FastQCFastQC Report
Thu 26 May 2016
SRR1660714_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660714_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26888427
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17356896.455152620121661No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT458630.17056780599326246No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2258600.083.440681
AGTGGTA2282850.082.156495
GGTATCA2305250.081.1814658
GCAGTGG2327900.081.08363
GTGGTAT2314650.080.974616
TGGTATC2316150.080.624537
GTATCAA2334100.080.110479
AGCAGTG2395900.078.81482
CAGTGGT2395300.078.2673654
GAGTACT2112900.043.33420620-21
AGTACTT2210950.042.7178820-21
AGAGTAC2254450.042.56325518-19
ACGCAGA2308050.042.43803814-15
CAACGCA2310850.042.40018512-13
ATCAACG2315950.042.34853410-11
GTACTTT2211450.041.34721422-23
AACGCAG2300850.040.89525614-15
TCAACGC2304400.040.74405712-13
CGCAGAG2314050.040.57240716-17
TACTTTT2336100.040.46655722-23
Produced by FastQC (version 0.11.5)