FastQCFastQC Report
Thu 26 May 2016
SRR1660713_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660713_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34599150
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16064204.642946430764917No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT590790.1707527497062789No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2128200.084.242098
GTGGTAT2128100.083.451156
TGGTATC2160650.082.916497
AGTGGTA2148850.082.691325
GTATCAA2168800.082.6344159
GCAGTGG2155050.082.366483
AAGCAGT2099400.082.290681
CAGTGGT2304750.077.0006264
AGCAGTG2300550.076.843492
CAACGCA2082600.044.8563612-13
ATCAACG2092200.044.7223310-11
ACGCAGA2112400.044.2817214-15
AGAGTAC2126800.043.856718-19
TCAACGC2075450.043.1122612-13
AACGCAG2093300.042.8044214-15
GAGTACT2100500.042.29090520-21
CGCAGAG2128000.042.13106516-17
TATCAAC2152650.041.59011510-11
AGTACTT2244850.041.2673420-21
GTACTTT2219650.040.00884622-23
Produced by FastQC (version 0.11.5)