##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660713_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 34599150 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.265971851909658 34.0 31.0 34.0 27.0 34.0 2 31.91312064024694 34.0 31.0 34.0 30.0 34.0 3 31.683320717416468 34.0 31.0 34.0 30.0 34.0 4 35.60013832709763 37.0 35.0 37.0 33.0 37.0 5 35.93145314263501 37.0 35.0 37.0 35.0 37.0 6 35.37829044933185 37.0 35.0 37.0 33.0 37.0 7 35.5989458700575 37.0 35.0 37.0 35.0 37.0 8 35.96177744250942 37.0 35.0 37.0 35.0 37.0 9 37.76866480823951 39.0 38.0 39.0 35.0 39.0 10-11 37.803220483740205 39.0 37.5 39.0 35.0 39.0 12-13 37.864412666207116 39.0 38.0 39.0 35.0 39.0 14-15 39.31978395423009 41.0 39.0 41.0 36.0 41.0 16-17 39.285864016890585 41.0 39.0 41.0 36.0 41.0 18-19 39.289489698446346 41.0 39.0 41.0 36.0 41.0 20-21 39.23840212837598 40.5 39.0 41.0 36.0 41.0 22-23 39.1311475137395 40.0 39.0 41.0 35.5 41.0 24-25 39.13719272005237 40.0 39.0 41.0 36.0 41.0 26-27 39.08415384481989 40.0 39.0 41.0 36.0 41.0 28-29 39.006969809951975 40.0 39.0 41.0 35.0 41.0 30-31 38.91886595479947 40.0 38.5 41.0 35.0 41.0 32-33 38.806529278898466 40.0 38.0 41.0 35.0 41.0 34-35 38.72933327841869 40.0 38.0 41.0 35.0 41.0 36-37 38.61711059375736 40.0 38.0 41.0 35.0 41.0 38-39 38.26521248354367 40.0 37.5 41.0 34.0 41.0 40-41 38.14488110257044 40.0 37.0 41.0 34.0 41.0 42-43 37.99262934205031 40.0 37.0 41.0 33.0 41.0 44-45 37.87916895357256 40.0 37.0 41.0 33.0 41.0 46-47 37.73966669701423 40.0 36.0 41.0 33.0 41.0 48-49 37.79718235852614 40.0 36.5 41.0 33.0 41.0 50-51 37.814812430363176 40.0 37.0 41.0 33.0 41.0 52-53 37.59750488090025 40.0 36.0 41.0 33.0 41.0 54-55 37.134686459060404 40.0 36.0 41.0 31.5 41.0 56-57 36.6931350192129 40.0 36.0 41.0 30.5 41.0 58-59 36.56443289791801 39.5 36.0 41.0 30.0 41.0 60-61 36.422977948880245 39.0 35.0 41.0 30.0 41.0 62-63 36.198525888641775 39.0 35.0 41.0 29.5 41.0 64-65 35.94760307695421 38.5 35.0 41.0 29.0 41.0 66-67 35.68130224297418 38.0 35.0 40.0 29.0 41.0 68-69 35.429112261428386 37.0 35.0 40.0 29.0 41.0 70-71 35.1399144776678 37.0 35.0 39.5 29.0 41.0 72-73 34.79726240384518 36.0 34.0 39.0 29.0 41.0 74-75 34.41561555702958 36.0 34.0 39.0 28.0 40.5 76-77 32.70226082143637 34.5 32.0 36.5 25.5 39.0 78-79 33.6374880163241 35.0 34.0 37.0 27.0 39.0 80-81 33.53121735649575 35.0 34.0 37.0 27.0 39.0 82-83 33.265415768884495 35.0 34.0 36.0 27.0 38.5 84-85 33.0118442216066 35.0 34.0 36.0 27.0 37.0 86-87 32.79376291036051 35.0 34.0 35.5 26.5 37.0 88-89 32.61859071682397 35.0 34.0 35.0 26.0 36.5 90-91 32.46085097466268 35.0 34.0 35.0 26.0 36.0 92-93 32.32048271417072 35.0 33.5 35.0 25.5 36.0 94-95 32.21429825588201 35.0 33.0 35.0 25.0 36.0 96-97 32.11329669948539 35.0 33.0 35.0 25.0 35.5 98-99 32.027024189900615 35.0 33.0 35.0 25.0 35.0 100 31.97614262200083 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.6025425427004336 1101 2 1.2166753714531389 1101 3 1.546474920399028 1101 4 0.963514175450662 1101 5 0.5776548868743632 1101 6 1.1268829812364558 1101 7 0.8270059375271259 1101 8 0.4889989572624174 1101 9 0.5106683075999925 1101 10-11 0.4194397833873964 1101 12-13 0.3930517652077725 1101 14-15 0.512223462211864 1101 16-17 0.5432379215887693 1101 18-19 0.5135766304369653 1101 20-21 0.5164936289859909 1101 22-23 0.49241027607236987 1101 24-25 0.49241501832378987 1101 26-27 0.5181272747095491 1101 28-29 0.49205749323463266 1101 30-31 0.49576952670834373 1101 32-33 0.5223344849401741 1101 34-35 0.5439786800453277 1101 36-37 0.5229547264663452 1101 38-39 0.5759096506505941 1101 40-41 0.5608218707646344 1101 42-43 0.5550147244664103 1101 44-45 0.5407503703912937 1101 46-47 0.5587157460693817 1101 48-49 0.514617163034238 1101 50-51 0.494395387323884 1101 52-53 0.49977182965330513 1101 54-55 0.5057280744483563 1101 56-57 0.5378892101402428 1101 58-59 0.5165195778578209 1101 60-61 0.5215144761519284 1101 62-63 0.49861293631038706 1101 64-65 0.4608221190218629 1101 66-67 0.46062408601127913 1101 68-69 0.44160967540336316 1101 70-71 0.41153645843723297 1101 72-73 0.4118232917839393 1101 74-75 0.4042846488711689 1101 76-77 0.3423709167877078 1101 78-79 0.4415023366485613 1101 80-81 0.4523015702955462 1101 82-83 0.4219057778949562 1101 84-85 0.4007480998825912 1101 86-87 0.38935076496536425 1101 88-89 0.40194573228323094 1101 90-91 0.40914100643158235 1101 92-93 0.37636730487563597 1101 94-95 0.33492381819495876 1101 96-97 0.3128459243449768 1101 98-99 0.36099305878286003 1101 100 0.36405606113256894 1102 1 1.5121662107243026 1102 2 1.139842155287159 1102 3 1.478349768216674 1102 4 0.8965821329455395 1102 5 0.48329023998172715 1102 6 1.050906296062891 1102 7 0.7652628542909241 1102 8 0.42849737350421435 1102 9 0.4360231498265179 1102 10-11 0.3418310928777828 1102 12-13 0.332406498412098 1102 14-15 0.4477342586117743 1102 16-17 0.45449733242627843 1102 18-19 0.4010690115731492 1102 20-21 0.3825556205509386 1102 22-23 0.4136293961384325 1102 24-25 0.4116174698038506 1102 26-27 0.406542826080063 1102 28-29 0.428949034621148 1102 30-31 0.41244037709381587 1102 32-33 0.4222276159739522 1102 34-35 0.3760235324969159 1102 36-37 0.39758879456488216 1102 38-39 0.4123296361658433 1102 40-41 0.4391080201438342 1102 42-43 0.43858306601074304 1102 44-45 0.41431692000341513 1102 46-47 0.4181016886215829 1102 48-49 0.4002802537659633 1102 50-51 0.37090092806390373 1102 52-53 0.3244748445087495 1102 54-55 0.3876777912577083 1102 56-57 0.38472828616678356 1102 58-59 0.38225428217690194 1102 60-61 0.36002656326723326 1102 62-63 0.321472318004524 1102 64-65 0.289804116058491 1102 66-67 0.2895409434419207 1102 68-69 0.24841140684583962 1102 70-71 0.24469339709506954 1102 72-73 0.2333387243149403 1102 74-75 0.24969848518239246 1102 76-77 0.09084421774416995 1102 78-79 0.282737883699987 1102 80-81 0.2985890324533784 1102 82-83 0.29996058686738536 1102 84-85 0.28546751982607077 1102 86-87 0.2571626110208385 1102 88-89 0.3064817560430342 1102 90-91 0.3006478797573706 1102 92-93 0.299749228403563 1102 94-95 0.21337906811343288 1102 96-97 0.24691865615643138 1102 98-99 0.266455648523376 1102 100 0.2840005055936068 1103 1 1.500222351217758 1103 2 1.1198052278267205 1103 3 1.4423968236640974 1103 4 0.8950204594706932 1103 5 0.5045089438423389 1103 6 1.044985304920857 1103 7 0.7657221695329852 1103 8 0.43226570231130523 1103 9 0.44691300920180055 1103 10-11 0.3605526706849389 1103 12-13 0.32709787218678343 1103 14-15 0.38710713115600015 1103 16-17 0.41446402490630163 1103 18-19 0.3742209957280451 1103 20-21 0.37132010502317314 1103 22-23 0.37106040513507565 1103 24-25 0.3949760373113449 1103 26-27 0.3897585714861762 1103 28-29 0.3811238468042859 1103 30-31 0.3819093559962923 1103 32-33 0.41688349857749785 1103 34-35 0.3980764956855296 1103 36-37 0.3802039078818851 1103 38-39 0.39531016338869307 1103 40-41 0.4220903143370336 1103 42-43 0.4270870810907965 1103 44-45 0.43051941600207044 1103 46-47 0.42927633788003305 1103 48-49 0.4155490780938962 1103 50-51 0.4217172053843612 1103 52-53 0.3853700873878907 1103 54-55 0.40899919878529545 1103 56-57 0.4391099565507943 1103 58-59 0.44133733772216033 1103 60-61 0.4215215859807202 1103 62-63 0.3861822634995562 1103 64-65 0.3809038890324814 1103 66-67 0.3854129750001505 1103 68-69 0.3605641085156108 1103 70-71 0.3796832220198425 1103 72-73 0.3559466199478294 1103 74-75 0.4147732401070172 1103 76-77 0.258165576603929 1103 78-79 0.40462324126018956 1103 80-81 0.40733979175248436 1103 82-83 0.410115693206734 1103 84-85 0.36805980190403886 1103 86-87 0.33836482739115326 1103 88-89 0.35087198139230935 1103 90-91 0.37392055474882824 1103 92-93 0.37180097799378586 1103 94-95 0.34881089372602503 1103 96-97 0.32974315468451465 1103 98-99 0.3677669077667929 1103 100 0.35409130371072095 1104 1 1.4250225135547367 1104 2 1.044933926697741 1104 3 1.3890418318318751 1104 4 0.8593344662771685 1104 5 0.4740213180851356 1104 6 0.9955936068636788 1104 7 0.7206737620960766 1104 8 0.39679482458818427 1104 9 0.3878274801186592 1104 10-11 0.3047969320354085 1104 12-13 0.27343289227184187 1104 14-15 0.3145006657834273 1104 16-17 0.33393976697631444 1104 18-19 0.29036929356892216 1104 20-21 0.30775868183965116 1104 22-23 0.2937341126696893 1104 24-25 0.2965442987830684 1104 26-27 0.3024346773297353 1104 28-29 0.30399987457924027 1104 30-31 0.3223152976430512 1104 32-33 0.3219218983294212 1104 34-35 0.3419447424811821 1104 36-37 0.3387921731628154 1104 38-39 0.3656568711284649 1104 40-41 0.3382559048253668 1104 42-43 0.3693799143957506 1104 44-45 0.3390969247819058 1104 46-47 0.3523393577151168 1104 48-49 0.28391992427796 1104 50-51 0.2895432441661967 1104 52-53 0.29920666030671583 1104 54-55 0.2896861996826132 1104 56-57 0.3383394877388497 1104 58-59 0.37865567330935335 1104 60-61 0.351893606574734 1104 62-63 0.3308783993694604 1104 64-65 0.2977536277982509 1104 66-67 0.32754155507474536 1104 68-69 0.28032184088695544 1104 70-71 0.2975585000037242 1104 72-73 0.2977768997923178 1104 74-75 0.2900273913607734 1104 76-77 0.16939585200785245 1104 78-79 0.32565753344591997 1104 80-81 0.3465321611958885 1104 82-83 0.3646882823144608 1104 84-85 0.33796235232783545 1104 86-87 0.34785852382849924 1104 88-89 0.2956250310315198 1104 90-91 0.29150474573534524 1104 92-93 0.3324304571749721 1104 94-95 0.31641669331880706 1104 96-97 0.28170669602318554 1104 98-99 0.3153713336100523 1104 100 0.3471950156952026 1105 1 1.3994486441714642 1105 2 1.028957178215908 1105 3 1.3521572464217542 1105 4 0.8393847004366393 1105 5 0.43312022158488617 1105 6 0.9811008364466645 1105 7 0.7295311882272486 1105 8 0.3616408689145274 1105 9 0.34708812065309047 1105 10-11 0.25370452447981506 1105 12-13 0.25590026058186055 1105 14-15 0.3280380712005879 1105 16-17 0.33470902964921834 1105 18-19 0.29024327442913744 1105 20-21 0.32321278142494947 1105 22-23 0.30343583822785547 1105 24-25 0.312781034877446 1105 26-27 0.29620940907616244 1105 28-29 0.30634369643900783 1105 30-31 0.30386734119233694 1105 32-33 0.3427988556306474 1105 34-35 0.35540690568794986 1105 36-37 0.32769728351235017 1105 38-39 0.37513536292537 1105 40-41 0.366924581804426 1105 42-43 0.3409345296188562 1105 44-45 0.3056155843743369 1105 46-47 0.362082592296737 1105 48-49 0.33997287084426375 1105 50-51 0.3495766921797596 1105 52-53 0.33191635018246046 1105 54-55 0.35919593197916555 1105 56-57 0.344717380811403 1105 58-59 0.3295738406722961 1105 60-61 0.3027142853744422 1105 62-63 0.28726740169306453 1105 64-65 0.2601250485586988 1105 66-67 0.3113108927255155 1105 68-69 0.2822997500782449 1105 70-71 0.2654315600178805 1105 72-73 0.24866351819721189 1105 74-75 0.255632462983165 1105 76-77 0.1602606772508679 1105 78-79 0.26215926334697315 1105 80-81 0.281704001156605 1105 82-83 0.31552394390155314 1105 84-85 0.30953123306750285 1105 86-87 0.3192466281253914 1105 88-89 0.27587606860187464 1105 90-91 0.25649314343480967 1105 92-93 0.2520367000627104 1105 94-95 0.2609652840241168 1105 96-97 0.2692862620616481 1105 98-99 0.27160727737356893 1105 100 0.28712235063746405 1106 1 1.3361273017200688 1106 2 0.9862865054413597 1106 3 1.3216422586035392 1106 4 0.783555187339644 1106 5 0.39708513133480494 1106 6 0.9451096904001588 1106 7 0.669720629209948 1106 8 0.3284129384714092 1106 9 0.2923036459708541 1106 10-11 0.2159154004704007 1106 12-13 0.1946513977308939 1106 14-15 0.2466192915985701 1106 16-17 0.23338147505160123 1106 18-19 0.20903840776471583 1106 20-21 0.22591484741403178 1106 22-23 0.24323117133890548 1106 24-25 0.2437922044429186 1106 26-27 0.21911517988007034 1106 28-29 0.23383871517766153 1106 30-31 0.26154629711971467 1106 32-33 0.2720554058715976 1106 34-35 0.2649596529735021 1106 36-37 0.2606935026899393 1106 38-39 0.2551547671031713 1106 40-41 0.2453767172504513 1106 42-43 0.26840181271990815 1106 44-45 0.2354245163507187 1106 46-47 0.2654367257941672 1106 48-49 0.2503350815866199 1106 50-51 0.26469590663876374 1106 52-53 0.2340077724280576 1106 54-55 0.2478518200712685 1106 56-57 0.24183262323544596 1106 58-59 0.23571848948033391 1106 60-61 0.24064044850825894 1106 62-63 0.20562469270166872 1106 64-65 0.1824314656968511 1106 66-67 0.1986391189672858 1106 68-69 0.25089120056849623 1106 70-71 0.251316754224824 1106 72-73 0.16846462876962676 1106 74-75 0.18915816976706878 1106 76-77 0.05770078078501939 1106 78-79 0.1919999775884378 1106 80-81 0.21153996989807666 1106 82-83 0.20844447959804313 1106 84-85 0.22895664950188888 1106 86-87 0.21863736136833012 1106 88-89 0.21419833805401822 1106 90-91 0.19955692377648404 1106 92-93 0.19341073686091903 1106 94-95 0.17725267641688447 1106 96-97 0.2094784284741209 1106 98-99 0.22084061120828125 1106 100 0.23194446090150578 1107 1 1.2878497221676852 1107 2 0.9311820662127737 1107 3 1.2790450443911396 1107 4 0.7699824364595358 1107 5 0.38269044126416674 1107 6 0.9404474343329312 1107 7 0.6567969584699327 1107 8 0.291808928111287 1107 9 0.25486786628049884 1107 10-11 0.20399602807652428 1107 12-13 0.1611249671405659 1107 14-15 0.19564617780808646 1107 16-17 0.20040433764143017 1107 18-19 0.164750836937543 1107 20-21 0.15246780386831205 1107 22-23 0.17448527170730443 1107 24-25 0.16568402776228908 1107 26-27 0.1604038620899857 1107 28-29 0.1480665763769835 1107 30-31 0.13653186697089836 1107 32-33 0.17295045374162754 1107 34-35 0.195147763799838 1107 36-37 0.17610820841058938 1107 38-39 0.15877091384928121 1107 40-41 0.17389268855338713 1107 42-43 0.2173463606100725 1107 44-45 0.18351340692959894 1107 46-47 0.2278735980007056 1107 48-49 0.2088205668499512 1107 50-51 0.1788969995927019 1107 52-53 0.17563316890781522 1107 54-55 0.20912277765022935 1107 56-57 0.23912358365826236 1107 58-59 0.17250661502426112 1107 60-61 0.1668643735706823 1107 62-63 0.14463434262540176 1107 64-65 0.11279815902435786 1107 66-67 0.13093772444450735 1107 68-69 0.11480239440674467 1107 70-71 0.1229663125979954 1107 72-73 0.10708270657224261 1107 74-75 0.12135347199048141 1107 76-77 0.01309290288629228 1107 78-79 0.16655691578144172 1107 80-81 0.20009743262107094 1107 82-83 0.20632906865496636 1107 84-85 0.22069405403887998 1107 86-87 0.19010447469542413 1107 88-89 0.21566303375998785 1107 90-91 0.18520134198320903 1107 92-93 0.19310477773512957 1107 94-95 0.14858976632388732 1107 96-97 0.19585549197237384 1107 98-99 0.20596901919896737 1107 100 0.2431940815848712 1108 1 1.266917298815308 1108 2 0.9066206122618503 1108 3 1.2334431268832944 1108 4 0.7263122927582231 1108 5 0.3448464185000404 1108 6 0.8861677430035684 1108 7 0.6064343911942913 1108 8 0.24474633207560004 1108 9 0.20318268293148378 1108 10-11 0.13746800050979147 1108 12-13 0.11866769688555223 1108 14-15 0.13027831966691394 1108 16-17 0.1261492366303969 1108 18-19 0.10567237533275176 1108 20-21 0.1263028811300373 1108 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20.30941552097094 24-25 20.337633030819156 29.53655330548791 26.782063502470717 23.343750161222218 26-27 20.38740921286582 26.784642587638974 32.46171797854926 20.36623022094595 28-29 20.346195995701887 26.932614586107984 32.43227287344258 20.28891654474755 30-31 20.269485621663772 26.795046722101162 32.489982092610724 20.445485563624345 32-33 20.52990077567289 26.98380650772023 32.144494821114804 20.34179789549207 34-35 20.512228658435152 27.150753903493495 31.99936084618046 20.337656591890894 36-37 20.392405855770523 27.06336639112183 32.1318955410341 20.412332212073544 38-39 20.413124279333484 27.060795154814482 32.18215819245847 20.343922373393564 40-41 20.445083953981268 27.048637521386137 32.167344636477 20.338933888155598 42-43 20.40248374895731 27.0753320798003 32.118396676239655 20.403787495002728 44-45 20.41745207110268 27.14117758218369 32.083831927093655 20.357538419619978 46-47 20.448345609592536 27.201058438397723 32.0264207142577 20.324175237752037 48-49 20.492608333453447 27.208537693602498 31.939496026132865 20.359357946811194 50-51 20.46614306178913 27.285308487652145 31.89509549658246 20.353452953976266 52-53 20.552731728818507 27.401603400418693 31.65327058095682 20.392394289805978 54-55 20.821657168670587 27.561874571461164 31.053242298942706 20.56322596092554 56-57 21.237405935690433 28.036628021906196 30.001638553107885 20.72432748929549 58-59 21.29472360775502 28.524302517784395 29.36619598812964 20.814777886330944 60-61 21.302104856671882 28.82243688434203 29.075591996946322 20.799866262039764 62-63 21.220029483479706 28.9784025597159 28.93550935107074 20.86605860573366 64-65 21.296558557783047 29.090958431375768 28.819199841866567 20.793283168974618 66-67 21.20043085375551 28.976457745969782 29.0154977228367 20.80761367743801 68-69 21.189598828737573 28.864308622907803 29.169905634488273 20.776186913866347 70-71 21.139334230035868 28.750893022427483 29.317840617906306 20.791932129630343 72-73 21.13911530317807 28.705289792896497 29.31798038307833 20.837614520847104 74-75 21.204187346077482 28.808861309850226 29.12792437239638 20.859026971675913 76-77 21.265979255567636 28.94843141394119 28.919757432869233 20.865831897621945 78-79 21.34412788817916 29.111654806090705 28.603088689437456 20.94112861629268 80-81 21.45782227016014 29.294721001021923 28.281352518827337 20.9661042099906 82-83 21.487469991650972 29.4404845531661 27.977638894909123 21.09440656027381 84-85 21.50357271646211 29.498965776432385 27.82932593659726 21.168135570508245 86-87 21.527714827776244 29.580573038525227 27.660065914067978 21.23164621963055 88-89 21.574457524536875 29.655824209372845 27.435684671783978 21.334033594306305 90-91 21.65664559593209 29.659031507773374 27.262873259639985 21.421449636654547 92-93 21.66588272681039 29.716714884258046 27.182743719314256 21.43465866961731 94-95 21.705288665674 29.75825457372628 27.034935077556376 21.501521683043343 96-97 21.717488524840235 29.772473439119334 26.919175304957832 21.590862731082602 98-99 21.758638939767753 29.80343748303884 26.785713614445285 21.652209962748124 100 21.67198181469016 29.82839469503406 26.70233209417911 21.79729139609667 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 22229.0 1 17948.5 2 11194.5 3 8009.5 4 7250.0 5 7243.0 6 7507.0 7 7485.5 8 7301.5 9 7546.0 10 8557.0 11 16627.5 12 124926.5 13 148462.0 14 69989.5 15 74655.5 16 86004.5 17 97135.0 18 107928.5 19 119554.0 20 135565.5 21 162026.5 22 201010.0 23 244523.5 24 284899.5 25 328050.0 26 372899.0 27 425195.5 28 508773.0 29 598046.0 30 652604.0 31 707844.5 32 775687.5 33 813680.5 34 847099.5 35 918849.0 36 990909.0 37 1018416.5 38 1044042.5 39 1072473.0 40 1084601.5 41 1125121.0 42 1200429.0 43 1215206.0 44 1177004.0 45 1160452.5 46 1152476.0 47 1148294.5 48 1122868.0 49 1088225.0 50 1069675.0 51 1050684.5 52 1024260.0 53 983363.0 54 919263.5 55 836920.5 56 751025.5 57 671771.5 58 593836.0 59 503223.0 60 413108.0 61 338576.5 62 264295.5 63 189608.0 64 131321.0 65 90771.0 66 62865.5 67 46259.5 68 33826.5 69 23678.5 70 17067.0 71 13100.0 72 10639.0 73 8721.0 74 7960.0 75 8117.0 76 6187.5 77 2666.0 78 872.5 79 523.0 80 344.0 81 239.5 82 174.5 83 125.0 84 109.0 85 87.5 86 55.5 87 38.0 88 27.5 89 19.0 90 11.0 91 3.5 92 4.5 93 7.0 94 3.5 95 1.0 96 0.5 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3340399980924387 2 0.006381659665049575 3 2.3662604428143466 4 9.30658701153063E-4 5 3.468293296222595E-5 6 0.008783452772683721 7 0.007873025782425292 8 3.2370737431410885E-4 9 5.7804888270376584E-6 10-11 8.526221019880546E-5 12-13 1.4451222067594146E-6 14-15 0.0 16-17 1.8786588687872388E-5 18-19 0.0 20-21 3.396037185884624E-4 22-23 3.7573177375744776E-5 24-25 4.7833545043736626E-4 26-27 0.0034278298744333316 28-29 0.0060709583905963005 30-31 0.005721238816560522 32-33 0.015361649057852577 34-35 0.009852843205685689 36-37 0.0050752691901390645 38-39 0.005309378987634089 40-41 0.008224190478667829 42-43 0.01885450943159008 44-45 0.03431876216612258 46-47 0.033502268119303506 48-49 0.04012959855950218 50-51 0.040573251076977325 52-53 0.06289460868258324 54-55 0.03299358510252419 56-57 0.022127711229900156 58-59 0.016644917577454937 60-61 0.017715753132663662 62-63 0.02483442512316054 64-65 0.04537250192562534 66-67 0.04812256948508851 68-69 0.05104171634274252 70-71 0.06170960847304052 72-73 0.0692199085815692 74-75 0.06033674237661908 76-77 0.060011589880098214 78-79 0.054020113210873674 80-81 0.051589417659104346 82-83 0.04824106950604278 84-85 0.05032060036156957 86-87 0.05878901649317975 88-89 0.06507963345920348 90-91 0.061200925456261206 92-93 0.06569670064148975 94-95 0.07396135454194683 96-97 0.07292231167528682 98-99 0.04758209377976049 100 0.0070348549025048305 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.459915E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.094516113149965 #Duplication Level Percentage of deduplicated Percentage of total 1 72.76865920475285 24.810122237887644 2 12.770666665367077 8.7081940079605 3 4.576623617401736 4.681133030019785 4 2.4083015799624974 3.2843950809342353 5 1.4641220444106011 2.495926631738765 6 1.0026805315220373 2.0511544522991922 7 0.715516525209515 1.7076632788586616 8 0.5623287664591475 1.533786175114332 9 0.4317782482970359 1.324914339948386 >10 2.8473925297128115 18.65363414586101 >50 0.24871047705642488 5.83844914524524 >100 0.1753742558133266 13.219039021480103 >500 0.026464017059622324 5.893715289354051 >1k 0.0012873817460583043 0.6206748304235647 >5k 5.135739793693339E-5 0.12234276336523503 >10k+ 4.279783146471017E-5 5.0548555695091775 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1606420 4.642946430764917 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 59079 0.1707527497062789 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.2254881984094986E-4 0.0 0.0 0.0 0.0 2 2.3121955308150633E-4 8.670733240556488E-6 0.0 0.0 2.8902444135188292E-6 3 2.3121955308150633E-4 1.1560977654075317E-5 0.0 0.0 5.7804888270376584E-6 4 2.3121955308150633E-4 2.0231710894631803E-5 0.0 2.8902444135188292E-6 1.1560977654075317E-5 5 2.3121955308150633E-4 2.3121955308150634E-5 0.0 2.8902444135188292E-6 1.7341466481112976E-5 6 2.3410979749502515E-4 2.890244413518829E-5 0.0 2.8902444135188292E-6 1.7341466481112976E-5 7 2.3410979749502515E-4 3.468293296222595E-5 0.0 5.7804888270376584E-6 2.0231710894631803E-5 8 2.3410979749502515E-4 3.468293296222595E-5 0.0 5.7804888270376584E-6 2.3121955308150634E-5 9 2.37000041908544E-4 3.468293296222595E-5 0.0 5.7804888270376584E-6 2.890244413518829E-5 10-11 2.37000041908544E-4 4.1908543996023025E-5 0.0 5.7804888270376584E-6 3.0347566341947707E-5 12-13 2.3989028632206283E-4 5.346952165009834E-5 0.0 5.7804888270376584E-6 3.323781075546654E-5 14-15 2.3989028632206283E-4 6.069513268389541E-5 0.0 5.7804888270376584E-6 4.1908543996023025E-5 16-17 2.442256529423411E-4 6.503049930417366E-5 0.0 5.7804888270376584E-6 5.057927723657951E-5 18-19 2.514512639761381E-4 7.370123254473015E-5 0.0 1.0115855447315903E-5 5.491464385685775E-5 20-21 2.54341508389657E-4 7.94817213717678E-5 0.0 1.300609986083473E-5 6.069513268389541E-5 22-23 2.7312809707752937E-4 9.248782123260254E-5 0.0 1.4451222067594146E-5 6.069513268389541E-5 24-25 3.641707961033725E-4 1.0404879888667786E-4 0.0 1.4451222067594146E-5 7.514635475148955E-5 26-27 6.459696264214583E-4 1.1271953212723434E-4 0.0 1.589634427435356E-5 9.248782123260254E-5 28-29 0.0016228722381908226 1.300609986083473E-4 0.0 1.7341466481112976E-5 9.537806564612136E-5 30-31 0.004299238565109258 1.387317318489038E-4 0.0 1.7341466481112976E-5 9.82683100596402E-5 32-33 0.01017510545779304 1.4162197626242263E-4 0.0 1.7341466481112976E-5 1.0838416550695609E-4 34-35 0.021435497692862397 1.4740246508946028E-4 0.0 1.8786588687872388E-5 1.18500020954272E-4 36-37 0.04047353764471093 1.5462807612325735E-4 0.0 2.0231710894631803E-5 1.286158764015879E-4 38-39 0.06844965844536643 1.661890537773327E-4 0.0 2.0231710894631803E-5 1.3295124302186613E-4 40-41 0.10826277524158831 1.7196954260437035E-4 0.0 2.0231710894631803E-5 1.387317318489038E-4 42-43 0.1611889309419451 1.8497564246520507E-4 0.0 2.167683310139122E-5 1.5029270950297913E-4 44-45 0.23024698583635725 1.9075613129224272E-4 0.0 2.3121955308150634E-5 1.6474393157057325E-4 46-47 0.31767976958971533 1.979817423260398E-4 0.0 2.3121955308150634E-5 1.7485978701788917E-4 48-49 0.4275813134137688 2.0954271998011514E-4 0.0 2.456707751491005E-5 1.9220125349900214E-4 50-51 0.5601727210061519 2.1676833101391219E-4 0.0 2.745732192842888E-5 1.9942686453279922E-4 52-53 0.7136519249750355 2.19658575427431E-4 0.0 3.179268854870712E-5 2.1098784218687454E-4 54-55 0.8913658283512745 2.19658575427431E-4 0.0 3.179268854870712E-5 2.2110369763419043E-4 56-57 1.098052986850833 2.3989028632206283E-4 0.0 3.179268854870712E-5 2.3410979749502518E-4 58-59 1.3373131998907488 2.630122416302135E-4 0.0 3.179268854870712E-5 2.4856101956261933E-4 60-61 1.6090380833055147 2.6590248604373227E-4 0.0 3.323781075546654E-5 2.601219972166946E-4 62-63 1.9078199897974373 2.774634636978076E-4 0.0 3.6128055168985364E-5 2.7312809707752937E-4 64-65 2.2372500480503135 2.948049301789206E-4 0.0 3.757317737574478E-5 2.7601834149104817E-4 66-67 2.597456873940545 3.1070127445327416E-4 0.0 3.757317737574478E-5 2.78908585904567E-4 68-69 2.993433364692485 3.179268854870712E-4 0.0 3.757317737574478E-5 2.8324395252484527E-4 70-71 3.422199678315797 3.2370737431410885E-4 0.0 4.335366620278244E-5 2.9046956355864237E-4 72-73 3.8823887292028854 3.367134741749436E-4 0.0 4.335366620278244E-5 3.0203054121271766E-4 74-75 4.374617584536036 3.4827445182901895E-4 0.0 4.335366620278244E-5 3.1359151886679296E-4 76-77 4.8983024149437195 3.55500062862816E-4 0.0 4.91341550298201E-5 3.179268854870712E-4 78-79 5.453603628990885 3.583903072763348E-4 0.0 5.202439944333892E-5 3.265976187276277E-4 80-81 6.043044410050536 3.7139640713716954E-4 0.0 5.491464385685775E-5 3.323781075546654E-4 82-83 6.662154995137163 3.872927514115231E-4 0.0 5.491464385685775E-5 3.4249396300198125E-4 84-85 7.309993164571962 4.0607934009939553E-4 0.0 5.491464385685775E-5 3.569451850695754E-4 86-87 7.984712052174692 4.1475007333995203E-4 0.0 5.491464385685775E-5 3.6995128493041014E-4 88 8.508235028895218 4.2486592878726787E-4 0.0 5.491464385685775E-5 3.757317737574478E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 212820 0.0 84.24209 8 GTGGTAT 212810 0.0 83.45115 6 TGGTATC 216065 0.0 82.91649 7 AGTGGTA 214885 0.0 82.69132 5 GTATCAA 216880 0.0 82.634415 9 GCAGTGG 215505 0.0 82.36648 3 AAGCAGT 209940 0.0 82.29068 1 CAGTGGT 230475 0.0 77.000626 4 AGCAGTG 230055 0.0 76.84349 2 CAACGCA 208260 0.0 44.85636 12-13 ATCAACG 209220 0.0 44.72233 10-11 ACGCAGA 211240 0.0 44.28172 14-15 AGAGTAC 212680 0.0 43.8567 18-19 TCAACGC 207545 0.0 43.11226 12-13 AACGCAG 209330 0.0 42.80442 14-15 GAGTACT 210050 0.0 42.290905 20-21 CGCAGAG 212800 0.0 42.131065 16-17 TATCAAC 215265 0.0 41.590115 10-11 AGTACTT 224485 0.0 41.26734 20-21 GTACTTT 221965 0.0 40.008846 22-23 >>END_MODULE