FastQCFastQC Report
Thu 26 May 2016
SRR1660715_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660715_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27563958
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11636814.221748560203147No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT462080.16763920479054567No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA377790.13705941650324674No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1520750.082.662368
AGTGGTA1543650.081.635495
GTGGTAT1545550.081.521316
TGGTATC1541550.081.3857047
GTATCAA1547150.081.147619
AAGCAGT1615150.078.266921
GCAGTGG1630900.077.6334153
CAGTGGT1685150.074.6452944
AGCAGTG1741900.072.762942
CAACGCA1497850.044.31385412-13
ATCAACG1507200.044.13054710-11
ACGCAGA1520600.043.710614-15
AGAGTAC1518000.043.0679618-19
TCAACGC1493950.042.2112112-13
GAGTACT1470850.041.974220-21
AACGCAG1509800.041.87777714-15
CGCAGAG1538200.041.0664916-17
AGTACTT1580050.040.86273620-21
TATCAAC1543800.040.64862410-11
GTACTTT1581500.039.021222-23
Produced by FastQC (version 0.11.5)