##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660714_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26888427 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.9046573457049 34.0 31.0 34.0 30.0 34.0 2 32.03343936779939 34.0 31.0 34.0 30.0 34.0 3 32.090813196324206 34.0 31.0 34.0 30.0 34.0 4 35.49947317483466 37.0 35.0 37.0 33.0 37.0 5 35.47379800238965 37.0 35.0 37.0 33.0 37.0 6 35.548048124942376 37.0 35.0 37.0 33.0 37.0 7 35.48603765478732 37.0 35.0 37.0 33.0 37.0 8 35.50610085893087 37.0 35.0 37.0 33.0 37.0 9 37.18601426554257 39.0 37.0 39.0 34.0 39.0 10-11 37.11565315814123 39.0 37.0 39.0 33.5 39.0 12-13 37.15380023903964 39.0 37.0 39.0 33.5 39.0 14-15 38.53362785037593 40.0 38.0 41.0 34.0 41.0 16-17 38.51140724966916 40.0 38.0 41.0 34.0 41.0 18-19 38.476404328152036 40.0 38.0 41.0 34.0 41.0 20-21 38.342849657958794 40.0 38.0 41.0 33.5 41.0 22-23 38.148578215453064 40.0 38.0 41.0 33.5 41.0 24-25 38.14904553546401 40.0 38.0 41.0 33.0 41.0 26-27 38.151285867336156 40.0 38.0 41.0 33.0 41.0 28-29 38.07573538980172 40.0 38.0 41.0 33.0 41.0 30-31 37.98496866328402 40.0 38.0 41.0 33.0 41.0 32-33 37.856412240106124 40.0 38.0 41.0 32.0 41.0 34-35 37.695663918904586 40.0 38.0 41.0 32.0 41.0 36-37 37.49030895336495 40.0 37.0 41.0 31.0 41.0 38-39 37.01366494960824 39.5 35.0 41.0 31.0 41.0 40-41 36.94563159086993 39.0 35.0 41.0 30.5 41.0 42-43 36.94119310140381 40.0 35.0 41.0 31.0 41.0 44-45 36.93255233933915 40.0 35.0 41.0 31.0 41.0 46-47 37.012405430038726 40.0 35.0 41.0 31.0 41.0 48-49 36.88977360036718 40.0 35.0 41.0 30.0 41.0 50-51 35.50358200574544 38.5 34.0 40.0 28.0 40.5 52-53 35.62113856641744 38.5 34.5 39.5 28.0 40.5 54-55 35.89674544740011 39.0 35.0 41.0 26.0 41.0 56-57 35.41347840838737 39.0 35.0 41.0 22.5 41.0 58-59 35.17412788780839 39.0 35.0 41.0 22.0 41.0 60-61 34.93647015870434 39.0 35.0 41.0 22.0 41.0 62-63 34.701180902103346 38.0 34.0 41.0 21.5 41.0 64-65 34.449342871563296 37.5 34.0 40.0 21.0 41.0 66-67 34.18677617325848 37.0 34.0 40.0 20.5 41.0 68-69 33.91640979965098 36.5 34.0 39.5 20.5 41.0 70-71 33.62214968915809 36.0 33.0 39.0 20.0 41.0 72-73 33.276781196609235 35.5 33.0 39.0 20.0 41.0 74-75 32.8902667493342 35.0 33.0 37.5 20.0 40.0 76-77 32.48753759377594 35.0 33.0 37.0 19.0 39.5 78-79 32.112877410047076 35.0 32.0 37.0 18.0 39.0 80-81 31.790060273886606 35.0 32.0 36.0 17.5 39.0 82-83 31.474054543986526 35.0 32.0 36.0 16.0 37.0 84-85 31.195581746005445 35.0 32.0 35.5 15.0 37.0 86-87 30.989532299527973 35.0 31.0 35.0 15.0 36.5 88-89 30.7954120930912 35.0 31.0 35.0 14.5 36.0 90-91 30.63204359258353 35.0 31.0 35.0 12.0 36.0 92-93 30.48826571372137 35.0 31.0 35.0 11.5 36.0 94-95 30.313325264434397 35.0 31.0 35.0 10.0 35.0 96-97 30.17763052855416 35.0 30.0 35.0 10.0 35.0 98-99 30.07507458506219 34.0 30.0 35.0 10.0 35.0 100 29.9943839779099 34.0 30.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5237325769814056 1101 2 0.5504108947823667 1101 3 0.5378022045943283 1101 4 0.459149875447018 1101 5 0.46804953939371075 1101 6 0.4333319779352536 1101 7 0.45781618448009453 1101 8 0.4701372149416585 1101 9 0.565183009629294 1101 10-11 0.6010771563191852 1101 12-13 0.5478554899058423 1101 14-15 0.6686608718238602 1101 16-17 0.6323222428397557 1101 18-19 0.6393069310772646 1101 20-21 0.6697429890407491 1101 22-23 0.7292054204898051 1101 24-25 0.7304681375743414 1101 26-27 0.6855393939352226 1101 28-29 0.6931400534902252 1101 30-31 0.6681209076409544 1101 32-33 0.6451340788557829 1101 34-35 0.6539538077489553 1101 36-37 0.6032444303280684 1101 38-39 0.5950600183185912 1101 40-41 0.5923893998929017 1101 42-43 0.6214731205824506 1101 44-45 0.6702186536553754 1101 46-47 0.6554803154089583 1101 48-49 0.7298944863910535 1101 50-51 0.6492391387059726 1101 52-53 0.720239072198325 1101 54-55 0.8045311223873881 1101 56-57 0.801187053514461 1101 58-59 0.7072303648300746 1101 60-61 0.7055091087659804 1101 62-63 0.6681972800905953 1101 64-65 0.6422499749066404 1101 66-67 0.6245556800324223 1101 68-69 0.5885458723071508 1101 70-71 0.5448773882970173 1101 72-73 0.5229401244093808 1101 74-75 0.5480245750397685 1101 76-77 0.4950575493248124 1101 78-79 0.5166443747569716 1101 80-81 0.46540708964114685 1101 82-83 0.4780143165903574 1101 84-85 0.4216042660941639 1101 86-87 0.4008388666260707 1101 88-89 0.40646656327282926 1101 90-91 0.3849473697827257 1101 92-93 0.35756677642692125 1101 94-95 0.4205781246383573 1101 96-97 0.3870140093162213 1101 98-99 0.34499591951572484 1101 100 0.3255633590801388 1102 1 0.44876747678404527 1102 2 0.4841580730312174 1102 3 0.4504312634181318 1102 4 0.4214328309788442 1102 5 0.42594910542786835 1102 6 0.3655816106580616 1102 7 0.39601384954229246 1102 8 0.44187885384614134 1102 9 0.48811533670298957 1102 10-11 0.46721662866911373 1102 12-13 0.47192743367345 1102 14-15 0.5650521921498282 1102 16-17 0.535735521529169 1102 18-19 0.49305701844743766 1102 20-21 0.5202920280192913 1102 22-23 0.5587816741519873 1102 24-25 0.5238658896637745 1102 26-27 0.5353745317377872 1102 28-29 0.5711040314178035 1102 30-31 0.5287497421538419 1102 32-33 0.5406776744147308 1102 34-35 0.5235414485656236 1102 36-37 0.5075289137108427 1102 38-39 0.5137847855885767 1102 40-41 0.4790139431707132 1102 42-43 0.5071486388898876 1102 44-45 0.500949414199809 1102 46-47 0.5151059510004714 1102 48-49 0.5646547559711124 1102 50-51 0.5231345729180248 1102 52-53 0.5614722710852575 1102 54-55 0.6157409597088019 1102 56-57 0.6242444649915484 1102 58-59 0.4722989233003858 1102 60-61 0.4788611419516897 1102 62-63 0.4517416239396397 1102 64-65 0.42157091480765274 1102 66-67 0.41968275587836246 1102 68-69 0.420231230661237 1102 70-71 0.4254301857174312 1102 72-73 0.38458615505476246 1102 74-75 0.3673138407496097 1102 76-77 0.34679973402026576 1102 78-79 0.3778162872120774 1102 80-81 0.3531319131397481 1102 82-83 0.33451863507983504 1102 84-85 0.32241847362292475 1102 86-87 0.2646601980335177 1102 88-89 0.23692271161959155 1102 90-91 0.21433120545894369 1102 92-93 0.25589053148408425 1102 94-95 0.27045014632732034 1102 96-97 0.2549520835044454 1102 98-99 0.22390136518058057 1102 100 0.15363304235860653 1103 1 0.38968529146442776 1103 2 0.42416244444598306 1103 3 0.41242632859991346 1103 4 0.36601519396777604 1103 5 0.39442373429336897 1103 6 0.34837024568643216 1103 7 0.32971613652190257 1103 8 0.3743142022501402 1103 9 0.41976950937215207 1103 10-11 0.39038754234303497 1103 12-13 0.4412401879613057 1103 14-15 0.5248820890070789 1103 16-17 0.4780231381764395 1103 18-19 0.46991216230227195 1103 20-21 0.4912564798787855 1103 22-23 0.5160270620443725 1103 24-25 0.544034580238808 1103 26-27 0.5137551685002038 1103 28-29 0.5143452105460398 1103 30-31 0.46061695467589914 1103 32-33 0.43782536018421325 1103 34-35 0.4533394824222654 1103 36-37 0.4418411711167849 1103 38-39 0.44096020649006107 1103 40-41 0.43017964151854926 1103 42-43 0.44550866782699217 1103 44-45 0.44593871321141165 1103 46-47 0.4994584923735985 1103 48-49 0.5287543512594723 1103 50-51 0.4663106774679022 1103 52-53 0.49438095001496407 1103 54-55 0.5917202093851159 1103 56-57 0.6323103947512152 1103 58-59 0.4673585780216527 1103 60-61 0.4806077671301878 1103 62-63 0.4380877797235101 1103 64-65 0.4280551163742885 1103 66-67 0.43659816451371825 1103 68-69 0.42299636778321315 1103 70-71 0.37794640386201195 1103 72-73 0.4162973385367579 1103 74-75 0.445635153488233 1103 76-77 0.4145499267201984 1103 78-79 0.4165307235634117 1103 80-81 0.35892809489949684 1103 82-83 0.38218906498035565 1103 84-85 0.3170557667764271 1103 86-87 0.32731337650906056 1103 88-89 0.32858494327888366 1103 90-91 0.3001731710311617 1103 92-93 0.2787418304613958 1103 94-95 0.36433846245825663 1103 96-97 0.3121899825912351 1103 98-99 0.3027729222318172 1103 100 0.29249680211245277 1104 1 0.3445286777037744 1104 2 0.3791330232233605 1104 3 0.3717679759592585 1104 4 0.3057396977579998 1104 5 0.30900037533722013 1104 6 0.25332389523859433 1104 7 0.2800285061702823 1104 8 0.3039284462798193 1104 9 0.37304822953231564 1104 10-11 0.3265803280580144 1104 12-13 0.34708894531993906 1104 14-15 0.40896533767060106 1104 16-17 0.3955908348737722 1104 18-19 0.36541647036070657 1104 20-21 0.31565845142350923 1104 22-23 0.3809414323838567 1104 24-25 0.42495668440039225 1104 26-27 0.3823258127814242 1104 28-29 0.3501403259810161 1104 30-31 0.32444627472990106 1104 32-33 0.3222172139792221 1104 34-35 0.3316114546037312 1104 36-37 0.30901443453910105 1104 38-39 0.33172374181102526 1104 40-41 0.3057699278038868 1104 42-43 0.323092426107884 1104 44-45 0.313162641756108 1104 46-47 0.36508038053059266 1104 48-49 0.40547949685588236 1104 50-51 0.32957863602961623 1104 52-53 0.3333261810452086 1104 54-55 0.43027815696943605 1104 56-57 0.4158222618601215 1104 58-59 0.3927662998903756 1104 60-61 0.4117846755355643 1104 62-63 0.377907504744158 1104 64-65 0.36881426197903266 1104 66-67 0.373960189285647 1104 68-69 0.349746577300472 1104 70-71 0.3373651878511197 1104 72-73 0.3152705765425807 1104 74-75 0.30650097372620877 1104 76-77 0.34583884437851964 1104 78-79 0.3096985827415253 1104 80-81 0.28409013741499933 1104 82-83 0.2908680676702211 1104 84-85 0.24907266243270243 1104 86-87 0.2377865431586912 1104 88-89 0.2367846614241138 1104 90-91 0.21031167754544455 1104 92-93 0.25130890506765624 1104 94-95 0.3077120921372476 1104 96-97 0.24028429781548866 1104 98-99 0.26167029354399673 1104 100 0.2680554589960522 1105 1 0.3210488939536056 1105 2 0.3496934308325379 1105 3 0.3548856725136815 1105 4 0.30833070952249386 1105 5 0.3428676626965341 1105 6 0.28923422152524836 1105 7 0.3064690923533533 1105 8 0.31453582417516657 1105 9 0.3395982421572157 1105 10-11 0.3493291788599393 1105 12-13 0.3541124728172278 1105 14-15 0.4184926881311739 1105 16-17 0.3676144193901507 1105 18-19 0.4127468414334672 1105 20-21 0.4205062031221374 1105 22-23 0.447047292033254 1105 24-25 0.5169059582595494 1105 26-27 0.47223551299786237 1105 28-29 0.45816846123302923 1105 30-31 0.46839760920804707 1105 32-33 0.4486170530925122 1105 34-35 0.4432956001946238 1105 36-37 0.46056573277800794 1105 38-39 0.47827337931557423 1105 40-41 0.4461972193368311 1105 42-43 0.49893519831767463 1105 44-45 0.5288231006640132 1105 46-47 0.497619142654365 1105 48-49 0.5311408844732384 1105 50-51 0.40756149028877786 1105 52-53 0.45916540990263854 1105 54-55 0.5532927226345805 1105 56-57 0.6173071537824129 1105 58-59 0.5407055115906942 1105 60-61 0.4919335945614307 1105 62-63 0.49538696665765514 1105 64-65 0.48215458304344594 1105 66-67 0.4727072793935818 1105 68-69 0.48986555238022333 1105 70-71 0.4625849707289049 1105 72-73 0.454292372346238 1105 74-75 0.4466096781239415 1105 76-77 0.4249518145877502 1105 78-79 0.46857800930254 1105 80-81 0.42495679541669773 1105 82-83 0.3845474046450583 1105 84-85 0.3517682763830763 1105 86-87 0.3678942401204459 1105 88-89 0.38487612659738346 1105 90-91 0.35339313993745947 1105 92-93 0.3768055714341969 1105 94-95 0.4353937341061105 1105 96-97 0.4004363962729478 1105 98-99 0.35528728998245285 1105 100 0.4256627769340646 1106 1 0.22549473744224358 1106 2 0.27459539496075536 1106 3 0.25311704276565195 1106 4 0.2527740151291056 1106 5 0.2609588164102945 1106 6 0.22049638168535068 1106 7 0.2277529379986163 1106 8 0.2401623583475967 1106 9 0.24703546530332687 1106 10-11 0.24635622043313532 1106 12-13 0.25331348859152314 1106 14-15 0.3276031932782004 1106 16-17 0.30362411110749576 1106 18-19 0.279735991149586 1106 20-21 0.26933071992954183 1106 22-23 0.2651355173507497 1106 24-25 0.2703608118188896 1106 26-27 0.3004908617702924 1106 28-29 0.33836341301931583 1106 30-31 0.3016926582458339 1106 32-33 0.3150203239695841 1106 34-35 0.3271876438178225 1106 36-37 0.2659588198799625 1106 38-39 0.3048802731269973 1106 40-41 0.3385200427193311 1106 42-43 0.31351670058452186 1106 44-45 0.3401681677539443 1106 46-47 0.3121099281977493 1106 48-49 0.30541121284155537 1106 50-51 0.1928859035045818 1106 52-53 0.24852175067643145 1106 54-55 0.3296440055580092 1106 56-57 0.3721738492972264 1106 58-59 0.26892115275910555 1106 60-61 0.25494098757521755 1106 62-63 0.2718119645953081 1106 64-65 0.277048749291815 1106 66-67 0.28272738765316063 1106 68-69 0.29507935235902494 1106 70-71 0.26084004511579195 1106 72-73 0.25498550166413025 1106 74-75 0.2750695955028206 1106 76-77 0.26814243874048316 1106 78-79 0.21580735402055495 1106 80-81 0.16983149245112017 1106 82-83 0.17876364117114107 1106 84-85 0.2123531275659829 1106 86-87 0.2016442376409664 1106 88-89 0.23083570647528973 1106 90-91 0.2057216869114491 1106 92-93 0.20379539059453577 1106 94-95 0.2015318980709857 1106 96-97 0.23282299458060507 1106 98-99 0.23863399080860503 1106 100 0.21130428108538624 1107 1 0.18734365057391855 1107 2 0.22292257918400082 1107 3 0.22752319539270616 1107 4 0.22227309290085628 1107 5 0.23268874167769127 1107 6 0.19324536100783973 1107 7 0.2216949884844439 1107 8 0.22670538236971538 1107 9 0.2647190815667244 1107 10-11 0.23642756650337304 1107 12-13 0.24030612144407826 1107 14-15 0.26919408153202795 1107 16-17 0.25625342843966337 1107 18-19 0.25999973834498036 1107 20-21 0.2486955573362266 1107 22-23 0.29595245754065047 1107 24-25 0.29662200206742995 1107 26-27 0.25762429488879945 1107 28-29 0.26963629451423543 1107 30-31 0.23828118948158306 1107 32-33 0.1979206696741258 1107 34-35 0.24351588817040692 1107 36-37 0.24656519174963165 1107 38-39 0.23391128786794013 1107 40-41 0.25746349302583127 1107 42-43 0.2950593801603887 1107 44-45 0.28344548287047644 1107 46-47 0.28864109868495547 1107 48-49 0.3174950618061487 1107 50-51 0.20450002219650543 1107 52-53 0.20581205749711273 1107 54-55 0.2811899563327742 1107 56-57 0.35981618366457724 1107 58-59 0.29011388611825595 1107 60-61 0.29816552997981915 1107 62-63 0.2514180697393513 1107 64-65 0.25587870440171656 1107 66-67 0.25050917010045737 1107 68-69 0.264259411840726 1107 70-71 0.25274818510049357 1107 72-73 0.2539471414579353 1107 74-75 0.2521579519156276 1107 76-77 0.27232049223540855 1107 78-79 0.287752596251444 1107 80-81 0.2351377904605485 1107 82-83 0.2690358669952495 1107 84-85 0.2745185321553194 1107 86-87 0.24764667363965742 1107 88-89 0.24028006606800645 1107 90-91 0.25548611708990876 1107 92-93 0.24278051174226434 1107 94-95 0.26207401239924266 1107 96-97 0.2970539577905775 1107 98-99 0.2736182460259293 1107 100 0.29264525620904536 1108 1 0.08238898490607127 1108 2 0.11477792763632522 1108 3 0.12549786282610143 1108 4 0.09257889161459332 1108 5 0.13574370813056902 1108 6 0.06891636037609317 1108 7 0.0893622108149259 1108 8 0.08099216751026006 1108 9 0.12513035737046607 1108 10-11 0.04698464996850049 1108 12-13 0.05665468410063568 1108 14-15 0.09290993096244193 1108 16-17 0.06870371036810496 1108 18-19 0.0818024704200937 1108 20-21 0.06174063416082731 1108 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-0.9629054964511852 2316 84-85 -1.06333303925269 2316 86-87 -1.2409539395232407 2316 88-89 -1.1984546855078513 2316 90-91 -1.0859063407457263 2316 92-93 -1.0126612049064576 2316 94-95 -1.1987490726449899 2316 96-97 -1.398768084043386 2316 98-99 -1.2598709474903416 2316 100 -1.0346249743399376 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13814.0 3 3446.0 4 2451.0 5 1950.0 6 2818.0 7 10853.0 8 20204.0 9 24676.0 10 26630.0 11 27321.0 12 30224.0 13 35744.0 14 43810.0 15 54893.0 16 70072.0 17 89692.0 18 101365.0 19 116084.0 20 139195.0 21 175703.0 22 230377.0 23 354733.0 24 505343.0 25 559631.0 26 518466.0 27 433880.0 28 389369.0 29 381507.0 30 418420.0 31 498717.0 32 619042.0 33 796032.0 34 1096549.0 35 1662252.0 36 2920370.0 37 5577828.0 38 6951047.0 39 1980837.0 40 3082.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 15.209985190695008 33.0544620966182 28.588679955460062 23.146872757226724 2 20.80332411213312 34.964221897484514 25.86507832743671 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25.697226841943703 20.204670290920024 22-23 23.985019806044242 26.401448038012955 29.306238976529002 20.307293179413804 24-25 20.34273787857431 29.85285540168519 25.570643960520144 24.233762759220355 26-27 20.450622562220907 26.18815526051253 33.06260552747859 20.298616649787977 28-29 20.374365467352078 26.35421037370394 33.045284297094796 20.226139861849187 30-31 20.278824419202195 26.271119507696657 33.08432472255184 20.365731350549314 32-33 20.422548057963034 26.415118152261247 32.860916515122106 20.301417274653613 34-35 20.4410227951699 26.548034098136252 32.72398127344826 20.28696183324559 36-37 20.313572657344046 26.493971996705557 32.84483407059735 20.34762127535305 38-39 20.3296983322765 26.517657645510017 32.83181519327867 20.32082882893481 40-41 20.339922018371677 26.518786863928256 32.81816640237843 20.32312471532164 42-43 20.348866602016948 26.534163579300422 32.77434961747261 20.342620201210025 44-45 20.390922727226375 26.64975993606135 32.71424460746511 20.245072729247163 46-47 20.36391079174582 26.74080370806179 32.66019840289439 20.235087097298006 48-49 20.40841984360274 26.727884794738877 32.59161324835042 20.272082113307967 50-51 20.428976800830256 26.784264153463607 32.532052057617875 20.254706988088262 52-53 20.57944342269174 26.854484880088968 32.256366148515745 20.309705548703537 54-55 20.957682551367427 27.108976047567673 31.411130204494285 20.522211196570613 56-57 21.510497418392653 27.704566137191765 30.06640918631402 20.718527258101556 58-59 21.681772101284842 28.296001614290944 29.23043630903694 20.791789975387275 60-61 21.882954476919032 28.608249015474673 28.754855614121173 20.75394089348512 62-63 21.94732689795581 28.787659887816584 28.504780422819913 20.760232791407688 64-65 22.025732610380174 28.860350922317075 28.430418519851123 20.683497947451627 66-67 22.003875130160257 28.7036913604869 28.63109504115306 20.661338468199787 68-69 22.078836209211914 28.575587573791474 28.7385094958501 20.60706672114651 70-71 22.19259474962988 28.377275710109622 28.842132623182593 20.587996917077906 72-73 22.361781934427032 28.190966714595355 28.89183905939536 20.555412291582257 74-75 22.671422470949985 28.217950795069957 28.759108110402913 20.351518623577146 76-77 23.04310068005107 28.29107720433904 28.47757245577319 20.188249659836703 78-79 23.377247525322755 28.44431750713087 28.10669689333522 20.071738074211154 80-81 23.77034350792843 28.631767081543654 27.648779046540756 19.949110363987163 82-83 23.98783184440416 28.799896824632103 27.261719332320745 19.950551998642993 84-85 24.149204944381573 28.892369391033547 27.05585821196308 19.902567452621806 86-87 24.34089152154165 28.953893533220043 26.883564535556403 19.821650409681897 88-89 24.61709385380467 28.956955051269347 26.671865570353436 19.75408552457255 90-91 24.817268591348554 28.917667788808384 26.536732566677713 19.728331053165356 92-93 25.015949611369514 28.93427285265531 26.40978177281765 19.639995763157525 94-95 25.190644369664955 28.97782315921593 26.275511325080313 19.556021146038795 96-97 25.321971623791732 28.97005425384687 26.194415427200447 19.513558695160945 98-99 25.539850073142546 28.960726074512117 26.073877228883386 19.425546623461955 100 25.65434736915203 28.939068897101627 25.95972803006481 19.44685570368153 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 21914.0 1 16528.5 2 9732.0 3 7922.5 4 7545.5 5 7704.0 6 7949.5 7 7974.5 8 7915.0 9 8102.0 10 8655.5 11 10746.5 12 94197.0 13 120012.5 14 63733.0 15 68099.5 16 77526.5 17 86557.0 18 95026.0 19 103357.0 20 112948.5 21 128157.5 22 149209.0 23 172704.0 24 197918.0 25 226808.0 26 259979.0 27 299116.5 28 351073.0 29 406201.5 30 449907.0 31 490007.5 32 534235.5 33 573127.5 34 606625.5 35 648810.0 36 696558.5 37 736757.0 38 775878.0 39 801315.5 40 809024.5 41 831330.0 42 868351.5 43 876683.0 44 868561.5 45 880702.5 46 895159.0 47 903502.5 48 900872.0 49 891189.0 50 887252.0 51 881563.5 52 868633.5 53 832123.0 54 774681.5 55 717094.5 56 656539.5 57 591315.0 58 522738.5 59 447290.0 60 367974.5 61 298157.0 62 235592.0 63 174866.0 64 125072.5 65 89169.0 66 63685.0 67 46131.5 68 33407.5 69 24532.0 70 18664.5 71 14362.0 72 11279.5 73 8991.0 74 7230.5 75 6123.5 76 4931.0 77 3348.0 78 2193.0 79 1718.5 80 1436.0 81 1192.5 82 1005.5 83 825.0 84 683.0 85 586.5 86 512.5 87 441.0 88 349.5 89 298.5 90 266.5 91 225.0 92 190.0 93 168.5 94 153.5 95 124.5 96 103.5 97 85.5 98 67.5 99 54.5 100 33.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4015370627668179 2 0.3832466659354971 3 0.16820991425046916 4 0.17439473123511465 5 0.27305055814533147 6 0.10170918514496963 7 0.13595440149771498 8 0.14103093498180463 9 0.13390891181548106 10-11 0.13069191440614952 12-13 0.1326462868207203 14-15 0.1387771772591978 16-17 0.14276402260347917 18-19 0.1543898421428669 20-21 0.13559551103528666 22-23 0.09827648155096615 24-25 0.09356255760145434 26-27 0.0880769261809179 28-29 0.09793804598536018 30-31 0.10230609622496697 32-33 0.10335301503505578 34-35 0.10397038101187547 36-37 0.0998105988126416 38-39 0.09937360783507344 40-41 0.10031081401675151 42-43 0.06304757061467374 44-45 0.06571228580980211 46-47 0.08419793392897248 48-49 0.08884863365194252 50-51 0.09797895577900485 52-53 0.10309267998458965 54-55 0.1053854879647664 56-57 0.10604376373523076 58-59 0.10538734750084117 60-61 0.10326747637561691 62-63 0.10759461682157904 64-65 0.11423687968061501 66-67 0.12299343505665095 68-69 0.12815736673625422 70-71 0.12657676107270982 72-73 0.11331640932360976 74-75 0.11112029721932042 76-77 0.11295008071688241 78-79 0.1116670008252993 80-81 0.10613302146681917 82-83 0.10897997119727384 84-85 0.11481519539986479 86-87 0.1282429053956931 88-89 0.1361626695380879 90-91 0.14292952131413267 92-93 0.14222847621394885 94-95 0.13220929584315214 96-97 0.12891419791868078 98-99 0.12396411288767469 100 0.11819955105592454 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.6888427E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.51574719856276 #Duplication Level Percentage of deduplicated Percentage of total 1 67.39014347862444 23.260211559830736 2 15.536236496969696 10.724896226929804 3 5.956352223401376 6.167638427055572 4 2.9821719183409123 4.1172756814443146 5 1.7742075681255187 3.061904994959861 6 1.1586657858140659 2.399532921046941 7 0.8289397479578717 2.002803234834796 8 0.6354070303869553 1.7545238743220508 9 0.477248525356931 1.4825330506896028 >10 2.830887053349514 17.83277672304518 >50 0.22121409154699267 5.2843259357460255 >100 0.20430043437749484 13.783502261479013 >500 0.0032855704012682615 0.6849448217888068 >1k 8.537736890946193E-4 0.6264435099566161 >5k 5.393853616016951E-5 0.11957139894144739 >10k+ 3.236312169541476E-5 6.69711537792942 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1735689 6.455152620121661 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 45863 0.17056780599326246 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 7.326572134546956E-4 1.4876288598064884E-5 0.0 0.0 3.719072149516221E-6 2 7.810051513984065E-4 1.8595360747581107E-5 0.0 1.1157216448548663E-5 7.438144299032442E-6 3 8.628247386877633E-4 1.8595360747581107E-5 0.0 1.1157216448548663E-5 7.438144299032442E-6 4 9.07453604481958E-4 2.2314432897097326E-5 0.0 1.1157216448548663E-5 7.438144299032442E-6 5 9.223298930800229E-4 2.9752577196129768E-5 0.0 1.1157216448548663E-5 1.4876288598064884E-5 6 9.372061816780877E-4 3.347164934564599E-5 0.0 1.1157216448548663E-5 1.4876288598064884E-5 7 9.40925253827604E-4 3.347164934564599E-5 0.0 1.1157216448548663E-5 1.4876288598064884E-5 8 9.595206145751851E-4 3.7190721495162214E-5 0.0 1.1157216448548663E-5 1.8595360747581107E-5 9 0.001022744841116961 4.0909793644678436E-5 0.0 1.1157216448548663E-5 1.8595360747581107E-5 10-11 0.0011008453562568014 4.648840186895277E-5 0.0 1.3016752523306773E-5 1.8595360747581107E-5 12-13 0.0011808054074714004 5.764561831750143E-5 0.0 1.4876288598064884E-5 1.8595360747581107E-5 14-15 0.001297956180181161 5.9505154392259536E-5 0.0 1.4876288598064884E-5 1.8595360747581107E-5 16-17 0.0013927925199938248 6.322422654177577E-5 0.0 2.0454896822339215E-5 2.2314432897097326E-5 18-19 0.0015378363338249576 7.810051513984064E-5 0.0 2.2314432897097326E-5 2.2314432897097326E-5 20-21 0.0016047796325162496 9.111726766314741E-5 0.0 2.2314432897097326E-5 2.4173968971855438E-5 22-23 0.0017126327248522199 1.0971262841072852E-4 0.0 2.2314432897097326E-5 2.789304112137166E-5 24-25 0.001969248703168839 1.1715077270976096E-4 0.0 2.2314432897097326E-5 2.9752577196129768E-5 26-27 0.002766989679240069 1.1901030878451907E-4 0.0 2.2314432897097326E-5 2.9752577196129768E-5 28-29 0.005143476782780935 1.2644845308355153E-4 0.0 2.2314432897097326E-5 2.9752577196129768E-5 30-31 0.01065886078051349 1.3202706130782584E-4 0.0 2.2314432897097326E-5 2.9752577196129768E-5 32-33 0.022385095267938138 1.3388659738258396E-4 0.0 2.2314432897097326E-5 2.9752577196129768E-5 34-35 0.044591675072699496 1.413247416816164E-4 0.0 2.2314432897097326E-5 2.9752577196129768E-5 36-37 0.08101440816898661 1.413247416816164E-4 0.0 2.2314432897097326E-5 4.2769329719436544E-5 38-39 0.1351808344906156 1.4876288598064885E-4 0.0 2.2314432897097326E-5 4.8347937943710875E-5 40-41 0.20888168727757855 1.6735824672822996E-4 0.0 2.2314432897097326E-5 4.8347937943710875E-5 42-43 0.3043391121392114 1.8967267962532728E-4 0.0 2.2314432897097326E-5 4.8347937943710875E-5 44-45 0.4264343912717542 1.952512878496016E-4 0.0 2.2314432897097326E-5 5.20670100932271E-5 46-47 0.5818376805753642 2.0640850429815027E-4 0.0 2.2314432897097326E-5 5.764561831750143E-5 48-49 0.7744614439513327 2.268634011204895E-4 0.0 2.2314432897097326E-5 5.9505154392259536E-5 50-51 1.0016409662045311 2.3430154541952193E-4 0.0 2.2314432897097326E-5 5.9505154392259536E-5 52-53 1.258997039878904 2.3616108149428005E-4 0.0 2.603350504661355E-5 6.322422654177577E-5 54-55 1.5500609239804173 2.454587618680706E-4 0.0 2.603350504661355E-5 6.322422654177577E-5 56-57 1.8871167138189229 2.5661597831661925E-4 0.0 2.603350504661355E-5 6.322422654177577E-5 58-59 2.2738351336059934 2.640541226156517E-4 0.0 2.603350504661355E-5 6.322422654177577E-5 60-61 2.7068429849020177 2.6777319476516793E-4 0.0 2.603350504661355E-5 6.694329869129198E-5 62-63 3.17491796749583 2.7521133906420036E-4 0.0 2.603350504661355E-5 6.694329869129198E-5 64-65 3.676961095567249 2.882280915875072E-4 0.0 2.9752577196129768E-5 6.880283476605009E-5 66-67 4.221390488926705 2.9194716373702337E-4 0.0 3.7190721495162214E-5 7.25219069155663E-5 68-69 4.809489227465779 2.975257719612977E-4 3.719072149516221E-6 4.0909793644678436E-5 7.438144299032443E-5 70-71 5.441262889792698 2.993853080360558E-4 3.719072149516221E-6 4.462886579419465E-5 7.438144299032443E-5 72-73 6.103932744001723 3.012448441108139E-4 3.719072149516221E-6 4.462886579419465E-5 7.438144299032443E-5 74-75 6.795706197316786 3.0310438018557205E-4 3.719072149516221E-6 4.462886579419465E-5 7.624097906508254E-5 76-77 7.5204975731752555 3.0496391626033014E-4 3.719072149516221E-6 4.8347937943710875E-5 7.810051513984064E-5 78-79 8.274243785253782 3.124020605593626E-4 3.719072149516221E-6 5.20670100932271E-5 7.810051513984064E-5 80-81 9.057207028138908 3.1984020485839506E-4 3.719072149516221E-6 5.20670100932271E-5 8.553865943887309E-5 82-83 9.86393328252337 3.291378852321856E-4 3.719072149516221E-6 5.20670100932271E-5 8.73981955136312E-5 84-85 10.691151624451663 3.4029510168073426E-4 3.719072149516221E-6 5.20670100932271E-5 8.92577315883893E-5 86-87 11.539300904437436 3.5145231812928294E-4 3.719072149516221E-6 5.20670100932271E-5 8.92577315883893E-5 88 12.188407302517176 3.6074999850307346E-4 3.719072149516221E-6 5.578608224274332E-5 8.92577315883893E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 225860 0.0 83.44068 1 AGTGGTA 228285 0.0 82.15649 5 GGTATCA 230525 0.0 81.181465 8 GCAGTGG 232790 0.0 81.0836 3 GTGGTAT 231465 0.0 80.97461 6 TGGTATC 231615 0.0 80.62453 7 GTATCAA 233410 0.0 80.11047 9 AGCAGTG 239590 0.0 78.8148 2 CAGTGGT 239530 0.0 78.267365 4 GAGTACT 211290 0.0 43.334206 20-21 AGTACTT 221095 0.0 42.71788 20-21 AGAGTAC 225445 0.0 42.563255 18-19 ACGCAGA 230805 0.0 42.438038 14-15 CAACGCA 231085 0.0 42.400185 12-13 ATCAACG 231595 0.0 42.348534 10-11 GTACTTT 221145 0.0 41.347214 22-23 AACGCAG 230085 0.0 40.895256 14-15 TCAACGC 230440 0.0 40.744057 12-13 CGCAGAG 231405 0.0 40.572407 16-17 TACTTTT 233610 0.0 40.466557 22-23 >>END_MODULE