FastQCFastQC Report
Thu 26 May 2016
SRR1660714_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660714_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26888427
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16769346.2366385359768355No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT504190.18751189870645835No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT358730.13341427521959542No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2134100.084.7370451
GCAGTGG2269850.081.8592453
AGTGGTA2291100.081.035255
GGTATCA2336150.080.1517948
GTGGTAT2324250.079.784546
TGGTATC2346250.079.716377
AGCAGTG2324750.079.607392
GTATCAA2373400.078.881679
CAGTGGT2392800.077.50324
GAGTACT2144600.043.1748820-21
AGTACTT2250250.042.64769720-21
AGAGTAC2301950.042.2438218-19
CAACGCA2333750.041.9221312-13
ACGCAGA2341000.041.88168314-15
ATCAACG2340250.041.84781310-11
GTACTTT2247800.041.2007222-23
TACTTTT2367300.040.55663722-23
AACGCAG2326350.040.26893614-15
TCAACGC2328800.040.15486512-13
CGCAGAG2344600.039.96464516-17
Produced by FastQC (version 0.11.5)