FastQCFastQC Report
Thu 26 May 2016
SRR1660715_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660715_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27563958
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11472054.161974851362057No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT503580.18269509770694034No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA325440.11806722387256575No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1588900.082.0250248
AGTGGTA1590200.081.2182165
GTGGTAT1598150.080.852396
TGGTATC1612250.080.839737
GTATCAA1612450.080.73659
AAGCAGT1581450.079.4884641
GCAGTGG1641600.078.5868453
CAGTGGT1726050.074.712184
AGCAGTG1745050.073.703492
CAACGCA1555650.044.29992712-13
ATCAACG1565600.044.04986610-11
ACGCAGA1580000.043.6798314-15
AGAGTAC1591800.043.12656418-19
GAGTACT1534400.041.9665920-21
TCAACGC1553150.041.95493712-13
AACGCAG1566050.041.66802214-15
AGTACTT1646600.041.07835420-21
CGCAGAG1597200.040.88208416-17
TATCAAC1608100.040.51255410-11
GTACTTT1642050.039.1644722-23
Produced by FastQC (version 0.11.5)