FastQCFastQC Report
Thu 26 May 2016
SRR1660711_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660711_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24164104
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16929827.0061857042164695No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA319890.13238231386522753No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT289070.11962785791685056No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2100750.087.243768
AGTGGTA2112600.086.855035
GTATCAA2120250.086.30469
TGGTATC2120100.086.232977
GTGGTAT2131600.086.1154256
AAGCAGT2179200.084.621991
GCAGTGG2189950.084.258653
CAGTGGT2199400.083.305214
AGCAGTG2258500.081.7612842
CAACGCA2078950.045.1911812-13
ATCAACG2092650.044.999810-11
ACGCAGA2099650.044.7389114-15
AGAGTAC2087600.044.36581818-19
TCAACGC2077450.044.1908312-13
AACGCAG2089200.043.9995514-15
GAGTACT2042600.043.9479920-21
AGTACTT2107450.043.5915220-21
CGCAGAG2109300.043.51502216-17
TATCAAC2122800.043.06200410-11
GTACTTT2124550.042.23414222-23
Produced by FastQC (version 0.11.5)