FastQCFastQC Report
Thu 26 May 2016
SRR1660713_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660713_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34599150
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17176204.964341609548211No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT545460.15765127177979804No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2161400.085.3894048
GTGGTAT2184600.084.599616
TGGTATC2195350.083.873947
AGTGGTA2206500.083.750795
GTATCAA2205950.083.578289
GCAGTGG2273450.081.776153
AAGCAGT2281250.081.461851
CAGTGGT2364550.078.069864
AGCAGTG2431900.076.531472
CAACGCA2132800.045.06636412-13
ATCAACG2140750.044.97448710-11
ACGCAGA2162950.044.46722814-15
AGAGTAC2144000.044.15323618-19
TCAACGC2129400.043.55438212-13
AACGCAG2146150.043.27422714-15
GAGTACT2116050.042.81916820-21
CGCAGAG2174150.042.65253416-17
TATCAAC2192550.042.04476510-11
AGTACTT2261650.041.46022820-21
GTACTTT2242400.040.37690722-23
Produced by FastQC (version 0.11.5)