 1101 86-87 0.42552446262424226 1101 88-89 0.4209121651839389 1101 90-91 0.4264595083164515 1101 92-93 0.4368335579613962 1101 94-95 0.4418197876486367 1101 96-97 0.4301860185282216 1101 98-99 0.4199216435568722 1101 100 0.42603575396489646 1102 1 0.4233893587944486 1102 2 0.45751610504994744 1102 3 0.4605631828620034 1102 4 0.39087692702540977 1102 5 0.394493641172339 1102 6 0.36100400084092144 1102 7 0.37447007332475124 1102 8 0.3936885592230652 1102 9 0.46649833939349605 1102 10-11 0.47368142250212486 1102 12-13 0.48902362049629744 1102 14-15 0.571069500402217 1102 16-17 0.5352974951001386 1102 18-19 0.5137797849885075 1102 20-21 0.5332868303051654 1102 22-23 0.5803358150487412 1102 24-25 0.529795276328656 1102 26-27 0.5374288058337413 1102 28-29 0.5517824750743827 1102 30-31 0.5356575595716819 1102 32-33 0.5520013017705665 1102 34-35 0.5440367623382372 1102 36-37 0.5667212204161061 1102 38-39 0.5893327383586637 1102 40-41 0.5775241249303988 1102 42-43 0.5614387289058911 1102 44-45 0.5724801962400008 1102 46-47 0.5904273006392131 1102 48-49 0.6424380475482181 1102 50-51 0.597809480453158 1102 52-53 0.6283676154738842 1102 54-55 0.6716830597435361 1102 56-57 0.712096064395574 1102 58-59 0.5948496817344093 1102 60-61 0.5997128853286782 1102 62-63 0.6004333445956149 1102 64-65 0.5815763551065558 1102 66-67 0.5563590444043527 1102 68-69 0.5353116732831467 1102 70-71 0.5299197279246073 1102 72-73 0.49745414106899233 1102 74-75 0.49832548713445846 1102 76-77 0.4864062892558394 1102 78-79 0.4932111000753565 1102 80-81 0.4663218342278235 1102 82-83 0.4645588935201914 1102 84-85 0.42600866104257307 1102 86-87 0.3911097279587068 1102 88-89 0.3705361076837157 1102 90-91 0.3597002629076904 1102 92-93 0.358185143153694 1102 94-95 0.39663506894387623 1102 96-97 0.38123469139240385 1102 98-99 0.33631514815793295 1102 100 0.3108104154338598 1103 1 0.32499358220135477 1103 2 0.3701063390590491 1103 3 0.37748822240663316 1103 4 0.30423381523814186 1103 5 0.32765854485006685 1103 6 0.2915294579422536 1103 7 0.2991061998211322 1103 8 0.3140220049967155 1103 9 0.35539211456503494 1103 10-11 0.3483823273209552 1103 12-13 0.35803910118191595 1103 14-15 0.4226084475013536 1103 16-17 0.4007581895266128 1103 18-19 0.40247282489663405 1103 20-21 0.4112811728917478 1103 22-23 0.45831267846868684 1103 24-25 0.5098700560890705 1103 26-27 0.4597659431773735 1103 28-29 0.443462533703773 1103 30-31 0.41144881391664256 1103 32-33 0.36173536782589366 1103 34-35 0.3808104898317026 1103 36-37 0.39648847822490296 1103 38-39 0.41306303344708084 1103 40-41 0.3800335211150454 1103 42-43 0.41091668937213655 1103 44-45 0.43309757451108766 1103 46-47 0.4651902410049118 1103 48-49 0.49477299920391005 1103 50-51 0.44018607296463585 1103 52-53 0.41259997344303656 1103 54-55 0.4340849012572576 1103 56-57 0.48354056475352536 1103 58-59 0.4648401262389257 1103 60-61 0.45060460472064534 1103 62-63 0.4202201792517215 1103 64-65 0.39386249691895614 1103 66-67 0.4144497072047315 1103 68-69 0.3876127526262749 1103 70-71 0.3785642668417779 1103 72-73 0.33985943218198855 1103 74-75 0.3684902268487704 1103 76-77 0.3286178667192985 1103 78-79 0.34964279012744726 1103 80-81 0.2771730741044074 1103 82-83 0.2924627923282159 1103 84-85 0.266273114510998 1103 86-87 0.22952289963000894 1103 88-89 0.24498968708880753 1103 90-91 0.21690523689263586 1103 92-93 0.20787057040530854 1103 94-95 0.28337387777888523 1103 96-97 0.24390192197828853 1103 98-99 0.25081904518941656 1103 100 0.23816279233732018 1104 1 0.38260929648508935 1104 2 0.3861716733078069 1104 3 0.36433697122357955 1104 4 0.3023296671354103 1104 5 0.33390411749506654 1104 6 0.26428115572525 1104 7 0.2797064671641607 1104 8 0.30045229333872214 1104 9 0.36320196669916527 1104 10-11 0.3628222568889967 1104 12-13 0.35380344257197294 1104 14-15 0.42982683819152356 1104 16-17 0.41724900000648546 1104 18-19 0.40025993786729686 1104 20-21 0.34970577546719284 1104 22-23 0.40340324729609733 1104 24-25 0.4214430476642761 1104 26-27 0.3856982343224331 1104 28-29 0.378767874466007 1104 30-31 0.3490503633514592 1104 32-33 0.35474402623298573 1104 34-35 0.35343222166066823 1104 36-37 0.35544491206034934 1104 38-39 0.34563337978283926 1104 40-41 0.3385448643425306 1104 42-43 0.3346186992127329 1104 44-45 0.339729208625009 1104 46-47 0.40380514229835995 1104 48-49 0.42732820201982236 1104 50-51 0.35097935229778443 1104 52-53 0.3684398195008214 1104 54-55 0.4661632349658973 1104 56-57 0.5111412089816483 1104 58-59 0.47016141980155624 1104 60-61 0.45578214604550027 1104 62-63 0.42394600037447105 1104 64-65 0.39577185482557553 1104 66-67 0.39549984646172476 1104 68-69 0.37208345423883316 1104 70-71 0.34019052429199803 1104 72-73 0.3218541347114794 1104 74-75 0.32825818416621644 1104 76-77 0.3375197917097523 1104 78-79 0.3504058829124759 1104 80-81 0.28740411998476745 1104 82-83 0.2912628977932208 1104 84-85 0.2860007993660858 1104 86-87 0.24980123362457007 1104 88-89 0.2619528307290331 1104 90-91 0.2603260102230891 1104 92-93 0.3022606050527763 1104 94-95 0.35174094395324573 1104 96-97 0.3122997506489007 1104 98-99 0.3111590799323629 1104 100 0.22735068570686323 1105 1 0.2480897452506845 1105 2 0.3062837152085791 1105 3 0.28724437698906513 1105 4 0.2512516168221097 1105 5 0.2599111940589225 1105 6 0.24626678300424487 1105 7 0.2699945939501447 1105 8 0.2623627443554284 1105 9 0.2691286424952821 1105 10-11 0.2810441530393959 1105 12-13 0.27287855543738715 1105 14-15 0.3203428244300852 1105 16-17 0.305156769748713 1105 18-19 0.3098652666777255 1105 20-21 0.34828030766317397 1105 22-23 0.3648667674004713 1105 24-25 0.37784476948495893 1105 26-27 0.387175014801997 1105 28-29 0.3617263158013273 1105 30-31 0.3675881343011085 1105 32-33 0.3436683256860036 1105 34-35 0.3585880377788584 1105 36-37 0.3453879362021084 1105 38-39 0.3688732888942212 1105 40-41 0.36226759513920115 1105 42-43 0.37684442928570405 1105 44-45 0.4016555337144112 1105 46-47 0.41144368454492763 1105 48-49 0.4477239733798015 1105 50-51 0.3254507841106147 1105 52-53 0.3850888258208158 1105 54-55 0.4615018392534367 1105 56-57 0.47492968849582695 1105 58-59 0.43693585600426843 1105 60-61 0.41278526268400384 1105 62-63 0.39700023947586516 1105 64-65 0.4149849050421821 1105 66-67 0.4079141805201587 1105 68-69 0.4063346636334799 1105 70-71 0.38056989858999657 1105 72-73 0.38551250334769094 1105 74-75 0.4055928455205944 1105 76-77 0.37415018358333896 1105 78-79 0.37414207596304294 1105 80-81 0.3392148854540089 1105 82-83 0.3178968419142194 1105 84-85 0.32606494877517633 1105 86-87 0.2787626355390991 1105 88-89 0.28714128875404654 1105 90-91 0.29898364366964003 1105 92-93 0.2758820856195321 1105 94-95 0.33473502261117005 1105 96-97 0.3150381328762215 1105 98-99 0.2821503544303532 1105 100 0.28205344696463897 1106 1 0.23350820173747522 1106 2 0.28312248603705825 1106 3 0.2706361410514191 1106 4 0.24842808899696678 1106 5 0.25152453215243753 1106 6 0.23783504251616705 1106 7 0.2353721664836428 1106 8 0.2382611627135205 1106 9 0.23776141274099416 1106 10-11 0.23289904189494592 1106 12-13 0.2651311066784672 1106 14-15 0.3303707268498286 1106 16-17 0.309715390513162 1106 18-19 0.2653121504306952 1106 20-21 0.2975515862892806 1106 22-23 0.2741601468577244 1106 24-25 0.2874029415870112 1106 26-27 0.31012774828297296 1106 28-29 0.3164458801532959 1106 30-31 0.292022095903711 1106 32-33 0.28992866943111295 1106 34-35 0.2985259628525512 1106 36-37 0.263610940380282 1106 38-39 0.2708364476569116 1106 40-41 0.3194770623084011 1106 42-43 0.3247738795031978 1106 44-45 0.34516203136416124 1106 46-47 0.3332888413158983 1106 48-49 0.34680943647672535 1106 50-51 0.26014874098916607 1106 52-53 0.30490621810090346 1106 54-55 0.4565940640547268 1106 56-57 0.43146448922814074 1106 58-59 0.3503010059910707 1106 60-61 0.3561826089497515 1106 62-63 0.366760228035524 1106 64-65 0.35103343518056107 1106 66-67 0.36737128420492837 1106 68-69 0.37832865482014455 1106 70-71 0.39145349648394756 1106 72-73 0.3718024514568796 1106 74-75 0.36882692476668666 1106 76-77 0.35571507131679425 1106 78-79 0.36948657043000566 1106 80-81 0.3331175644838531 1106 82-83 0.354027529631999 1106 84-85 0.34254231233030197 1106 86-87 0.31350898508291536 1106 88-89 0.32567605441373715 1106 90-91 0.3249981362678902 1106 92-93 0.2985253357860955 1106 94-95 0.3667423511331336 1106 96-97 0.39443563059854725 1106 98-99 0.3535652220529819 1106 100 0.3282174673517986 1107 1 0.2017635978785428 1107 2 0.22257911016448162 1107 3 0.21917367343667848 1107 4 0.19731053101332208 1107 5 0.22061565229240898 1107 6 0.1829998139736091 1107 7 0.18241317727839146 1107 8 0.19711777059902147 1107 9 0.22467773174840033 1107 10-11 0.20153929348223443 1107 12-13 0.19761038258913288 1107 14-15 0.2305526360560961 1107 16-17 0.22524894686543462 1107 18-19 0.19681500562188603 1107 20-21 0.2262189346583483 1107 22-23 0.2665432191822461 1107 24-25 0.27888875013396586 1107 26-27 0.2563976822630991 1107 28-29 0.2502341009757316 1107 30-31 0.22593487993898265 1107 32-33 0.1961906038057819 1107 34-35 0.23360906686237115 1107 36-37 0.2263274703257352 1107 38-39 0.24926047388053263 1107 40-41 0.27053748314730797 1107 42-43 0.29821266826896675 1107 44-45 0.31002869148431245 1107 46-47 0.30571902209284474 1107 48-49 0.34180725269313683 1107 50-51 0.2615418865172643 1107 52-53 0.2850977376662627 1107 54-55 0.4018750660608461 1107 56-57 0.4527457991039583 1107 58-59 0.3646912330451073 1107 60-61 0.39519946793621585 1107 62-63 0.38925993699979955 1107 64-65 0.3661763804623064 1107 66-67 0.38941962970546484 1107 68-69 0.3620693486090758 1107 70-71 0.34904269157455303 1107 72-73 0.31576585313069216 1107 74-75 0.3321593293591505 1107 76-77 0.34730521207170284 1107 78-79 0.34609654260771805 1107 80-81 0.29866149435506983 1107 82-83 0.34035824196595144 1107 84-85 0.3351873776903176 1107 86-87 0.31349802887826783 1107 88-89 0.3285116051501227 1107 90-91 0.3070187641197819 1107 92-93 0.30044946753434587 1107 94-95 0.34870700500251317 1107 96-97 0.37968795463998006 1107 98-99 0.39562611622407573 1107 100 0.3663239036381398 1108 1 0.10630383388179965 1108 2 0.13227022783141962 1108 3 0.18323313001369712 1108 4 0.14775984856515834 1108 5 0.13997192525617663 1108 6 0.09833697098900274 1108 7 0.1306257707840146 1108 8 0.14852939394551612 1108 9 0.14980486755226963 1108 10-11 0.11167907993183945 1108 12-13 0.11580233181571487 1108 14-15 0.16298900741403344 1108 16-17 0.1238279325094922 1108 18-19 0.12409975019011199 1108 20-21 0.1154045934876251 1108 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-1.1398655361481467 2316 82-83 -1.0682570065708887 2316 84-85 -1.1850810024992313 2316 86-87 -1.3458490225315778 2316 88-89 -1.3123206978795814 2316 90-91 -1.2432242763057744 2316 92-93 -1.1133181316281586 2316 94-95 -1.3052027703893678 2316 96-97 -1.448953814016928 2316 98-99 -1.3040359372269528 2316 100 -1.139889051265218 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22262.0 3 5563.0 4 3891.0 5 3284.0 6 4766.0 7 20793.0 8 42677.0 9 51421.0 10 54335.0 11 52516.0 12 54910.0 13 59452.0 14 72113.0 15 98622.0 16 132807.0 17 171982.0 18 180049.0 19 185460.0 20 205815.0 21 250905.0 22 335983.0 23 497451.0 24 710041.0 25 854655.0 26 828660.0 27 693732.0 28 613562.0 29 600830.0 30 659582.0 31 788449.0 32 973716.0 33 1253957.0 34 1719588.0 35 2600208.0 36 4516469.0 37 8576380.0 38 1.1467304E7 39 3678349.0 40 6857.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 14.533657433599915 34.61744991143949 28.905316823062016 21.943575831898567 2 19.692973444787125 35.49526834859375 26.992328859898773 17.819429346720362 3 26.028096300042336 25.984966014889626 26.885112936149074 21.10182474891896 4 18.471906980077733 28.20013812996783 24.933997331410932 28.39395755854351 5 20.76778958867024 34.14032307386412 26.239455776735188 18.85243156073046 6 28.2871550926778 27.35067707254124 25.980945454636778 18.381222380144184 7 20.134569045007133 26.37850043866445 33.601561769646146 19.88536874668228 8 27.602441896759533 26.98058319322907 25.65048631227409 19.766488597737304 9 26.785705521422447 26.0329945764236 27.174745042596705 20.006554859557248 10-11 19.626786556022484 30.960317187198765 29.8158311434083 19.59706511337045 12-13 19.502216239043406 27.19337558643233 29.848431344424537 23.455976830099722 14-15 19.480684895886068 34.770157933358114 26.117476623925523 19.631680546830292 16-17 23.361854610726734 26.965199820174647 26.21308634823894 23.459859220859684 18-19 19.479149214047144 30.859270928622962 26.235836819378665 23.42574303795123 20-21 23.302782240342644 30.983808680957576 26.164938777807333 19.548470300892447 22-23 23.298660839644945 27.223512284225354 29.900094895543344 19.577731980586353 24-25 19.575453733357453 30.91973863916962 26.007644027702277 23.49716359977065 26-27 19.6008651162343 27.216522646863776 33.54969957870642 19.632912658195504 28-29 19.613166901146233 27.32392957318952 33.51379488227082 19.549108643393424 30-31 19.554653491005507 27.231211960501618 33.52863480401518 19.685499744477696 32-33 19.691410735173044 27.334959177102736 33.31362301219253 19.660007075531695 34-35 19.7237540591301 27.409955025880212 33.21670969183352 19.649581223156165 36-37 19.67303936802446 27.324020002281074 33.30020198299799 19.702738646696474 38-39 19.722728979401214 27.295503641065586 33.30719530087425 19.674572078658944 40-41 19.754452940985594 27.275806129208014 33.31487192644577 19.654869003360616 42-43 19.75848100798003 27.319933896737798 33.2575149052815 19.664070190000682 44-45 19.78823631551063 27.3893781576635 33.197679287122114 19.624706239703755 46-47 19.786455968301595 27.47324088194881 33.13883062421337 19.601472525536227 48-49 19.85761397623206 27.47116822285518 33.01935020613233 19.651867594780427 50-51 19.875597818892725 27.52908058167232 32.9323405142558 19.662981085179158 52-53 20.026715687670844 27.635211690258473 32.66597054112347 19.672102080947205 54-55 20.429890578626043 27.873700482823978 31.88603650144788 19.810372437102103 56-57 20.984838104229638 28.422994829764765 30.667091439651955 19.925075626353635 58-59 21.197795619890897 28.971730447408156 29.908678381967114 19.921795550733837 60-61 21.433352072060607 29.250525436028862 29.4613125738418 19.85480991806873 62-63 21.564147038700625 29.41068761771585 29.207743155093585 19.817422188489935 64-65 21.787073925208713 29.470087162012113 29.057431978232533 19.68540693454664 66-67 21.973295471830358 29.283438758634407 29.142075621725215 19.601190147810016 68-69 22.162403391158687 29.078738921905728 29.27103873964341 19.48781894729218 70-71 22.339382523983524 28.906418335437788 29.361221729880317 19.39297741069837 72-73 22.581726518061 28.788680848396837 29.304186152839268 19.3254064807029 74-75 22.85772297420817 28.849109534756177 29.08040438803844 19.21276310299721 76-77 23.18716475563571 28.975522292260415 28.706226809781782 19.131086142322097 78-79 23.501967791604773 29.14663155822931 28.30667179881095 19.044728851354968 80-81 23.810147680859217 29.291167803176364 27.942418934700992 18.956265581263423 82-83 24.03770507961464 29.43434718163426 27.614008619414154 18.913939119336945 84-85 24.214548692726332 29.47303443478848 27.42700714946337 18.885409723021812 86-87 24.41474314782592 29.489788023287172 27.248431011714192 18.847037817172716 88-89 24.692975128393826 29.47863979741936 27.049056138552224 18.77932893563459 90-91 24.919070370150322 29.38111136239953 26.939619626668744 18.760198640781407 92-93 25.098674934598602 29.31987804673225 26.879822871769527 18.70162414689962 94-95 25.282351315156067 29.28414509040843 26.779208591513104 18.654295002922403 96-97 25.43685016510745 29.216608401680656 26.68157620136078 18.66496523185112 98-99 25.624230612188864 29.171801733285513 26.548308277433367 18.655659377092253 100 25.725931670879408 29.154941736527856 26.412017417318022 18.707109175274717 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 55425.0 1 41424.0 2 23496.0 3 18396.0 4 17035.5 5 16901.0 6 17806.5 7 18591.5 8 18881.5 9 19457.5 10 20918.5 11 30671.5 12 201047.0 13 244968.0 14 123582.5 15 127314.0 16 142629.0 17 158423.5 18 172813.5 19 187279.0 20 206316.0 21 236533.0 22 278306.5 23 324928.5 24 371257.0 25 421585.0 26 476450.5 27 541241.0 28 633717.5 29 727964.0 30 798233.0 31 873525.0 32 955501.5 33 1013793.0 34 1061871.0 35 1134777.5 36 1217504.0 37 1270098.0 38 1315161.5 39 1351745.0 40 1361249.5 41 1385745.0 42 1440936.0 43 1450798.0 44 1424982.5 45 1417202.5 46 1406851.0 47 1404008.5 48 1384969.0 49 1348926.5 50 1323699.0 51 1294273.0 52 1250122.0 53 1184073.0 54 1097142.0 55 1000351.0 56 899472.5 57 800949.5 58 702851.5 59 593881.0 60 485880.5 61 397313.0 62 313182.0 63 229200.5 64 162453.5 65 114493.5 66 80483.0 67 58915.5 68 43242.5 69 30878.0 70 22591.5 71 17629.0 72 14562.5 73 12262.0 74 10863.0 75 10301.0 76 8219.5 77 4889.5 78 3099.0 79 2496.5 80 2055.5 81 1785.5 82 1518.0 83 1264.5 84 1131.0 85 992.5 86 856.0 87 730.5 88 631.5 89 554.0 90 470.0 91 413.0 92 353.0 93 307.0 94 255.5 95 207.5 96 178.5 97 147.0 98 110.0 99 79.0 100 46.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40462124021438073 2 0.38304137879193473 3 0.1687224601246438 4 0.17509188746806112 5 0.2759156016962468 6 0.10297240871857992 7 0.1363015639057979 8 0.14210187757338105 9 0.1356372107984976 10-11 0.13209592998703162 12-13 0.13416332252373528 14-15 0.14032601061348224 16-17 0.144328389648022 18-19 0.15552250721473537 20-21 0.1364374543141093 22-23 0.09903623270347395 24-25 0.09444034940699285 26-27 0.08896059772824687 28-29 0.09860881671835767 30-31 0.10300609095653745 32-33 0.10419193802393882 34-35 0.10478195791643627 36-37 0.10058445419303909 38-39 0.10011406431811494 40-41 0.10096425046242229 42-43 0.06344688320365749 44-45 0.06635052440689296 46-47 0.08508249453720558 48-49 0.0892765138911589 50-51 0.09856816574151238 52-53 0.1038365323406628 54-55 0.10623145560509142 56-57 0.10693181386331181 58-59 0.10646490835783155 60-61 0.1042395577396719 62-63 0.10858340497971214 64-65 0.11571590923133974 66-67 0.12402496889851834 68-69 0.1291435076115818 70-71 0.1274907550387002 72-73 0.1146438848991052 74-75 0.11201783179489906 76-77 0.11392958916310927 78-79 0.11272283587904462 80-81 0.10690858473368593 82-83 0.10984010089247247 84-85 0.11601788791647623 86-87 0.12935721560413993 88-89 0.13730390084915478 90-91 0.14413674932860848 92-93 0.1435142086546348 94-95 0.13330732909702148 96-97 0.12980205343647563 98-99 0.12489722271597029 100 0.11910039341783099 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4.3049396E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.075020817144406 #Duplication Level Percentage of deduplicated Percentage of total 1 76.64442222948318 29.182379719055774 2 11.427782610310725 8.702261215627633 3 3.9515071619718567 4.51361152353521 4 2.0245016691835103 3.08331772794023 5 1.252160133109723 2.3838011567275506 6 0.7963874841522544 1.8193482022566205 7 0.6188134651830318 1.6492934898141243 8 0.419407287066705 1.2775152948741482 9 0.3510993423805805 1.2031303293023683 >10 2.106723120002646 15.334417183890942 >50 0.22927918583387727 6.088601124686225 >100 0.1618633415013362 13.184463394881424 >500 0.01458929668429549 3.653194398123426 >1k 0.00132955623994936 0.8498026946266809 >5k 9.144334422714485E-5 0.2256239058342746 >10k+ 4.267355212571372E-5 6.849238638823381 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2751634 6.391806286898892 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 72597 0.16863651234502802 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 49308 0.11453819235930744 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.508745999595442E-4 1.3937477775530231E-5 0.0 2.3229129625883716E-6 1.1614564812941859E-5 2 2.6481207773507436E-4 1.8583303700706973E-5 0.0 6.9687388877651156E-6 1.3937477775530231E-5 3 2.717808166228395E-4 2.0906216663295347E-5 0.0 6.9687388877651156E-6 2.0906216663295347E-5 4 2.7642664254801623E-4 3.0197868513648833E-5 0.0 6.9687388877651156E-6 3.0197868513648833E-5 5 2.7642664254801623E-4 3.4843694438825574E-5 0.0 6.9687388877651156E-6 3.0197868513648833E-5 6 2.787495555106046E-4 3.4843694438825574E-5 0.0 9.291651850353486E-6 3.7166607401413945E-5 7 2.8107246847319296E-4 3.948952036400232E-5 0.0 9.291651850353486E-6 3.948952036400232E-5 8 2.833953814357814E-4 4.6458259251767435E-5 0.0 1.1614564812941859E-5 4.4135346289179064E-5 9 2.9268703328613483E-4 5.342699813953255E-5 0.0 1.6260390738118602E-5 4.8781172214355805E-5 10-11 2.9268703328613483E-4 5.5749911102120924E-5 0.0 1.8583303700706973E-5 5.1104085176944176E-5 12-13 2.996557721738999E-4 6.504156295247441E-5 0.0 1.8583303700706973E-5 6.271864998988604E-5 14-15 3.159161629120186E-4 8.014049720929883E-5 0.0 1.8583303700706973E-5 7.433321480282789E-5 16-17 3.3217655365013713E-4 9.059360554094649E-5 0.0 2.0906216663295347E-5 8.594777961576976E-5 18-19 3.600515092011976E-4 1.0104671387259417E-4 0.0 2.0906216663295347E-5 9.640088794741743E-5 20-21 4.0650976845296505E-4 1.1730710461071277E-4 0.0 2.3229129625883717E-5 1.0917690924165347E-4 22-23 5.087179388068534E-4 1.2892166942365463E-4 0.0 2.3229129625883717E-5 1.2427584349847788E-4 24-25 8.606392526389918E-4 1.4866642960565578E-4 0.0 2.3229129625883717E-5 1.3589040831141975E-4 26-27 0.001968668735793645 1.6028099441859765E-4 0.0 2.3229129625883717E-5 1.4634351664306742E-4 28-29 0.005619126456501271 1.742184721941279E-4 0.0 2.9036412032354644E-5 1.5447371201212674E-4 30-31 0.015140746690151006 1.881559499696581E-4 0.0 3.4843694438825574E-5 1.6260390738118602E-4 32-33 0.03441279408426543 1.9744760182001159E-4 0.0 3.8328063882708134E-5 1.6492682034377438E-4 34-35 0.06857703648153392 2.1719236200201276E-4 0.0 4.297388980788488E-5 1.6841118978765696E-4 36-37 0.1234326260930583 2.2764547033366043E-4 0.0 4.7619715733061624E-5 1.8699449348836392E-4 38-39 0.20451506450868673 2.3461420922142554E-4 0.0 4.8781172214355805E-5 2.0325488422648255E-4 40-41 0.3146699201075899 2.450673175530732E-4 0.0 5.2265541658238365E-5 2.137079925581302E-4 42-43 0.457995972812255 2.6945790366025114E-4 0.0 5.5749911102120924E-5 2.2416110088977788E-4 44-45 0.6388045490812462 2.7642664254801623E-4 0.0 5.8072824064709295E-5 2.3112983977754297E-4 46-47 0.8623326561887186 2.892026638422523E-4 0.0 6.271864998988604E-5 2.3693712218401394E-4 48-49 1.1313166856046017 2.9733285921131156E-4 0.0 6.504156295247441E-5 2.5319751292213254E-4 50-51 1.4469901041120297 3.031401416177825E-4 0.0 7.317175832153371E-5 2.6829644717895696E-4 52-53 1.8055166209532882 3.0778596754295925E-4 0.0 7.433321480282789E-5 2.833953814357814E-4 54-55 2.2089915036206316 3.1475470643072434E-4 0.0 7.897904072800465E-5 2.984943156926058E-4 56-57 2.661875906458711 3.2869218420625464E-4 0.0 8.130195369059301E-5 3.0894742402425343E-4 58-59 3.162246457534503 3.5192131383213833E-4 0.0 8.594777961576975E-5 3.2288490179978367E-4 60-61 3.7091507625333464 3.6237442216378597E-4 0.0 8.710923609706395E-5 3.449525749443732E-4 62-63 4.298154380609661 3.6702024808896275E-4 0.0 9.291651850353487E-5 3.588900527199034E-4 64-65 4.92353597713659 3.8328063882708135E-4 0.0 9.756234442871161E-5 3.786348129019046E-4 66-67 5.58870210397377 3.960566601213174E-4 0.0 1.0453108331647673E-4 3.879264647522581E-4 68-69 6.289768850647754 4.0070248604649414E-4 0.0 1.1033836572294765E-4 3.960566601213174E-4 70-71 7.023653711657186 4.1696287678461274E-4 0.0 1.1846856109200695E-4 4.0534831197167086E-4 72-73 7.785306674221399 4.320618110414371E-4 0.0 1.1963001757330115E-4 4.181243332659069E-4 74-75 8.575185352194023 4.459992888169674E-4 0.0 1.2659875646106627E-4 4.2393161567237783E-4 76-77 9.389273429062744 4.634211360363802E-4 0.0 1.31244582386243E-4 4.3786909344790807E-4 78-79 10.228174862197834 4.866502656622639E-4 0.0 1.3589040831141972E-4 4.4483783233567316E-4 80-81 11.091667813411366 5.017491999190883E-4 0.0 1.393747777553023E-4 4.5412948418602667E-4 82-83 11.970304995684494 5.122023082507359E-4 0.0 1.393747777553023E-4 4.634211360363802E-4 84-85 12.867100388586172 5.284626989888546E-4 0.0 1.393747777553023E-4 4.75035700849322E-4 86-87 13.777372161040308 5.470460026895615E-4 0.0 1.416976907178907E-4 4.901346351061464E-4 88 14.467754669542867 5.667907628715628E-4 0.0 1.4402060368047905E-4 4.947804610313231E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 350605 0.0 86.793495 8 AGTGGTA 353615 0.0 86.1896 5 GTGGTAT 354400 0.0 85.95364 6 TGGTATC 353870 0.0 85.79428 7 GTATCAA 356720 0.0 85.211 9 AAGCAGT 360340 0.0 85.03556 1 GCAGTGG 363295 0.0 84.40111 3 CAGTGGT 372270 0.0 81.81864 4 AGCAGTG 377225 0.0 81.30869 2 CAACGCA 349235 0.0 44.850708 12-13 ATCAACG 350030 0.0 44.796524 10-11 AGAGTAC 340890 0.0 44.37964 18-19 ACGCAGA 353310 0.0 44.37365 14-15 TCAACGC 347930 0.0 43.877956 12-13 GAGTACT 334685 0.0 43.671932 20-21 AACGCAG 351425 0.0 43.528732 14-15 CGCAGAG 355240 0.0 43.02278 16-17 TATCAAC 355255 0.0 42.755737 10-11 AGTACTT 350680 0.0 42.52713 20-21 GTACTTT 347855 0.0 41.925087 22-23 >>END_MODULE