FastQCFastQC Report
Thu 26 May 2016
SRR1660712_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660712_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28387730
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15434425.437003945014272No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT365730.12883383067261805No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA301500.10620785811334685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1936400.086.091918
GTGGTAT1964300.084.918776
AGTGGTA1968100.084.78395
GTATCAA1964150.084.7672969
TGGTATC1964400.084.586027
AAGCAGT2042300.082.0371551
GCAGTGG2066800.081.272923
CAGTGGT2059750.080.8432164
AGCAGTG2157200.077.89112
CAACGCA1909900.045.1339712-13
ATCAACG1925150.044.85817710-11
ACGCAGA1941600.044.45462414-15
AGAGTAC1929800.043.9409218-19
TCAACGC1904450.043.90917212-13
AACGCAG1925650.043.54114-15
GAGTACT1906300.042.9470820-21
CGCAGAG1960450.042.7098816-17
AGTACTT1985300.042.37108220-21
TATCAAC1964000.042.3481810-11
GTACTTT1993050.041.0462722-23
Produced by FastQC (version 0.11.5)