##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660711_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 24164104 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.800064343374785 34.0 31.0 34.0 30.0 34.0 2 31.91562033502256 34.0 31.0 34.0 30.0 34.0 3 31.978610628393255 34.0 31.0 34.0 30.0 34.0 4 35.367327131185995 37.0 35.0 37.0 33.0 37.0 5 35.32617820217956 37.0 35.0 37.0 33.0 37.0 6 35.411299587189326 37.0 35.0 37.0 33.0 37.0 7 35.337488698111876 37.0 35.0 37.0 33.0 37.0 8 35.36608495808493 37.0 35.0 37.0 33.0 37.0 9 37.04569687334568 39.0 37.0 39.0 33.0 39.0 10-11 36.94389183641984 39.0 37.0 39.0 33.0 39.0 12-13 37.008572695267326 39.0 37.0 39.0 33.0 39.0 14-15 38.392623537789774 40.0 38.0 41.0 33.0 41.0 16-17 38.36634257160952 40.0 38.0 41.0 33.0 41.0 18-19 38.32017079962907 40.0 38.0 41.0 33.5 41.0 20-21 38.18368512650004 40.0 38.0 41.0 33.0 41.0 22-23 37.98127466261526 40.0 38.0 41.0 32.5 41.0 24-25 37.984479292093766 40.0 38.0 41.0 33.0 41.0 26-27 37.996116905472675 40.0 38.0 41.0 33.0 41.0 28-29 37.92760151586833 40.0 38.0 41.0 32.5 41.0 30-31 37.83869263267531 40.0 38.0 41.0 32.0 41.0 32-33 37.712237623211685 40.0 38.0 41.0 32.0 41.0 34-35 37.55698336673274 40.0 37.5 41.0 31.5 41.0 36-37 37.350982804907645 40.0 37.0 41.0 31.0 41.0 38-39 36.845547076771396 39.0 35.0 41.0 30.0 41.0 40-41 36.77623668562261 39.0 35.0 41.0 30.0 41.0 42-43 36.766725139074055 39.0 35.0 41.0 30.0 41.0 44-45 36.765171388105266 39.5 35.0 41.0 30.0 41.0 46-47 36.85779853455357 40.0 35.0 41.0 30.0 41.0 48-49 36.74587311410347 40.0 35.0 41.0 30.0 41.0 50-51 35.369478586915534 38.5 34.0 40.0 28.0 40.5 52-53 35.49299899553486 38.5 34.5 39.5 28.0 40.5 54-55 35.757293173378166 39.0 35.0 41.0 25.5 41.0 56-57 35.28433301313386 39.0 35.0 41.0 22.5 41.0 58-59 35.07658858362801 39.0 35.0 41.0 21.5 41.0 60-61 34.85230228275793 39.0 34.5 41.0 21.0 41.0 62-63 34.623062104847754 38.0 34.0 41.0 20.5 41.0 64-65 34.37254905044276 37.5 34.0 40.0 20.0 41.0 66-67 34.11616553214636 37.0 34.0 40.0 20.0 41.0 68-69 33.858463860278036 36.5 34.0 39.5 20.0 41.0 70-71 33.577854531663995 36.0 33.0 39.0 20.0 41.0 72-73 33.243026267392324 35.5 33.0 39.0 20.0 41.0 74-75 32.863923528884 35.0 33.0 37.5 20.0 40.0 76-77 32.46490949964459 35.0 33.0 37.0 19.0 39.5 78-79 32.08577067455098 35.0 32.0 37.0 18.0 39.0 80-81 31.76237302239719 35.0 32.0 36.0 17.5 39.0 82-83 31.44966846277437 35.0 32.0 36.0 16.0 37.0 84-85 31.167201730302104 35.0 31.5 35.5 15.0 37.0 86-87 30.956558393392115 35.0 31.0 35.0 15.0 37.0 88-89 30.7550741794523 35.0 31.0 35.0 12.5 36.0 90-91 30.58105049125761 35.0 31.0 35.0 11.5 36.0 92-93 30.422329501644256 35.0 31.0 35.0 10.5 36.0 94-95 30.23867702688252 35.0 30.5 35.0 10.0 35.5 96-97 30.103247507128756 35.0 30.0 35.0 10.0 35.0 98-99 29.996363262631213 34.0 30.0 35.0 10.0 35.0 100 29.911145019074574 34.0 30.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5144895564033725 1101 2 0.5397928885736079 1101 3 0.5445213027845739 1101 4 0.4740104330183499 1101 5 0.45000469800773146 1101 6 0.44307003747732665 1101 7 0.4769242102883098 1101 8 0.4644172480366606 1101 9 0.5566695632410372 1101 10-11 0.5907659882313752 1101 12-13 0.5720559098663571 1101 14-15 0.6897520696089572 1101 16-17 0.645642244016102 1101 18-19 0.6668900261255004 1101 20-21 0.6882610982623802 1101 22-23 0.7809400674094036 1101 24-25 0.7675120771034258 1101 26-27 0.7238339782853629 1101 28-29 0.7246542375711584 1101 30-31 0.6922368578423814 1101 32-33 0.6825256835475457 1101 34-35 0.6750861883133652 1101 36-37 0.6245111349573378 1101 38-39 0.6807116805097735 1101 40-41 0.7185799356187914 1101 42-43 0.7339598069153794 1101 44-45 0.765019872029221 1101 46-47 0.7901223444257042 1101 48-49 0.8280620330566109 1101 50-51 0.7391410540800862 1101 52-53 0.8089072181856878 1101 54-55 0.891924577700884 1101 56-57 0.9178334281185698 1101 58-59 0.818663422012122 1101 60-61 0.8297154766758723 1101 62-63 0.7943612167676477 1101 64-65 0.7278819390294018 1101 66-67 0.7286241629326256 1101 68-69 0.7146121300453601 1101 70-71 0.6830833271160515 1101 72-73 0.666912646423441 1101 74-75 0.6235646969819015 1101 76-77 0.6255393177852824 1101 78-79 0.6358988997268753 1101 80-81 0.5808611975295896 1101 82-83 0.6164968752060282 1101 84-85 0.6086115183457856 1101 86-87 0.5463406013252126 1101 88-89 0.5577494486096235 1101 90-91 0.5600402038208401 1101 92-93 0.5374959662545855 1101 94-95 0.5406126303250751 1101 96-97 0.5150249768573723 1101 98-99 0.5317049178401732 1101 100 0.5096187974454018 1102 1 0.44149681066645385 1102 2 0.4751394843247496 1102 3 0.47427687016843834 1102 4 0.4191825572884085 1102 5 0.4129558900049517 1102 6 0.3803494245338257 1102 7 0.39292302296788506 1102 8 0.44212827675772814 1102 9 0.48415657248942523 1102 10-11 0.5088987924322552 1102 12-13 0.48474952459654475 1102 14-15 0.570399189200387 1102 16-17 0.5650144601320264 1102 18-19 0.5277649753611016 1102 20-21 0.5843403665816211 1102 22-23 0.6023819063489952 1102 24-25 0.5657371471425208 1102 26-27 0.527966701911943 1102 28-29 0.5611742362644918 1102 30-31 0.5446417451008401 1102 32-33 0.5186112557342213 1102 34-35 0.5386030108129845 1102 36-37 0.5491838835545693 1102 38-39 0.5691070194082357 1102 40-41 0.5505073678378523 1102 42-43 0.568275122677079 1102 44-45 0.6078014861452132 1102 46-47 0.619752356830439 1102 48-49 0.6460121136398271 1102 50-51 0.5707192422906004 1102 52-53 0.6556452868097651 1102 54-55 0.7493188814174303 1102 56-57 0.78674875887571 1102 58-59 0.7171149787757187 1102 60-61 0.727224569955581 1102 62-63 0.6845029048900741 1102 64-65 0.6050810221201246 1102 66-67 0.6038040781082259 1102 68-69 0.5576099587031038 1102 70-71 0.5690268768724565 1102 72-73 0.5837693480001818 1102 74-75 0.5548262378268518 1102 76-77 0.5473649785162422 1102 78-79 0.5389653376550072 1102 80-81 0.5431931297328241 1102 82-83 0.5107973177207938 1102 84-85 0.5255462149539945 1102 86-87 0.47126546597406715 1102 88-89 0.4239288535261174 1102 90-91 0.45133349431454306 1102 92-93 0.4736038602962047 1102 94-95 0.522561751302792 1102 96-97 0.5085780960960982 1102 98-99 0.4482529904026116 1102 100 0.4371277436105565 1103 1 0.34340176484752405 1103 2 0.39431445223737427 1103 3 0.39207142316121235 1103 4 0.3450012717044757 1103 5 0.3521473608772254 1103 6 0.3279180922910854 1103 7 0.32660545788134243 1103 8 0.3514844830997319 1103 9 0.40245954262879735 1103 10-11 0.4109571543815278 1103 12-13 0.4354125206545163 1103 14-15 0.5045948363643475 1103 16-17 0.47312520223595556 1103 18-19 0.45798924228967763 1103 20-21 0.521936873127359 1103 22-23 0.5289333116265311 1103 24-25 0.5787838731057136 1103 26-27 0.4874899385872524 1103 28-29 0.5023077171087778 1103 30-31 0.4634487842841253 1103 32-33 0.46179635531130003 1103 34-35 0.4734405060012179 1103 36-37 0.46446382919792484 1103 38-39 0.5118922672580553 1103 40-41 0.4704009288032722 1103 42-43 0.5180014195681935 1103 44-45 0.5426688430462221 1103 46-47 0.5930877430100665 1103 48-49 0.6352243702716436 1103 50-51 0.5551243503619929 1103 52-53 0.5694196124938102 1103 54-55 0.7027074216489879 1103 56-57 0.7901318682457301 1103 58-59 0.7093467600872501 1103 60-61 0.678710793922761 1103 62-63 0.6372508507325776 1103 64-65 0.6025218690681129 1103 66-67 0.5601248182850185 1103 68-69 0.5536211383090759 1103 70-71 0.5380174610520072 1103 72-73 0.548627108313454 1103 74-75 0.5579154418682108 1103 76-77 0.548303596740567 1103 78-79 0.5600983704008868 1103 80-81 0.4706413728441383 1103 82-83 0.44850009372510513 1103 84-85 0.4762795324298885 1103 86-87 0.4493928240070524 1103 88-89 0.37640178695255244 1103 90-91 0.373265162385227 1103 92-93 0.39561609931942243 1103 94-95 0.46041992931024467 1103 96-97 0.4325234812604606 1103 98-99 0.43868763585084025 1103 100 0.42079922618158605 1104 1 0.37557134621647137 1104 2 0.40905000008621073 1104 3 0.3818527587818821 1104 4 0.33702321527814405 1104 5 0.3682519137368061 1104 6 0.2876063096534125 1104 7 0.31860944425608295 1104 8 0.3353023008372489 1104 9 0.38249227903439476 1104 10-11 0.36142893195909664 1104 12-13 0.3689640812646928 1104 14-15 0.4400411128681867 1104 16-17 0.425111647944739 1104 18-19 0.4283159738326461 1104 20-21 0.3845344035496936 1104 22-23 0.4625344705138019 1104 24-25 0.4847671531639648 1104 26-27 0.41605501337805606 1104 28-29 0.41606033972374235 1104 30-31 0.40060278423475637 1104 32-33 0.35619903161273214 1104 34-35 0.3650630870245877 1104 36-37 0.37638615968443645 1104 38-39 0.3763859945801471 1104 40-41 0.37204191441283285 1104 42-43 0.36190218907428573 1104 44-45 0.4053916727552078 1104 46-47 0.44116010558504826 1104 48-49 0.4958843898949752 1104 50-51 0.4307846785282621 1104 52-53 0.4499184801412426 1104 54-55 0.5667303587309149 1104 56-57 0.6208605232453479 1104 58-59 0.5733336284998529 1104 60-61 0.5860580343147319 1104 62-63 0.5224173559284466 1104 64-65 0.5023190637957384 1104 66-67 0.5035910822003018 1104 68-69 0.47864687079000845 1104 70-71 0.45073572432552567 1104 72-73 0.43494961590361214 1104 74-75 0.4287222353665996 1104 76-77 0.4121245924703345 1104 78-79 0.4130533323884009 1104 80-81 0.372461745317171 1104 82-83 0.4066217614613876 1104 84-85 0.415822672903321 1104 86-87 0.38350202969411384 1104 88-89 0.3591447182338676 1104 90-91 0.3514392669936228 1104 92-93 0.36846976666077325 1104 94-95 0.4678700224902137 1104 96-97 0.4014566014934715 1104 98-99 0.3799117645255876 1104 100 0.3654488448225166 1105 1 0.292696937832428 1105 2 0.3235150102281992 1105 3 0.32244014891034567 1105 4 0.26719224840863376 1105 5 0.286765267354113 1105 6 0.2493989060022983 1105 7 0.28026596893491984 1105 8 0.26628860915367625 1105 9 0.3003852810627947 1105 10-11 0.29586393872042294 1105 12-13 0.3088952002640184 1105 14-15 0.36809300603925266 1105 16-17 0.33881643158233743 1105 18-19 0.34932890486712154 1105 20-21 0.3539199681203513 1105 22-23 0.41734261791069116 1105 24-25 0.43798174072312435 1105 26-27 0.39197920201903 1105 28-29 0.35979005574807843 1105 30-31 0.3843857456952051 1105 32-33 0.37589096873240635 1105 34-35 0.4036715217778024 1105 36-37 0.42229767084062075 1105 38-39 0.42678956117919853 1105 40-41 0.4106364039327772 1105 42-43 0.42401806128523845 1105 44-45 0.4614591169742752 1105 46-47 0.4574265863152718 1105 48-49 0.4786882269470354 1105 50-51 0.41026870489743317 1105 52-53 0.4209702887957363 1105 54-55 0.5050273499411944 1105 56-57 0.5123336469776021 1105 58-59 0.4782060084863389 1105 60-61 0.4411155055791056 1105 62-63 0.4401899218513634 1105 64-65 0.43597694073961435 1105 66-67 0.39551383405967044 1105 68-69 0.36133196206790075 1105 70-71 0.38482379058650906 1105 72-73 0.393400427262641 1105 74-75 0.37056680940310827 1105 76-77 0.3545290887516046 1105 78-79 0.3984070342139603 1105 80-81 0.326888723004803 1105 82-83 0.368471406491814 1105 84-85 0.34901063774442775 1105 86-87 0.3012259985920167 1105 88-89 0.3290192111344794 1105 90-91 0.2848300715913261 1105 92-93 0.253400885660092 1105 94-95 0.3171588155936611 1105 96-97 0.3226195494312698 1105 98-99 0.3185810512057792 1105 100 0.2910522930983781 1106 1 0.23119913161974992 1106 2 0.2830656047518829 1106 3 0.24261597351814146 1106 4 0.2168781251343006 1106 5 0.24338135934722516 1106 6 0.19793994935144354 1106 7 0.19893245848602703 1106 8 0.21019172833631927 1106 9 0.2060556125196058 1106 10-11 0.20358390681393246 1106 12-13 0.21234668746242136 1106 14-15 0.3029809894490114 1106 16-17 0.23790962202215837 1106 18-19 0.2184757313939869 1106 20-21 0.2713791058372479 1106 22-23 0.26441500394433604 1106 24-25 0.23943948117020852 1106 26-27 0.28908644162606834 1106 28-29 0.27397590053487164 1106 30-31 0.27770743851014856 1106 32-33 0.282286871307349 1106 34-35 0.30172945408984475 1106 36-37 0.2601978318946223 1106 38-39 0.2835419625691884 1106 40-41 0.3002403938852112 1106 42-43 0.3349961171443141 1106 44-45 0.35558261437457617 1106 46-47 0.35335402625106127 1106 48-49 0.3375627137264914 1106 50-51 0.255716047259412 1106 52-53 0.3189378241885592 1106 54-55 0.3971430362491972 1106 56-57 0.41240688303474116 1106 58-59 0.35796226038095824 1106 60-61 0.34007359270533044 1106 62-63 0.34268845842843376 1106 64-65 0.29774662764820903 1106 66-67 0.33128855216422437 1106 68-69 0.30226031661684516 1106 70-71 0.3190600719894121 1106 72-73 0.28680460905490435 1106 74-75 0.29402943954075056 1106 76-77 0.2904093286739595 1106 78-79 0.287605298936505 1106 80-81 0.24339782235875518 1106 82-83 0.2864127245096775 1106 84-85 0.309445832935328 1106 86-87 0.21061047852527537 1106 88-89 0.2592593540419479 1106 90-91 0.22907166708930404 1106 92-93 0.22198168676294472 1106 94-95 0.30206958301942777 1106 96-97 0.30303426102855724 1106 98-99 0.24336613979789945 1106 100 0.2603722773790942 1107 1 0.21177431077220987 1107 2 0.2471634829815983 1107 3 0.23827832231616952 1107 4 0.23427626797137435 1107 5 0.25747062078186644 1107 6 0.22968452580854404 1107 7 0.2286486778743253 1107 8 0.24662892381737578 1107 9 0.24757095867118295 1107 10-11 0.21107732914835964 1107 12-13 0.23738112478361728 1107 14-15 0.2804139151293725 1107 16-17 0.25827608360230414 1107 18-19 0.2527538944025238 1107 20-21 0.24543831364945135 1107 22-23 0.3047603152453746 1107 24-25 0.27770250040912003 1107 26-27 0.26983306835070664 1107 28-29 0.27519106814283134 1107 30-31 0.24461769231896113 1107 32-33 0.20219209026699758 1107 34-35 0.27515230292554094 1107 36-37 0.2572494831932488 1107 38-39 0.3213066008984171 1107 40-41 0.2962033735304317 1107 42-43 0.35003414993322224 1107 44-45 0.3393231386005482 1107 46-47 0.32762758364392397 1107 48-49 0.374512055087159 1107 50-51 0.2758597292280456 1107 52-53 0.3081248398209553 1107 54-55 0.44103059986570514 1107 56-57 0.4335415832852263 1107 58-59 0.32216494328195466 1107 60-61 0.3567604922180294 1107 62-63 0.32639158762644627 1107 64-65 0.2962479704763652 1107 66-67 0.3154269478748972 1107 68-69 0.3076455794651949 1107 70-71 0.293457254395193 1107 72-73 0.28835546178179783 1107 74-75 0.3166405266941652 1107 76-77 0.2612278711713145 1107 78-79 0.29669405415573635 1107 80-81 0.2504209875971988 1107 82-83 0.29211496793244507 1107 84-85 0.25795429133151515 1107 86-87 0.20670370016211237 1107 88-89 0.24629990532573132 1107 90-91 0.28119985385395196 1107 92-93 0.28084066094550764 1107 94-95 0.28475758001097873 1107 96-97 0.32774328112749274 1107 98-99 0.3149416931107325 1107 100 0.3177765691759582 1108 1 0.14671588587493645 1108 2 0.17527301904520698 1108 3 0.2036452703077316 1108 4 0.17870812028148464 1108 5 0.18005385030799914 1108 6 0.1344122999352635 1108 7 0.13668069813668637 1108 8 0.18789659330676045 1108 9 0.20192887543674232 1108 10-11 0.17410309614177066 1108 12-13 0.1851000185525109 1108 14-15 0.2306143734364099 1108 16-17 0.17570548378358097 1108 18-19 0.1658929934948148 1108 20-21 0.18080131585912795 1108 22-23 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-1.4315495841143715 2316 90-91 -1.3337337053845566 2316 92-93 -1.2204981088175977 2316 94-95 -1.38264328569419 2316 96-97 -1.6115264695988678 2316 98-99 -1.459380955472934 2316 100 -1.287591025552146 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12090.0 3 3193.0 4 2256.0 5 1896.0 6 3740.0 7 21829.0 8 40586.0 9 44916.0 10 45446.0 11 42500.0 12 40869.0 13 41846.0 14 46900.0 15 54739.0 16 66525.0 17 80641.0 18 89783.0 19 99691.0 20 114620.0 21 140587.0 22 184146.0 23 271389.0 24 407637.0 25 515037.0 26 509957.0 27 426617.0 28 364330.0 29 343779.0 30 373825.0 31 447753.0 32 553578.0 33 712929.0 34 982948.0 35 1505275.0 36 2636967.0 37 4929492.0 38 6175736.0 39 1824256.0 40 3800.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 16.521248221355666 34.7230101798937 27.398537957785745 21.35720364096489 2 20.73046382602986 34.95487419438383 25.73415586564017 18.58050611394614 3 27.739625799523708 24.810107418976457 26.096027743020883 21.35423903847895 4 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20.22566396260843 24-25 20.28917755114924 29.93571125953474 25.308507583639066 24.466603605676948 26-27 20.320905263484022 25.8772859955742 33.54811348490422 20.253695256037552 28-29 20.290948398616685 25.99083036063617 33.53625965621723 20.18196158452992 30-31 20.265777943924117 25.932657209415893 33.52279153028154 20.278773316378455 32-33 20.35164486543511 26.057221451812822 33.33627600747942 20.25485767527265 34-35 20.369135846657905 26.170769676650895 33.24220228424999 20.2178921924412 36-37 20.304569831654963 26.110973752165783 33.31416590915573 20.27029050702352 38-39 20.334366456502966 26.113736368049622 33.31005530182575 20.241841873621663 40-41 20.340532113169 26.154095215792616 33.27381408183085 20.23155858920753 42-43 20.347841026712146 26.1783099406671 33.245945905208146 20.22790312741261 44-45 20.379667620997182 26.24288274099813 33.18487632180477 20.192573316199912 46-47 20.354064807714252 26.359765630096398 33.13024809387553 20.15592146831382 48-49 20.410013603402167 26.368543988631004 33.01259596135213 20.208846446614697 50-51 20.427248808726812 26.435228319136588 32.93873911689356 20.198783755243042 52-53 20.53913788577966 26.57825033571994 32.64992397418171 20.23268780431869 54-55 20.93615126008436 26.91037426803145 31.709495406570987 20.443979065313204 56-57 21.492622030541444 27.563261443513724 30.291231177563972 20.652885348380856 58-59 21.60792034154206 28.186006584901108 29.517813802391696 20.688259271165137 60-61 21.74177094454278 28.507278838030604 29.069059717554396 20.681890499872218 62-63 21.78423910373138 28.715258740782456 28.85472589171024 20.645776263775925 64-65 21.85576508383061 28.760414102442756 28.81342990113834 20.570390912588294 66-67 21.893430589791304 28.521736644329003 29.08369185050383 20.50114091537586 68-69 21.948124253301383 28.284450403300127 29.41816638997657 20.349258953421916 70-71 22.01405108556351 28.108158705469794 29.64984827704115 20.227941931925542 72-73 22.140865035509186 27.999040291694318 29.68771508529167 20.172379587504825 74-75 22.33938520145792 28.102735717829447 29.49508175666788 20.062797324044755 76-77 22.62524788563112 28.25935139674607 29.108751074870217 20.006649642752606 78-79 22.908796550613094 28.444509526834132 28.66109882173485 19.98559510081793 80-81 23.191790965506648 28.684583508089958 28.204270701057553 19.919354825345845 82-83 23.388153450645966 28.878763105429115 27.819892780607 19.913190663317923 84-85 23.525203785050646 28.960455110279092 27.60020667697041 19.91413442769985 86-87 23.719519133320176 28.99368481053644 27.42593252057376 19.86086353556962 88-89 23.954404105569903 29.017786990409068 27.23019279668743 19.797616107333603 90-91 24.17945981264493 28.996928625463074 27.070276790066007 19.75333477182599 92-93 24.39408146786241 28.998949172890153 26.925610839880903 19.681358519366533 94-95 24.603781189329606 28.998493738441404 26.788013773120767 19.609711299108223 96-97 24.758479414079467 28.952200340871766 26.679099661295407 19.61022058375336 98-99 24.944227589431275 28.96164772877358 26.56972964782225 19.524395033972894 100 25.054798200135544 28.950818369370136 26.453927188115138 19.540456242379182 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 35257.0 1 25022.0 2 12732.0 3 10136.0 4 9430.0 5 9505.5 6 9833.5 7 9753.0 8 9550.5 9 9881.0 10 10705.0 11 14042.0 12 131440.0 13 163348.0 14 78101.0 15 80400.5 16 90216.0 17 99129.0 18 106721.0 19 114310.5 20 123213.5 21 135968.0 22 153957.0 23 175251.5 24 197156.0 25 220841.0 26 247042.0 27 280044.0 28 321533.0 29 362558.5 30 396575.5 31 430587.5 32 466139.5 33 494817.0 34 520058.0 35 550115.0 36 584057.5 37 616199.0 38 650711.5 39 678161.0 40 692678.0 41 716155.0 42 749628.5 43 760672.0 44 757511.5 45 768300.5 46 780890.0 47 784539.5 48 782746.0 49 778564.0 50 771175.5 51 762886.5 52 752886.0 53 725498.5 54 684064.0 55 642490.5 56 598621.5 57 547918.5 58 490159.5 59 424337.0 60 352616.0 61 286310.5 62 226599.5 63 172353.0 64 128577.5 65 93943.0 66 68142.5 67 51298.0 68 39111.5 69 29879.0 70 23045.0 71 18138.0 72 15102.0 73 12627.0 74 10616.0 75 9494.0 76 7792.5 77 5578.0 78 4028.5 79 3171.5 80 2670.0 81 2259.5 82 1874.5 83 1580.0 84 1336.0 85 1170.5 86 1010.5 87 818.5 88 698.0 89 598.0 90 548.5 91 490.0 92 408.5 93 360.5 94 319.0 95 272.0 96 209.5 97 158.0 98 122.5 99 96.5 100 62.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40856056570522953 2 0.38651546939211984 3 0.16964005783123595 4 0.1757027696950816 5 0.277763247501335 6 0.10141075373620309 7 0.13594545032582214 8 0.14193367153195502 9 0.13479084513127404 10-11 0.1309318152247648 12-13 0.13277338981821962 14-15 0.1386436674829739 16-17 0.1431772516787711 18-19 0.15443568691808313 20-21 0.13493154970695376 22-23 0.09714823276708294 24-25 0.09268293167418912 26-27 0.08741064845607352 28-29 0.09753723953513857 30-31 0.10239154739608801 32-33 0.10371789494036276 34-35 0.10445245559280825 36-37 0.10010095967142006 38-39 0.0994574431561791 40-41 0.1005437652478238 42-43 0.06182724590160678 44-45 0.0645047712093939 46-47 0.08367370046081576 48-49 0.08792587550525358 50-51 0.09787451667978254 52-53 0.10319439115143686 54-55 0.10594640711693677 56-57 0.10686512522872771 58-59 0.10635817491929352 60-61 0.10410069415360901 62-63 0.10826803261565171 64-65 0.11547086537949017 66-67 0.12404349857126919 68-69 0.12915024699446748 70-71 0.1274969682302311 72-73 0.11423349278748346 74-75 0.1114421623081907 76-77 0.11367688203957407 78-79 0.11246847803667787 80-81 0.10665406836520817 82-83 0.10963783304359226 84-85 0.11541085901633265 86-87 0.12902609589827954 88-89 0.13649999188879505 90-91 0.14351659800835156 92-93 0.14293929541107753 94-95 0.13314377392184704 96-97 0.12942751777595396 98-99 0.12449665007235526 100 0.11889950481921449 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.4164104E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.173631293760955 #Duplication Level Percentage of deduplicated Percentage of total 1 69.46691253443531 29.296719563432163 2 15.825426699096488 13.348314213482714 3 5.759316259624131 7.286738413126516 4 2.6375761167738405 4.449446506321989 5 1.5481891766655715 3.2646379754842583 6 0.9870784321288777 2.497720911277616 7 0.6560426452117554 1.936739044250094 8 0.4878541393038827 1.6459664476909635 9 0.38322552786142017 1.4545810902946006 >10 1.9737693975892576 14.990632954834648 >50 0.17234347617749557 5.182809382985605 >100 0.1011304992606172 6.6401185869288755 >500 4.7201111191029596E-4 0.1355200336278932 >1k 5.948753971897815E-4 0.5451726278094442 >5k 2.923258384547095E-5 0.07575827688032681 >10k+ 3.897677846029794E-5 7.2491239715721525 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1692982 7.0061857042164695 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 31989 0.13238231386522753 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 28907 0.11962785791685056 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0010139006188683844 8.276739745864362E-6 0.0 0.0 4.138369872932181E-6 2 0.0010180389887413164 1.6553479491728724E-5 0.0 0.0 1.2415109618796542E-5 3 0.0010428692079789096 1.6553479491728724E-5 0.0 0.0 1.6553479491728724E-5 4 0.0010470075778518417 3.310695898345745E-5 0.0 0.0 2.4830219237593084E-5 5 0.001051145947724774 4.966043847518617E-5 0.0 0.0 3.310695898345745E-5 6 0.001051145947724774 5.379880834811835E-5 0.0 0.0 3.310695898345745E-5 7 0.0010635610573435704 5.793717822105053E-5 0.0 0.0 3.310695898345745E-5 8 0.001075976166962367 6.62139179669149E-5 0.0 0.0 3.724532885638963E-5 9 0.001075976166962367 7.449065771277926E-5 0.0 4.138369872932181E-6 3.724532885638963E-5 10-11 0.0010883912765811635 9.311332214097407E-5 0.0 4.138369872932181E-6 4.7591253538720074E-5 12-13 0.0011070139410093583 1.0345924682330452E-4 0.0 8.276739745864362E-6 6.207554809398272E-5 14-15 0.0011132214958187565 1.1587435644210106E-4 0.0 8.276739745864362E-6 6.828310290338098E-5 16-17 0.0011380517150563498 1.262202811244315E-4 0.0 8.276739745864362E-6 8.069821252217751E-5 18-19 0.0011483976397386802 1.6346560998082112E-4 0.0 1.0345924682330451E-5 9.932087695037234E-5 20-21 0.0011897813384680019 1.8415745934548207E-4 0.0 2.0691849364660902E-5 1.0759761669623669E-4 22-23 0.0012766871057995777 1.9657256896427858E-4 0.0 2.0691849364660902E-5 1.2208191125149932E-4 24-25 0.0015912032161424234 2.0484930871014296E-4 0.0 2.0691849364660902E-5 1.2415109618796541E-4 26-27 0.002543028286916825 2.338178978206682E-4 0.0 2.2761034301126993E-5 1.262202811244315E-4 28-29 0.00545644067746108 2.586481170582613E-4 0.0 2.8968589110525266E-5 1.3656620580676195E-4 30-31 0.012566160119158568 2.8968589110525266E-4 0.0 3.310695898345745E-5 1.6139642504435506E-4 32-33 0.026738007749014817 2.9382426097818485E-4 0.0 3.310695898345745E-5 1.738115346631516E-4 34-35 0.05189722739150601 3.0830855553344745E-4 0.0 4.5522068602253986E-5 1.7794990453608378E-4 36-37 0.09252981198889063 3.290004048981084E-4 4.138369872932181E-6 4.7591253538720074E-5 1.9450338402781248E-4 38-39 0.1504587134701953 3.372771446439727E-4 8.276739745864362E-6 5.793717822105053E-5 2.1933360326540556E-4 40-41 0.2272772042364989 3.455538843898371E-4 8.276739745864362E-6 5.793717822105053E-5 2.2347197313833775E-4 42-43 0.3228921709656605 3.7245328856389627E-4 8.276739745864362E-6 6.207554809398272E-5 2.2347197313833775E-4 44-45 0.44388982931045157 3.8900676805562497E-4 8.276739745864362E-6 6.62139179669149E-5 2.2761034301126994E-4 46-47 0.5948099710214787 4.1383698729321805E-4 8.276739745864362E-6 6.62139179669149E-5 2.3795626769360038E-4 48-49 0.77742795677423 4.4073639146727727E-4 8.276739745864362E-6 6.62139179669149E-5 2.565789321217952E-4 50-51 0.9894759598783386 4.5728987095900597E-4 8.276739745864362E-6 6.62139179669149E-5 2.586481170582613E-4 52-53 1.227920555216945 4.7798172032366686E-4 8.276739745864362E-6 7.655984264924535E-5 2.731324116135239E-4 54-55 1.4985927059410107 5.090194943706582E-4 8.276739745864362E-6 8.069821252217751E-5 2.793399664229222E-4 56-57 1.8085400559441394 5.400572684176496E-4 8.276739745864362E-6 8.69057673315758E-5 2.9382426097818485E-4 58-59 2.159246210825777 5.793717822105053E-4 8.276739745864362E-6 9.518250707744015E-5 3.0417018566051526E-4 60-61 2.5477274059075397 6.124787411939627E-4 8.276739745864362E-6 9.932087695037234E-5 3.331387747710406E-4 62-63 2.9641653586658956 6.290322206856915E-4 8.276739745864362E-6 1.0345924682330451E-4 3.5589980907216757E-4 64-65 3.4143620636626957 6.517932549868185E-4 8.276739745864362E-6 1.0966680163270278E-4 3.641765488180319E-4 66-67 3.902764199326406 6.766234742244116E-4 8.276739745864362E-6 1.282894660608976E-4 3.683149186909641E-4 68-69 4.42880687816937 6.952461386526064E-4 8.276739745864362E-6 1.4070457567969414E-4 3.7245328856389627E-4 70-71 4.987592339446975 7.055920633349368E-4 8.276739745864362E-6 1.4898131542555852E-4 3.7659165843682846E-4 72-73 5.577086574366672 7.32491467508996E-4 8.276739745864362E-6 1.4898131542555852E-4 3.848683981826928E-4 74-75 6.197651690292345 7.573216867465891E-4 8.276739745864362E-6 1.5311968529849068E-4 3.9521432286502325E-4 76-77 6.846523256148873 7.738751662383179E-4 8.276739745864362E-6 1.5311968529849068E-4 4.0142187767442153E-4 78-79 7.528832850578693 7.904286457300465E-4 8.276739745864362E-6 1.5725805517142287E-4 4.055602475473537E-4 80-81 8.239101271870044 8.049129402853091E-4 8.276739745864362E-6 1.6139642504435506E-4 4.096986174202859E-4 82-83 8.975337964114043 8.359507143323005E-4 8.276739745864362E-6 1.6553479491728722E-4 4.1383698729321805E-4 84-85 9.736582825500172 8.545733787604954E-4 8.276739745864362E-6 1.758807195996177E-4 4.221137270390824E-4 86-87 10.521774364156023 8.628501185063597E-4 8.276739745864362E-6 1.8208827440901595E-4 4.34528836657879E-4 88 11.12515076081447 8.897495226804188E-4 8.276739745864362E-6 1.8208827440901595E-4 4.428055764037433E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 210075 0.0 87.24376 8 AGTGGTA 211260 0.0 86.85503 5 GTATCAA 212025 0.0 86.3046 9 TGGTATC 212010 0.0 86.23297 7 GTGGTAT 213160 0.0 86.115425 6 AAGCAGT 217920 0.0 84.62199 1 GCAGTGG 218995 0.0 84.25865 3 CAGTGGT 219940 0.0 83.30521 4 AGCAGTG 225850 0.0 81.761284 2 CAACGCA 207895 0.0 45.19118 12-13 ATCAACG 209265 0.0 44.9998 10-11 ACGCAGA 209965 0.0 44.73891 14-15 AGAGTAC 208760 0.0 44.365818 18-19 TCAACGC 207745 0.0 44.19083 12-13 AACGCAG 208920 0.0 43.99955 14-15 GAGTACT 204260 0.0 43.94799 20-21 AGTACTT 210745 0.0 43.59152 20-21 CGCAGAG 210930 0.0 43.515022 16-17 TATCAAC 212280 0.0 43.062004 10-11 GTACTTT 212455 0.0 42.234142 22-23 >>END_MODULE