FastQCFastQC Report
Thu 26 May 2016
SRR1660712_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660712_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28387730
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15642375.510257424598585No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT389420.13717898542785914No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT347760.12250363097014098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2029850.085.758618
GTGGTAT2030300.084.958986
GTATCAA2059250.084.438069
TGGTATC2063000.084.372127
AGTGGTA2046550.084.314215
AAGCAGT2005800.083.760471
GCAGTGG2086150.082.722813
CAGTGGT2139750.080.464344
AGCAGTG2169300.079.257562
CAACGCA1991000.045.0889612-13
ATCAACG2009450.044.75793510-11
ACGCAGA2026100.044.40298514-15
AGAGTAC2046350.043.87688418-19
TCAACGC1985850.043.75461612-13
AACGCAG2007900.043.3525814-15
GAGTACT2021900.042.7401320-21
CGCAGAG2052050.042.4498116-17
AGTACTT2100450.042.43462820-21
TATCAAC2052900.042.31871410-11
GTACTTT2101550.041.09317422-23
Produced by FastQC (version 0.11.5)