FastQCFastQC Report
Thu 26 May 2016
SRR1660711_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660711_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24164104
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14783836.118095667855096No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT302260.1250863677792481No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT290580.1202527517676633No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA281650.11655718747113487No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1917450.086.5702748
AGTGGTA1919750.085.74865
TGGTATC1936550.085.6139147
AAGCAGT1877950.085.465441
GTATCAA1940600.085.428539
GTGGTAT1936650.085.017116
GCAGTGG1941800.084.7398453
AGCAGTG2001650.081.967132
CAGTGGT2012900.081.6040344
CAACGCA1886800.044.93834712-13
ATCAACG1903600.044.6318410-11
ACGCAGA1907600.044.53210414-15
AGAGTAC1925750.043.98718318-19
TCAACGC1887400.043.8981712-13
AACGCAG1898150.043.7213614-15
GAGTACT1878450.043.6954820-21
CGCAGAG1923300.043.1570516-17
AGTACTT1945200.043.14565720-21
TATCAAC1938500.042.7701310-11
GTACTTT1962400.041.7672122-23
Produced by FastQC (version 0.11.5)