FastQCFastQC Report
Thu 26 May 2016
SRR1660716_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660716_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43049396
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT26496466.154897039670429No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA625210.1452308413339876No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT568250.13199952909908425No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT528410.12274504385613215No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3424800.086.419151
GGTATCA3545550.086.278468
GCAGTGG3540050.085.721693
AGTGGTA3546650.085.524515
GTGGTAT3546300.085.460036
TGGTATC3582400.085.350827
GTATCAA3601450.084.859589
AGCAGTG3667750.082.481342
CAGTGGT3717800.081.4778754
CAACGCA3518950.044.62234512-13
ATCAACG3525900.044.6023110-11
ACGCAGA3557700.044.19184514-15
AGAGTAC3503300.043.95243518-19
TCAACGC3502750.043.63939312-13
GAGTACT3431000.043.3374220-21
AACGCAG3536350.043.28658314-15
CGCAGAG3579300.042.74955716-17
TATCAAC3594050.042.52887710-11
AGTACTT3595400.042.31739420-21
GTACTTT3554800.041.7350822-23
Produced by FastQC (version 0.11.5)