##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660716_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 43049396 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.19160538280258 34.0 31.0 34.0 27.0 34.0 2 31.85292478900285 34.0 31.0 34.0 28.0 34.0 3 31.635516233491405 34.0 31.0 34.0 30.0 34.0 4 35.563740127736054 37.0 35.0 37.0 33.0 37.0 5 35.901006648269814 37.0 35.0 37.0 33.0 37.0 6 35.36658263451594 37.0 35.0 37.0 33.0 37.0 7 35.562444290739876 37.0 35.0 37.0 33.0 37.0 8 35.94089389779127 37.0 35.0 37.0 35.0 37.0 9 37.75295699851399 39.0 38.0 39.0 35.0 39.0 10-11 37.755795598154265 39.0 37.5 39.0 35.0 39.0 12-13 37.8508244343312 39.0 38.0 39.0 35.0 39.0 14-15 39.30676505891047 41.0 39.0 41.0 36.0 41.0 16-17 39.27012916743361 41.0 39.0 41.0 36.0 41.0 18-19 39.25552554790781 41.0 39.0 41.0 36.0 41.0 20-21 39.179254071299866 40.5 39.0 41.0 36.0 41.0 22-23 39.02836924820037 40.0 38.5 41.0 35.5 41.0 24-25 39.04672979616253 40.0 39.0 41.0 35.0 41.0 26-27 39.01136720942612 40.0 39.0 41.0 35.0 41.0 28-29 38.93150215162136 40.0 38.5 41.0 35.0 41.0 30-31 38.82829536098485 40.0 38.0 41.0 35.0 41.0 32-33 38.69441402848021 40.0 38.0 41.0 35.0 41.0 34-35 38.59152462905635 40.0 38.0 41.0 34.0 41.0 36-37 38.436125526592754 40.0 38.0 41.0 34.0 41.0 38-39 37.977436617229195 40.0 37.0 41.0 33.5 41.0 40-41 37.83533767814071 40.0 36.5 41.0 33.0 41.0 42-43 37.664243721328866 40.0 36.0 41.0 33.0 41.0 44-45 37.53550496968645 40.0 36.0 41.0 33.0 41.0 46-47 37.380839570896654 40.0 35.0 41.0 33.0 41.0 48-49 37.41679799642253 40.0 35.5 41.0 33.0 41.0 50-51 37.40414461331815 40.0 36.0 41.0 33.0 41.0 52-53 37.13976733843141 40.0 35.0 41.0 31.5 41.0 54-55 36.593463820026656 40.0 35.0 41.0 30.0 41.0 56-57 36.071982972769234 39.0 35.0 41.0 27.5 41.0 58-59 35.91278472292619 39.0 35.0 41.0 27.0 41.0 60-61 35.75729473649294 39.0 35.0 41.0 26.5 41.0 62-63 35.51750461260827 39.0 35.0 41.0 26.0 41.0 64-65 35.25382658098153 38.0 35.0 40.5 26.0 41.0 66-67 34.976150617769406 37.5 35.0 40.0 26.0 41.0 68-69 34.71223873849473 37.0 34.5 40.0 26.0 41.0 70-71 34.41259941254461 36.5 34.0 39.0 25.5 41.0 72-73 34.06204434320054 36.0 34.0 39.0 25.0 41.0 74-75 33.673212871558064 35.5 34.0 39.0 24.5 40.5 76-77 31.964452822985017 34.5 31.5 36.5 21.0 39.0 78-79 32.87543568555526 35.0 33.0 37.0 23.0 39.0 80-81 32.76035762499432 35.0 34.0 37.0 23.0 39.0 82-83 32.485170883698345 35.0 33.5 36.0 21.5 38.0 84-85 32.22024324104338 35.0 33.0 36.0 20.0 37.0 86-87 31.99341422815781 35.0 33.0 35.5 20.0 37.0 88-89 31.806157779774658 35.0 33.0 35.0 20.0 36.5 90-91 31.63150912732899 35.0 33.0 35.0 19.5 36.0 92-93 31.476353268696265 35.0 33.0 35.0 19.0 36.0 94-95 31.35554267706799 35.0 33.0 35.0 19.0 36.0 96-97 31.240837595026882 35.0 33.0 35.0 18.0 35.0 98-99 31.139282987849587 35.0 33.0 35.0 18.0 35.0 100 31.078921873839995 35.0 33.0 35.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.6204685512778205 1101 2 1.212270990459519 1101 3 1.5663125613007303 1101 4 0.9718889347175903 1101 5 0.5776969115496797 1101 6 1.135511528941457 1101 7 0.821141490558766 1101 8 0.48349221582398627 1101 9 0.5114232071996128 1101 10-11 0.4327123089711904 1101 12-13 0.41687781676088775 1101 14-15 0.5220468067105983 1101 16-17 0.5529607218611048 1101 18-19 0.5202512556511536 1101 20-21 0.532723814705065 1101 22-23 0.5114433931632973 1101 24-25 0.5061216557902881 1101 26-27 0.49803278541707385 1101 28-29 0.4762886494198355 1101 30-31 0.5154580346059703 1101 32-33 0.5505353573464475 1101 34-35 0.54088006026047 1101 36-37 0.5326868518810244 1101 38-39 0.5550194217363824 1101 40-41 0.5518144552700193 1101 42-43 0.5458966437516324 1101 44-45 0.556630632469755 1101 46-47 0.5659957896981851 1101 48-49 0.5400975212002024 1101 50-51 0.4938671569063402 1101 52-53 0.5221525786640271 1101 54-55 0.5348990299283756 1101 56-57 0.5613636898464449 1101 58-59 0.5426080614799034 1101 60-61 0.5671242968243178 1101 62-63 0.5740466073139103 1101 64-65 0.5424056163189448 1101 66-67 0.5697683224103827 1101 68-69 0.5300591365471874 1101 70-71 0.5173001070469283 1101 72-73 0.5097907281029563 1101 74-75 0.5152672142264194 1101 76-77 0.4304401018605901 1101 78-79 0.53854432970423 1101 80-81 0.515933144146814 1101 82-83 0.5137232785331989 1101 84-85 0.5291351043773815 1101 86-87 0.486004115546514 1101 88-89 0.4853591788634972 1101 90-91 0.5251347290495794 1101 92-93 0.513629900094589 1101 94-95 0.4608726710887012 1101 96-97 0.47693516237123745 1101 98-99 0.4869558210949485 1101 100 0.5057792024137484 1102 1 1.5072370236998474 1102 2 1.143926412104225 1102 3 1.499756706642824 1102 4 0.9122660802379272 1102 5 0.5080032680666093 1102 6 1.0636645589567877 1102 7 0.7862841807553025 1102 8 0.42987762812688857 1102 9 0.46203185304622707 1102 10-11 0.37672270381653306 1102 12-13 0.3462833953524722 1102 14-15 0.4631305016525644 1102 16-17 0.4841204726244257 1102 18-19 0.42093894972943957 1102 20-21 0.4092651988344187 1102 22-23 0.44289599391836276 1102 24-25 0.43435546185646956 1102 26-27 0.4450226212874213 1102 28-29 0.4233097252682825 1102 30-31 0.4172956846806599 1102 32-33 0.45949767677233666 1102 34-35 0.414325431435671 1102 36-37 0.412662486813133 1102 38-39 0.40953720984814623 1102 40-41 0.44220391621966826 1102 42-43 0.4641814799507884 1102 44-45 0.4274009992912937 1102 46-47 0.4349786368176254 1102 48-49 0.42423486970239566 1102 50-51 0.3821324973950979 1102 52-53 0.3785535196511276 1102 54-55 0.40969163034407785 1102 56-57 0.4315175875425368 1102 58-59 0.4308858567056859 1102 60-61 0.44346224909807574 1102 62-63 0.39257670594633254 1102 64-65 0.38996545890518064 1102 66-67 0.37601708175714776 1102 68-69 0.36743170227873634 1102 70-71 0.34749071014223176 1102 72-73 0.31971145968439174 1102 74-75 0.37201567077622144 1102 76-77 0.17988774978115174 1102 78-79 0.37034695916594984 1102 80-81 0.40730371514142405 1102 82-83 0.4225125077561387 1102 84-85 0.44234907828710845 1102 86-87 0.43778762723726317 1102 88-89 0.41146999559656194 1102 90-91 0.4169093432226738 1102 92-93 0.4276281752887634 1102 94-95 0.3922786926967383 1102 96-97 0.3879209707480804 1102 98-99 0.41995115683068107 1102 100 0.41075161862029574 1103 1 1.4827018775370782 1103 2 1.0809121931009322 1103 3 1.4349629262900727 1103 4 0.8854989467732892 1103 5 0.48610282625919865 1103 6 1.0147827992740233 1103 7 0.7533346627119855 1103 8 0.4197903465794397 1103 9 0.41803097624742236 1103 10-11 0.3275591870685659 1103 12-13 0.2970403027458701 1103 14-15 0.35111462815401495 1103 16-17 0.3913146875694551 1103 18-19 0.3429006372694161 1103 20-21 0.3617089802591522 1103 22-23 0.3409228313430148 1103 24-25 0.3559683800578952 1103 26-27 0.36699102506307213 1103 28-29 0.35465282986711344 1103 30-31 0.3558509105037402 1103 32-33 0.3865985151547733 1103 34-35 0.3907606840526583 1103 36-37 0.3432743687214952 1103 38-39 0.3572713036105526 1103 40-41 0.37247195930970634 1103 42-43 0.37754628228770315 1103 44-45 0.3623067907242543 1103 46-47 0.35388450521561055 1103 48-49 0.3478102961502927 1103 50-51 0.31512310586303727 1103 52-53 0.313733441337682 1103 54-55 0.36961448251650353 1103 56-57 0.38506204402830235 1103 58-59 0.35452240082880593 1103 60-61 0.361739919818163 1103 62-63 0.3150651663472601 1103 64-65 0.3006937506737515 1103 66-67 0.3305763902205001 1103 68-69 0.3064993384602275 1103 70-71 0.30372071037219683 1103 72-73 0.28152608179912164 1103 74-75 0.29398184692394125 1103 76-77 0.13648653117500942 1103 78-79 0.30585474898210663 1103 80-81 0.32700020176667977 1103 82-83 0.3219046907198404 1103 84-85 0.2993396396093715 1103 86-87 0.25980744488794727 1103 88-89 0.28293209789778473 1103 90-91 0.2854531025012008 1103 92-93 0.28530720427044187 1103 94-95 0.27863119603719255 1103 96-97 0.3255179767877898 1103 98-99 0.3167269980831833 1103 100 0.30733385635396004 1104 1 1.4350060955330939 1104 2 1.0616295323290181 1104 3 1.407418832696134 1104 4 0.8489661570398113 1104 5 0.4813279862090454 1104 6 1.0013753076563816 1104 7 0.7558055824319254 1104 8 0.41911037716769073 1104 9 0.41263737611139817 1104 10-11 0.3244787913837257 1104 12-13 0.28691352664232994 1104 14-15 0.3459346841103894 1104 16-17 0.3718104451246731 1104 18-19 0.34694166196275233 1104 20-21 0.3566267598228947 1104 22-23 0.3394520041786109 1104 24-25 0.3407844451010007 1104 26-27 0.3390540465137022 1104 28-29 0.3042944291436527 1104 30-31 0.32488951231216845 1104 32-33 0.33923886724547714 1104 34-35 0.3724840971972654 1104 36-37 0.3673599449601852 1104 38-39 0.3982165787504428 1104 40-41 0.3813980830174444 1104 42-43 0.38812426862738647 1104 44-45 0.36991240915416057 1104 46-47 0.3682852497276343 1104 48-49 0.33128906427673854 1104 50-51 0.33659883514766165 1104 52-53 0.33968793167365874 1104 54-55 0.3710830954490305 1104 56-57 0.4313908525386978 1104 58-59 0.44711247171166946 1104 60-61 0.44257718771435606 1104 62-63 0.3823493818269199 1104 64-65 0.3319090909337419 1104 66-67 0.3446466878549046 1104 68-69 0.2916576715746757 1104 70-71 0.31696819031152756 1104 72-73 0.319755425124967 1104 74-75 0.3092860319549757 1104 76-77 0.2017512778340631 1104 78-79 0.3286432510163948 1104 80-81 0.36693200765125766 1104 82-83 0.3697841313550114 1104 84-85 0.3515402492936488 1104 86-87 0.3862086421233144 1104 88-89 0.3699916723731036 1104 90-91 0.3990242700084927 1104 92-93 0.37785149957796804 1104 94-95 0.3633193770912193 1104 96-97 0.3311517164033866 1104 98-99 0.35256745001220224 1104 100 0.39192203630497957 1105 1 1.3776619972060722 1105 2 1.0046529321065272 1105 3 1.3493986035334835 1105 4 0.8423753975029769 1105 5 0.4357272305146367 1105 6 0.9914104787527904 1105 7 0.7044197699343613 1105 8 0.36046346080169656 1105 9 0.3447336535671539 1105 10-11 0.26346896845885937 1105 12-13 0.2270623887074521 1105 14-15 0.3118317939808293 1105 16-17 0.2993518023379522 1105 18-19 0.2772832683880111 1105 20-21 0.28888051014175176 1105 22-23 0.2931870628735851 1105 24-25 0.28213740323423053 1105 26-27 0.3084859702049414 1105 28-29 0.2819801727338245 1105 30-31 0.2816176806276687 1105 32-33 0.3436858506991669 1105 34-35 0.3419544547356921 1105 36-37 0.3187920595301392 1105 38-39 0.3416561299159824 1105 40-41 0.33183919938701933 1105 42-43 0.3319484123274563 1105 44-45 0.3384542421659873 1105 46-47 0.36731532341352846 1105 48-49 0.32018450528561715 1105 50-51 0.34347265770398394 1105 52-53 0.35673909206594345 1105 54-55 0.4109148204831925 1105 56-57 0.39801285488748306 1105 58-59 0.35499241586276753 1105 60-61 0.32798907823475076 1105 62-63 0.27792883030578963 1105 64-65 0.2775115065442648 1105 66-67 0.29563065201231353 1105 68-69 0.2731559230207381 1105 70-71 0.28569069607612363 1105 72-73 0.263067534232448 1105 74-75 0.293923578773466 1105 76-77 0.17943977938029576 1105 78-79 0.2841517464748833 1105 80-81 0.2949277039958105 1105 82-83 0.29617315697853286 1105 84-85 0.29564967094883343 1105 86-87 0.29638859919107574 1105 88-89 0.29498360132641466 1105 90-91 0.3088050103343143 1105 92-93 0.3180803962424186 1105 94-95 0.2799974667772389 1105 96-97 0.2956590998662243 1105 98-99 0.28034970810643856 1105 100 0.28762156943548334 1106 1 1.3693171510702449 1106 2 1.0029657947194117 1106 3 1.3332158743955667 1106 4 0.7867493341567737 1106 5 0.4038455538777015 1106 6 0.9258325727351462 1106 7 0.6663386178600561 1106 8 0.31661798350874903 1106 9 0.30189518331089005 1106 10-11 0.22356186710829462 1106 12-13 0.18434783275991862 1106 14-15 0.22908534711202577 1106 16-17 0.23869559025614961 1106 18-19 0.20254508540101313 1106 20-21 0.23000345448410542 1106 22-23 0.2579009175135738 1106 24-25 0.24516007563735087 1106 26-27 0.2126701761635985 1106 28-29 0.22648906287335535 1106 30-31 0.23131500467626864 1106 32-33 0.2853160015913332 1106 34-35 0.2653404843301317 1106 36-37 0.2523823904975657 1106 38-39 0.2812510791799454 1106 40-41 0.2530522512771256 1106 42-43 0.29586331332915705 1106 44-45 0.27342401191190646 1106 46-47 0.3092125975297293 1106 48-49 0.26633236750794964 1106 50-51 0.29270215681288647 1106 52-53 0.2785271056234535 1106 54-55 0.33655341965259566 1106 56-57 0.37077659237546357 1106 58-59 0.33728970624951415 1106 60-61 0.30609006747448575 1106 62-63 0.27245862159786327 1106 64-65 0.24181620469266107 1106 66-67 0.2322213577472354 1106 68-69 0.24691602456687178 1106 70-71 0.25685879212846885 1106 72-73 0.21126023373336977 1106 74-75 0.2509431037345067 1106 76-77 0.12794346360657727 1106 78-79 0.26803817080212156 1106 80-81 0.3126572541133257 1106 82-83 0.33010392808547095 1106 84-85 0.34745830764667573 1106 86-87 0.3323863869224013 1106 88-89 0.3380190340134206 1106 90-91 0.3374980998032946 1106 92-93 0.32515594936422687 1106 94-95 0.32750304108256145 1106 96-97 0.3408806158815061 1106 98-99 0.3328111115648902 1106 100 0.3372994818306232 1107 1 1.3007156269062712 1107 2 0.9341263624729272 1107 3 1.2824736653630104 1107 4 0.7781319347548106 1107 5 0.3693100315307305 1107 6 0.9355946434875833 1107 7 0.6393323490348095 1107 8 0.2947081492532959 1107 9 0.25483808901139327 1107 10-11 0.17737902254869198 1107 12-13 0.13080183889891117 1107 14-15 0.16992256806465633 1107 16-17 0.17999213285309423 1107 18-19 0.14295099177183346 1107 20-21 0.13409557108451509 1107 22-23 0.18086489739437894 1107 24-25 0.17772916241359127 1107 26-27 0.15418957287182877 1107 28-29 0.16213010649290283 1107 30-31 0.15743235736746186 1107 32-33 0.19938440056674978 1107 34-35 0.21859667304014607 1107 36-37 0.20039752392180787 1107 38-39 0.19763714016807654 1107 40-41 0.22592130258335175 1107 42-43 0.23693168818891053 1107 44-45 0.19185542471941375 1107 46-47 0.24630055686433394 1107 48-49 0.21741191044924335 1107 50-51 0.19551644404730695 1107 52-53 0.18534313239506872 1107 54-55 0.23145036873892622 1107 56-57 0.2369231511124923 1107 58-59 0.18769641790554914 1107 60-61 0.2088477874797121 1107 62-63 0.18484252330386397 1107 64-65 0.17210715983699032 1107 66-67 0.17478473740422373 1107 68-69 0.18619889962032232 1107 70-71 0.2091135618570803 1107 72-73 0.16507726524394428 1107 74-75 0.18360508022418998 1107 76-77 0.04436327041626953 1107 78-79 0.1819567739137682 1107 80-81 0.243290178788385 1107 82-83 0.270806966985063 1107 84-85 0.26864688961829586 1107 86-87 0.2346172435294065 1107 88-89 0.23647122853762426 1107 90-91 0.24114170925298595 1107 92-93 0.2514706708067216 1107 94-95 0.23717191461393483 1107 96-97 0.255900892621888 1107 98-99 0.2816631080647589 1107 100 0.2670201332100035 1108 1 1.2723018557302233 1108 2 0.9001246309150694 1108 3 1.2481811671657148 1108 4 0.7632578203411313 1108 5 0.3616257223885242 1108 6 0.8898013430211122 1108 7 0.623121178361707 1108 8 0.27559729971488167 1108 9 0.21811365081195078 1108 10-11 0.13280465417366116 1108 12-13 0.1250462339898064 1108 14-15 0.1469046956140616 1108 16-17 0.15050208968916223 1108 18-19 0.09679399589759186 1108 20-21 0.1341195480494406 1108 22-23 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-0.8942792869138501 2316 92-93 -0.9585465268018147 2316 94-95 -0.9891726679852049 2316 96-97 -1.0756640065497045 2316 98-99 -1.185041504038022 2316 100 -1.1373975201430575 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 90.0 7 1330.0 8 1550.0 9 613.0 10 591.0 11 1167.0 12 1662.0 13 3450.0 14 11265.0 15 31091.0 16 57505.0 17 79963.0 18 94227.0 19 103884.0 20 120244.0 21 161565.0 22 238147.0 23 381415.0 24 616403.0 25 813234.0 26 814401.0 27 667927.0 28 568121.0 29 579640.0 30 660778.0 31 790501.0 32 964337.0 33 1221348.0 34 1652476.0 35 2515807.0 36 4493201.0 37 9078808.0 38 1.251248E7 39 3802791.0 40 7384.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 15.071301004742057 34.01146139150867 29.115810208604408 21.801427395144863 2 19.91892285242605 35.39347250094113 27.074730893724496 17.612873752908325 3 25.823428195227837 26.197002447718766 27.07650664555906 20.90306271149434 4 18.697124944269913 28.361477496497074 25.110787103136335 27.83061045609668 5 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26.096745578115748 23.473464606133515 26-27 19.567729481466397 27.2202854845118 33.60867426194927 19.60331077207254 28-29 19.574691236731134 27.305800282569376 33.576092526423615 19.543415954275876 30-31 19.532318718261337 27.21597878949214 33.58844074618918 19.66326174605734 32-33 19.719135731121927 27.315569564333035 33.34835131173339 19.616943392811653 34-35 19.71075633412742 27.401376622872775 33.2631156625031 19.624751380496708 36-37 19.65287620976487 27.30782563180404 33.35387047633844 19.685427682092644 38-39 19.698408589035626 27.275126652217306 33.366206229688174 19.6602585290589 40-41 19.747310150263605 27.25301351622668 33.36745973378039 19.632216599729325 42-43 19.74413454936002 27.27966639325044 33.30497020931019 19.67122884807935 44-45 19.792620099396675 27.353067862767013 33.22159066397974 19.63272137385658 46-47 19.824139084462246 27.428304238314986 33.12759606131222 19.619960615910554 48-49 19.82529125908335 27.464214908678148 33.00660206311045 19.70389176912806 50-51 19.846069875020877 27.534182407064478 32.897135429148236 19.722612288766403 52-53 19.970540687580986 27.657051086381006 32.60948105604227 19.762927169995738 54-55 20.33128050562991 27.872019577280145 31.87232012200991 19.924379795080032 56-57 20.833568192477404 28.45648611098397 30.601117584110515 20.108828112428114 58-59 20.98139990909853 29.058246057956737 29.79265422924739 20.167699803697342 60-61 21.056537700832482 29.411789579133853 29.347081473699888 20.184591246333778 62-63 21.07544409030683 29.616653103331704 29.052647231888884 20.25525557447258 64-65 21.116104366521217 29.74965352115373 28.87371437500738 20.260527737317673 66-67 21.082708458382402 29.6391783274711 28.948353773741943 20.32975944040456 68-69 21.022560169968425 29.49987527237215 29.062067179821472 20.415497377837955 70-71 20.942941456770576 29.389746233157325 29.151547025766288 20.515765284305807 72-73 20.900535528607627 29.303822108100423 29.11011999928219 20.685522364009756 74-75 20.947736298553725 29.32172526711461 28.92310135322665 20.80743708110502 76-77 20.96799447793338 29.412938749698643 28.700703024533137 20.918363747834846 78-79 21.058551063886085 29.581809126206622 28.312571322330164 21.04706848757713 80-81 21.145976158372207 29.713123586192985 27.9633812748053 21.177518980629507 82-83 21.17400902362523 29.810442655349227 27.619645025871726 21.39590329515381 84-85 21.190558888593085 29.800678558384735 27.399344868229363 21.609417684792813 86-87 21.227315525327796 29.77815299061209 27.204903395672275 21.78962808838784 88-89 21.298486064222562 29.754085385866276 27.020748163017494 21.926680386893665 90-91 21.40169434847531 29.644023095256046 26.8891419760804 22.06514058018825 92-93 21.486831790187182 29.55133414140272 26.8427633127975 22.119070755612597 94-95 21.579109057766605 29.484186438512328 26.782983836275648 22.15372066744542 96-97 21.629831878174382 29.399529873511703 26.754186192312513 22.2164520560014 98-99 21.62812214269624 29.32816120706362 26.767721886431755 22.275994763808384 100 21.56668736678923 29.276643617763177 26.764972735030103 22.391696280417495 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 37663.0 1 29927.0 2 18270.5 3 13393.5 4 12477.5 5 12664.5 6 13477.0 7 14151.5 8 14470.0 9 15395.5 10 17945.0 11 35402.5 12 212100.0 13 246736.5 14 117065.0 15 122457.0 16 140066.5 17 157175.0 18 172455.0 19 188442.0 20 208326.0 21 240011.0 22 285142.5 23 334412.5 24 382098.0 25 434496.5 26 490630.5 27 556032.5 28 649037.0 29 743905.5 30 816026.5 31 890791.5 32 970569.0 33 1027746.5 34 1073625.5 35 1141120.5 36 1219919.5 37 1271569.5 38 1317363.0 39 1354772.0 40 1366183.5 41 1394127.0 42 1450758.0 43 1459916.5 44 1433086.5 45 1423627.0 46 1411338.5 47 1400352.5 48 1372395.0 49 1335285.5 50 1313497.5 51 1290243.0 52 1251911.5 53 1188996.5 54 1101366.0 55 1003305.5 56 899135.0 57 796010.0 58 694151.5 59 583700.5 60 475906.0 61 385493.0 62 301051.0 63 217687.5 64 151303.5 65 104957.5 66 72150.0 67 51920.5 68 37296.5 69 25761.0 70 17976.0 71 13277.0 72 10636.5 73 8518.5 74 7453.0 75 7338.0 76 5510.5 77 2469.0 78 900.0 79 518.0 80 361.0 81 257.5 82 180.5 83 138.5 84 108.5 85 92.5 86 77.0 87 54.0 88 34.0 89 19.0 90 15.0 91 12.5 92 8.5 93 10.0 94 8.0 95 4.0 96 3.0 97 2.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32954237035056194 2 0.006367104430454728 3 2.3979755720614526 4 8.989673165216999E-4 5 4.8781172214355805E-5 6 0.008597100874539564 7 0.007649352385803508 8 3.2520781476237204E-4 9 0.0 10-11 6.736447591506278E-5 12-13 3.4843694438825574E-6 14-15 0.0 16-17 2.787495555106046E-5 18-19 0.0 20-21 2.9152557680484065E-4 22-23 4.0650976845296505E-5 24-25 4.971033739939115E-4 26-27 0.0034646246837005566 28-29 0.0060918392443880046 30-31 0.005645839955571038 32-33 0.01519417368829054 34-35 0.009758557355833749 36-37 0.005082533562143357 38-39 0.00529972592414537 40-41 0.008231242082931895 42-43 0.019002589490454174 44-45 0.03404577383617647 46-47 0.03310383262984688 48-49 0.039698582530635275 50-51 0.039973847716701996 52-53 0.0619462814298254 54-55 0.03299581717708652 56-57 0.021867902629806934 58-59 0.016694775462122627 60-61 0.017764476881394573 62-63 0.02455551292752168 64-65 0.04495301165201017 66-67 0.04751170028030126 68-69 0.05026667505393107 70-71 0.06077437184019956 72-73 0.06846089083340448 74-75 0.05954787379595291 76-77 0.05957923312094785 78-79 0.05421098126440613 80-81 0.05150943348891585 82-83 0.04778231964044281 84-85 0.04973588944197963 86-87 0.057900928505477754 88-89 0.06447477218960285 90-91 0.06043058072173649 92-93 0.06486850593676158 94-95 0.07330300290391994 96-97 0.07244236365128097 98-99 0.04741762230531643 100 0.006857239065560874 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4.3049396E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.64850429665769 #Duplication Level Percentage of deduplicated Percentage of total 1 75.55013301476258 28.443495074193486 2 11.918575904968828 8.974331123365195 3 4.20647643241426 4.7510263811861275 4 2.180614509979952 3.2838749899333735 5 1.2720288317360453 2.3944991468543484 6 0.8579827891829785 1.938106123500823 7 0.6212950968464943 1.6373581786182316 8 0.45993336583386074 1.3852642639817871 9 0.35628660420792974 1.2072291974427438 >10 2.161176593221395 15.662278567597868 >50 0.23648631393265213 6.186826757371851 >100 0.16449584259897193 13.173596681678085 >500 0.013294709300718218 3.264120268854164 >1k 0.001096059773279623 0.753808176789796 >5k 6.196562774618299E-5 0.14852957931900018 >10k+ 6.196561232837933E-5 6.795655489312937 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2649646 6.154897039670429 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 62521 0.1452308413339876 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 56825 0.13199952909908425 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 52841 0.12274504385613215 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.3461420922142554E-4 9.291651850353486E-6 0.0 0.0 2.3229129625883716E-6 2 2.4855168699695576E-4 1.6260390738118602E-5 0.0 0.0 9.291651850353486E-6 3 2.555204258847209E-4 2.0906216663295347E-5 0.0 2.3229129625883716E-6 1.6260390738118602E-5 4 2.62489164772486E-4 3.948952036400232E-5 0.0 4.645825925176743E-6 1.6260390738118602E-5 5 2.62489164772486E-4 5.342699813953255E-5 0.0 4.645825925176743E-6 1.8583303700706973E-5 6 2.6481207773507436E-4 6.0395737027297666E-5 0.0 6.9687388877651156E-6 3.0197868513648833E-5 7 2.6945790366025114E-4 6.736447591506278E-5 0.0 9.291651850353486E-6 3.7166607401413945E-5 8 2.6945790366025114E-4 7.665612776541627E-5 0.0 9.291651850353486E-6 3.948952036400232E-5 9 2.787495555106046E-4 8.594777961576975E-5 0.0 1.3937477775530231E-5 4.6458259251767435E-5 10-11 2.9036412032354647E-4 9.756234442871161E-5 0.0 1.5098934256824416E-5 5.342699813953255E-5 12-13 3.1127033698684185E-4 1.1033836572294767E-4 0.0 1.6260390738118602E-5 6.155719350859186E-5 14-15 3.3217655365013713E-4 1.3472895183012557E-4 0.0 1.6260390738118602E-5 7.084884535894534E-5 16-17 3.5075985735084415E-4 1.4518206016177323E-4 0.0 1.974476018200116E-5 7.897904072800465E-5 18-19 3.821191823457871E-4 1.5911953793730347E-4 0.0 2.5552042588472088E-5 8.130195369059301E-5 20-21 4.285774415975546E-4 1.7886429811930462E-4 0.0 2.6713499069766273E-5 9.640088794741743E-5 22-23 5.37754350839208E-4 2.0209342774518834E-4 0.0 2.7874955551060462E-5 1.0801545276035928E-4 24-25 8.780610998584045E-4 2.0557779718907091E-4 0.0 3.2520781476237204E-5 1.2776021294236045E-4 26-27 0.002023257190414472 2.1835381848330694E-4 0.0 3.600515092011976E-5 1.3589040831141972E-4 28-29 0.005752693951850103 2.3809857866530812E-4 0.0 3.8328063882708134E-5 1.4634351664306742E-4 30-31 0.015364907791040786 2.543589694034267E-4 0.0 4.4135346289179064E-5 1.6144245089989184E-4 32-33 0.034848340264750755 2.6365062125378023E-4 0.0 4.7619715733061624E-5 1.6957264626895114E-4 34-35 0.06957124322952174 2.775880990293104E-4 0.0 5.1104085176944176E-5 1.7654138515671626E-4 36-37 0.125113253621491 2.880412073609581E-4 0.0 5.9234280546003484E-5 1.8467158052577553E-4 38-39 0.20734901832304453 2.996557721738999E-4 0.0 6.504156295247441E-5 1.962861453387174E-4 40-41 0.3191635952337171 3.0894742402425343E-4 0.0 6.504156295247441E-5 2.12546536076836E-4 42-43 0.4642782909195753 3.205619888371953E-4 0.0 6.504156295247441E-5 2.2183818792718952E-4 44-45 0.646731489566079 3.2288490179978367E-4 0.0 6.852593239635697E-5 2.2764547033366043E-4 46-47 0.8721980675408314 3.3566092309401973E-4 0.0 7.201030184023953E-5 2.4042149162789648E-4 48-49 1.1435433379831856 3.4611403142566737E-4 0.0 7.433321480282789E-5 2.5784333884730927E-4 50-51 1.4621447882799563 3.635358786450802E-4 0.0 8.014049720929883E-5 2.659735342163686E-4 52-53 1.8247085278501933 3.774733564206104E-4 0.0 8.827069257835813E-5 2.775880990293104E-4 54-55 2.23289776237511 3.9373374715872904E-4 0.0 8.827069257835813E-5 2.880412073609581E-4 56-57 2.6917357911363027 4.1580142030331856E-4 0.0 9.523943146612325E-5 2.9268703328613483E-4 58-59 3.1985082903369886 4.2973889807884874E-4 0.0 9.988525739129999E-5 2.961714027300174E-4 60-61 3.7524870267633954 4.4019200641049643E-4 0.0 9.988525739129999E-5 3.0778596754295925E-4 62-63 4.349407132216211 4.4948365826084994E-4 0.0 1.0104671387259417E-4 3.1359324994943016E-4 64-65 4.982076868163261 4.634211360363802E-4 0.0 1.0801545276035929E-4 3.286921842062546E-4 66-67 5.655325570653767 4.831658962183813E-4 0.0 1.149841916481244E-4 3.4727548790696155E-4 68-69 6.365469099729065 4.901346351061464E-4 0.0 1.1730710461071277E-4 3.6585879160766857E-4 70-71 7.109177559657283 4.947804610313231E-4 0.0 1.1846856109200695E-4 3.716660740141395E-4 72-73 7.880518230731971 5.110408517694418E-4 0.0 1.231143870171837E-4 3.75150443458022E-4 74-75 8.68155734403335 5.203325036197953E-4 0.0 1.231143870171837E-4 3.80957725864493E-4 76-77 9.507172876478919 5.331085249140313E-4 0.0 1.2776021294236045E-4 3.9024937771484644E-4 78-79 10.357844509595441 5.4936891565215E-4 0.0 1.3008312590494881E-4 3.9954102956519995E-4 80-81 11.231588475712876 5.749209582406221E-4 0.0 1.3008312590494881E-4 4.030253990090825E-4 82-83 12.121452296334192 5.93504261941329E-4 0.0 1.3240603886753718E-4 4.099941378968476E-4 84-85 13.029586988862746 6.039573702729767E-4 0.0 1.3472895183012557E-4 4.204472462284953E-4 86-87 13.952537220266692 6.086031961981534E-4 0.0 1.3589040831141975E-4 4.2973889807884874E-4 88 14.65252381241307 6.132490221233301E-4 0.0 1.3705186479271393E-4 4.367076369666139E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 342480 0.0 86.41915 1 GGTATCA 354555 0.0 86.27846 8 GCAGTGG 354005 0.0 85.72169 3 AGTGGTA 354665 0.0 85.52451 5 GTGGTAT 354630 0.0 85.46003 6 TGGTATC 358240 0.0 85.35082 7 GTATCAA 360145 0.0 84.85958 9 AGCAGTG 366775 0.0 82.48134 2 CAGTGGT 371780 0.0 81.477875 4 CAACGCA 351895 0.0 44.622345 12-13 ATCAACG 352590 0.0 44.60231 10-11 ACGCAGA 355770 0.0 44.191845 14-15 AGAGTAC 350330 0.0 43.952435 18-19 TCAACGC 350275 0.0 43.639393 12-13 GAGTACT 343100 0.0 43.33742 20-21 AACGCAG 353635 0.0 43.286583 14-15 CGCAGAG 357930 0.0 42.749557 16-17 TATCAAC 359405 0.0 42.528877 10-11 AGTACTT 359540 0.0 42.317394 20-21 GTACTTT 355480 0.0 41.73508 22-23 >>END_MODULE