##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660715_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 27563958 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.18256822913458 34.0 31.0 34.0 27.0 34.0 2 31.84950125812846 34.0 31.0 34.0 28.0 34.0 3 31.64590390102902 34.0 31.0 34.0 30.0 34.0 4 35.573280803867135 37.0 35.0 37.0 33.0 37.0 5 35.91110318772072 37.0 35.0 37.0 35.0 37.0 6 35.33940230934904 37.0 35.0 37.0 33.0 37.0 7 35.557821413020584 37.0 35.0 37.0 33.0 37.0 8 35.921715850822295 37.0 35.0 37.0 33.0 37.0 9 37.71617091420615 39.0 37.0 39.0 35.0 39.0 10-11 37.77008359975008 39.0 37.5 39.0 35.0 39.0 12-13 37.819852341234885 39.0 38.0 39.0 35.0 39.0 14-15 39.25953152301277 41.0 39.0 41.0 36.0 41.0 16-17 39.22796653513984 41.0 39.0 41.0 36.0 41.0 18-19 39.22723541735189 41.0 39.0 41.0 36.0 41.0 20-21 39.17613058327835 40.5 39.0 41.0 36.0 41.0 22-23 39.076191198666024 40.0 39.0 41.0 35.5 41.0 24-25 39.07320225926915 40.0 39.0 41.0 35.5 41.0 26-27 39.00384678064014 40.0 39.0 41.0 35.0 41.0 28-29 38.91646388374268 40.0 38.5 41.0 35.0 41.0 30-31 38.82402565335501 40.0 38.0 41.0 35.0 41.0 32-33 38.70794638781557 40.0 38.0 41.0 35.0 41.0 34-35 38.62508234485047 40.0 38.0 41.0 34.0 41.0 36-37 38.51350707688641 40.0 38.0 41.0 34.0 41.0 38-39 38.18230750823231 40.0 37.0 41.0 34.0 41.0 40-41 38.0564273461743 40.0 37.0 41.0 33.5 41.0 42-43 37.89389448351358 40.0 37.0 41.0 33.0 41.0 44-45 37.77234385206943 40.0 37.0 41.0 33.0 41.0 46-47 37.62105703397168 40.0 36.0 41.0 33.0 41.0 48-49 37.66810740678099 40.0 36.0 41.0 33.0 41.0 50-51 37.67923860934631 40.0 36.0 41.0 33.0 41.0 52-53 37.4605523814831 40.0 36.0 41.0 32.5 41.0 54-55 37.0243766152887 40.0 36.0 41.0 31.0 41.0 56-57 36.62121637248177 39.0 35.5 41.0 30.5 41.0 58-59 36.48860595419569 39.0 35.0 41.0 30.0 41.0 60-61 36.32768258462736 39.0 35.0 41.0 30.0 41.0 62-63 36.08455855650339 39.0 35.0 41.0 29.5 41.0 64-65 35.808827600158146 38.0 35.0 40.0 29.0 41.0 66-67 35.51459603515576 37.0 35.0 40.0 29.0 41.0 68-69 35.23468879179107 37.0 35.0 40.0 29.0 41.0 70-71 34.92662080677964 36.0 34.5 39.0 29.0 41.0 72-73 34.58125567452976 36.0 34.0 39.0 28.5 41.0 74-75 34.20706416690956 35.0 34.0 38.5 28.0 40.0 76-77 32.51214250507856 34.5 32.0 36.0 25.5 39.0 78-79 33.454561859367224 35.0 33.5 37.0 27.0 39.0 80-81 33.36225856968727 35.0 34.0 36.5 27.0 39.0 82-83 33.115181317574205 35.0 34.0 36.0 27.0 37.5 84-85 32.87861997177619 35.0 34.0 36.0 26.5 37.0 86-87 32.678073791144215 35.0 34.0 35.0 26.0 37.0 88-89 32.516939022327634 35.0 34.0 35.0 26.0 36.0 90-91 32.36775933993224 35.0 33.0 35.0 25.5 36.0 92-93 32.23845336362797 35.0 33.0 35.0 25.0 36.0 94-95 32.14384606884106 35.0 33.0 35.0 25.0 36.0 96-97 32.05236994629001 35.0 33.0 35.0 25.0 35.0 98-99 31.967387865704918 35.0 33.0 35.0 25.0 35.0 100 31.9181593224021 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.6582533242690083 1101 2 1.2564588855833314 1101 3 1.542739612665489 1101 4 0.9559915775540802 1101 5 0.5750018624039726 1101 6 1.1201932510663113 1101 7 0.8136703090769188 1101 8 0.5061851437245863 1101 9 0.5073759772889801 1101 10-11 0.4124314428879856 1101 12-13 0.3740825784361945 1101 14-15 0.5067094111845236 1101 16-17 0.5544970297593039 1101 18-19 0.5158251757332692 1101 20-21 0.5094301401874262 1101 22-23 0.513285440631762 1101 24-25 0.5057951123706843 1101 26-27 0.5053868428682193 1101 28-29 0.49210849153745784 1101 30-31 0.5036233377746626 1101 32-33 0.5454422803894943 1101 34-35 0.5299536910194149 1101 36-37 0.5494357345015715 1101 38-39 0.5448548783203364 1101 40-41 0.5344529801036089 1101 42-43 0.5116040668410449 1101 44-45 0.5080232443704915 1101 46-47 0.5193962271535284 1101 48-49 0.49587014626761317 1101 50-51 0.487015952848445 1101 52-53 0.4528513630152773 1101 54-55 0.4784406824366201 1101 56-57 0.5066671032153138 1101 58-59 0.4965532440993883 1101 60-61 0.5067646051242036 1101 62-63 0.509173465130992 1101 64-65 0.4748257470559025 1101 66-67 0.499836198187964 1101 68-69 0.4600939135450304 1101 70-71 0.4443150733475747 1101 72-73 0.4092564961748266 1101 74-75 0.4262719804823618 1101 76-77 0.36497952414878654 1101 78-79 0.45488786086573185 1101 80-81 0.47543173612334044 1101 82-83 0.4471018470348511 1101 84-85 0.45169698447809026 1101 86-87 0.4498227141159674 1101 88-89 0.4410861169389193 1101 90-91 0.4323668330322903 1101 92-93 0.4133189271091524 1101 94-95 0.3682422180357534 1101 96-97 0.3747487422195732 1101 98-99 0.38196460802189236 1101 100 0.40726951074335105 1102 1 1.5638019707318342 1102 2 1.1825817250785455 1102 3 1.499811317589792 1102 4 0.9193029861849169 1102 5 0.506024348939782 1102 6 1.0853863535684098 1102 7 0.7990489121820588 1102 8 0.46365625187932125 1102 9 0.47485007445153826 1102 10-11 0.3839672063400812 1102 12-13 0.3795547183639485 1102 14-15 0.49645442620393254 1102 16-17 0.49218560511728526 1102 18-19 0.4531153988320895 1102 20-21 0.43508488201045026 1102 22-23 0.44456068955260264 1102 24-25 0.44337684530621146 1102 26-27 0.4429884209490069 1102 28-29 0.47444441708953633 1102 30-31 0.4963287339847611 1102 32-33 0.5050016608734182 1102 34-35 0.47567849354685166 1102 36-37 0.46777779355235083 1102 38-39 0.4914593912289149 1102 40-41 0.4628670071629699 1102 42-43 0.4952628351361028 1102 44-45 0.45913493674079575 1102 46-47 0.46720014679831223 1102 48-49 0.45270120722651797 1102 50-51 0.4126334990149161 1102 52-53 0.4099701093378272 1102 54-55 0.4505984786022168 1102 56-57 0.4913013246155842 1102 58-59 0.48268343963467686 1102 60-61 0.4917537759465702 1102 62-63 0.46459505030527026 1102 64-65 0.4240283690812561 1102 66-67 0.43678937252337846 1102 68-69 0.41405118272252395 1102 70-71 0.3933133668282309 1102 72-73 0.38414571545569487 1102 74-75 0.3970505210300459 1102 76-77 0.21172590300260907 1102 78-79 0.43338253005109806 1102 80-81 0.4603799797720214 1102 82-83 0.44135169295563514 1102 84-85 0.4261436253386677 1102 86-87 0.40617904148661665 1102 88-89 0.3826884163578228 1102 90-91 0.42360795308386656 1102 92-93 0.4191264369021539 1102 94-95 0.38167517509860716 1102 96-97 0.3878371647200396 1102 98-99 0.3988629550451712 1102 100 0.38903821344110767 1103 1 1.5269860353655709 1103 2 1.1272203867021808 1103 3 1.4137523659851219 1103 4 0.8867516909090654 1103 5 0.5018010199634801 1103 6 1.04750167360654 1103 7 0.775363799245504 1103 8 0.43056236623961297 1103 9 0.43998209409051015 1103 10-11 0.3515493941848149 1103 12-13 0.32983100921887143 1103 14-15 0.3830798312284216 1103 16-17 0.41023208828615765 1103 18-19 0.38194944818268084 1103 20-21 0.3860075088660224 1103 22-23 0.3764840115619421 1103 24-25 0.3848262077910576 1103 26-27 0.3816723700391975 1103 28-29 0.35129578703939757 1103 30-31 0.3459796317655517 1103 32-33 0.3883921205713534 1103 34-35 0.39594594673909 1103 36-37 0.37449819854425215 1103 38-39 0.380657537177143 1103 40-41 0.40001647598152346 1103 42-43 0.41241783686838573 1103 44-45 0.3805412570608979 1103 46-47 0.379491421938674 1103 48-49 0.35258960711614407 1103 50-51 0.34689152014527025 1103 52-53 0.3627334305425265 1103 54-55 0.3803196182436679 1103 56-57 0.4041372612827132 1103 58-59 0.42451350621958994 1103 60-61 0.40908921329171477 1103 62-63 0.3610859290930293 1103 64-65 0.3217301939194641 1103 66-67 0.3543671078935233 1103 68-69 0.3672124633376299 1103 70-71 0.34853644281425744 1103 72-73 0.30931076015532 1103 74-75 0.3513874678359272 1103 76-77 0.24140408879285502 1103 78-79 0.4105949928866792 1103 80-81 0.43290437936782666 1103 82-83 0.4197289475005732 1103 84-85 0.3799642016167937 1103 86-87 0.37062511895287287 1103 88-89 0.3549478840780722 1103 90-91 0.3252504141875363 1103 92-93 0.3292820977618831 1103 94-95 0.3322909360386106 1103 96-97 0.340072318354423 1103 98-99 0.3653033608893672 1103 100 0.3500999038170427 1104 1 1.4898009172462174 1104 2 1.1041987471141788 1104 3 1.3969211508969046 1104 4 0.8598622632635227 1104 5 0.4744434151374861 1104 6 1.0177396317788805 1104 7 0.7658242111065761 1104 8 0.41465436057806215 1104 9 0.4310275729934716 1104 10-11 0.30361340219759825 1104 12-13 0.29673464170652153 1104 14-15 0.36905763157849236 1104 16-17 0.3886063418112826 1104 18-19 0.34290227069332246 1104 20-21 0.3530983305731681 1104 22-23 0.3534342247940998 1104 24-25 0.3523916403197518 1104 26-27 0.35201224950706234 1104 28-29 0.33787818289790295 1104 30-31 0.33075426811927144 1104 32-33 0.38178013403818056 1104 34-35 0.38210397200715107 1104 36-37 0.37477416905375094 1104 38-39 0.3875735617184546 1104 40-41 0.38734752767891223 1104 42-43 0.4064309041980607 1104 44-45 0.35373892425680253 1104 46-47 0.37223914846800454 1104 48-49 0.3245405476980423 1104 50-51 0.3329943583759416 1104 52-53 0.3669609849848996 1104 54-55 0.38960166851772726 1104 56-57 0.3938956275232286 1104 58-59 0.39937760557298674 1104 60-61 0.410547582218733 1104 62-63 0.3436263759620459 1104 64-65 0.3289772380031053 1104 66-67 0.32379602748225267 1104 68-69 0.3141398628061367 1104 70-71 0.32798434716443126 1104 72-73 0.32045950363103515 1104 74-75 0.33962521770024523 1104 76-77 0.21932229639543266 1104 78-79 0.36198356305076373 1104 80-81 0.3735116522304267 1104 82-83 0.35976646196985484 1104 84-85 0.36795960561811114 1104 86-87 0.39220370894137346 1104 88-89 0.34585818915843447 1104 90-91 0.3776611497829805 1104 92-93 0.37150609008971713 1104 94-95 0.36304656715362427 1104 96-97 0.3382316069753486 1104 98-99 0.35645563232020194 1104 100 0.3854254712921126 1105 1 1.376025587234043 1105 2 1.0254433706890858 1105 3 1.3341612059569137 1105 4 0.8243205939431562 1105 5 0.4308508825665669 1105 6 0.9833247392989222 1105 7 0.7157496095923861 1105 8 0.3527497198274361 1105 9 0.3382055790277434 1105 10-11 0.2685555012162055 1105 12-13 0.2552556906952006 1105 14-15 0.31440888583208704 1105 16-17 0.29404635166739723 1105 18-19 0.27670826363502243 1105 20-21 0.2767736523915332 1105 22-23 0.2789641191543666 1105 24-25 0.28293746872235914 1105 26-27 0.3032408933243147 1105 28-29 0.2985564154667699 1105 30-31 0.264194011906703 1105 32-33 0.3174065969973867 1105 34-35 0.32773960176170647 1105 36-37 0.31355702645657857 1105 38-39 0.3726574486854588 1105 40-41 0.3585751381643689 1105 42-43 0.3442673717074598 1105 44-45 0.31188995050777635 1105 46-47 0.33588112266263437 1105 48-49 0.34361002396384777 1105 50-51 0.32835506981374607 1105 52-53 0.3059348648151641 1105 54-55 0.32684800598480024 1105 56-57 0.3601124112965479 1105 58-59 0.33310555133478914 1105 60-61 0.31218865134201934 1105 62-63 0.29441712312991086 1105 64-65 0.299490068315599 1105 66-67 0.3274471591820003 1105 68-69 0.2774768850070046 1105 70-71 0.2977858098559665 1105 72-73 0.2603125548186185 1105 74-75 0.3109282216695135 1105 76-77 0.20199131991216035 1105 78-79 0.29076681064349685 1105 80-81 0.3058075104261846 1105 82-83 0.3007993087303049 1105 84-85 0.29295570463884957 1105 86-87 0.3187546807129209 1105 88-89 0.3287525146933774 1105 90-91 0.3025117738274474 1105 92-93 0.2806716892071037 1105 94-95 0.2930640777765987 1105 96-97 0.31123854139140406 1105 98-99 0.3137857443516161 1105 100 0.2806449154946442 1106 1 1.394340159005349 1106 2 1.0301047297445827 1106 3 1.3251757246719542 1106 4 0.8109940321248672 1106 5 0.42917185933794855 1106 6 0.940900321773384 1106 7 0.6694477150294418 1106 8 0.32361716041216937 1106 9 0.336016581829675 1106 10-11 0.22849956043019404 1106 12-13 0.20458953392693502 1106 14-15 0.25059069104955967 1106 16-17 0.24580523591646397 1106 18-19 0.20657213175977063 1106 20-21 0.23198862841640278 1106 22-23 0.2504329564052128 1106 24-25 0.24903257695338255 1106 26-27 0.2067324928767036 1106 28-29 0.2253175021707321 1106 30-31 0.24647720287925523 1106 32-33 0.30653194312331067 1106 34-35 0.31772350818447137 1106 36-37 0.29243425695478464 1106 38-39 0.3161665508672016 1106 40-41 0.29894972466722436 1106 42-43 0.32728510905238295 1106 44-45 0.32294298383658315 1106 46-47 0.3045275081104606 1106 48-49 0.2722964178619307 1106 50-51 0.26908280554420827 1106 52-53 0.2525273589435457 1106 54-55 0.2429025046890061 1106 56-57 0.26491777676643125 1106 58-59 0.26172037329104825 1106 60-61 0.26314146647065684 1106 62-63 0.23560853008685 1106 64-65 0.19982748680209284 1106 66-67 0.1839954487315083 1106 68-69 0.18451884371496874 1106 70-71 0.19729387916249408 1106 72-73 0.14849800395681 1106 74-75 0.1736642537963462 1106 76-77 0.05148395620681612 1106 78-79 0.20429097869473622 1106 80-81 0.23096502074378122 1106 82-83 0.21205034326811756 1106 84-85 0.2151268060281737 1106 86-87 0.24152899822482254 1106 88-89 0.22244833602813685 1106 90-91 0.20623862582110064 1106 92-93 0.23358012415935292 1106 94-95 0.22440762742642306 1106 96-97 0.23659855393390217 1106 98-99 0.27244239837825646 1106 100 0.2684858073463481 1107 1 1.344525863638669 1107 2 0.9584192015047783 1107 3 1.2909365797516692 1107 4 0.7836197009017667 1107 5 0.3778968103346969 1107 6 0.9352170756402742 1107 7 0.6448808117918077 1107 8 0.2812906070438359 1107 9 0.27422638259415066 1107 10-11 0.21122055046416222 1107 12-13 0.16283556776266295 1107 14-15 0.1742453922915317 1107 16-17 0.15988909064891743 1107 18-19 0.14769765556472692 1107 20-21 0.13550724631243583 1107 22-23 0.18127604018952326 1107 24-25 0.17036306653423594 1107 26-27 0.14741034298853606 1107 28-29 0.1559618017131683 1107 30-31 0.14453377884890983 1107 32-33 0.20658483154198848 1107 34-35 0.21765868597353233 1107 36-37 0.1973189963148343 1107 38-39 0.18937340950714798 1107 40-41 0.19541365852208514 1107 42-43 0.223760519010888 1107 44-45 0.20412116293670124 1107 46-47 0.2536786351232436 1107 48-49 0.20986487380690022 1107 50-51 0.17580033134840534 1107 52-53 0.17612488233140056 1107 54-55 0.22355516342772574 1107 56-57 0.23812294990556637 1107 58-59 0.15265879746922906 1107 60-61 0.19808303292308693 1107 62-63 0.18317937217894809 1107 64-65 0.17606297524213232 1107 66-67 0.16565051332827352 1107 68-69 0.1346048030602489 1107 70-71 0.11355884809213279 1107 72-73 0.1240473269173279 1107 74-75 0.15506734711865278 1107 76-77 -0.007486158172063995 1107 78-79 0.1329227672788349 1107 80-81 0.1918018220208424 1107 82-83 0.23039272510373365 1107 84-85 0.21109679126551129 1107 86-87 0.1891602269084771 1107 88-89 0.20939502796056786 1107 90-91 0.20015468685479476 1107 92-93 0.1841830649963967 1107 94-95 0.15402656823905403 1107 96-97 0.19790794728533712 1107 98-99 0.22029473425565627 1107 100 0.22884587422778324 1108 1 1.2918532004117402 1108 2 0.9298881502377547 1108 3 1.2294760054065357 1108 4 0.7338445926799437 1108 5 0.3485564708015332 1108 6 0.8784205627470882 1108 7 0.6061908769793263 1108 8 0.26817604332437384 1108 9 0.20506919472436635 1108 10-11 0.13137555603056938 1108 12-13 0.1308039412683044 1108 14-15 0.15240874707298246 1108 16-17 0.15792721625712858 1108 18-19 0.1171870771596204 1108 20-21 0.1258535590091867 1108 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2316 96-97 -0.9447501583564062 2316 98-99 -1.091167293285487 2316 100 -1.040122293937003 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 45.0 7 811.0 8 918.0 9 375.0 10 359.0 11 690.0 12 919.0 13 1371.0 14 3055.0 15 6282.0 16 10176.0 17 14686.0 18 21799.0 19 33733.0 20 49409.0 21 72559.0 22 108427.0 23 177278.0 24 298717.0 25 403470.0 26 415015.0 27 361888.0 28 333943.0 29 358443.0 30 417994.0 31 511663.0 32 629045.0 33 807605.0 34 1111062.0 35 1708422.0 36 3124946.0 37 6387714.0 38 8020221.0 39 2167651.0 40 3267.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 12.753856324420095 25.9550877041765 32.27298501411681 29.018070957286596 2 21.581773395869185 34.125877251669465 26.312463410053823 17.979885942407535 3 24.08639920294858 25.048376006986683 27.844071168405126 23.02115362165961 4 18.319319993487095 29.474886858588402 23.226575671744936 28.97921747617957 5 23.792075030774807 31.474996157117292 26.03768989073878 18.69523892136912 6 30.415149363049952 28.77359151181238 24.032768606007032 16.778490519130635 7 22.42763433542942 25.062713547156594 31.056285526489752 21.45336659092423 8 27.610610768402204 27.021426769121987 24.50232251368049 20.865639948795316 9 25.121167286642944 24.57115193688802 28.41069123672297 21.89698953974607 10-11 21.535207510145092 29.5192416512711 27.9407772651092 21.004773573474605 12-13 21.193346143662144 27.004915894600106 28.206831563053424 23.594906398684326 14-15 21.090837172223235 31.62774192298508 26.13301217481176 21.148408729979927 16-17 23.66575053652957 26.425311334624862 26.237210582815024 23.671727546030546 18-19 20.882706685302598 28.972602555844844 26.51019494370148 23.634495815151073 20-21 23.338205533817955 29.356518351582288 26.39507931090791 20.91019680369185 22-23 23.42159754630197 26.797843095469716 28.731795150084828 21.048764208143492 24-25 21.071940072741015 28.837052937914947 26.228727683107167 23.862279306236868 26-27 21.207114932632688 26.36922025735299 31.367743728680512 21.05592108133381 28-29 21.127735463048655 26.519074426596035 31.354322964414656 20.998867145940658 30-31 20.9046634577952 26.32093111984912 31.53302343219215 21.241381990163536 32-33 21.282434138876773 26.614300062629543 31.049970778865998 21.05329501962768 34-35 21.259990179669668 26.81409522542041 30.825728830078496 21.100185764831426 36-37 21.064651902419698 26.724602204514607 31.092376135282212 21.118369757783483 38-39 21.095806437299373 26.714182141731257 31.1980455009475 20.99196592002187 40-41 21.13079745238173 26.69078640007391 31.14227009040038 21.036146057143974 42-43 21.047367497663622 26.716051013356417 31.143315541591004 21.09326594738896 44-45 21.121955024834715 26.812682521406188 31.070245272514462 20.995117181244634 46-47 21.148997624656193 26.850123978633373 30.990773350567384 21.010105046143046 48-49 21.1459063217234 26.91269172484143 30.92246231231285 21.018939641122326 50-51 21.206627463397194 26.992314466433253 30.871678250968575 20.929379819200978 52-53 21.28194949721011 27.0345515694294 30.67582090765913 21.007678025701356 54-55 21.44272314373533 27.22137918228612 30.149626135624757 21.186271538353797 56-57 21.837529451181194 27.68317410913817 29.208346951633786 21.270949488046856 58-59 21.906207113612876 28.083303713947178 28.635014408801013 21.375474763638934 60-61 21.908968126508952 28.322682541275945 28.42316854657768 21.34518078563742 62-63 21.873268881952235 28.499099934283855 28.226299634818975 21.40133154894494 64-65 21.924838705444884 28.546620515819182 28.115820554386907 21.412720224349027 66-67 21.82716905803587 28.4604897825301 28.304552265192797 21.40778889424123 68-69 21.81718488989533 28.406020447018292 28.386628489161765 21.39016617392461 70-71 21.791230297999366 28.286418418987175 28.454617374239845 21.46773390877361 72-73 21.71980110718874 28.275293281581632 28.50472340929543 21.500182201934198 74-75 21.850466777775235 28.40488369728822 28.27405708834772 21.470592436588827 76-77 21.914293474892187 28.48084961449842 28.078120551725966 21.526736358883426 78-79 21.945947397904405 28.67352432697415 27.78022321864888 21.600305056472564 80-81 22.05926161459805 28.864677498361598 27.4580744149529 21.61798647208745 82-83 22.063389945938084 28.975890308224372 27.117171560509355 21.84354818532819 84-85 22.03011258244084 29.059026081506232 26.971022109413244 21.93983922663968 86-87 22.041525220154707 29.160103793538962 26.762764535265454 22.035606451040877 88-89 22.079510937611236 29.206542400544077 26.55930529831504 22.154641363529652 90-91 22.080058311402244 29.222382863260755 26.453896286133027 22.243662539203974 92-93 22.129012056119038 29.23870927644862 26.36415957008143 22.26811909735091 94-95 22.139640983122142 29.221003162281754 26.21330837712946 22.42604747746664 96-97 22.090104702629024 29.215967942389593 26.153418221783458 22.54050913319793 98-99 22.15670102292016 29.235994081423062 26.03127061696887 22.57603427868791 100 22.0554046112265 29.17952221094826 25.92724819550717 22.83782498231807 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 23154.0 1 17154.0 2 9057.5 3 6496.5 4 5924.0 5 5830.5 6 6041.5 7 6298.0 8 6372.5 9 6634.0 10 7455.0 11 13788.0 12 94975.5 13 111993.0 14 52496.0 15 55592.5 16 63718.0 17 71010.5 18 77552.5 19 84070.5 20 92116.0 21 105965.0 22 127000.5 23 152172.5 24 179043.5 25 207901.0 26 241081.0 27 281978.0 28 337611.5 29 398040.0 30 444353.5 31 486029.0 32 531368.0 33 563631.5 34 589708.0 35 628823.0 36 676970.5 37 721203.0 38 760347.5 39 787137.0 40 800076.0 41 829133.0 42 878457.0 43 900052.5 44 904650.0 45 922518.0 46 939711.0 47 955543.5 48 954557.0 49 945620.0 50 948648.0 51 946584.5 52 933446.5 53 902727.5 54 848623.0 55 787186.5 56 723237.5 57 654285.0 58 580904.0 59 498198.5 60 413277.0 61 337109.0 62 263575.0 63 192692.5 64 135764.0 65 95358.5 66 67064.0 67 47939.0 68 34178.5 69 24523.5 70 17793.5 71 13458.5 72 10437.5 73 8015.5 74 6544.5 75 5818.5 76 4431.5 77 2284.0 78 1009.5 79 592.5 80 381.0 81 265.0 82 203.5 83 146.5 84 117.0 85 92.0 86 62.0 87 44.5 88 26.5 89 17.5 90 18.0 91 13.0 92 8.0 93 5.0 94 3.5 95 4.0 96 4.5 97 3.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33027187169563965 2 0.006319847098881808 3 2.353638762618924 4 8.053995728770157E-4 5 5.441889005925782E-5 6 0.008910186265702481 7 0.00772748238841461 8 2.64838598288388E-4 9 0.0 10-11 5.9860779065183594E-5 12-13 9.069815009876303E-6 14-15 0.0 16-17 2.358151902567839E-5 18-19 0.0 20-21 3.446529703752995E-4 22-23 4.3535112047406254E-5 24-25 5.115375665570235E-4 26-27 0.003622484114944595 28-29 0.006218265170771194 30-31 0.005877240126399844 32-33 0.015532965185914157 34-35 0.010038471252931093 36-37 0.005180678333641344 38-39 0.005438261079921831 40-41 0.00832064829006052 42-43 0.01920261233891011 44-45 0.034409064184468714 46-47 0.0334131984963843 48-49 0.04012486160369276 50-51 0.040465886648064116 52-53 0.0622660940058028 54-55 0.03354561779552849 56-57 0.022003371213959913 58-59 0.016639482617119065 60-61 0.017764139678343727 62-63 0.024682594567877372 64-65 0.0450715387100793 66-67 0.04781425076906589 68-69 0.05034654311982336 70-71 0.061018087460443814 72-73 0.06882357025794336 74-75 0.05959775442989719 76-77 0.059517940057810276 78-79 0.054275586982101776 80-81 0.05170882933430678 82-83 0.04800653084727527 84-85 0.049873098776307814 86-87 0.05804863002621031 88-89 0.06479838635656025 90-91 0.06053920122792235 92-93 0.06495620113773211 94-95 0.0734255943939546 96-97 0.07272540467519215 98-99 0.04744783024266689 100 0.006965617927585001 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.7563958E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.13173936275369 #Duplication Level Percentage of deduplicated Percentage of total 1 67.61387937703142 23.75393187578535 2 15.605086504088087 10.964676635896957 3 5.723732366860203 6.032540210840699 4 2.8565354804967753 4.014202399250843 5 1.7257851524240326 3.0314917085535638 6 1.164001835335916 2.4536045458073006 7 0.8425352782464864 2.0719810859496506 8 0.6100374734563412 1.7145342015184761 9 0.4859160122737121 1.536396722584981 >10 2.9477713738043154 19.366345113164 >50 0.23695951377485752 5.722187925773931 >100 0.17896324622141144 12.489445885899018 >500 0.007930200887861942 1.752569160446387 >1k 7.935376949948505E-4 0.4259206350810187 >5k 2.075640100271755E-5 0.04547344019595462 >10k+ 5.1891002495240066E-5 4.624698453252136 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1147205 4.161974851362057 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 50358 0.18269509770694034 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 32544 0.11806722387256575 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0013604722514814454 1.8139630019752605E-5 0.0 3.6279260039505213E-6 0.0 2 0.001385867733509099 2.539548202765365E-5 0.0 3.6279260039505213E-6 0.0 3 0.0014076352895328022 2.539548202765365E-5 0.0 3.6279260039505213E-6 0.0 4 0.0014584262535881096 3.627926003950521E-5 0.0 3.6279260039505213E-6 1.8139630019752605E-5 5 0.00146205417959206 4.3535112047406254E-5 0.0 3.6279260039505213E-6 2.902340803160417E-5 6 0.001469310031599961 4.3535112047406254E-5 0.0 3.6279260039505213E-6 3.265133403555469E-5 7 0.0014947055136276147 6.167474206715887E-5 0.0 3.6279260039505213E-6 3.627926003950521E-5 8 0.0014983334396315652 6.167474206715887E-5 0.0 3.6279260039505213E-6 4.3535112047406254E-5 9 0.0015273568476631694 6.530266807110938E-5 0.0 3.6279260039505213E-6 4.716303805135677E-5 10-11 0.001616241034759957 6.89305940750599E-5 0.0 3.6279260039505213E-6 5.804681606320834E-5 12-13 0.001725078814878473 7.618644608296094E-5 0.0 3.6279260039505213E-6 5.804681606320834E-5 14-15 0.0017976373349574831 7.981437208691147E-5 0.0 5.441889005925782E-6 5.986077906518361E-5 16-17 0.0018466143360108154 8.344229809086199E-5 0.0 7.255852007901043E-6 7.80004090849362E-5 18-19 0.0019336845601056279 9.069815009876303E-5 0.0 1.0883778011851564E-5 8.525626109283725E-5 20-21 0.0019881034501648856 1.015819281106146E-4 0.0 1.0883778011851564E-5 9.069815009876303E-5 22-23 0.0020769876372616733 1.015819281106146E-4 0.0 1.4511704015802084E-5 1.0702381711654037E-4 24-25 0.00233094245753821 1.0520985411456511E-4 0.0 1.8139630019752605E-5 1.25163447136293E-4 26-27 0.0029531317672157244 1.1246570612246615E-4 0.0 1.8139630019752605E-5 1.4148911415407032E-4 28-29 0.0046727686930882716 1.1790759512839194E-4 0.0 1.9953593021727865E-5 1.4874496616197136E-4 30-31 0.008456695515208664 1.25163447136293E-4 0.0 2.7209445029628908E-5 1.6325667017777346E-4 32-33 0.01643087687189191 1.287913731402435E-4 0.0 2.902340803160417E-5 1.7232648518764976E-4 34-35 0.03103327903779276 1.342332621461693E-4 0.0 2.902340803160417E-5 1.8502422620147657E-4 36-37 0.05534582515326718 1.342332621461693E-4 0.0 2.902340803160417E-5 1.9590800421332815E-4 38-39 0.09210578538829584 1.4148911415407032E-4 0.0 2.902340803160417E-5 2.067917822251797E-4 40-41 0.14247591002714488 1.469310031599961E-4 0.0 2.902340803160417E-5 2.067917822251797E-4 42-43 0.20886876986244138 1.4874496616197136E-4 0.0 2.902340803160417E-5 2.1404763423308075E-4 44-45 0.2943445204785176 1.5237289216592188E-4 0.0 2.902340803160417E-5 2.1767556023703126E-4 46-47 0.40682111037899565 1.560008181698724E-4 0.0 2.902340803160417E-5 2.2855933824888282E-4 48-49 0.5480471998977796 1.5962874417382294E-4 0.0 3.083737103357943E-5 2.394431162607344E-4 50-51 0.7187447463096555 1.5962874417382294E-4 0.0 3.265133403555469E-5 2.5939670928246225E-4 52-53 0.9138564207651165 1.6869855918369924E-4 0.0 3.627926003950521E-5 2.684665242923386E-4 54-55 1.1371389406412535 1.795823371955508E-4 0.0 4.1721149045430995E-5 2.8479219131011594E-4 56-57 1.4006660436792133 1.9228007820937764E-4 0.0 4.3535112047406254E-5 2.9567596932196744E-4 58-59 1.7100936665191553 2.1041970822913023E-4 0.0 4.716303805135677E-5 3.0111785832789324E-4 60-61 2.062943935700381 2.1041970822913023E-4 0.0 5.07909640553073E-5 3.0655974733381905E-4 62-63 2.4516471836156475 2.15861597235056E-4 0.0 5.07909640553073E-5 3.246993773535717E-4 64-65 2.8695806313447436 2.2130348624098178E-4 0.0 5.07909640553073E-5 3.3195522936147273E-4 66-67 3.3356911224433006 2.3400122725480862E-4 0.0 5.07909640553073E-5 3.446529703752995E-4 68-69 3.8494761891597715 2.394431162607344E-4 0.0 5.07909640553073E-5 3.6460656339702734E-4 70-71 4.409881918990008 2.4125707926270966E-4 0.0 5.260492705728256E-5 3.7004845240295314E-4 72-73 5.009305992992734 2.448850052666602E-4 0.0 5.986077906518361E-5 3.8637411942073056E-4 74-75 5.6438302510836795 2.521408572745612E-4 0.0 6.167474206715887E-5 3.918160084266563E-4 76-77 6.316389685400043 2.57582746280487E-4 0.0 6.167474206715887E-5 4.0088582343653263E-4 78-79 7.028803700832805 2.720944502962891E-4 0.0 6.167474206715887E-5 4.063277124424584E-4 80-81 7.7731815583233725 2.775363393022149E-4 0.0 6.167474206715887E-5 4.153975274523347E-4 82-83 8.554883518542585 2.8297822830814065E-4 0.0 6.530266807110938E-5 4.262813054641862E-4 84-85 9.367674990652649 2.902340803160417E-4 0.0 7.437248308098568E-5 4.462348984859141E-4 86-87 10.209001914746786 3.0474578433184376E-4 0.0 7.618644608296094E-5 4.516767874918399E-4 88 10.857395733950835 3.0474578433184376E-4 0.0 7.618644608296094E-5 4.571186764977657E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 158890 0.0 82.025024 8 AGTGGTA 159020 0.0 81.218216 5 GTGGTAT 159815 0.0 80.85239 6 TGGTATC 161225 0.0 80.83973 7 GTATCAA 161245 0.0 80.7365 9 AAGCAGT 158145 0.0 79.488464 1 GCAGTGG 164160 0.0 78.586845 3 CAGTGGT 172605 0.0 74.71218 4 AGCAGTG 174505 0.0 73.70349 2 CAACGCA 155565 0.0 44.299927 12-13 ATCAACG 156560 0.0 44.049866 10-11 ACGCAGA 158000 0.0 43.67983 14-15 AGAGTAC 159180 0.0 43.126564 18-19 GAGTACT 153440 0.0 41.96659 20-21 TCAACGC 155315 0.0 41.954937 12-13 AACGCAG 156605 0.0 41.668022 14-15 AGTACTT 164660 0.0 41.078354 20-21 CGCAGAG 159720 0.0 40.882084 16-17 TATCAAC 160810 0.0 40.512554 10-11 GTACTTT 164205 0.0 39.16447 22-23 >>END_MODULE