##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660712_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28387730 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.254243505909066 34.0 31.0 34.0 27.0 34.0 2 31.89713235260445 34.0 31.0 34.0 28.0 34.0 3 31.66221941662824 34.0 31.0 34.0 30.0 34.0 4 35.5872741850088 37.0 35.0 37.0 33.0 37.0 5 35.92307031946549 37.0 35.0 37.0 35.0 37.0 6 35.367963518041066 37.0 35.0 37.0 33.0 37.0 7 35.57471544924515 37.0 35.0 37.0 33.0 37.0 8 35.9449656946857 37.0 35.0 37.0 35.0 37.0 9 37.749541544885766 39.0 38.0 39.0 35.0 39.0 10-11 37.76596899435073 39.0 37.5 39.0 35.0 39.0 12-13 37.8474030857698 39.0 38.0 39.0 35.0 39.0 14-15 39.30041158627337 41.0 39.0 41.0 36.0 41.0 16-17 39.27089295269471 41.0 39.0 41.0 36.0 41.0 18-19 39.26652715803624 41.0 39.0 41.0 36.0 41.0 20-21 39.20364662831442 40.5 39.0 41.0 36.0 41.0 22-23 39.07976058670418 40.0 39.0 41.0 35.5 41.0 24-25 39.09627985400735 40.0 39.0 41.0 36.0 41.0 26-27 39.05396592119201 40.0 39.0 41.0 35.5 41.0 28-29 38.979844778007966 40.0 39.0 41.0 35.0 41.0 30-31 38.891760419026106 40.0 38.0 41.0 35.0 41.0 32-33 38.78069005517524 40.0 38.0 41.0 35.0 41.0 34-35 38.70278000389605 40.0 38.0 41.0 35.0 41.0 36-37 38.58029299982774 40.0 38.0 41.0 35.0 41.0 38-39 38.18030686145035 40.0 37.0 41.0 34.0 41.0 40-41 38.0554253193193 40.0 37.0 41.0 33.5 41.0 42-43 37.89780686937631 40.0 37.0 41.0 33.0 41.0 44-45 37.777469931551416 40.0 36.0 41.0 33.0 41.0 46-47 37.63256352304323 40.0 36.0 41.0 33.0 41.0 48-49 37.684374675255825 40.0 36.0 41.0 33.0 41.0 50-51 37.69309485119099 40.0 36.0 41.0 33.0 41.0 52-53 37.445516636941385 40.0 36.0 41.0 32.5 41.0 54-55 36.91072067051504 39.5 35.5 41.0 31.0 41.0 56-57 36.39646551520674 39.0 35.0 41.0 29.5 41.0 58-59 36.25083204962144 39.0 35.0 41.0 29.0 41.0 60-61 36.098520505161915 39.0 35.0 41.0 29.0 41.0 62-63 35.856927535241454 38.5 35.0 41.0 29.0 41.0 64-65 35.5929408409901 38.0 35.0 40.0 28.0 41.0 66-67 35.325036679579526 37.0 35.0 40.0 28.0 41.0 68-69 35.07920517068466 37.0 35.0 40.0 28.0 41.0 70-71 34.80161934399123 36.0 34.0 39.0 28.0 41.0 72-73 34.47659893904867 36.0 34.0 39.0 28.0 41.0 74-75 34.11554462086261 35.0 34.0 38.5 27.0 40.0 76-77 32.43438010013482 34.5 32.0 36.0 25.0 39.0 78-79 33.38326757722439 35.0 33.5 37.0 26.5 39.0 80-81 33.29814257779682 35.0 34.0 36.5 27.0 39.0 82-83 33.05961735933095 35.0 34.0 36.0 26.0 37.5 84-85 32.827357083500516 35.0 34.0 36.0 26.0 37.0 86-87 32.63022490702849 35.0 34.0 35.0 26.0 37.0 88-89 32.470999794629584 35.0 34.0 35.0 26.0 36.0 90-91 32.32067520016571 35.0 33.0 35.0 25.0 36.0 92-93 32.18904357622114 35.0 33.0 35.0 25.0 36.0 94-95 32.09268874263634 35.0 33.0 35.0 25.0 36.0 96-97 32.00528525880724 35.0 33.0 35.0 25.0 35.5 98-99 31.925227853724127 35.0 33.0 35.0 24.5 35.0 100 31.878386154863385 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.5468921773829543 1101 2 1.1733629802268801 1101 3 1.5422690717597511 1101 4 0.9569366552335339 1101 5 0.5821980110982565 1101 6 1.1273135267273489 1101 7 0.8097305025316217 1101 8 0.48715557896479567 1101 9 0.5225325829632013 1101 10-11 0.43726841607140443 1101 12-13 0.39238961084542723 1101 14-15 0.509418277975378 1101 16-17 0.5372983535066709 1101 18-19 0.5163173646166257 1101 20-21 0.5196334654360868 1101 22-23 0.5117990206165004 1101 24-25 0.5011758380379376 1101 26-27 0.51151223104889 1101 28-29 0.49881193802414003 1101 30-31 0.5247148318515258 1101 32-33 0.5527864361028918 1101 34-35 0.553538132618705 1101 36-37 0.5214553368570662 1101 38-39 0.5510739351726244 1101 40-41 0.5507589879308057 1101 42-43 0.5382534993233463 1101 44-45 0.4983775910542647 1101 46-47 0.5600878306404695 1101 48-49 0.5019526108339676 1101 50-51 0.47941761933743265 1101 52-53 0.4735882343986333 1101 54-55 0.46751067981152516 1101 56-57 0.4971062505290149 1101 58-59 0.4840385948594417 1101 60-61 0.5032259331618931 1101 62-63 0.4604234714570765 1101 64-65 0.4067315768260613 1101 66-67 0.4312436199406875 1101 68-69 0.3980608457673682 1101 70-71 0.3837406094727456 1101 72-73 0.34521673386207397 1101 74-75 0.3523052336892647 1101 76-77 0.3160986573394382 1101 78-79 0.4232729045802799 1101 80-81 0.40899451133713427 1101 82-83 0.42895652643060345 1101 84-85 0.39076317648787295 1101 86-87 0.3815136363314622 1101 88-89 0.37584280012009685 1101 90-91 0.37725605886989655 1101 92-93 0.3769176080078722 1101 94-95 0.33665688595096555 1101 96-97 0.3512325553513733 1101 98-99 0.37361756461993423 1101 100 0.38057811282115495 1102 1 1.4648895074522592 1102 2 1.1312872969068941 1102 3 1.4857334625866763 1102 4 0.9093203343901095 1102 5 0.4922175662282342 1102 6 1.0639454860488016 1102 7 0.7763800968666672 1102 8 0.4540996046028809 1102 9 0.4447567997356927 1102 10-11 0.3603671329481628 1102 12-13 0.34251547002067184 1102 14-15 0.4336740394835843 1102 16-17 0.46069732949495545 1102 18-19 0.4327028986702217 1102 20-21 0.42097501653069 1102 22-23 0.4419996807229296 1102 24-25 0.4134439114459738 1102 26-27 0.4251288821304513 1102 28-29 0.4127713781961262 1102 30-31 0.4133035133592813 1102 32-33 0.4413016579219189 1102 34-35 0.41795538914641384 1102 36-37 0.42590374800242614 1102 38-39 0.4260050791270231 1102 40-41 0.4590306074854169 1102 42-43 0.46065040806098523 1102 44-45 0.4002850353757097 1102 46-47 0.4396424355259967 1102 48-49 0.4292105597433462 1102 50-51 0.3794310031112431 1102 52-53 0.3625433548296826 1102 54-55 0.4050486321933704 1102 56-57 0.4195649958960104 1102 58-59 0.41905920094839644 1102 60-61 0.42297966703553413 1102 62-63 0.3762748363988422 1102 64-65 0.3322804443871519 1102 66-67 0.34858103372102534 1102 68-69 0.30127414569366096 1102 70-71 0.3043716629378821 1102 72-73 0.2944159128846948 1102 74-75 0.36470686223550786 1102 76-77 0.18355578342217171 1102 78-79 0.38493973568447615 1102 80-81 0.4003473132927482 1102 82-83 0.3787435099376566 1102 84-85 0.36255360440555506 1102 86-87 0.3186016070158857 1102 88-89 0.34770124084182186 1102 90-91 0.3384429227369239 1102 92-93 0.3193835031516983 1102 94-95 0.29368183504001877 1102 96-97 0.29801937328354455 1102 98-99 0.3522620618169299 1102 100 0.33791590452999287 1103 1 1.4722548775687052 1103 2 1.093823684307143 1103 3 1.4220675613076743 1103 4 0.8926769907264216 1103 5 0.4767612950097728 1103 6 1.0223463599084184 1103 7 0.7553609165898933 1103 8 0.4264029171545829 1103 9 0.4068632252769433 1103 10-11 0.3384134516462325 1103 12-13 0.302344148436525 1103 14-15 0.3562814902913587 1103 16-17 0.3921057784078883 1103 18-19 0.3478811556626269 1103 20-21 0.3722182449507301 1103 22-23 0.3472474473237952 1103 24-25 0.34904631167079714 1103 26-27 0.35172843361010564 1103 28-29 0.32603139949460314 1103 30-31 0.3373770708497119 1103 32-33 0.36311993347475635 1103 34-35 0.39032083450162247 1103 36-37 0.32286334895889723 1103 38-39 0.3689935614165165 1103 40-41 0.39182728457424787 1103 42-43 0.35237093076030845 1103 44-45 0.3641678058521336 1103 46-47 0.3644380129610596 1103 48-49 0.3386422250395782 1103 50-51 0.34578275307729456 1103 52-53 0.34585051293981195 1103 54-55 0.3246481427630883 1103 56-57 0.3860513408328785 1103 58-59 0.372726008620333 1103 60-61 0.33684076515945804 1103 62-63 0.32425260668343014 1103 64-65 0.3060001312245646 1103 66-67 0.33157393770203925 1103 68-69 0.30526016096676045 1103 70-71 0.2976686661262349 1103 72-73 0.2881382279696538 1103 74-75 0.2789731305288399 1103 76-77 0.12240943643627133 1103 78-79 0.3088514887773499 1103 80-81 0.32755744376103024 1103 82-83 0.32771137399588923 1103 84-85 0.31962133344031685 1103 86-87 0.3046494678592282 1103 88-89 0.32666147938751067 1103 90-91 0.3364738309304798 1103 92-93 0.2981441172002519 1103 94-95 0.276592634796323 1103 96-97 0.31404594915974826 1103 98-99 0.31677811520232524 1103 100 0.27833181869751655 1104 1 1.4231805775921949 1104 2 1.0714912924153843 1104 3 1.4099116815868946 1104 4 0.8621875103298109 1104 5 0.488048203127569 1104 6 1.014896423710411 1104 7 0.7599637090398517 1104 8 0.4119648172537538 1104 9 0.43119184394834775 1104 10-11 0.31612999663607155 1104 12-13 0.2959371274440201 1104 14-15 0.3437609144231786 1104 16-17 0.3707820013641907 1104 18-19 0.3389358773290283 1104 20-21 0.35234897159712375 1104 22-23 0.37053914936517174 1104 24-25 0.35585151810327176 1104 26-27 0.3328748433661346 1104 28-29 0.3149344156154186 1104 30-31 0.3140790914966516 1104 32-33 0.3530616657982435 1104 34-35 0.36409117818183745 1104 36-37 0.3543835261452415 1104 38-39 0.3955907730849617 1104 40-41 0.331202136285512 1104 42-43 0.34424441559377783 1104 44-45 0.35112724759972735 1104 46-47 0.3243002305243863 1104 48-49 0.3003892884078567 1104 50-51 0.30139620764960995 1104 52-53 0.3066942713016232 1104 54-55 0.3345207376464643 1104 56-57 0.39249589925839956 1104 58-59 0.35297268737144805 1104 60-61 0.3556998281157675 1104 62-63 0.31266141705315675 1104 64-65 0.2993052640979812 1104 66-67 0.31252510999020444 1104 68-69 0.2518027026097016 1104 70-71 0.275290970980997 1104 72-73 0.28255289543754714 1104 74-75 0.3093642809831678 1104 76-77 0.17844075421864858 1104 78-79 0.3058086061236409 1104 80-81 0.3542595823679804 1104 82-83 0.3613990872199295 1104 84-85 0.2995312270205872 1104 86-87 0.3381444479308655 1104 88-89 0.33649170636823555 1104 90-91 0.32613561943487923 1104 92-93 0.3032095801357215 1104 94-95 0.3093863540961408 1104 96-97 0.28119005013519427 1104 98-99 0.29908300721029946 1104 100 0.3264276698358799 1105 1 1.3384059104431607 1105 2 1.0132244998623783 1105 3 1.3338480340355368 1105 4 0.8170657082661492 1105 5 0.40981915741380703 1105 6 0.9776988951721961 1105 7 0.6954707959202722 1105 8 0.3480859432066552 1105 9 0.3434461376302096 1105 10-11 0.24761214043278557 1105 12-13 0.23955751072631415 1105 14-15 0.2959554209429385 1105 16-17 0.26964878597576813 1105 18-19 0.26021111192525126 1105 20-21 0.25839799708911215 1105 22-23 0.2712347296934823 1105 24-25 0.27236882818358055 1105 26-27 0.27379720266967666 1105 28-29 0.2744536266819111 1105 30-31 0.28292943441057616 1105 32-33 0.3116338220615873 1105 34-35 0.30951157154616027 1105 36-37 0.30500574085814947 1105 38-39 0.3143557286244061 1105 40-41 0.3296398652211394 1105 42-43 0.3104508123597327 1105 44-45 0.26849277492744505 1105 46-47 0.28368033178902863 1105 48-49 0.2713505703776917 1105 50-51 0.28856100911554705 1105 52-53 0.2860609395424234 1105 54-55 0.3171110953582428 1105 56-57 0.34706192226632027 1105 58-59 0.3118462475246062 1105 60-61 0.292721293066343 1105 62-63 0.2589043208314763 1105 64-65 0.2286749879911838 1105 66-67 0.2403829681477987 1105 68-69 0.2223853873385977 1105 70-71 0.23115044253396633 1105 72-73 0.2208122699994206 1105 74-75 0.23925945658186976 1105 76-77 0.13221718656524928 1105 78-79 0.2489275580697239 1105 80-81 0.27877354571910473 1105 82-83 0.31099544288871783 1105 84-85 0.2791508819029005 1105 86-87 0.24520220025177508 1105 88-89 0.2635429253938 1105 90-91 0.25778448692621225 1105 92-93 0.2677801890733207 1105 94-95 0.2474190044230724 1105 96-97 0.2656032386415035 1105 98-99 0.26626389769386805 1105 100 0.26090604355120917 1106 1 1.3438467005217767 1106 2 0.9884783753892599 1106 3 1.3364233841602662 1106 4 0.8182472525896358 1106 5 0.4093719812345711 1106 6 0.9586888266499258 1106 7 0.6586648716677175 1106 8 0.33574525373818176 1106 9 0.3236265056316938 1106 10-11 0.21742831016503317 1106 12-13 0.19715740758601186 1106 14-15 0.22430084825215602 1106 16-17 0.24659296997528202 1106 18-19 0.21014338624308238 1106 20-21 0.2311575174085192 1106 22-23 0.23993135319577164 1106 24-25 0.24429977148952986 1106 26-27 0.2193222524553491 1106 28-29 0.22924596630274152 1106 30-31 0.2233131255088452 1106 32-33 0.2633246113688088 1106 34-35 0.27987490019902594 1106 36-37 0.2562615985549641 1106 38-39 0.2714914851001424 1106 40-41 0.2653405551442347 1106 42-43 0.3157657587664744 1106 44-45 0.33053633689668516 1106 46-47 0.3201537396299585 1106 48-49 0.265394581451595 1106 50-51 0.2862607709507117 1106 52-53 0.26525379540990457 1106 54-55 0.2829233849352448 1106 56-57 0.2903814608749187 1106 58-59 0.2472916567009662 1106 60-61 0.24267718484842504 1106 62-63 0.22206493357624169 1106 64-65 0.14434098428356634 1106 66-67 0.14991868068502612 1106 68-69 0.16801615002558634 1106 70-71 0.17487065907307198 1106 72-73 0.12147240190957831 1106 74-75 0.1878695540262214 1106 76-77 0.04245455120902619 1106 78-79 0.19514850280325646 1106 80-81 0.22446964920005996 1106 82-83 0.22247943166217965 1106 84-85 0.21614941512594754 1106 86-87 0.21583167342778609 1106 88-89 0.22480905366627724 1106 90-91 0.2332765671432071 1106 92-93 0.21937161275309336 1106 94-95 0.1810800518217377 1106 96-97 0.20033859781929664 1106 98-99 0.21225303258036377 1106 100 0.20989527007913011 1107 1 1.2893040017747914 1107 2 0.9216534005612793 1107 3 1.2680520744844124 1107 4 0.7876008640218117 1107 5 0.38853851273168516 1107 6 0.9406507322387654 1107 7 0.6544482855848415 1107 8 0.2969562730707551 1107 9 0.2754414278849069 1107 10-11 0.17761247755002785 1107 12-13 0.1161757934465868 1107 14-15 0.1698659537251288 1107 16-17 0.19259482732469024 1107 18-19 0.15687144458625113 1107 20-21 0.13732462203421392 1107 22-23 0.17838727801994736 1107 24-25 0.1731833974062269 1107 26-27 0.13421113166443632 1107 28-29 0.16956560408520716 1107 30-31 0.16130123909388772 1107 32-33 0.17188021703124434 1107 34-35 0.18250299377408652 1107 36-37 0.17543620534122795 1107 38-39 0.20147751881341947 1107 40-41 0.19259164855886013 1107 42-43 0.20325633929322606 1107 44-45 0.17841727915768502 1107 46-47 0.19747974757909503 1107 48-49 0.19322545729721696 1107 50-51 0.1599602795828332 1107 52-53 0.15503181006360478 1107 54-55 0.18363309899321223 1107 56-57 0.19650469920075864 1107 58-59 0.19958305676912858 1107 60-61 0.15063067335238856 1107 62-63 0.15833902344558481 1107 64-65 0.11057129847412028 1107 66-67 0.11887146812650684 1107 68-69 0.11082777247733588 1107 70-71 0.13922650842921058 1107 72-73 0.093239003351826 1107 74-75 0.15732180113058547 1107 76-77 -0.0036602249101065354 1107 78-79 0.14379982612056352 1107 80-81 0.20093256949554217 1107 82-83 0.1839813685311995 1107 84-85 0.21596684127809596 1107 86-87 0.1658961384788995 1107 88-89 0.1714640273138599 1107 90-91 0.18857776678260763 1107 92-93 0.18757517380834088 1107 94-95 0.17127208857852594 1107 96-97 0.19064416127616823 1107 98-99 0.21292397795426155 1107 100 0.19072642278587182 1108 1 1.2505494749882011 1108 2 0.8803588184854796 1108 3 1.2435601349878702 1108 4 0.7420249364202078 1108 5 0.32859746263602574 1108 6 0.8723738874721363 1108 7 0.6129073852556672 1108 8 0.2686181576298097 1108 9 0.19983134852034112 1108 10-11 0.12657053492895898 1108 12-13 0.12878985804559306 1108 14-15 0.13557611659307867 1108 16-17 0.11670528280712489 1108 18-19 0.09606426307555438 1108 20-21 0.12956345765616106 1108 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-1.3184521875424124 2316 82-83 -1.403081340572328 2316 84-85 -1.093571549482519 2316 86-87 -0.8590678276938206 2316 88-89 -0.7698470200750513 2316 90-91 -0.8328449487217426 2316 92-93 -0.9057260061270256 2316 94-95 -0.8843679349784921 2316 96-97 -1.0208413065167008 2316 98-99 -1.1525121360178652 2316 100 -1.0776641248239613 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 64.0 7 755.0 8 916.0 9 339.0 10 373.0 11 727.0 12 865.0 13 1217.0 14 1998.0 15 3881.0 16 6505.0 17 10151.0 18 16464.0 19 26591.0 20 42161.0 21 64544.0 22 101930.0 23 175578.0 24 325513.0 25 490691.0 26 510205.0 27 412715.0 28 349667.0 29 356767.0 30 411701.0 31 502810.0 32 627891.0 33 811315.0 34 1125753.0 35 1767708.0 36 3299685.0 37 6607559.0 38 8087355.0 39 2240992.0 40 4344.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 15.165749807632611 29.349861312752317 29.995910370562978 25.488478509052094 2 21.49201750448991 34.893263758786944 25.701356238634304 17.913362498088837 3 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20.733776793969263 22-23 24.027503233660937 26.13055544640232 29.031699326714072 20.810241993222668 24-25 20.859333834350316 29.094484020026496 25.703233250412133 24.342948895211055 26-27 20.939736299413035 25.805559215323775 32.40848896350902 20.84621552175417 28-29 20.907218423344585 25.936788958126016 32.379037616947194 20.77695500158221 30-31 20.774082971693325 25.74982662716162 32.516848003502844 20.95924239764221 32-33 21.077743822173485 25.986696721190327 32.12413593787629 20.811423518759895 34-35 21.05009436633267 26.143526486136963 31.970559233191175 20.83581991433919 36-37 20.87054986459462 26.04659940380275 32.185809161148946 20.897041570453688 38-39 20.91413123697421 26.041608448152353 32.24584539324445 20.79841492162899 40-41 20.943105181593456 26.028034405962746 32.19575379507553 20.833106617368266 42-43 20.891879190302383 26.036783979381216 32.19043279988008 20.88090403043632 44-45 20.910779685019783 26.144537400436313 32.12701583721294 20.817667077330963 46-47 20.950221771799338 26.16947756152859 32.057535572112045 20.82276509456003 48-49 20.93616773392586 26.15046478939775 32.036936115084444 20.87643136159195 50-51 20.942637681127753 26.26031621939864 31.97242939718274 20.824616702290864 52-53 21.03719399509248 26.33688473453705 31.732229573080705 20.89369169728976 54-55 21.288565933980646 26.549617604818078 31.05562051031282 21.10619595088846 56-57 21.80373437679254 27.058270642252264 29.85820761527158 21.279787365683614 58-59 21.864548511921868 27.60304166538436 29.129941354651585 21.40246846804219 60-61 21.788048095567795 27.904541916618676 28.877981774706868 21.429428213106657 62-63 21.726137151503256 28.130138502138617 28.68973324387823 21.4539911024799 64-65 21.73350078132214 28.159535266632783 28.667829898029883 21.43913405401519 66-67 21.585622813956302 27.965887502176056 29.017162102208044 21.4313275816596 68-69 21.554554811406998 27.83553944275671 29.295836389815456 21.31406935602084 70-71 21.530624621281667 27.664228711381483 29.493303204235094 21.311843463101752 72-73 21.454584762401094 27.598270087743494 29.609474980104444 21.33767016975097 74-75 21.553425626699553 27.747763431360784 29.42290233879385 21.27590860314581 76-77 21.61561749431841 27.851130878423074 29.187753415267693 21.34549821199082 78-79 21.657119455141306 28.00340386890854 28.910143567060132 21.429333108890024 80-81 21.780362021622597 28.21942915411247 28.566251894683703 21.43395692958123 82-83 21.81588461801703 28.325827535609456 28.290409629694945 21.56787821667857 84-85 21.762141569862884 28.39191677545214 28.187497183772177 21.6584444709128 86-87 21.7942731381566 28.512608380114912 28.033450106639794 21.65966837508869 88-89 21.83208910669036 28.555137606634624 27.853510983900325 21.759262302774694 90-91 21.86382288284041 28.575967245371807 27.73310122809412 21.827108643693666 92-93 21.87498353188454 28.65506198629504 27.65456900472165 21.815385477098776 94-95 21.89426562457394 28.68975540604564 27.5081323416845 21.907846627695914 96-97 21.889196406675783 28.6559799804728 27.45090100580766 22.003922607043755 98-99 21.910352092297067 28.690478737214704 27.401900375103622 21.99726879538461 100 21.85997861323371 28.669931118322705 27.30148174906462 22.168608519378967 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 22450.0 1 17060.5 2 9690.0 3 7132.5 4 6391.5 5 6235.0 6 6357.0 7 6580.0 8 6605.5 9 6794.0 10 7727.5 11 16773.5 12 135676.0 13 159753.0 14 70434.5 15 72202.0 16 82116.5 17 91635.5 18 100185.0 19 109401.5 20 120786.5 21 136756.5 22 159653.0 23 185779.5 24 210043.5 25 238675.5 26 270826.0 27 308068.5 28 357906.5 29 409093.5 30 448897.5 31 492550.0 32 542717.5 33 579888.0 34 613707.5 35 661030.5 36 712040.0 37 747765.5 38 780156.0 39 805920.0 40 823067.0 41 857810.5 42 900441.0 43 908525.0 44 892408.0 45 895494.5 46 905079.0 47 904888.0 48 902910.5 49 902145.5 50 903606.0 51 903100.5 52 895116.5 53 871929.0 54 834500.5 55 793108.0 56 745528.5 57 689671.0 58 623129.0 59 540449.0 60 450483.0 61 368369.5 62 293646.5 63 223388.0 64 165146.0 65 120887.5 66 87324.0 67 64612.0 68 48817.0 69 36392.0 70 27421.5 71 21582.5 72 17665.5 73 14309.0 74 11892.5 75 10763.5 76 8260.5 77 4486.0 78 2372.5 79 1575.5 80 1041.5 81 721.5 82 481.5 83 292.5 84 203.0 85 157.0 86 113.0 87 66.5 88 33.0 89 21.5 90 16.5 91 9.5 92 8.0 93 7.5 94 6.0 95 5.0 96 3.0 97 0.5 98 0.0 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3318793013742205 2 0.006587353057113055 3 2.3935270625724563 4 9.616830933646333E-4 5 4.227178432372014E-5 6 0.008665715786362629 7 0.007675851503448849 8 3.099930850406144E-4 9 3.5226486936433455E-6 10-11 8.278224430061862E-5 12-13 5.283973040465018E-6 14-15 0.0 16-17 4.579443301736349E-5 18-19 0.0 20-21 3.240836798151877E-4 22-23 4.755575736418516E-5 24-25 4.790802223354949E-4 26-27 0.003487422206706912 28-29 0.006296734539887479 30-31 0.005817654317551985 32-33 0.015593004442412267 34-35 0.010115285723796866 36-37 0.005238178607447654 38-39 0.005282211716118196 40-41 0.008311689592651474 42-43 0.01921780994817127 44-45 0.03425951987002836 46-47 0.03332425664186604 48-49 0.04010007140408902 50-51 0.04022512543271336 52-53 0.06270666939554519 54-55 0.03322033850540357 56-57 0.021960191956172614 58-59 0.01641730423672481 60-61 0.017724206902066494 62-63 0.024704335288520778 64-65 0.04491377084395265 66-67 0.04774774171798872 68-69 0.050333365859122944 70-71 0.06119193045727855 72-73 0.06899459731369856 74-75 0.06001888844229532 76-77 0.059640203707728656 78-79 0.053929990175332794 80-81 0.05139720576460323 82-83 0.04813347174994267 84-85 0.050114961640117056 86-87 0.05819415641898807 88-89 0.06470224988049414 90-91 0.060822052344446 92-93 0.06522360188715336 94-95 0.07362511902149274 96-97 0.07265991327943447 98-99 0.04733383049648563 100 0.00687621024999181 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.838773E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.33116334342796 #Duplication Level Percentage of deduplicated Percentage of total 1 61.73877476438584 22.4304151058801 2 18.66156208162662 13.559925204621964 3 7.664154726982591 8.353429718259301 4 3.6138752774750467 5.251851720348879 5 2.0494720405493725 3.722985173649392 6 1.287170341955993 2.8058637566651523 7 0.8539069311574502 2.1716402537176633 8 0.6476880626607071 1.8825008640091643 9 0.47947237151478134 1.5677810143347868 >10 2.6636576267161614 17.32002383203424 >50 0.19276095681946487 4.889355361078634 >100 0.14594366802554562 9.401970658419875 >500 8.792455133334068E-4 0.19720403700668113 >1k 5.750230955050168E-4 0.3982750034863867 >5k 6.801551376329154E-5 0.1762305608061136 >10k+ 3.8866007860638745E-5 5.870547735681545 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1564237 5.510257424598585 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 38942 0.13717898542785914 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 34776 0.12250363097014098 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0020149550527639934 0.0 0.0 0.0 0.0 2 0.0020184777014576366 7.04529738728669E-6 0.0 0.0 0.0 3 0.0020466588910067834 7.04529738728669E-6 0.0 0.0 3.522648693643345E-6 4 0.0020466588910067834 2.113589216186007E-5 0.0 0.0 7.04529738728669E-6 5 0.0020466588910067834 4.5794433017363484E-5 0.0 3.522648693643345E-6 1.0567946080930036E-5 6 0.0020466588910067834 6.693032517922355E-5 0.0 3.522648693643345E-6 1.0567946080930036E-5 7 0.0020466588910067834 7.397562256651025E-5 0.0 2.113589216186007E-5 1.0567946080930036E-5 8 0.0020466588910067834 7.397562256651025E-5 0.0 2.113589216186007E-5 1.409059477457338E-5 9 0.0020466588910067834 7.397562256651025E-5 0.0 2.113589216186007E-5 1.7613243468216727E-5 10-11 0.002051942864047249 7.749827126015359E-5 0.0 2.4658540855503416E-5 2.113589216186007E-5 12-13 0.0020537041883940703 1.0567946080930035E-4 0.0 3.1703838242790106E-5 2.818118954914676E-5 14-15 0.0020537041883940703 1.092021095029437E-4 0.0 3.5226486936433454E-5 3.346516258961178E-5 16-17 0.0020625108101281786 1.092021095029437E-4 0.0 3.5226486936433454E-5 4.227178432372014E-5 18-19 0.002076601404902752 1.1800873123705207E-4 0.0 3.5226486936433454E-5 4.755575736418516E-5 20-21 0.002083646702290039 1.5675786686712885E-4 0.0 3.5226486936433454E-5 5.81237034451152E-5 22-23 0.002134725108347867 1.796550833758106E-4 0.0 3.5226486936433454E-5 7.397562256651025E-5 24-25 0.0023038122456427476 1.867003807630973E-4 0.0 3.6987811283255124E-5 7.397562256651025E-5 26-27 0.0026261346011111136 2.0959759727177902E-4 0.0 3.8749135630076795E-5 7.397562256651025E-5 28-29 0.0036441800735740404 2.3425613812728247E-4 0.0 3.8749135630076795E-5 7.749827126015359E-5 30-31 0.005726065451517258 2.4306275986139082E-4 0.0 3.8749135630076795E-5 7.749827126015359E-5 32-33 0.0097929633683285 2.5363070594232084E-4 0.0 4.2271784323720136E-5 8.454356864744029E-5 34-35 0.0172292747606096 2.730052737573592E-4 0.0 4.5794433017363484E-5 8.630489299426196E-5 36-37 0.02860038474369032 2.9766381461286267E-4 0.0 4.755575736418516E-5 8.806621734108363E-5 38-39 0.04520791200987187 3.1175440938743603E-4 0.0 5.636237909829353E-5 8.806621734108363E-5 40-41 0.0670553792078479 3.205610311215444E-4 0.0 6.340767648558021E-5 8.806621734108363E-5 42-43 0.09405824276897096 3.364129502429395E-4 0.0 6.516900083240188E-5 9.158886603472697E-5 44-45 0.12789856744445577 3.628328154452646E-4 0.0 6.693032517922355E-5 9.335019038154865E-5 46-47 0.17076920204609525 3.92775329341233E-4 0.0 8.102091995379694E-5 9.511151472837032E-5 48-49 0.22444027754244528 4.2271784323720144E-4 0.0 8.630489299426196E-5 9.6872839075192E-5 50-51 0.28588760003001296 4.420924110522398E-4 0.0 8.806621734108363E-5 9.863416342201366E-5 52-53 0.3539839219268325 4.5266035713316985E-4 0.0 9.6872839075192E-5 1.0039548776883534E-4 54-55 0.43126731161667387 4.614669788672782E-4 0.0 1.02156812115657E-4 1.02156812115657E-4 56-57 0.523486731767563 4.7379624929502995E-4 0.0 1.0391813646247868E-4 1.02156812115657E-4 58-59 0.6323277697794082 4.89648168416425E-4 0.0 1.0567946080930036E-4 1.0567946080930036E-4 60-61 0.7549194669668903 4.984547901505333E-4 0.0 1.0567946080930036E-4 1.092021095029437E-4 62-63 0.8879382042875567 5.037387631909983E-4 0.0 1.0567946080930036E-4 1.162474068902304E-4 64-65 1.0319845933436735 5.248746553528584E-4 0.0 1.0567946080930036E-4 1.162474068902304E-4 66-67 1.1945354559874988 5.319199527401451E-4 0.0 1.092021095029437E-4 1.1977005558387374E-4 68-69 1.3747946736142693 5.460105475147186E-4 0.0 1.1800873123705207E-4 1.3033800166480376E-4 70-71 1.5720260126470134 5.81237034451152E-4 0.0 1.2153137993069541E-4 1.3209932601162543E-4 72-73 1.7869833199061707 6.199861700812287E-4 0.0 1.356219747052688E-4 1.3738329905209047E-4 74-75 2.016966485168064 6.657806030985923E-4 0.0 1.4090594774573381E-4 1.4266727209255549E-4 76-77 2.2668138664134116 7.027684143818473E-4 0.0 1.4090594774573381E-4 1.532352181734855E-4 78-79 2.534496417994676 7.344722526246375E-4 0.0 1.4266727209255549E-4 1.5499654252030718E-4 80-81 2.821203033845961 7.520854960928543E-4 0.0 1.4442859643937716E-4 1.726097859885239E-4 82-83 3.126359522230203 7.785053612951793E-4 0.0 1.4442859643937716E-4 1.7789375902898893E-4 84-85 3.4522027650678657 8.137318482316127E-4 0.0 1.479512451330205E-4 1.9022302945674065E-4 86-87 3.79813919605407 8.260611186593643E-4 0.0 1.479512451330205E-4 1.93745678150384E-4 88 4.07031840869277 8.313450916998294E-4 0.0 1.5147389382666384E-4 2.0079097553767067E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 202985 0.0 85.75861 8 GTGGTAT 203030 0.0 84.95898 6 GTATCAA 205925 0.0 84.43806 9 TGGTATC 206300 0.0 84.37212 7 AGTGGTA 204655 0.0 84.31421 5 AAGCAGT 200580 0.0 83.76047 1 GCAGTGG 208615 0.0 82.72281 3 CAGTGGT 213975 0.0 80.46434 4 AGCAGTG 216930 0.0 79.25756 2 CAACGCA 199100 0.0 45.08896 12-13 ATCAACG 200945 0.0 44.757935 10-11 ACGCAGA 202610 0.0 44.402985 14-15 AGAGTAC 204635 0.0 43.876884 18-19 TCAACGC 198585 0.0 43.754616 12-13 AACGCAG 200790 0.0 43.35258 14-15 GAGTACT 202190 0.0 42.74013 20-21 CGCAGAG 205205 0.0 42.44981 16-17 AGTACTT 210045 0.0 42.434628 20-21 TATCAAC 205290 0.0 42.318714 10-11 GTACTTT 210155 0.0 41.093174 22-23 >>END_MODULE