##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660715_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 27563958 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.933351117426604 34.0 31.0 34.0 30.0 34.0 2 32.06927357819947 34.0 31.0 34.0 30.0 34.0 3 32.12183268455133 34.0 31.0 34.0 30.0 34.0 4 35.53503571584313 37.0 35.0 37.0 33.0 37.0 5 35.501171928937055 37.0 35.0 37.0 33.0 37.0 6 35.55529474395513 37.0 35.0 37.0 33.0 37.0 7 35.50915634104507 37.0 35.0 37.0 33.0 37.0 8 35.512572577566694 37.0 35.0 37.0 33.0 37.0 9 37.18347691576079 39.0 37.0 39.0 34.0 39.0 10-11 37.155672581564666 39.0 37.0 39.0 34.0 39.0 12-13 37.15284849512541 39.0 37.0 39.0 33.5 39.0 14-15 38.51975024776921 40.0 38.0 41.0 34.0 41.0 16-17 38.500578690476885 40.0 38.0 41.0 34.0 41.0 18-19 38.47045034316189 40.0 38.0 41.0 34.0 41.0 20-21 38.36373754814167 40.0 38.0 41.0 33.5 41.0 22-23 38.211713390362874 40.0 38.0 41.0 33.5 41.0 24-25 38.17863307221698 40.0 38.0 41.0 33.0 41.0 26-27 38.135708231742335 40.0 38.0 41.0 33.0 41.0 28-29 38.04364023120337 40.0 38.0 41.0 33.0 41.0 30-31 37.953494723072794 40.0 38.0 41.0 33.0 41.0 32-33 37.832311854487656 40.0 38.0 41.0 32.0 41.0 34-35 37.67968225027769 40.0 37.5 41.0 32.0 41.0 36-37 37.503319280199165 40.0 37.0 41.0 31.0 41.0 38-39 37.14834518685596 40.0 36.0 41.0 31.0 41.0 40-41 37.096059734962594 40.0 35.5 41.0 31.0 41.0 42-43 37.115557098149694 40.0 36.0 41.0 31.0 41.0 44-45 37.12375573928824 40.0 36.0 41.0 31.0 41.0 46-47 37.22398918544282 40.0 36.0 41.0 31.0 41.0 48-49 37.12121700736883 40.0 36.0 41.0 31.0 41.0 50-51 35.76303134694952 38.5 34.0 40.0 29.0 40.5 52-53 35.95743639211756 38.5 35.0 39.5 29.5 40.5 54-55 36.44782558078198 39.0 35.0 41.0 28.5 41.0 56-57 36.16026009399666 39.0 35.0 41.0 27.5 41.0 58-59 35.977462561799 39.0 35.0 41.0 27.0 41.0 60-61 35.742539750641036 39.0 35.0 41.0 27.0 41.0 62-63 35.505492244618864 38.5 35.0 41.0 26.0 41.0 64-65 35.243363979149876 38.0 35.0 40.5 26.0 41.0 66-67 34.96249928983349 37.0 34.0 40.0 26.0 41.0 68-69 34.672928974859126 37.0 34.0 40.0 26.0 41.0 70-71 34.36591655305816 36.0 34.0 39.0 26.0 41.0 72-73 34.022083493959755 36.0 34.0 39.0 26.0 41.0 74-75 33.64782947717451 35.0 33.5 38.5 25.0 40.0 76-77 33.25876564969371 35.0 33.0 37.0 24.5 39.5 78-79 32.89193384346326 35.0 33.0 37.0 24.0 39.0 80-81 32.57788661918582 35.0 33.0 36.0 23.5 39.0 82-83 32.27563913353808 35.0 33.0 36.0 23.0 37.5 84-85 32.00186029161705 35.0 33.0 35.5 22.5 37.0 86-87 31.797162149209484 35.0 32.0 35.0 21.5 37.0 88-89 31.600791874664736 35.0 32.0 35.0 20.0 36.0 90-91 31.437711648668163 35.0 32.0 35.0 20.0 36.0 92-93 31.29445903596283 35.0 32.0 35.0 20.0 36.0 94-95 31.119805635315508 35.0 32.0 35.0 19.5 35.5 96-97 30.989242963583095 35.0 31.0 35.0 19.0 35.0 98-99 30.89120542848019 35.0 31.0 35.0 19.0 35.0 100 30.814526564000715 35.0 31.0 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5484898133292937 1101 2 0.5387543874464669 1101 3 0.5511573587778855 1101 4 0.44878691127337333 1101 5 0.4917201022558402 1101 6 0.4460717866871704 1101 7 0.48636092253025254 1101 8 0.4900869277070896 1101 9 0.5542767948246023 1101 10-11 0.5902198139513857 1101 12-13 0.5819394810707124 1101 14-15 0.7157680697177753 1101 16-17 0.6806282818489677 1101 18-19 0.684845848207253 1101 20-21 0.6906133196195796 1101 22-23 0.7595324279152393 1101 24-25 0.7455275950559894 1101 26-27 0.7074689130238028 1101 28-29 0.6968809236699158 1101 30-31 0.6765818096655494 1101 32-33 0.6213496970821168 1101 34-35 0.658492001487808 1101 36-37 0.6562645275276395 1101 38-39 0.6690792566581294 1101 40-41 0.6425223327972915 1101 42-43 0.6462318606292854 1101 44-45 0.6797852349036617 1101 46-47 0.6723372011085544 1101 48-49 0.7120468515615457 1101 50-51 0.618084616817768 1101 52-53 0.6572482336272216 1101 54-55 0.7235451547357741 1101 56-57 0.7478808408719644 1101 58-59 0.6965874929129896 1101 60-61 0.6985900284030322 1101 62-63 0.6602799673326771 1101 64-65 0.6443074998621015 1101 66-67 0.6269421820752044 1101 68-69 0.5998376826872018 1101 70-71 0.5725712231945863 1101 72-73 0.5255723563111374 1101 74-75 0.4955044993302309 1101 76-77 0.4994555791164146 1101 78-79 0.4985045519230624 1101 80-81 0.4639301475774644 1101 82-83 0.4614513170271053 1101 84-85 0.4542879723220139 1101 86-87 0.42670472875230914 1101 88-89 0.4167000000986718 1101 90-91 0.4371338227798027 1101 92-93 0.4328579549899665 1101 94-95 0.4455663634502507 1101 96-97 0.4364900625588426 1101 98-99 0.40058905468702477 1101 100 0.3715825935713717 1102 1 0.49650458550831544 1102 2 0.5334141482074628 1102 3 0.5066725145424513 1102 4 0.45586450087797203 1102 5 0.4710482068363575 1102 6 0.42973176340948527 1102 7 0.46096847392199436 1102 8 0.4845877262176046 1102 9 0.5519680347931342 1102 10-11 0.5541367793291272 1102 12-13 0.5405960468790383 1102 14-15 0.6240254902958355 1102 16-17 0.6155530835265139 1102 18-19 0.5789596047306702 1102 20-21 0.5819413477865183 1102 22-23 0.6121360707562005 1102 24-25 0.5979224228052047 1102 26-27 0.5893928521460836 1102 28-29 0.5812638016860703 1102 30-31 0.5823042420061455 1102 32-33 0.5623633406621735 1102 34-35 0.5713251631751461 1102 36-37 0.5895357662911636 1102 38-39 0.5978930625658236 1102 40-41 0.5663835466152349 1102 42-43 0.5730177320999701 1102 44-45 0.5677329979890899 1102 46-47 0.6150845716694846 1102 48-49 0.628787882982472 1102 50-51 0.5904217686294686 1102 52-53 0.6261373725486337 1102 54-55 0.673668363095338 1102 56-57 0.662131165231294 1102 58-59 0.5589006590561496 1102 60-61 0.5923272735197358 1102 62-63 0.5831355913493326 1102 64-65 0.5833759018530671 1102 66-67 0.5620108586845944 1102 68-69 0.5133370216755964 1102 70-71 0.5258418571753651 1102 72-73 0.511689206028926 1102 74-75 0.46549282899616884 1102 76-77 0.4736963054900869 1102 78-79 0.44447065669571373 1102 80-81 0.43216960223795553 1102 82-83 0.4105262005134662 1102 84-85 0.40412240640547736 1102 86-87 0.346612626865916 1102 88-89 0.33944926544319287 1102 90-91 0.33200170999973366 1102 92-93 0.306095432534466 1102 94-95 0.3867038091696706 1102 96-97 0.3701464026170278 1102 98-99 0.32077376611307784 1102 100 0.3000296313727979 1103 1 0.40848548146606234 1103 2 0.4315599912993875 1103 3 0.4430321264590873 1103 4 0.38964229082027657 1103 5 0.3946360101609727 1103 6 0.38367644212235774 1103 7 0.36352406291832295 1103 8 0.40715410979263567 1103 9 0.49878738740297734 1103 10-11 0.4479808818802198 1103 12-13 0.46435997089378844 1103 14-15 0.5474583489064813 1103 16-17 0.522009787600517 1103 18-19 0.5055412004541111 1103 20-21 0.4921773546979651 1103 22-23 0.5084324056669587 1103 24-25 0.5887467882250732 1103 26-27 0.5524305633617743 1103 28-29 0.5474452508370575 1103 30-31 0.4999620150314996 1103 32-33 0.46958455267166954 1103 34-35 0.48347842562120036 1103 36-37 0.5009721671861556 1103 38-39 0.5399444027694287 1103 40-41 0.5001853003680523 1103 42-43 0.5083677843907068 1103 44-45 0.4958258776604083 1103 46-47 0.5466214658447015 1103 48-49 0.5921446303861728 1103 50-51 0.5028605466657723 1103 52-53 0.4959318033212199 1103 54-55 0.583227166319233 1103 56-57 0.6081474515808125 1103 58-59 0.5804734600471022 1103 60-61 0.5934499515831462 1103 62-63 0.5539660425187094 1103 64-65 0.526718827661206 1103 66-67 0.48259907140556635 1103 68-69 0.5161774604060767 1103 70-71 0.47797314864811824 1103 72-73 0.4726908948853037 1103 74-75 0.48127662800938964 1103 76-77 0.47714467521853265 1103 78-79 0.4643491114988336 1103 80-81 0.40816002112942584 1103 82-83 0.398342567144212 1103 84-85 0.35573000293340584 1103 86-87 0.316027950716947 1103 88-89 0.3266569949180571 1103 90-91 0.34990197029339853 1103 92-93 0.3069757109422184 1103 94-95 0.39221990739732604 1103 96-97 0.376705997228413 1103 98-99 0.35368020975673886 1103 100 0.4041738215996169 1104 1 0.4160912018628018 1104 2 0.4325636031678499 1104 3 0.4166309129416277 1104 4 0.35694624249670426 1104 5 0.36078293818671625 1104 6 0.30290235240470054 1104 7 0.3128386406040633 1104 8 0.3620522960749426 1104 9 0.42893338814323556 1104 10-11 0.40545896728198727 1104 12-13 0.41936366330433117 1104 14-15 0.48528780686149986 1104 16-17 0.46263094470749166 1104 18-19 0.44419093779266206 1104 20-21 0.4040030810162776 1104 22-23 0.44992122536562107 1104 24-25 0.4770432325783531 1104 26-27 0.40421209477155173 1104 28-29 0.40810129355790536 1104 30-31 0.381898536535644 1104 32-33 0.35680759592244016 1104 34-35 0.3330537241483995 1104 36-37 0.34620149514483245 1104 38-39 0.34485654164124213 1104 40-41 0.3656981120672853 1104 42-43 0.33628935355432077 1104 44-45 0.3645523727732183 1104 46-47 0.3867383089488783 1104 48-49 0.4452719666472831 1104 50-51 0.4030451842795344 1104 52-53 0.3787367226198981 1104 54-55 0.527063225880056 1104 56-57 0.5542079088742966 1104 58-59 0.5138145694444631 1104 60-61 0.5484605097204991 1104 62-63 0.5412404712741363 1104 64-65 0.5317888087544489 1104 66-67 0.5197674572936037 1104 68-69 0.46483356987513247 1104 70-71 0.4622842432959402 1104 72-73 0.4533545011102973 1104 74-75 0.4411455684641439 1104 76-77 0.4377465414568107 1104 78-79 0.42086922100401125 1104 80-81 0.41031961339981393 1104 82-83 0.422400717080329 1104 84-85 0.40612587361093944 1104 86-87 0.3814854869901687 1104 88-89 0.3774683800720453 1104 90-91 0.35933477658337765 1104 92-93 0.3923379996232299 1104 94-95 0.46471261461212166 1104 96-97 0.4047525265629339 1104 98-99 0.35643501076119577 1104 100 0.2949081994539675 1105 1 0.23629496672262817 1105 2 0.3152578823288792 1105 3 0.30703044477996855 1105 4 0.2842560103831957 1105 5 0.2649577926786293 1105 6 0.2500063200418552 1105 7 0.2682724545167048 1105 8 0.2835508374343192 1105 9 0.28878033270238035 1105 10-11 0.29941593051248105 1105 12-13 0.2956840035896775 1105 14-15 0.3481730195948458 1105 16-17 0.31056853119176964 1105 18-19 0.3463598854789893 1105 20-21 0.32948471903977605 1105 22-23 0.37863452943967246 1105 24-25 0.4269559259766851 1105 26-27 0.40745714198121874 1105 28-29 0.3769962453835305 1105 30-31 0.3694373131524884 1105 32-33 0.3735160875071202 1105 34-35 0.4051223474969561 1105 36-37 0.3939328922016756 1105 38-39 0.422994756656891 1105 40-41 0.3920487509393311 1105 42-43 0.4141266914024513 1105 44-45 0.4246691030237315 1105 46-47 0.42065417548707984 1105 48-49 0.4449766084317943 1105 50-51 0.3136326333069519 1105 52-53 0.38014598012106404 1105 54-55 0.4738595855277907 1105 56-57 0.4915883407786552 1105 58-59 0.3942178925268962 1105 60-61 0.3824002792998442 1105 62-63 0.3970976780302351 1105 64-65 0.37592512044911786 1105 66-67 0.3715768691708874 1105 68-69 0.3664699478892075 1105 70-71 0.3474478901576248 1105 72-73 0.3233376035509181 1105 74-75 0.3384388071812978 1105 76-77 0.34668299368606625 1105 78-79 0.3650778498266334 1105 80-81 0.3072572453418658 1105 82-83 0.27853494533380996 1105 84-85 0.339833224483435 1105 86-87 0.2533680172552657 1105 88-89 0.2550887531988302 1105 90-91 0.24729234461743133 1105 92-93 0.23441404973354807 1105 94-95 0.2659476217810557 1105 96-97 0.30244220540150124 1105 98-99 0.24251789887146913 1105 100 0.2572832959114528 1106 1 0.2675678628568825 1106 2 0.31574145421691213 1106 3 0.2984149291487981 1106 4 0.2825569645780348 1106 5 0.30670075103704164 1106 6 0.2597729940855942 1106 7 0.2746848379404483 1106 8 0.292120079553591 1106 9 0.26426900163514944 1106 10-11 0.2486206115849825 1106 12-13 0.2787743659082409 1106 14-15 0.3605764268470537 1106 16-17 0.32957608844393604 1106 18-19 0.300406651390567 1106 20-21 0.32102065462247964 1106 22-23 0.3361472836298631 1106 24-25 0.3147335887218503 1106 26-27 0.3535216800355343 1106 28-29 0.32568731000887396 1106 30-31 0.3150462805541352 1106 32-33 0.3133872135805902 1106 34-35 0.33415448446523044 1106 36-37 0.2776620882142069 1106 38-39 0.2882839546134406 1106 40-41 0.3351755483869354 1106 42-43 0.34220125812969115 1106 44-45 0.3719484462451206 1106 46-47 0.3465739475181948 1106 48-49 0.35799567055776294 1106 50-51 0.2627107737489851 1106 52-53 0.3030753613805004 1106 54-55 0.43336553431199576 1106 56-57 0.45102216009166085 1106 58-59 0.42510752357910775 1106 60-61 0.4372346176176549 1106 62-63 0.3940583080894129 1106 64-65 0.38085132734449445 1106 66-67 0.37997348964515254 1106 68-69 0.3633436454307244 1106 70-71 0.330843898347716 1106 72-73 0.35589141557286297 1106 74-75 0.34745757553635315 1106 76-77 0.3576831700934875 1106 78-79 0.36831990807311854 1106 80-81 0.30513337570200605 1106 82-83 0.33943216103840257 1106 84-85 0.3441583619582005 1106 86-87 0.30583467876699544 1106 88-89 0.3204742557275182 1106 90-91 0.3157748422390476 1106 92-93 0.3275389976380616 1106 94-95 0.37646095113622735 1106 96-97 0.3566078870520819 1106 98-99 0.3457108718613924 1106 100 0.33321656756806206 1107 1 0.24141907930021844 1107 2 0.25763044822593173 1107 3 0.2506782597179651 1107 4 0.24783115737929506 1107 5 0.2374956811675517 1107 6 0.20256778763246075 1107 7 0.20783187428541794 1107 8 0.22786514336585384 1107 9 0.2609197030134567 1107 10-11 0.25549296704443947 1107 12-13 0.22520515800373886 1107 14-15 0.25187105344122784 1107 16-17 0.2719694801655024 1107 18-19 0.2576836292403826 1107 20-21 0.2637620285713993 1107 22-23 0.28013890634398564 1107 24-25 0.306869254970465 1107 26-27 0.26861796419291295 1107 28-29 0.3045432830915118 1107 30-31 0.2789896493322743 1107 32-33 0.20764268421680043 1107 34-35 0.29283320149566094 1107 36-37 0.24761397302007282 1107 38-39 0.2405346356206266 1107 40-41 0.2525835773733647 1107 42-43 0.30198029900699197 1107 44-45 0.2715364604368915 1107 46-47 0.2746292962869745 1107 48-49 0.3146154971174795 1107 50-51 0.2363086295059702 1107 52-53 0.24091361307332448 1107 54-55 0.313780661825696 1107 56-57 0.32925296865756337 1107 58-59 0.25236169771794437 1107 60-61 0.29096279204998154 1107 62-63 0.26270464314484343 1107 64-65 0.2099517753892286 1107 66-67 0.24993593398136937 1107 68-69 0.22982230059292164 1107 70-71 0.22461944844292248 1107 72-73 0.20292568526624422 1107 74-75 0.23924907137025997 1107 76-77 0.24549643483150874 1107 78-79 0.25921909847563995 1107 80-81 0.18960113994284455 1107 82-83 0.2473604074708433 1107 84-85 0.24818636177460718 1107 86-87 0.21041559740302418 1107 88-89 0.22405101695612473 1107 90-91 0.26066423500595803 1107 92-93 0.2693913738994418 1107 94-95 0.27559197107985156 1107 96-97 0.27042075330863113 1107 98-99 0.23519036704434626 1107 100 0.25557384582342735 1108 1 0.11368496570986864 1108 2 0.1573605967680649 1108 3 0.1853646121454915 1108 4 0.16742334754853516 1108 5 0.1601764736108109 1108 6 0.09191327604058586 1108 7 0.1304925913242556 1108 8 0.15852144558893855 1108 9 0.19381546397254112 1108 10-11 0.142630128059821 1108 12-13 0.1411920771101265 1108 14-15 0.20951765410064382 1108 16-17 0.17233891802198542 1108 18-19 0.17230542581314978 1108 20-21 0.14088633669621942 1108 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-1.0679081692587395 2316 94-95 -1.2691985671665726 2316 96-97 -1.3728436934637323 2316 98-99 -1.2736453112109913 2316 100 -1.0779221588580086 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14114.0 3 3586.0 4 2490.0 5 2107.0 6 2596.0 7 7977.0 8 15820.0 9 20466.0 10 23474.0 11 25275.0 12 29229.0 13 34334.0 14 41458.0 15 51418.0 16 60509.0 17 71376.0 18 83611.0 19 97526.0 20 115491.0 21 140218.0 22 177254.0 23 248727.0 24 356369.0 25 439067.0 26 439857.0 27 394607.0 28 376625.0 29 381283.0 30 428942.0 31 520515.0 32 649364.0 33 842924.0 34 1165891.0 35 1771424.0 36 3121709.0 37 5967950.0 38 7336691.0 39 2098565.0 40 3119.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 12.08560017997808 26.254229528586237 32.219034139009175 29.441136152426512 2 21.334672371235207 34.00286442423343 26.391905315573783 18.27055788895758 3 24.076794890486862 24.854773008646244 27.809640603988456 23.258791496878437 4 18.027840997965388 29.28797989113464 23.139085349579208 29.545093761320757 5 23.723182067759286 31.38616872930959 26.098874803975527 18.7917743989556 6 29.80288538483728 28.89896054254012 24.240949248373216 17.057204824249386 7 22.4364578771801 25.04252465252794 30.995594554257867 21.525422916034092 8 27.573009921396498 26.995996890810144 24.495852930015435 20.935140257777928 9 25.06323025490636 24.57885095511552 28.378500568991466 21.97941822098666 10-11 21.575738342295008 29.44393520131535 27.89697978198824 21.083346674401398 12-13 21.233381641848702 26.97730880228521 28.187982390387294 23.60132716547879 14-15 21.125573776876344 31.532542469334263 26.152614513054584 21.189269240734813 16-17 23.628328361426735 26.4110023147895 26.267069587957444 23.693599735826325 18-19 20.9204172502874 28.92039297798722 26.500037689286472 23.659152082438904 20-21 23.34514509521553 29.308428849786395 26.38347380235619 20.96295225264189 22-23 23.453568548849454 26.79512355856093 28.650801171764556 21.100506720825056 24-25 21.134933564228607 28.799264696750594 26.22016552675199 23.845636212268808 26-27 21.24911833548639 26.392732177676898 31.25975268603455 21.098396800802163 28-29 21.16848382752679 26.54624511667487 31.238985429726984 21.046285626071363 30-31 20.95009925050376 26.354889629630208 31.411857913466545 21.283153206399483 32-33 21.258085466314718 26.676603101255647 30.94715693415772 21.118154498271913 34-35 21.302862866848656 26.83893953894921 30.71982036395482 21.138377230247315 36-37 21.09699701957161 26.770044715504724 30.965881049421657 21.167077215502005 38-39 21.130583471935473 26.760171839237024 31.05926862337864 21.049976065448863 40-41 21.150791996407932 26.72331650556743 31.022026757760212 21.103864740264427 42-43 21.08053194604595 26.75968218194421 31.039502981783667 21.120282890226168 44-45 21.153804125744326 26.86567058520119 30.941681146860383 21.038844142194097 46-47 21.132417950854887 26.904412787928507 30.916737145991736 21.04643211522487 48-49 21.179812404359915 26.955302586696916 30.833303618380704 21.03158139056247 50-51 21.213740528427447 27.026600768119142 30.794782661241793 20.964876042211618 52-53 21.299419253021707 27.052882361562137 30.618265292616687 21.029433092799472 54-55 21.4762573422579 27.270818447617494 30.069903212612587 21.18302099751202 56-57 21.90246775801082 27.72072909247611 29.1683005159786 21.208502633534472 58-59 21.984619502201298 28.085084097836976 28.64241690911588 21.28787949084585 60-61 22.07786823931384 28.262249409609964 28.441611259048965 21.218271092027233 62-63 22.079008118744618 28.426607028797623 28.283127195021546 21.211257657436214 64-65 22.188548902549105 28.43546798115439 28.209902402802694 21.166080713493816 66-67 22.16731085134175 28.321432899820504 28.41447133737047 21.09678491146728 68-69 22.274809949491445 28.229147697760038 28.509336271077757 20.98670608167076 70-71 22.340240098980637 28.120598111342243 28.55637640444285 20.98278538523427 72-73 22.40897193304447 28.060454161678955 28.61509540922705 20.915478496049527 74-75 22.625950482218578 28.187106133196497 28.423908813416617 20.763034571168305 76-77 22.874810491599813 28.265919164160515 28.154048757315405 20.70522158692427 78-79 23.061606404946485 28.405981964613858 27.88491086817519 20.647500762264468 80-81 23.341325137072197 28.551131821325544 27.596464435371722 20.511078606230544 82-83 23.54840393825223 28.621454640049066 27.265240933525764 20.564900488172942 84-85 23.66590879010415 28.67436471086134 27.161813292862885 20.497913206171628 86-87 23.83200331584113 28.754917637964496 26.980675635324314 20.43240341087006 88-89 24.091742085728104 28.779517134546307 26.76493391508108 20.363806864644506 90-91 24.28589871874011 28.762143790427945 26.69155285037868 20.260404640453267 92-93 24.551931635527733 28.772132933953955 26.598098813480515 20.077836617037796 94-95 24.750929393823494 28.76015682580234 26.43279205432694 20.056121726047227 96-97 24.87621686211462 28.7497240121524 26.37322435103896 20.00083477469402 98-99 25.101692740102948 28.779049989678434 26.24455847626329 19.87469879395532 100 25.15382688065943 28.72194363399238 26.153250300004277 19.97097918534391 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 34087.0 1 23812.5 2 11577.0 3 8934.5 4 8178.0 5 8148.0 6 8202.0 7 8298.0 8 8288.5 9 8252.0 10 8837.5 11 11196.0 12 88212.5 13 109527.0 14 54445.5 15 56637.5 16 64037.5 17 71159.5 18 77493.0 19 83750.0 20 91706.5 21 105382.5 22 125442.5 23 150337.0 24 176451.0 25 205105.5 26 238116.0 27 278350.5 28 334405.0 29 393827.0 30 438760.5 31 479843.0 32 524908.0 33 556669.0 34 582851.0 35 625123.0 36 674573.0 37 719156.5 38 762457.0 39 790056.0 40 801400.0 41 827852.5 42 875144.5 43 897200.0 44 900655.0 45 916026.5 46 930948.0 47 948025.5 48 949780.5 49 942138.5 50 947177.0 51 946737.0 52 932798.0 53 901844.0 54 846858.0 55 784078.5 56 722516.5 57 656481.5 58 583751.0 59 502550.5 60 419990.0 61 343782.0 62 269705.0 63 199834.5 64 142809.5 65 101214.0 66 71724.5 67 51714.5 68 37297.5 69 27250.5 70 20498.0 71 15734.5 72 12658.0 73 10189.5 74 8319.0 75 7314.0 76 5828.0 77 3572.5 78 2129.0 79 1535.0 80 1200.5 81 994.0 82 832.0 83 673.5 84 590.0 85 513.0 86 418.0 87 366.5 88 295.5 89 242.0 90 237.5 91 194.5 92 155.5 93 137.5 94 113.5 95 95.0 96 85.0 97 71.0 98 51.0 99 40.5 100 28.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4053336607173759 2 0.38613104837846585 3 0.16813985857909086 4 0.1746265902741544 5 0.27456506790497937 6 0.10212974493721111 7 0.13630843582042898 8 0.14186279053247725 9 0.13489717260489223 10-11 0.131247479044918 12-13 0.13321744286506315 14-15 0.13949375485189755 16-17 0.1438146147226026 18-19 0.15554551345637663 20-21 0.1368562526470255 22-23 0.0986705174924443 24-25 0.09407937713444492 26-27 0.08862841831350926 28-29 0.0984510279692053 30-31 0.10277732972891629 32-33 0.10374054408296515 34-35 0.10421943031548662 36-37 0.100304898157224 38-39 0.09942875402726996 40-41 0.10057336468151634 42-43 0.06328009932390696 44-45 0.06634025490823924 46-47 0.0848045843053454 48-49 0.08892772220883517 50-51 0.09825874789099592 52-53 0.10329612314748121 54-55 0.10583385738724461 56-57 0.10625832472970682 58-59 0.10576674075617153 60-61 0.10361175270982492 62-63 0.10802149676762676 64-65 0.11467874098487597 66-67 0.12312636668507476 68-69 0.12826532386967068 70-71 0.12674703683701738 72-73 0.11353957221963552 74-75 0.11136644454326915 76-77 0.11321850076828589 78-79 0.11196868025992494 80-81 0.10626013869270878 82-83 0.1094563415021892 84-85 0.11525920914550805 86-87 0.1287841172882356 88-89 0.13639006415551788 90-91 0.14311623896684214 92-93 0.14240335150706585 94-95 0.13231953117908538 96-97 0.12905258381252793 98-99 0.12412948822516708 100 0.11857513351311882 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.7563958E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.634610333151585 #Duplication Level Percentage of deduplicated Percentage of total 1 68.84090609568258 24.531188637007283 2 14.98599801565379 10.680403994824115 3 5.570940030492101 5.955548315278249 4 2.730634729711976 3.8922041822182782 5 1.6705747079814708 2.97651393756691 6 1.1069484268035774 2.366740550882439 7 0.7933812585639402 1.9790282396185976 8 0.6166957839648012 1.7580571164546495 9 0.4402229599874909 1.4118456274974738 >10 2.8199562817668817 18.844770498783532 >50 0.23837454337951053 5.845353678147321 >100 0.17507216105220533 12.574374308914372 >500 0.009321818452006138 2.092622167088259 >1k 9.324544268942494E-4 0.5182871790513005 >5k 0.0 0.0 >10k+ 4.0732080774821154E-5 4.573061566667375 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1163681 4.221748560203147 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 46208 0.16763920479054567 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 37779 0.13705941650324674 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.001385867733509099 3.6279260039505213E-6 0.0 0.0 0.0 2 0.0014185190675446537 1.0883778011851564E-5 0.0 0.0 3.6279260039505213E-6 3 0.0014765658836078622 1.0883778011851564E-5 0.0 0.0 7.255852007901043E-6 4 0.0015092172176434167 2.539548202765365E-5 0.0 3.6279260039505213E-6 1.4511704015802085E-5 5 0.00153098477366712 5.4418890059257816E-5 0.0 3.6279260039505213E-6 2.539548202765365E-5 6 0.0015708919597105756 5.4418890059257816E-5 0.0 3.6279260039505213E-6 2.539548202765365E-5 7 0.0015781478117184767 6.167474206715887E-5 0.0 3.6279260039505213E-6 2.539548202765365E-5 8 0.0015962874417382294 6.167474206715887E-5 0.0 3.6279260039505213E-6 2.902340803160417E-5 9 0.0016579621838053882 6.89305940750599E-5 0.0 3.6279260039505213E-6 2.902340803160417E-5 10-11 0.0017141950368666213 7.981437208691147E-5 0.0 5.441889005925782E-6 3.083737103357943E-5 12-13 0.0017831256309416813 8.162833508888673E-5 0.0 7.255852007901043E-6 3.990718604345573E-5 14-15 0.0018919634110601967 8.707022409481251E-5 0.0 7.255852007901043E-6 4.716303805135677E-5 16-17 0.0019645219311392073 9.069815009876303E-5 0.0 1.0883778011851564E-5 5.986077906518361E-5 18-19 0.0020642898962478467 9.614003910468882E-5 0.0 1.4511704015802085E-5 6.167474206715887E-5 20-21 0.0021132668973011786 1.0520985411456511E-4 0.0 1.4511704015802085E-5 8.344229809086199E-5 22-23 0.0021894533433841398 1.197215581303672E-4 0.0 1.4511704015802085E-5 1.0883778011851563E-4 24-25 0.0024524779786705524 1.197215581303672E-4 0.0 1.4511704015802085E-5 1.1609363212641667E-4 26-27 0.0030492718063204133 1.2153552113234247E-4 0.0 1.8139630019752605E-5 1.3060533614221877E-4 28-29 0.004718117768137653 1.3060533614221877E-4 0.0 1.9953593021727865E-5 1.378611881501198E-4 30-31 0.008413160403161257 1.342332621461693E-4 0.0 2.1767556023703127E-5 1.5237289216592188E-4 32-33 0.01625129453469636 1.378611881501198E-4 0.0 2.1767556023703127E-5 1.7776837419357554E-4 34-35 0.030594299991314747 1.4148911415407032E-4 0.0 2.358151902567839E-5 1.9228007820937764E-4 36-37 0.054500518394346706 1.4511704015802084E-4 0.0 2.539548202765365E-5 2.067917822251797E-4 38-39 0.09046052094550427 1.4874496616197136E-4 0.0 2.539548202765365E-5 2.1041970822913023E-4 40-41 0.13979668667322742 1.5055892916394662E-4 0.0 2.539548202765365E-5 2.15861597235056E-4 42-43 0.2050612615212953 1.6325667017777346E-4 0.0 2.539548202765365E-5 2.267453752469076E-4 44-45 0.2891184930698269 1.795823371955508E-4 0.0 2.539548202765365E-5 2.2855933824888285E-4 46-47 0.39987363208143034 1.9590800421332815E-4 0.0 2.902340803160417E-5 2.303733012508581E-4 48-49 0.5392277117821759 1.977219672153034E-4 0.0 2.902340803160417E-5 2.4125707926270966E-4 50-51 0.7077049674796341 2.0860574522715497E-4 0.0 3.627926003950521E-5 2.4669896826863547E-4 52-53 0.8998109052408221 2.1404763423308075E-4 0.0 4.3535112047406254E-5 2.557687832785118E-4 54-55 1.1193294518878603 2.1767556023703126E-4 0.0 4.5349075049381513E-5 2.684665242923386E-4 56-57 1.37876062646736 2.394431162607344E-4 0.0 4.716303805135677E-5 2.720944502962891E-4 58-59 1.6832198046448918 2.57582746280487E-4 0.0 4.716303805135677E-5 2.9204804331801697E-4 60-61 2.0300295770295396 2.666525612903633E-4 0.0 4.716303805135677E-5 3.0655974733381905E-4 62-63 2.4126524209621856 2.7028048729431386E-4 0.0 5.260492705728256E-5 3.192574883476459E-4 64-65 2.8246632794898323 2.757223763002396E-4 0.0 5.4418890059257816E-5 3.3014126635949744E-4 66-67 3.2838135945498106 2.8297822830814065E-4 0.0 5.804681606320834E-5 3.4828089637925003E-4 68-69 3.788732010112626 2.902340803160417E-4 0.0 5.804681606320834E-5 3.7911826741282947E-4 70-71 4.340164064972091 2.902340803160417E-4 0.0 5.804681606320834E-5 3.936299714286316E-4 72-73 4.929712924392064 2.99303895325918E-4 0.0 6.711663107308464E-5 4.135835644503594E-4 74-75 5.552849122756609 3.083737103357943E-4 0.0 6.89305940750599E-5 4.31723194470112E-4 76-77 6.213318493664807 3.120016363397448E-4 0.0 6.89305940750599E-5 4.462348984859141E-4 78-79 6.91329960668203 3.265133403555469E-4 0.0 6.89305940750599E-5 4.553047134957904E-4 80-81 7.64507042130887 3.3921108136937377E-4 0.0 7.074455707703516E-5 4.7707226951949357E-4 82-83 8.412888308710961 3.5190882238320055E-4 0.0 7.618644608296094E-5 4.825141585254193E-4 84-85 9.211151751138207 3.682344894009779E-4 0.0 7.981437208691146E-5 5.060956775510977E-4 86-87 10.037270409423785 3.791182674128294E-4 0.0 8.344229809086199E-5 5.242353075708504E-4 88 10.673532444070624 3.8456015641875527E-4 0.0 8.344229809086199E-5 5.296771965767761E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 152075 0.0 82.66236 8 AGTGGTA 154365 0.0 81.63549 5 GTGGTAT 154555 0.0 81.52131 6 TGGTATC 154155 0.0 81.385704 7 GTATCAA 154715 0.0 81.14761 9 AAGCAGT 161515 0.0 78.26692 1 GCAGTGG 163090 0.0 77.633415 3 CAGTGGT 168515 0.0 74.645294 4 AGCAGTG 174190 0.0 72.76294 2 CAACGCA 149785 0.0 44.313854 12-13 ATCAACG 150720 0.0 44.130547 10-11 ACGCAGA 152060 0.0 43.7106 14-15 AGAGTAC 151800 0.0 43.06796 18-19 TCAACGC 149395 0.0 42.21121 12-13 GAGTACT 147085 0.0 41.9742 20-21 AACGCAG 150980 0.0 41.877777 14-15 CGCAGAG 153820 0.0 41.06649 16-17 AGTACTT 158005 0.0 40.862736 20-21 TATCAAC 154380 0.0 40.648624 10-11 GTACTTT 158150 0.0 39.0212 22-23 >>END_MODULE