##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660712_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28387730 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.71372554269045 34.0 31.0 34.0 30.0 34.0 2 31.841052102440035 34.0 31.0 34.0 30.0 34.0 3 31.898847882518258 34.0 31.0 34.0 30.0 34.0 4 35.29282693614459 37.0 35.0 37.0 33.0 37.0 5 35.25766590002089 37.0 35.0 37.0 33.0 37.0 6 35.3229036277293 37.0 35.0 37.0 33.0 37.0 7 35.26333232703002 37.0 35.0 37.0 33.0 37.0 8 35.27732932502881 37.0 35.0 37.0 32.0 37.0 9 36.92528877793328 39.0 37.0 39.0 33.0 39.0 10-11 36.86259082709326 39.0 37.0 39.0 33.0 39.0 12-13 36.88863781993136 39.0 37.0 39.0 33.0 39.0 14-15 38.23931182591916 40.0 38.0 41.0 33.0 41.0 16-17 38.21826016028756 40.0 38.0 41.0 33.0 41.0 18-19 38.18241379286051 40.0 38.0 41.0 33.0 41.0 20-21 38.06183581779875 40.0 38.0 41.0 32.5 41.0 22-23 37.886938952145876 40.0 38.0 41.0 32.5 41.0 24-25 37.870721258797374 40.0 38.0 41.0 32.0 41.0 26-27 37.85064230214955 40.0 38.0 41.0 32.0 41.0 28-29 37.76812566556043 40.0 38.0 41.0 32.0 41.0 30-31 37.67851878258671 40.0 38.0 41.0 32.0 41.0 32-33 37.55655385266803 40.0 37.5 41.0 31.0 41.0 34-35 37.405910317591434 40.0 37.0 41.0 31.0 41.0 36-37 37.21908291011645 40.0 37.0 41.0 30.0 41.0 38-39 36.80452042132288 39.0 35.0 41.0 30.0 41.0 40-41 36.74609045880034 39.0 35.0 41.0 30.0 41.0 42-43 36.75479402544691 39.0 35.0 41.0 30.0 41.0 44-45 36.76302844574047 39.5 35.0 41.0 30.0 41.0 46-47 36.868298680451026 40.0 35.0 41.0 30.0 41.0 48-49 36.76870348562566 40.0 35.0 41.0 30.0 41.0 50-51 35.415987928587455 38.5 34.0 40.0 28.0 40.5 52-53 35.60028202325441 38.5 34.5 39.5 28.0 40.5 54-55 35.99603349757096 39.0 35.0 41.0 27.0 41.0 56-57 35.628241761493435 39.0 35.0 41.0 24.5 41.0 58-59 35.44357974378367 39.0 35.0 41.0 24.5 41.0 60-61 35.21970574963197 39.0 35.0 41.0 24.0 41.0 62-63 34.987529488972875 38.0 34.5 41.0 24.0 41.0 64-65 34.74120153319762 37.5 34.0 40.0 23.5 41.0 66-67 34.491060274985 37.0 34.0 40.0 23.0 41.0 68-69 34.24013887337945 36.5 34.0 39.5 23.0 41.0 70-71 33.96595740483653 36.0 34.0 39.0 24.0 41.0 72-73 33.64457822094264 35.5 33.0 39.0 24.0 41.0 74-75 33.28508683152897 35.0 33.0 38.5 23.0 40.0 76-77 32.907720747661045 35.0 33.0 37.0 22.0 39.5 78-79 32.55119400177471 35.0 33.0 37.0 21.0 39.0 80-81 32.25068675797607 35.0 32.5 36.0 20.0 39.0 82-83 31.961856548586304 35.0 32.0 36.0 20.0 37.5 84-85 31.69564537213789 35.0 32.0 35.5 20.0 37.0 86-87 31.497365728080407 35.0 32.0 35.0 19.5 37.0 88-89 31.30698289366568 35.0 32.0 35.0 19.0 36.0 90-91 31.145351548010353 35.0 32.0 35.0 19.0 36.0 92-93 31.001575610307693 35.0 31.0 35.0 18.0 36.0 94-95 30.832218990387748 35.0 31.0 35.0 18.0 35.5 96-97 30.70941760753678 35.0 31.0 35.0 17.5 35.0 98-99 30.61777329501161 35.0 31.0 35.0 17.0 35.0 100 30.54790833927193 34.0 31.0 35.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5287508391356042 1101 2 0.5705393672115662 1101 3 0.5704942852037966 1101 4 0.5117334628453989 1101 5 0.5196660131445086 1101 6 0.4701291616606653 1101 7 0.49819677830208065 1101 8 0.5215636509451045 1101 9 0.6262126892605551 1101 10-11 0.615372047151098 1101 12-13 0.6009236169195802 1101 14-15 0.7269261635373425 1101 16-17 0.7158176972186467 1101 18-19 0.7031356384534675 1101 20-21 0.7499134835273011 1101 22-23 0.7884127000991441 1101 24-25 0.7764452401821629 1101 26-27 0.7562736582041225 1101 28-29 0.7232217669252705 1101 30-31 0.6950506186116954 1101 32-33 0.6799422303648512 1101 34-35 0.699618396232843 1101 36-37 0.6560710790597923 1101 38-39 0.6813977559112132 1101 40-41 0.6952592404158153 1101 42-43 0.6791727616212597 1101 44-45 0.7017918455227274 1101 46-47 0.7683194467966459 1101 48-49 0.7708407137316797 1101 50-51 0.6932459356410874 1101 52-53 0.7400589466226606 1101 54-55 0.7690722670985011 1101 56-57 0.7731764076887231 1101 58-59 0.7139378900928719 1101 60-61 0.6915413304543492 1101 62-63 0.6677568502716937 1101 64-65 0.6619251401170629 1101 66-67 0.6281063695688616 1101 68-69 0.5914806335003675 1101 70-71 0.5695720039308085 1101 72-73 0.5897512185070397 1101 74-75 0.5661828479429545 1101 76-77 0.5103348752813517 1101 78-79 0.5249962793044674 1101 80-81 0.4961434248464087 1101 82-83 0.48561701763146914 1101 84-85 0.47220532367321155 1101 86-87 0.44046537977325073 1101 88-89 0.44711782661965316 1101 90-91 0.42609589302619 1101 92-93 0.441402385201382 1101 94-95 0.4454576297798063 1101 96-97 0.45248465187031073 1101 98-99 0.4250714153120754 1101 100 0.40310752535756933 1102 1 0.45111826372037456 1102 2 0.5016309820278835 1102 3 0.5047451973241941 1102 4 0.4449254068163384 1102 5 0.4347516138187473 1102 6 0.3884720189136601 1102 7 0.4310079156855693 1102 8 0.4844295975976465 1102 9 0.5294373322509642 1102 10-11 0.5238247227155455 1102 12-13 0.5261959793862943 1102 14-15 0.6099906621272808 1102 16-17 0.6006210803741183 1102 18-19 0.565222666921251 1102 20-21 0.5796668570535459 1102 22-23 0.6090658823065809 1102 24-25 0.5508463817450107 1102 26-27 0.5572625904209403 1102 28-29 0.5813060904824923 1102 30-31 0.5706727778643597 1102 32-33 0.5823671352432953 1102 34-35 0.5656054299515176 1102 36-37 0.5648478882160006 1102 38-39 0.5949470844096325 1102 40-41 0.5497793772327029 1102 42-43 0.5881401824397727 1102 44-45 0.6145058895331843 1102 46-47 0.5961423376001065 1102 48-49 0.6154552528414925 1102 50-51 0.5779971778173021 1102 52-53 0.6140179567740134 1102 54-55 0.6850595567198283 1102 56-57 0.7361404796596531 1102 58-59 0.6346740394562502 1102 60-61 0.6352413698568427 1102 62-63 0.6114579640746456 1102 64-65 0.5612756188152019 1102 66-67 0.5858837310580682 1102 68-69 0.534604211482538 1102 70-71 0.5397212363296404 1102 72-73 0.5162019345474036 1102 74-75 0.5034217132864782 1102 76-77 0.521334248456597 1102 78-79 0.49982417728283934 1102 80-81 0.45887086500255947 1102 82-83 0.4551676168916643 1102 84-85 0.42565760802567 1102 86-87 0.39312342499058417 1102 88-89 0.37127943809113617 1102 90-91 0.37944750280102824 1102 92-93 0.36264894640368794 1102 94-95 0.4147819472828722 1102 96-97 0.4170313124435836 1102 98-99 0.349541135529865 1102 100 0.34290382189792723 1103 1 0.3910435931706715 1103 2 0.4207839319847082 1103 3 0.43798724136323486 1103 4 0.3726760490342329 1103 5 0.4018499640570994 1103 6 0.38067810412754 1103 7 0.3574933302042069 1103 8 0.39584958407389337 1103 9 0.46350450588132475 1103 10-11 0.42734961438693375 1103 12-13 0.47503031744806634 1103 14-15 0.5337003516976253 1103 16-17 0.524029326781033 1103 18-19 0.5004061599720231 1103 20-21 0.5350137350633233 1103 22-23 0.581295973916852 1103 24-25 0.6080833785342321 1103 26-27 0.5467012500634709 1103 28-29 0.5367719819834988 1103 30-31 0.49700112005964314 1103 32-33 0.43625078322144617 1103 34-35 0.4378055641258882 1103 36-37 0.4472143333891623 1103 38-39 0.45141000935020514 1103 40-41 0.4412818352472243 1103 42-43 0.48831838000754146 1103 44-45 0.520698775435001 1103 46-47 0.5726340573947439 1103 48-49 0.5825545291187879 1103 50-51 0.5092216840715835 1103 52-53 0.5206138247170813 1103 54-55 0.5865210207389211 1103 56-57 0.5815471808192854 1103 58-59 0.5048255747661798 1103 60-61 0.5171863015657152 1103 62-63 0.5135653242917826 1103 64-65 0.5068314201520678 1103 66-67 0.472280130455637 1103 68-69 0.4674056708250305 1103 70-71 0.440304001913411 1103 72-73 0.40329725461602806 1103 74-75 0.43972239857598083 1103 76-77 0.4631701255008167 1103 78-79 0.4297247321957869 1103 80-81 0.3569563559691318 1103 82-83 0.3851027317605116 1103 84-85 0.39748291417567216 1103 86-87 0.3494187250214864 1103 88-89 0.3248207013403679 1103 90-91 0.32363696636701533 1103 92-93 0.2721777256625941 1103 94-95 0.34950107057768776 1103 96-97 0.32380379380438384 1103 98-99 0.32363364655894244 1103 100 0.3107719766028403 1104 1 0.4557471923208354 1104 2 0.46350430662317166 1104 3 0.4532539938191569 1104 4 0.3962973702835413 1104 5 0.4371402976531584 1104 6 0.3384034344226805 1104 7 0.37258380787469036 1104 8 0.38878527366376403 1104 9 0.46408115792155513 1104 10-11 0.44333569949068874 1104 12-13 0.4411155477256514 1104 14-15 0.5359184374075596 1104 16-17 0.5159194438323951 1104 18-19 0.4941100278555055 1104 20-21 0.4348454348631421 1104 22-23 0.4986899157763176 1104 24-25 0.5186354651794503 1104 26-27 0.4867188466224093 1104 28-29 0.4653009674263373 1104 30-31 0.45012388204322207 1104 32-33 0.4196816265241168 1104 34-35 0.4317706516522577 1104 36-37 0.3908619251519099 1104 38-39 0.4251384707609347 1104 40-41 0.4241395825514118 1104 42-43 0.42908489260808835 1104 44-45 0.4459392002796534 1104 46-47 0.46164335649132937 1104 48-49 0.4912349912504297 1104 50-51 0.3803791952583353 1104 52-53 0.42239457519441714 1104 54-55 0.5682139948300247 1104 56-57 0.5997939041624392 1104 58-59 0.5140258916955602 1104 60-61 0.5440572372129466 1104 62-63 0.5262961082043915 1104 64-65 0.5083976719153043 1104 66-67 0.5088262130253653 1104 68-69 0.4866729199601352 1104 70-71 0.46995669827280295 1104 72-73 0.47466370717794604 1104 74-75 0.4615202814733763 1104 76-77 0.4326347484587032 1104 78-79 0.41632553240604153 1104 80-81 0.40810073632445665 1104 82-83 0.39655837729202403 1104 84-85 0.3977363450095446 1104 86-87 0.3678002446170616 1104 88-89 0.3642123644365505 1104 90-91 0.3765528589624161 1104 92-93 0.35990001623888546 1104 94-95 0.447866545255426 1104 96-97 0.41116136668149394 1104 98-99 0.3998739244521552 1104 100 0.36435682602475694 1105 1 0.29246227228688326 1105 2 0.35980196023071187 1105 3 0.31879554720608994 1105 4 0.3001647464171455 1105 5 0.3332029356409194 1105 6 0.2650172614514048 1105 7 0.29806232930712184 1105 8 0.2927287551510318 1105 9 0.34350505632617256 1105 10-11 0.3258851844471309 1105 12-13 0.3086684101039694 1105 14-15 0.33864393502409484 1105 16-17 0.3104485616671653 1105 18-19 0.34416608423484973 1105 20-21 0.38943742546981497 1105 22-23 0.4298022126094736 1105 24-25 0.4272464223731802 1105 26-27 0.3922627587442449 1105 28-29 0.38745765017085887 1105 30-31 0.39320723917905553 1105 32-33 0.3980972883114333 1105 34-35 0.40563166878798995 1105 36-37 0.36884234438415575 1105 38-39 0.38876607390718476 1105 40-41 0.37566845863016596 1105 42-43 0.4096722265856698 1105 44-45 0.4449419137643602 1105 46-47 0.4595506839458281 1105 48-49 0.4883774803260579 1105 50-51 0.34388037827513784 1105 52-53 0.4161971844329031 1105 54-55 0.5097371772370067 1105 56-57 0.4866771600750184 1105 58-59 0.4723270753169544 1105 60-61 0.44301662688422994 1105 62-63 0.4308957665377875 1105 64-65 0.42895182564718937 1105 66-67 0.39492460064854384 1105 68-69 0.3928550106723705 1105 70-71 0.421599105319693 1105 72-73 0.40679145695192886 1105 74-75 0.42098499939090317 1105 76-77 0.4190403591921168 1105 78-79 0.4072446583554594 1105 80-81 0.34168043231481704 1105 82-83 0.3369293370359543 1105 84-85 0.3361765695308243 1105 86-87 0.2893176696247579 1105 88-89 0.3391285048882793 1105 90-91 0.315156623526196 1105 92-93 0.2978852631023763 1105 94-95 0.35517691792083284 1105 96-97 0.370379165007126 1105 98-99 0.31179408649105866 1105 100 0.312691137330269 1106 1 0.27726533786202623 1106 2 0.32670135512993426 1106 3 0.3004566260775725 1106 4 0.2795465355702973 1106 5 0.29211762200613833 1106 6 0.2714821649481536 1106 7 0.28091100793250234 1106 8 0.2936466118361949 1106 9 0.30089020782455833 1106 10-11 0.2892309416551768 1106 12-13 0.2975970507007233 1106 14-15 0.3683867229679123 1106 16-17 0.3738299353483754 1106 18-19 0.32909756736120954 1106 20-21 0.3435594148023924 1106 22-23 0.3361315966022431 1106 24-25 0.3290767887084698 1106 26-27 0.36612786051970403 1106 28-29 0.3358965987815239 1106 30-31 0.3416529824711816 1106 32-33 0.35174143990217743 1106 34-35 0.3607025749210493 1106 36-37 0.31465488826352583 1106 38-39 0.33848832764221726 1106 40-41 0.3671254805535469 1106 42-43 0.366578981082732 1106 44-45 0.3692744999098707 1106 46-47 0.3524793863053972 1106 48-49 0.36614031094742217 1106 50-51 0.26842896072331257 1106 52-53 0.30584683780001143 1106 54-55 0.43041412061618445 1106 56-57 0.42103225365109154 1106 58-59 0.3490950810042719 1106 60-61 0.37846849518635395 1106 62-63 0.3408560418857931 1106 64-65 0.3121531411695173 1106 66-67 0.33208185171661597 1106 68-69 0.32847831193772237 1106 70-71 0.37138733624743026 1106 72-73 0.34998585128443693 1106 74-75 0.3327866976987153 1106 76-77 0.3409187578185069 1106 78-79 0.3493542743995164 1106 80-81 0.33121972128438415 1106 82-83 0.3034437814102233 1106 84-85 0.3149440603394247 1106 86-87 0.2886850661383562 1106 88-89 0.294719694630448 1106 90-91 0.26927277856084686 1106 92-93 0.24613784587843668 1106 94-95 0.2757933352803299 1106 96-97 0.36911152171995454 1106 98-99 0.32716247959222144 1106 100 0.27713357026901164 1107 1 0.21282764240412888 1107 2 0.2354086688088728 1107 3 0.225484225704502 1107 4 0.24643545751111162 1107 5 0.26145298827019303 1107 6 0.22304978074530624 1107 7 0.23325699714693116 1107 8 0.2406755811909349 1107 9 0.2897304377709631 1107 10-11 0.2603924854719821 1107 12-13 0.2734986212168238 1107 14-15 0.3037972626436769 1107 16-17 0.30264589060903546 1107 18-19 0.2508167739432423 1107 20-21 0.24218877448857512 1107 22-23 0.26117045854422827 1107 24-25 0.30805574841058814 1107 26-27 0.24956163444699797 1107 28-29 0.30401965805215525 1107 30-31 0.2609178534891399 1107 32-33 0.2247210276328886 1107 34-35 0.2832648330978884 1107 36-37 0.24958630116397273 1107 38-39 0.22675455279419765 1107 40-41 0.26267681723854963 1107 42-43 0.29735948034513626 1107 44-45 0.2998387653841945 1107 46-47 0.2692918328813718 1107 48-49 0.34425723362920735 1107 50-51 0.26299915804966645 1107 52-53 0.2547103017173171 1107 54-55 0.2951234508724454 1107 56-57 0.36413120242314534 1107 58-59 0.2896346695863343 1107 60-61 0.30499156115511283 1107 62-63 0.2836412303741298 1107 64-65 0.2586357461193103 1107 66-67 0.29024123120942846 1107 68-69 0.2779392107643801 1107 70-71 0.24080380375944088 1107 72-73 0.2443331592795559 1107 74-75 0.26393054118654646 1107 76-77 0.2540953720280612 1107 78-79 0.26989247889373047 1107 80-81 0.1965008665278276 1107 82-83 0.2436910517726858 1107 84-85 0.21376878871490845 1107 86-87 0.23934898729186216 1107 88-89 0.19065575470730423 1107 90-91 0.21486403374805008 1107 92-93 0.21094497768676845 1107 94-95 0.2177868583762823 1107 96-97 0.26249932183936764 1107 98-99 0.21753333762396565 1107 100 0.22588117059516932 1108 1 0.09677137956383675 1108 2 0.13487255063773063 1108 3 0.1640141600586169 1108 4 0.121836093939379 1108 5 0.147948569746589 1108 6 0.09240788742730643 1108 7 0.12256390186412602 1108 8 0.1402271152641461 1108 9 0.13450387751741033 1108 10-11 0.10205985451855781 1108 12-13 0.1035938462841628 1108 14-15 0.15270104683738595 1108 16-17 0.12406389241228766 1108 18-19 0.12860724150292668 1108 20-21 0.111479272450147 1108 22-23 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-1.1367560856190764 2316 80-81 -1.2090357072641922 2316 82-83 -1.1838638337257663 2316 84-85 -1.2592199362444916 2316 86-87 -1.3940631212064076 2316 88-89 -1.3781858158524436 2316 90-91 -1.3098686883386463 2316 92-93 -1.1723360969813008 2316 94-95 -1.3913992408390676 2316 96-97 -1.5436449506157466 2316 98-99 -1.4356649922110272 2316 100 -1.2324532347465897 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14537.0 3 3686.0 4 2616.0 5 2275.0 6 3981.0 7 20327.0 8 38700.0 9 43667.0 10 44679.0 11 44256.0 12 46892.0 13 50986.0 14 59262.0 15 67700.0 16 76790.0 17 87795.0 18 100152.0 19 113442.0 20 131388.0 21 157697.0 22 197064.0 23 270726.0 24 406648.0 25 532400.0 26 531226.0 27 451042.0 28 408068.0 29 399546.0 30 446187.0 31 535620.0 32 666391.0 33 860342.0 34 1192358.0 35 1821245.0 36 3218681.0 37 5983703.0 38 7236615.0 39 2115218.0 40 3822.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 14.455623751161891 29.68412069152069 29.50591117736171 26.354344379955712 2 21.233923594702667 34.493839120361244 26.128275147139803 18.14396213779629 3 25.8303594222976 24.68645800270682 26.85538791168018 22.627794663315395 4 18.778144402540438 28.283277196269125 23.157332979882973 29.781245421307467 5 23.152325694352452 32.290899456881704 25.603433567359918 18.953341281405926 6 30.17092753061438 27.5645653193772 24.414942249552237 17.849564900456187 7 21.997015312450348 24.79808595076119 31.90939364547844 21.295505091310023 8 28.371853122819108 26.292294949689794 24.47026286456596 20.865589062925142 9 26.604179461771864 24.403891106501355 27.37712227958764 21.614807152139136 10-11 21.339843418485003 29.563402942127396 28.21916304508627 20.877590594301328 12-13 21.01752364641012 26.397392249706332 28.50505777113796 24.08002633274559 14-15 20.92044738438076 32.52138515345017 25.62704020050442 20.93112726166465 16-17 24.141365558017974 25.956531713505466 25.771512731831116 24.130589996645448 18-19 20.787207555279974 29.124696353518466 25.92902314818139 24.159072943020174 20-21 23.96788748715222 29.383896387011603 25.843493168352587 20.804722957483595 22-23 24.066300973321038 26.16444049445278 28.875121394277244 20.894137137948945 24-25 20.97617694061218 29.028711835241523 25.703104871593734 24.292006352552566 26-27 21.05364914146403 25.88220197500135 32.154598916149965 20.90954996738465 28-29 20.988890520518165 26.004790031791618 32.14032240567404 20.865997042016176 30-31 20.858201627597996 25.822724978860634 32.28967632007794 21.029397073463432 32-33 21.101063629553753 26.08105814782696 31.905386700209405 20.912491522409887 34-35 21.123992053503997 26.221031808834976 31.739707259302392 20.915268878358635 36-37 20.9564356867171 26.127981053113075 31.93885761392192 20.976725646247907 38-39 20.984082769352533 26.128705486317905 31.996980857696293 20.89023088663327 40-41 21.018820734053516 26.11320474081093 31.962198158263313 20.90577636687224 42-43 20.97139122765832 26.118589180961887 31.955486185574568 20.954533405805222 44-45 20.99889075157961 26.215544931505285 31.895289529418243 20.890274787496864 46-47 20.980316559182032 26.25759155385256 31.87099911800413 20.891092768961276 48-49 21.003330346495517 26.249939444078663 31.817798145095445 20.92893206433038 50-51 21.000337700329517 26.33562737065931 31.7657672149857 20.898267714025472 52-53 21.08583165525318 26.394886475186773 31.541766894668342 20.977514974891697 54-55 21.33049343483294 26.661927507581236 30.84422041317928 21.16335864440655 56-57 21.808783647122084 27.191226456145827 29.71840817001377 21.28158172671831 58-59 21.85501135649055 27.6850567711682 29.06914757061943 21.390784301721823 60-61 21.85078652801147 27.96553816601936 28.78722919119391 21.396446114775262 62-63 21.783148005941943 28.17549229398425 28.65516184097453 21.386197859099273 64-65 21.81917959975695 28.168866482586797 28.658901281377446 21.353052636278804 66-67 21.705915004137676 27.96601329435349 29.011589463098403 21.31648223841043 68-69 21.724998970489693 27.816372044478726 29.273038743184397 21.185590241847184 70-71 21.708811434825616 27.67028196429192 29.46526586008359 21.155640740798873 72-73 21.6968704231506 27.619369205067347 29.536017096385418 21.14774327539664 74-75 21.81172976826712 27.78102128978981 29.35186836166433 21.055380580278737 76-77 21.985949267004184 27.89979205030842 29.032387755808458 21.08187092687894 78-79 22.080379808819647 28.024261159084705 28.781679580021237 21.113679452074408 80-81 22.256235775087564 28.19990562877408 28.471260371785107 21.072598224353246 82-83 22.356043568665605 28.295207732786963 28.206585440380056 21.142163258167372 84-85 22.373446192820065 28.36045676217304 28.1064121768872 21.159684868119687 86-87 22.426492062873958 28.486106008377327 27.977919577341265 21.10948235140745 88-89 22.577486555491365 28.500389949218942 27.79573342042064 21.12639007486905 90-91 22.66426556137507 28.532390976822935 27.689087262197575 21.114256199604416 92-93 22.777063247324925 28.609110446421504 27.595523369161533 21.01830293709204 94-95 22.904684309155527 28.64481895483354 27.44239561764149 21.00810111836944 96-97 22.93587571571037 28.648359709479976 27.38728409291361 21.028480481896043 98-99 23.072344785758638 28.69185078972918 27.323583660915702 20.91222076359648 100 23.08904520372882 28.654757951727365 27.230275930040282 21.025920914503534 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 34382.0 1 24805.0 2 13152.5 3 10299.0 4 9329.5 5 9113.5 6 9192.0 7 9402.5 8 9412.0 9 9417.5 10 10087.0 11 13447.5 12 122985.0 13 153267.0 14 73897.0 15 75264.0 16 84361.0 17 93211.0 18 101495.5 19 110157.5 20 120565.5 21 136791.0 22 158841.0 23 183210.0 24 207613.5 25 236707.0 26 269556.5 27 306147.5 28 355824.5 29 407890.5 30 446158.0 31 489279.5 32 539741.0 33 576351.5 34 610841.0 35 659347.0 36 709027.0 37 743865.0 38 775780.5 39 802563.0 40 821582.0 41 853899.0 42 895717.0 43 904242.0 44 887332.5 45 889130.5 46 897987.0 47 898007.5 48 895939.0 49 898134.5 50 900155.0 51 898616.5 52 890116.5 53 866328.0 54 829979.5 55 787992.5 56 741255.5 57 686553.0 58 621259.0 59 540286.0 60 452612.0 61 373255.5 62 299775.0 63 231031.5 64 173179.0 65 127791.5 66 93844.5 67 70873.5 68 54241.5 69 41430.5 70 32054.5 71 25628.5 72 21420.5 73 17760.5 74 15179.5 75 13761.5 76 11025.5 77 7157.0 78 4720.5 79 3589.5 80 2842.0 81 2300.5 82 1916.0 83 1617.0 84 1359.5 85 1150.5 86 980.5 87 850.0 88 715.0 89 604.5 90 526.0 91 440.0 92 416.0 93 361.0 94 284.0 95 245.0 96 204.5 97 170.0 98 132.5 99 97.5 100 62.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40845463867663956 2 0.3876005584102709 3 0.16987973325095032 4 0.1759422116527105 5 0.2782187938239514 6 0.10272748120402724 7 0.13750306910767432 8 0.1433682791825905 9 0.13623139292926909 10-11 0.13304339586152186 12-13 0.13469375677449377 14-15 0.14099753661176853 16-17 0.1450591505555393 18-19 0.15686883030097862 20-21 0.13726881296954704 22-23 0.09925591091644172 24-25 0.0945725494782429 26-27 0.08918994227435587 28-29 0.09937568097202558 30-31 0.10391461381378504 32-33 0.10524793634432905 34-35 0.10594013681262995 36-37 0.10172000367764523 38-39 0.10103837115542524 40-41 0.10199124762705578 42-43 0.06379516784188098 44-45 0.06683169101580154 46-47 0.08531326738700136 48-49 0.0898839040670036 50-51 0.09957471062321643 52-53 0.10454692925429403 54-55 0.10701278333984438 56-57 0.1076820865916366 58-59 0.10701102201549755 60-61 0.10500311226012084 62-63 0.10927432380116338 64-65 0.11627911072847319 66-67 0.12493954254179535 68-69 0.12988886395636426 70-71 0.1283829316398317 72-73 0.11529805306729352 74-75 0.11264725992532688 76-77 0.11467806689721227 78-79 0.11343281058400936 80-81 0.10768560924033024 82-83 0.11072917771163808 84-85 0.11646404978488945 86-87 0.13003505387715045 88-89 0.1379715813839289 90-91 0.14464876198272986 92-93 0.14383503013449825 94-95 0.1340860998748403 96-97 0.13051765674817958 98-99 0.12576031968741425 100 0.11971721585346909 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.838773E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.52902481436514 #Duplication Level Percentage of deduplicated Percentage of total 1 64.25938834133129 24.115921796177478 2 17.441036623815066 13.090901924868136 3 7.1016765314805 7.995569843205787 4 3.374622866470378 5.065852211795633 5 1.8712012957419422 3.5112179930285774 6 1.198901403807192 2.6996160320074383 7 0.8438507263553087 2.2168226394305894 8 0.585355415628635 1.7574254334680048 9 0.4634137167384905 1.5652318387316388 >10 2.534583499430119 17.097672614781064 >50 0.18112626580396302 4.735707987141644 >100 0.14313481883110102 9.697085947473393 >500 0.001106802735407854 0.2579963862029765 >1k 5.360241419391716E-4 0.43296544481932886 >5k 3.752439345414927E-5 0.09120908401560449 >10k+ 2.8143295082945595E-5 5.668802822852885 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1543442 5.437003945014272 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 36573 0.12883383067261805 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 30150 0.10620785811334685 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.002060749485781357 2.4658540855503416E-5 0.0 0.0 0.0 2 0.0020642721344750004 2.818118954914676E-5 0.0 0.0 0.0 3 0.002081885377943217 2.818118954914676E-5 0.0 3.522648693643345E-6 1.7613243468216727E-5 4 0.0020854080266368602 5.988502779193687E-5 0.0 3.522648693643345E-6 1.7613243468216727E-5 5 0.0020854080266368602 9.158886603472697E-5 0.0 3.522648693643345E-6 2.4658540855503416E-5 6 0.0020854080266368602 9.511151472837032E-5 0.0 1.0567946080930036E-5 3.1703838242790106E-5 7 0.0020854080266368602 1.0567946080930036E-4 0.0 1.0567946080930036E-5 3.1703838242790106E-5 8 0.0020854080266368602 1.092021095029437E-4 0.0 1.0567946080930036E-5 3.1703838242790106E-5 9 0.002092453324024147 1.162474068902304E-4 0.0 1.409059477457338E-5 3.1703838242790106E-5 10-11 0.0020959759727177904 1.2329270427751708E-4 0.0 1.9374567815038397E-5 3.5226486936433454E-5 12-13 0.0021030212701050773 1.3914462339891214E-4 0.0 2.113589216186007E-5 3.5226486936433454E-5 14-15 0.0021065439187987205 1.4971256947984217E-4 0.0 2.2897216508681746E-5 3.6987811283255124E-5 16-17 0.0021100665674923637 1.6732581294805887E-4 0.0 2.4658540855503416E-5 4.931708171100683E-5 18-19 0.002125918486613759 1.99029651190849E-4 0.0 2.6419865202325087E-5 6.340767648558021E-5 20-21 0.002134725108347867 2.3601746247410414E-4 0.0 2.818118954914676E-5 7.397562256651025E-5 22-23 0.002180519541365231 2.6595997637007254E-4 0.0 2.818118954914676E-5 9.158886603472697E-5 24-25 0.0023496066786601114 2.888571928787543E-4 0.0 2.818118954914676E-5 9.6872839075192E-5 26-27 0.0026613610880475472 3.223223554683661E-4 0.0 3.1703838242790106E-5 1.02156812115657E-4 28-29 0.0036617933170422575 3.540261937111562E-4 0.0 3.5226486936433454E-5 1.092021095029437E-4 30-31 0.005734872073251366 3.98059302381698E-4 0.0 3.6987811283255124E-5 1.1448608254340873E-4 32-33 0.009741884962270672 4.156725458499147E-4 0.0 3.8749135630076795E-5 1.2153137993069541E-4 34-35 0.017076039542436115 4.491377084395265E-4 0.0 3.8749135630076795E-5 1.285766773179821E-4 36-37 0.028308004902117923 4.614669788672782E-4 0.0 3.8749135630076795E-5 1.3033800166480376E-4 38-39 0.04474644503100459 4.667509519077432E-4 0.0 3.8749135630076795E-5 1.3914462339891214E-4 40-41 0.06638079198301519 4.8612551972278164E-4 0.0 4.0510459976898466E-5 1.4971256947984217E-4 42-43 0.09308070775648493 5.195906823123933E-4 0.0 4.227178432372014E-5 1.5499654252030718E-4 44-45 0.1266180846443164 5.442492231678968E-4 0.0 4.4033108670541814E-5 1.5499654252030718E-4 46-47 0.1691646355661407 6.041342509598337E-4 0.0 4.931708171100683E-5 1.7084846164170224E-4 48-49 0.22243765176010905 6.323154405089805E-4 0.0 4.931708171100683E-5 1.7437111033534558E-4 50-51 0.28336890621405797 6.446447109367322E-4 0.0 5.283973040465018E-5 1.796550833758106E-4 52-53 0.35084524194079625 6.587353057113056E-4 0.0 5.988502779193687E-5 1.8141640772263227E-4 54-55 0.4274769416223136 6.93961792647739E-4 0.0 5.988502779193687E-5 1.8317773206945394E-4 56-57 0.5186360445164161 7.379949013182808E-4 0.0 5.988502779193687E-5 1.867003807630973E-4 58-59 0.626333983027174 7.608921178269626E-4 0.0 5.988502779193687E-5 1.867003807630973E-4 60-61 0.7475113367641584 7.679374152142493E-4 0.0 5.988502779193687E-5 1.99029651190849E-4 62-63 0.8790875494447777 7.890733073761093E-4 0.0 5.988502779193687E-5 2.0783627292495735E-4 64-65 1.0219098180798536 8.366290647402945E-4 0.0 5.988502779193687E-5 2.2192686769953075E-4 66-67 1.1826341873760247 8.454356864744029E-4 0.0 5.988502779193687E-5 2.324948137804608E-4 68-69 1.3608449847874415 8.489583351680462E-4 0.0 6.164635213875854E-5 2.324948137804608E-4 70-71 1.5556245603294099 8.61287605595798E-4 0.0 6.693032517922357E-5 2.3601746247410414E-4 72-73 1.7680297086100227 8.947527681854097E-4 0.0 7.045297387286691E-5 2.4306275986139082E-4 74-75 1.9949763506979952 9.194113090409131E-4 0.0 7.573694691333192E-5 2.501080572486775E-4 76-77 2.2413627296018386 9.493538229368816E-4 0.0 9.158886603472698E-5 2.677213007168942E-4 78-79 2.5060915402534825 9.986709046478884E-4 0.0 1.0391813646247868E-4 2.712439494105376E-4 80-81 2.789617204334408 0.0010286134185438567 0.0 1.2329270427751708E-4 2.7476659810418095E-4 82-83 3.0908318488304634 0.001070885202867577 0.0 1.2329270427751708E-4 2.835732198382893E-4 84-85 3.4120850804203084 0.0010920210950294369 0.0 1.2681535297116042E-4 2.888571928787543E-4 86-87 3.7530352021806603 0.0011219636089254055 0.0 1.3033800166480376E-4 2.9237984157239765E-4 88 4.021212685903381 0.001123724933272227 0.0 1.3738329905209047E-4 2.9237984157239765E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 193640 0.0 86.09191 8 GTGGTAT 196430 0.0 84.91877 6 AGTGGTA 196810 0.0 84.7839 5 GTATCAA 196415 0.0 84.767296 9 TGGTATC 196440 0.0 84.58602 7 AAGCAGT 204230 0.0 82.037155 1 GCAGTGG 206680 0.0 81.27292 3 CAGTGGT 205975 0.0 80.843216 4 AGCAGTG 215720 0.0 77.8911 2 CAACGCA 190990 0.0 45.13397 12-13 ATCAACG 192515 0.0 44.858177 10-11 ACGCAGA 194160 0.0 44.454624 14-15 AGAGTAC 192980 0.0 43.94092 18-19 TCAACGC 190445 0.0 43.909172 12-13 AACGCAG 192565 0.0 43.541 14-15 GAGTACT 190630 0.0 42.94708 20-21 CGCAGAG 196045 0.0 42.70988 16-17 AGTACTT 198530 0.0 42.371082 20-21 TATCAAC 196400 0.0 42.34818 10-11 GTACTTT 199305 0.0 41.04627 22-23 >>END_MODULE