##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660711_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 24164104 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.33018911026041 34.0 31.0 34.0 27.0 34.0 2 31.95642275831953 34.0 31.0 34.0 30.0 34.0 3 31.683838391028278 34.0 31.0 34.0 30.0 34.0 4 35.597922356235514 37.0 35.0 37.0 33.0 37.0 5 35.9242561197386 37.0 35.0 37.0 35.0 37.0 6 35.37602056339436 37.0 35.0 37.0 33.0 37.0 7 35.57830685549111 37.0 35.0 37.0 35.0 37.0 8 35.961146252308794 37.0 35.0 37.0 35.0 37.0 9 37.77072797733365 39.0 38.0 39.0 35.0 39.0 10-11 37.76966033998198 39.0 37.5 39.0 35.0 39.0 12-13 37.862006656650706 39.0 38.0 39.0 35.0 39.0 14-15 39.33001142934991 41.0 39.0 41.0 36.0 41.0 16-17 39.29246143370348 41.0 39.0 41.0 36.0 41.0 18-19 39.28086470741891 41.0 39.0 41.0 36.0 41.0 20-21 39.20903804254443 40.5 39.0 41.0 36.0 41.0 22-23 39.06680138026223 40.0 38.5 41.0 35.5 41.0 24-25 39.08552632450183 40.0 39.0 41.0 36.0 41.0 26-27 39.055132356655974 40.0 39.0 41.0 35.5 41.0 28-29 38.981741077591785 40.0 39.0 41.0 35.0 41.0 30-31 38.88784369989469 40.0 38.0 41.0 35.0 41.0 32-33 38.76442375020402 40.0 38.0 41.0 35.0 41.0 34-35 38.676326587569726 40.0 38.0 41.0 35.0 41.0 36-37 38.538479101066606 40.0 38.0 41.0 34.5 41.0 38-39 38.09637626125099 40.0 37.0 41.0 34.0 41.0 40-41 37.961416322326706 40.0 37.0 41.0 33.0 41.0 42-43 37.79533751799777 40.0 36.0 41.0 33.0 41.0 44-45 37.66624071805021 40.0 36.0 41.0 33.0 41.0 46-47 37.50993784002916 40.0 36.0 41.0 33.0 41.0 48-49 37.54468094492558 40.0 35.5 41.0 33.0 41.0 50-51 37.52789205426363 40.0 36.0 41.0 33.0 41.0 52-53 37.24851453213411 40.0 35.0 41.0 32.5 41.0 54-55 36.668014899290284 39.5 35.0 41.0 30.5 41.0 56-57 36.11835642654079 39.0 35.0 41.0 27.5 41.0 58-59 35.96231627293112 39.0 35.0 41.0 27.5 41.0 60-61 35.810712989813325 39.0 35.0 41.0 27.0 41.0 62-63 35.56637570753709 38.5 35.0 41.0 27.0 41.0 64-65 35.297303905826595 38.0 35.0 40.0 26.5 41.0 66-67 35.02110959711148 37.0 35.0 40.0 26.0 41.0 68-69 34.75992445240262 37.0 34.5 40.0 26.0 41.0 70-71 34.46746072190386 36.0 34.0 39.0 26.0 41.0 72-73 34.13088707944644 36.0 34.0 39.0 26.0 41.0 74-75 33.74977619695727 35.0 34.0 38.5 25.5 40.0 76-77 32.05077920538663 34.5 31.5 36.0 23.0 39.0 78-79 32.97577427244974 35.0 33.5 37.0 25.0 39.0 80-81 32.87166602577112 35.0 34.0 36.5 24.5 39.0 82-83 32.616320348563306 35.0 34.0 36.0 24.0 37.5 84-85 32.37050248169764 35.0 33.5 36.0 24.0 37.0 86-87 32.15457223243204 35.0 33.0 35.0 23.0 37.0 88-89 31.98129672012668 35.0 33.0 35.0 23.0 36.0 90-91 31.818455962612973 35.0 33.0 35.0 21.0 36.0 92-93 31.67137684889951 35.0 33.0 35.0 20.0 36.0 94-95 31.560644520483773 35.0 33.0 35.0 20.0 36.0 96-97 31.457894072960457 35.0 33.0 35.0 19.5 35.0 98-99 31.363002058756244 35.0 33.0 35.0 19.0 35.0 100 31.30067545645392 35.0 33.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.5861083240107021 1101 2 1.1919996889143576 1101 3 1.5646524268076512 1101 4 0.9588083780587269 1101 5 0.5788151206062082 1101 6 1.1276063043256173 1101 7 0.8311110836402023 1101 8 0.4868356157818283 1101 9 0.5356786552310808 1101 10-11 0.4420829477604329 1101 12-13 0.42334217508920347 1101 14-15 0.5506622509131276 1101 16-17 0.5841150551510665 1101 18-19 0.5156850103658073 1101 20-21 0.5258373077232221 1101 22-23 0.5185896666816632 1101 24-25 0.5233225198033225 1101 26-27 0.5324540767595849 1101 28-29 0.5159306178010041 1101 30-31 0.5225132209226189 1101 32-33 0.5864884206841481 1101 34-35 0.573208459435719 1101 36-37 0.5675995971184378 1101 38-39 0.5691049631562777 1101 40-41 0.5830291243490038 1101 42-43 0.5828539486513122 1101 44-45 0.5641671417853615 1101 46-47 0.5938426638189043 1101 48-49 0.5696809642872012 1101 50-51 0.5092004319331096 1101 52-53 0.487463202390245 1101 54-55 0.5251222029692428 1101 56-57 0.571088848319441 1101 58-59 0.5559373988449821 1101 60-61 0.58749851640966 1101 62-63 0.5296954153751585 1101 64-65 0.48504651769880525 1101 66-67 0.4794511454790751 1101 68-69 0.4613321199273557 1101 70-71 0.45985537358115636 1101 72-73 0.4476932480657183 1101 74-75 0.4582552783605891 1101 76-77 0.38237612613642114 1101 78-79 0.49649361938354275 1101 80-81 0.5061914601618724 1101 82-83 0.4667622560744391 1101 84-85 0.47320424062697697 1101 86-87 0.4898501400341857 1101 88-89 0.44205596782920864 1101 90-91 0.4345825958707188 1101 92-93 0.4477972814951947 1101 94-95 0.4034960689805658 1101 96-97 0.43280369993762235 1101 98-99 0.4763057030390101 1101 100 0.45665270153021 1102 1 1.5207152088390785 1102 2 1.1452144070691972 1102 3 1.5037857547198605 1102 4 0.9080901157696175 1102 5 0.4729006186798017 1102 6 1.050081147117666 1102 7 0.7865218057060162 1102 8 0.44952704941279364 1102 9 0.44569343568468156 1102 10-11 0.3731192672050341 1102 12-13 0.34793172558480023 1102 14-15 0.46539525312552854 1102 16-17 0.47085789114654375 1102 18-19 0.3990182971649645 1102 20-21 0.4018899578606039 1102 22-23 0.42359254012683323 1102 24-25 0.42866117419085725 1102 26-27 0.39148230083056745 1102 28-29 0.4256473338718365 1102 30-31 0.44756498815989687 1102 32-33 0.45083079770827084 1102 34-35 0.4059408184431206 1102 36-37 0.4222535600378663 1102 38-39 0.46178693386484326 1102 40-41 0.4962917050611324 1102 42-43 0.4939107033412782 1102 44-45 0.45677605565695956 1102 46-47 0.49715915089993246 1102 48-49 0.46154081408506187 1102 50-51 0.4598314734840585 1102 52-53 0.4646318503971827 1102 54-55 0.504204370404878 1102 56-57 0.5835345400668288 1102 58-59 0.5686799011578998 1102 60-61 0.6145519161408615 1102 62-63 0.6033707511607673 1102 64-65 0.5167970837435547 1102 66-67 0.4978239939140252 1102 68-69 0.4417706939963324 1102 70-71 0.43735699745359824 1102 72-73 0.43948838526178235 1102 74-75 0.44633083480761826 1102 76-77 0.2879470406697635 1102 78-79 0.4948061159822288 1102 80-81 0.5189821112280697 1102 82-83 0.5477291850390955 1102 84-85 0.5442461031813295 1102 86-87 0.5143334011310756 1102 88-89 0.5260027568501506 1102 90-91 0.5153710398798701 1102 92-93 0.5085901574823417 1102 94-95 0.4891338818611075 1102 96-97 0.49562288462762183 1102 98-99 0.492380688752764 1102 100 0.5215804323075837 1103 1 1.482123590836757 1103 2 1.1088355263387797 1103 3 1.4247659248358424 1103 4 0.9074679632282923 1103 5 0.5052819878903492 1103 6 1.055295523941247 1103 7 0.771922870066291 1103 8 0.4451364755045262 1103 9 0.469625179591155 1103 10-11 0.3819141288155734 1103 12-13 0.32149950464675925 1103 14-15 0.38245870414295524 1103 16-17 0.41862743007340697 1103 18-19 0.38271179860932847 1103 20-21 0.40375891462772273 1103 22-23 0.4034471748450841 1103 24-25 0.4101463525002842 1103 26-27 0.40700327602921504 1103 28-29 0.413743105417538 1103 30-31 0.3891091310493806 1103 32-33 0.4203722997064574 1103 34-35 0.4377247695180486 1103 36-37 0.408994419098768 1103 38-39 0.4369545008027913 1103 40-41 0.477549660463616 1103 42-43 0.48408590319063904 1103 44-45 0.4484373851968826 1103 46-47 0.47960739839997757 1103 48-49 0.41099015004119366 1103 50-51 0.40088135291077265 1103 52-53 0.41686289013370015 1103 54-55 0.41499912112660553 1103 56-57 0.4167757092002873 1103 58-59 0.41864581569003434 1103 60-61 0.38974356547227984 1103 62-63 0.3355373848495873 1103 64-65 0.31323802372468634 1103 66-67 0.3878473531408133 1103 68-69 0.34752209055815086 1103 70-71 0.3479434313878116 1103 72-73 0.36444936937055417 1103 74-75 0.38709684205105077 1103 76-77 0.2172696421514715 1103 78-79 0.39474140808678726 1103 80-81 0.37312168521900446 1103 82-83 0.3659345246278676 1103 84-85 0.3538834901497623 1103 86-87 0.3518467538699994 1103 88-89 0.369233431858067 1103 90-91 0.34621812639748484 1103 92-93 0.34763946530827994 1103 94-95 0.33867696981960194 1103 96-97 0.370102018497267 1103 98-99 0.37886202053549667 1103 100 0.41850662197205324 1104 1 1.402310629565065 1104 2 1.0331068404848551 1104 3 1.3879373142663987 1104 4 0.8591547057553797 1104 5 0.4604091692889085 1104 6 1.0169926643653113 1104 7 0.7348690540072482 1104 8 0.40382158892490594 1104 9 0.4277784884056075 1104 10-11 0.32539452399736746 1104 12-13 0.29251413266739235 1104 14-15 0.3535327266789281 1104 16-17 0.3736603053233267 1104 18-19 0.33478014704069636 1104 20-21 0.33758850327827616 1104 22-23 0.3598003361760149 1104 24-25 0.33931763702624806 1104 26-27 0.3506839464313103 1104 28-29 0.31546548465384205 1104 30-31 0.3341305130749106 1104 32-33 0.3548162281985512 1104 34-35 0.35082756985374175 1104 36-37 0.33743484254309664 1104 38-39 0.38031980946914246 1104 40-41 0.39479549821938775 1104 42-43 0.3777596930852454 1104 44-45 0.3898497559173961 1104 46-47 0.40571161186232985 1104 48-49 0.36856086808231225 1104 50-51 0.31386180054485635 1104 52-53 0.357862893219135 1104 54-55 0.3660658466221278 1104 56-57 0.41369432174440846 1104 58-59 0.46289580951917486 1104 60-61 0.4457901893954741 1104 62-63 0.37835863975541884 1104 64-65 0.3233273931863394 1104 66-67 0.3248935173350276 1104 68-69 0.25154922382402134 1104 70-71 0.29566424014056025 1104 72-73 0.3065340088689865 1104 74-75 0.28832428756236794 1104 76-77 0.20342362358953636 1104 78-79 0.3478227955426689 1104 80-81 0.3866026820595323 1104 82-83 0.365988596777548 1104 84-85 0.33366175576819757 1104 86-87 0.3620277914381873 1104 88-89 0.34211445153076525 1104 90-91 0.34384981252292945 1104 92-93 0.3544208996626885 1104 94-95 0.3708667916052022 1104 96-97 0.34867375781551146 1104 98-99 0.35672286150012766 1104 100 0.3423301786081936 1105 1 1.367371481492043 1105 2 1.0117249946430675 1105 3 1.3610103365787687 1105 4 0.8417155576823561 1105 5 0.4393374992415815 1105 6 0.9944799868805276 1105 7 0.7139148619856144 1105 8 0.3720061731034079 1105 9 0.336030685836306 1105 10-11 0.2661771473915522 1105 12-13 0.23959197242398744 1105 14-15 0.32653602282497474 1105 16-17 0.30227127557349576 1105 18-19 0.2919065838223034 1105 20-21 0.32390822944460496 1105 22-23 0.31997570804890074 1105 24-25 0.2910130393250938 1105 26-27 0.3122973676618699 1105 28-29 0.3073823203536392 1105 30-31 0.3123734136834244 1105 32-33 0.3693323708625087 1105 34-35 0.3571527895968103 1105 36-37 0.35364807783721375 1105 38-39 0.36656389330957495 1105 40-41 0.36901921356760425 1105 42-43 0.3478196998465606 1105 44-45 0.3263782379998901 1105 46-47 0.355391294080043 1105 48-49 0.3326624569937451 1105 50-51 0.32315481947657076 1105 52-53 0.31454435028472005 1105 54-55 0.3808148324233471 1105 56-57 0.37623404453007225 1105 58-59 0.3178188994448021 1105 60-61 0.2959968323974991 1105 62-63 0.27234782163506566 1105 64-65 0.26577879616132094 1105 66-67 0.27280764850473815 1105 68-69 0.24443039353328544 1105 70-71 0.24537984189850448 1105 72-73 0.24346774788183012 1105 74-75 0.28237770203160295 1105 76-77 0.19464657828865484 1105 78-79 0.28830876579283427 1105 80-81 0.3286171343921893 1105 82-83 0.31415247439755944 1105 84-85 0.3190560255450805 1105 86-87 0.2966039408634771 1105 88-89 0.2908306449046627 1105 90-91 0.2736495082633006 1105 92-93 0.28121941217113644 1105 94-95 0.2753377179067549 1105 96-97 0.27782816958021783 1105 98-99 0.27094868976246644 1105 100 0.2721366356737782 1106 1 1.3631383909053056 1106 2 0.99425076378499 1106 3 1.3345779221357255 1106 4 0.8151273365429645 1106 5 0.4099364587658201 1106 6 0.9548598997644575 1106 7 0.6839400036571277 1106 8 0.32950864557413695 1106 9 0.31219883263518255 1106 10-11 0.2227671180496671 1106 12-13 0.20992591992451537 1106 14-15 0.2685653523833835 1106 16-17 0.265972049437849 1106 18-19 0.22853311220482908 1106 20-21 0.24041413560932057 1106 22-23 0.26768345638298285 1106 24-25 0.24207721169427288 1106 26-27 0.21279955721952604 1106 28-29 0.22311832765177542 1106 30-31 0.24259321963970137 1106 32-33 0.25247821408251525 1106 34-35 0.2791740263224227 1106 36-37 0.28222165863315496 1106 38-39 0.3278970639193801 1106 40-41 0.320457760329937 1106 42-43 0.348434558033226 1106 44-45 0.35598701026796675 1106 46-47 0.36949344555114294 1106 48-49 0.3026048322468995 1106 50-51 0.3296222334374619 1106 52-53 0.31400012265235233 1106 54-55 0.35491189587019534 1106 56-57 0.4156362479548932 1106 58-59 0.40872596292459207 1106 60-61 0.4023595425554163 1106 62-63 0.3994662549233894 1106 64-65 0.33989627647634535 1106 66-67 0.31623393170336556 1106 68-69 0.31278740232333746 1106 70-71 0.3334887939064899 1106 72-73 0.24331201098197397 1106 74-75 0.3279172413641831 1106 76-77 0.22243879754108065 1106 78-79 0.36489668401547704 1106 80-81 0.39898125805459017 1106 82-83 0.4164367263391995 1106 84-85 0.39404321719188573 1106 86-87 0.39096773814354435 1106 88-89 0.379151732863253 1106 90-91 0.3799513122623601 1106 92-93 0.40442829299508887 1106 94-95 0.37844915767090725 1106 96-97 0.360021311003365 1106 98-99 0.35791954549977234 1106 100 0.4027357796003308 1107 1 1.288156531931378 1107 2 0.9144428643372393 1107 3 1.2609946112358763 1107 4 0.7807280083833419 1107 5 0.3857086675406691 1107 6 0.9245384236808789 1107 7 0.6544168710155773 1107 8 0.2906154459625796 1107 9 0.29110050178096003 1107 10-11 0.19823611204713387 1107 12-13 0.11935690825939105 1107 14-15 0.16984964227415844 1107 16-17 0.19042421132703424 1107 18-19 0.15381144275857395 1107 20-21 0.15803775497298744 1107 22-23 0.20412135541430132 1107 24-25 0.18618453559491144 1107 26-27 0.17421109015482728 1107 28-29 0.1784239343180758 1107 30-31 0.18427018268363327 1107 32-33 0.20072054669402917 1107 34-35 0.22551453669442623 1107 36-37 0.2318737335924439 1107 38-39 0.19815164264613827 1107 40-41 0.2071955431515491 1107 42-43 0.24147091880426075 1107 44-45 0.19720872815546642 1107 46-47 0.26034750937969875 1107 48-49 0.2496523535582753 1107 50-51 0.22788893373470387 1107 52-53 0.22070159486956697 1107 54-55 0.23085402998126625 1107 56-57 0.20640948671209713 1107 58-59 0.17889236759805272 1107 60-61 0.14910327936755152 1107 62-63 0.14246197371501523 1107 64-65 0.13823678718581078 1107 66-67 0.1356250119148399 1107 68-69 0.143973944319022 1107 70-71 0.1471837977193431 1107 72-73 0.1421566836899828 1107 74-75 0.12326995625380022 1107 76-77 -0.02699066753220336 1107 78-79 0.10128416597019196 1107 80-81 0.18865587901228764 1107 82-83 0.1993813489537004 1107 84-85 0.19919121189217037 1107 86-87 0.1347790946781373 1107 88-89 0.16220332638242851 1107 90-91 0.16063395958545001 1107 92-93 0.17166268132483609 1107 94-95 0.13057200933106117 1107 96-97 0.16209628402536325 1107 98-99 0.20132545361278886 1107 100 0.19626286014311134 1108 1 1.2966199895359196 1108 2 0.9093045456005981 1108 3 1.2520436055177804 1108 4 0.7473406130941456 1108 5 0.36993956741304856 1108 6 0.8867847066556678 1108 7 0.6347883310484548 1108 8 0.28509825568568914 1108 9 0.2416431166541244 1108 10-11 0.1476737614695125 1108 12-13 0.13637110978913825 1108 14-15 0.15328337389451008 1108 16-17 0.15818357163934849 1108 18-19 0.09986842785058059 1108 20-21 0.1468304669481526 1108 22-23 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-1.2249591557563697 2316 100 -1.130422583238012 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 48.0 7 669.0 8 859.0 9 330.0 10 321.0 11 579.0 12 752.0 13 1197.0 14 2698.0 15 6701.0 16 14689.0 17 23521.0 18 30138.0 19 38347.0 20 53135.0 21 76322.0 22 114952.0 23 191030.0 24 331944.0 25 457743.0 26 468998.0 27 392595.0 28 329585.0 29 327812.0 30 369076.0 31 443321.0 32 543021.0 33 689763.0 34 939034.0 35 1452849.0 36 2623431.0 37 5304534.0 38 6933328.0 39 1996202.0 40 4580.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 16.899598815779115 33.599033178579454 27.967548355187876 21.53381965045355 2 20.999964117991464 34.195293397913225 26.45728652470828 18.34745595938703 3 26.835150632199788 25.426637619744962 26.448796656815926 21.289415091239327 4 19.783778992179528 27.18244378480995 24.644557392608235 28.389219830402286 5 21.392990889125898 33.16090668749317 25.80767777245675 19.63842465092419 6 29.431858327674288 26.471668448535663 25.12189026887625 18.974582954913803 7 20.781614017214388 25.602286013879592 33.045621238732366 20.570478730173654 8 27.95118897499339 26.201242184205135 25.40034991652268 20.44721892427879 9 27.357372737677345 25.204509962380563 26.70590227554061 20.732215024401484 10-11 20.37919545215912 30.006583738624954 29.304328631230437 20.309892177985493 12-13 20.243954431083164 26.34650870342673 29.305366725179084 24.104170140311023 14-15 20.227066975046952 33.66630519385283 25.707466744887377 20.39916108621284 16-17 24.04047939219581 26.131910655220814 25.752451012241384 24.07515894034199 18-19 20.269450090100587 29.861084441616377 25.78549777802645 24.08396769025659 20-21 23.9576682173607 29.96185154464578 25.74655110145378 20.333929136539744 22-23 23.93875194006844 26.331334720736088 29.37872370644447 20.351189632751005 24-25 20.33963056847687 29.845026427960512 25.61153494884185 24.203808054720763 26-27 20.396403436337117 26.1943625467843 33.017583659044405 20.39165035783418 28-29 20.373013917515244 26.31171205611248 32.99716311695708 20.31811090941519 30-31 20.353261873849547 26.24991086498604 32.983495733914424 20.413331527249987 32-33 20.478136527325606 26.36193640817257 32.78594389873797 20.37398316576386 34-35 20.449028250996236 26.49170620235342 32.700938242629526 20.358327304020822 36-37 20.389609989190685 26.439879040474285 32.76210568337483 20.408405286960203 38-39 20.410892148338096 26.43605219735537 32.780788820551834 20.37226683375471 40-41 20.434294657166664 26.48588690218902 32.72832861991746 20.351489820726858 42-43 20.43813160318298 26.501615289893316 32.67831206862654 20.381941038297168 44-45 20.461370088986488 26.56295362433541 32.63589847698757 20.339777809690528 46-47 20.492130095030507 26.682969663125704 32.53049707062906 20.294403171214725 48-49 20.502935634871847 26.70823504172535 32.43881811856315 20.350011204839664 50-51 20.512796522207346 26.780436300583823 32.33878070299765 20.367986474211186 52-53 20.602394721351885 26.937472268939576 32.042757646008496 20.41737536370004 54-55 20.939228128322675 27.204708586585944 31.24862213378212 20.607441151309256 56-57 21.4229652970391 27.806900532798473 29.944141831736566 20.825992338425866 58-59 21.503380914924815 28.436820632041194 29.159615160790064 20.90018329224393 60-61 21.51751043223232 28.775008047877293 28.750775943681518 20.95670557620887 62-63 21.503251081009996 28.99642344248752 28.505338054722607 20.99498742177988 64-65 21.50743975951314 29.087684073976433 28.429663565356616 20.97521260115381 66-67 21.443692158667865 28.929119992908536 28.632274653131333 20.994913195292266 68-69 21.36043159584676 28.783459908973192 28.897138876438923 20.95896961874113 70-71 21.302998077329516 28.68178851088442 29.072101136972716 20.94311227481335 72-73 21.272074451327786 28.618312545095776 29.075180231432444 21.03443277214399 74-75 21.346964760979002 28.720774159086464 28.87518775821607 21.057073321718462 76-77 21.389868717078183 28.87954095966623 28.617098096335315 21.113492226920272 78-79 21.485705824886374 29.128766977859495 28.166490650313435 21.219036546940696 80-81 21.580142429324262 29.399986365664876 27.702681399228922 21.31718980578194 82-83 21.599558497836043 29.629166915773098 27.307432171147628 21.463842415243228 84-85 21.617714279089682 29.72827936625707 27.053749982403392 21.600256372249856 86-87 21.63849710593001 29.82235131499587 26.83843194763751 21.70071963143661 88-89 21.65398400288013 29.920081898522167 26.624133897226294 21.801800201371414 90-91 21.73055284988539 29.963919934909878 26.399729340505374 21.905797874699363 92-93 21.768988239358134 30.042996529497657 26.226694529185796 21.961320701958414 94-95 21.81408280197959 30.13311611212974 26.032771859674735 22.020029226215932 96-97 21.825251502775753 30.17169645990092 25.868219473860037 22.134832563463288 98-99 21.823289627026597 30.22072670284597 25.779289195776467 22.176694474350967 100 21.80685451085378 30.249914267501783 25.660603767031752 22.282627454612683 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 26157.0 1 19883.5 2 11080.5 3 8134.0 4 7647.0 5 7768.0 6 8141.5 7 8193.0 8 8209.0 9 8682.5 10 9556.5 11 17506.0 12 123670.5 13 146370.0 14 69202.0 15 72894.0 16 82906.0 17 91956.0 18 99445.0 19 107536.0 20 117843.5 21 131838.0 22 150838.0 23 173999.5 24 198126.0 25 223259.5 26 251275.0 27 286122.5 28 329137.0 29 371880.0 30 407841.5 31 443346.5 32 478913.0 33 505819.0 34 529227.5 35 557644.5 36 589344.5 37 618949.5 38 652974.0 39 680900.0 40 695720.0 41 721609.0 42 756405.0 43 767921.0 44 766101.5 45 776727.5 46 787118.5 47 789979.0 48 787645.5 49 782782.5 50 777509.5 51 771988.0 52 762010.5 53 733877.0 54 691307.5 55 648326.0 56 603597.0 57 549481.5 58 488616.5 59 420999.0 60 346804.5 61 278892.5 62 219388.0 63 165461.0 64 121231.0 65 87317.0 66 62306.5 67 45535.0 68 33547.0 69 24620.5 70 18400.5 71 14131.0 72 11144.0 73 8838.5 74 7078.5 75 6176.0 76 4852.5 77 2902.5 78 1623.0 79 1006.0 80 655.5 81 435.0 82 273.0 83 180.0 84 129.5 85 94.0 86 67.5 87 43.5 88 26.0 89 22.5 90 17.0 91 14.5 92 15.0 93 6.5 94 3.5 95 2.5 96 0.5 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3286196748697986 2 0.006526209289614049 3 2.4314495584028277 4 8.980262624262832E-4 5 4.966043847518617E-5 6 0.009137520679434255 7 0.00774289003225611 8 2.2761034301126994E-4 9 0.0 10-11 9.104413720450797E-5 12-13 4.138369872932181E-6 14-15 0.0 16-17 2.4830219237593084E-5 18-19 0.0 20-21 3.517614391992354E-4 22-23 3.310695898345745E-5 24-25 4.676357956413364E-4 26-27 0.0033810481861855915 28-29 0.006180655405224213 30-31 0.005466786602143411 32-33 0.014807087405351341 34-35 0.009449967604840634 36-37 0.004740502689443813 38-39 0.004941213628281024 40-41 0.007730474922637314 42-43 0.018018462426746717 44-45 0.03296211603790482 46-47 0.032314461152790935 48-49 0.03874755712026401 50-51 0.03872479608596288 52-53 0.06099543355714741 54-55 0.03161921501413833 56-57 0.02056562908353647 58-59 0.015643038119683643 60-61 0.016580378895902782 62-63 0.02349766413850892 64-65 0.043872928207890516 66-67 0.04602901891168818 68-69 0.04915969572056138 70-71 0.05942699137530612 72-73 0.06701469253732728 74-75 0.05805719094736557 76-77 0.05750264938439265 78-79 0.0515868496510361 80-81 0.04901692195994522 82-83 0.04596901254853066 84-85 0.04851824839025688 86-87 0.05663773008094983 88-89 0.06288046103426802 90-91 0.058777267305255766 92-93 0.06329429802156124 94-95 0.07155241510299741 96-97 0.07016606119556512 98-99 0.045803477753613375 100 0.0071924868391561305 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.4164104E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.60990926842953 #Duplication Level Percentage of deduplicated Percentage of total 1 67.64252494736223 28.145993257472234 2 16.88871562204946 14.05475849387573 3 5.992890060378963 7.480908350041256 4 2.8784072945698975 4.790810654585566 5 1.6266780146037128 3.3842962298304786 6 0.9951799143396204 2.484560756486105 7 0.7044168736148397 2.0517505538784993 8 0.5475439369312665 1.8226602828950957 9 0.38424866960008336 1.43896970507168 >10 2.0590300973483426 15.460831797551627 >50 0.17948161737301369 5.311519059568375 >100 0.09985869097730712 6.411076353371636 >500 3.375361825964531E-4 0.09060808147697114 >1k 5.972648308177208E-4 0.45348370397643833 >5k 4.969990979665074E-5 0.14146420568284634 >10k+ 3.9759927837233466E-5 6.476308514235374 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1478383 6.118095667855096 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 30226 0.1250863677792481 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 29058 0.1202527517676633 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 28165 0.11655718747113487 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 9.683785502661303E-4 0.0 0.0 4.138369872932181E-6 4.138369872932181E-6 2 9.725169201390625E-4 4.138369872932181E-6 0.0 4.138369872932181E-6 1.2415109618796542E-5 3 9.766552900119946E-4 1.2415109618796542E-5 0.0 8.276739745864362E-6 1.2415109618796542E-5 4 9.807936598849269E-4 3.310695898345745E-5 0.0 8.276739745864362E-6 1.2415109618796542E-5 5 9.807936598849269E-4 4.966043847518617E-5 0.0 1.6553479491728724E-5 1.2415109618796542E-5 6 9.807936598849269E-4 4.966043847518617E-5 0.0 2.4830219237593084E-5 1.2415109618796542E-5 7 9.890703996307912E-4 5.379880834811835E-5 0.0 3.724532885638963E-5 2.4830219237593084E-5 8 9.890703996307912E-4 5.793717822105053E-5 0.0 3.724532885638963E-5 2.8968589110525266E-5 9 9.932087695037233E-4 7.035228783984707E-5 0.0 3.724532885638963E-5 2.8968589110525266E-5 10-11 0.0010139006188683844 7.655984264924535E-5 0.0 4.1383698729321805E-5 4.5522068602253986E-5 12-13 0.001032523283296579 8.897495226804189E-5 0.0 4.5522068602253986E-5 5.586799328458444E-5 14-15 0.0010428692079789094 1.1794354137856716E-4 0.0 4.5522068602253986E-5 6.207554809398271E-5 16-17 0.0010635610573435704 1.262202811244315E-4 0.0 4.5522068602253986E-5 6.41447330304488E-5 18-19 0.0010759761669623669 1.5932724010788894E-4 0.0 4.5522068602253986E-5 6.62139179669149E-5 20-21 0.0011049447560728923 2.0071093883721077E-4 0.0 4.5522068602253986E-5 7.449065771277926E-5 22-23 0.0011815045987221377 2.131260484560073E-4 0.0 4.5522068602253986E-5 8.069821252217751E-5 24-25 0.0015063666337473138 2.3174871288420213E-4 0.0 4.5522068602253986E-5 9.932087695037234E-5 26-27 0.002480952738822842 2.4830219237593083E-4 0.0 4.5522068602253986E-5 1.1173598656916888E-4 28-29 0.00541919534860469 2.6485567186765953E-4 0.0 4.5522068602253986E-5 1.2001272631503324E-4 30-31 0.012619958927506687 2.834783362958544E-4 0.0 4.5522068602253986E-5 1.262202811244315E-4 32-33 0.026895265804186243 2.9796263085111704E-4 0.0 4.5522068602253986E-5 1.3656620580676195E-4 34-35 0.05219312083742066 3.186544802157779E-4 0.0 4.966043847518617E-5 1.4070457567969414E-4 36-37 0.09311332214097406 3.248620350251762E-4 0.0 4.966043847518617E-5 1.4070457567969414E-4 38-39 0.15150572104804713 3.3106958983457444E-4 0.0 5.172962341165226E-5 1.4070457567969414E-4 40-41 0.22896772832959167 3.579689940086336E-4 0.0 5.793717822105053E-5 1.4484294555262633E-4 42-43 0.3249779093816183 3.9521432286502325E-4 0.0 6.62139179669149E-5 1.4691213048909242E-4 44-45 0.4466045999470951 4.096986174202859E-4 0.0 6.62139179669149E-5 1.6139642504435506E-4 46-47 0.5980358303374294 4.428055764037433E-4 0.0 6.62139179669149E-5 1.7794990453608378E-4 48-49 0.7811669739544242 4.7177416551426863E-4 0.0 6.62139179669149E-5 1.8415745934548204E-4 50-51 0.993788141285934 4.966043847518617E-4 0.0 7.035228783984707E-5 1.903650141548803E-4 52-53 1.2333169895312484 5.193654190529886E-4 0.0 7.242147277631316E-5 1.9450338402781248E-4 54-55 1.505861752622816 5.586799328458444E-4 0.0 7.449065771277926E-5 2.0278012377367686E-4 56-57 1.8181245205698502 5.835101520834375E-4 0.0 8.69057673315758E-5 2.0484930871014296E-4 58-59 2.1715764838621783 6.021328165116322E-4 0.0 9.518250707744016E-5 2.0898767858307512E-4 60-61 2.562592430491112 6.18686296003361E-4 0.0 9.518250707744016E-5 2.193336032654056E-4 62-63 2.9819024119412827 6.39378145368022E-4 4.138369872932181E-6 1.0139006188683843E-4 2.193336032654056E-4 64-65 3.4346442144099365 6.559316248597506E-4 4.138369872932181E-6 1.075976166962367E-4 2.2554115807480384E-4 66-67 3.9264439517393237 6.683467344785472E-4 4.138369872932181E-6 1.0966680163270278E-4 2.3381789782066822E-4 68-69 4.4568008811748205 6.86969398906742E-4 4.138369872932181E-6 1.1380517150563497E-4 2.4623300743946473E-4 70-71 5.0204158200941364 6.931769537161403E-4 4.138369872932181E-6 1.2415109618796541E-4 2.565789321217952E-4 72-73 5.615921037254267 7.138688030808012E-4 4.138369872932181E-6 1.2415109618796541E-4 2.772707814864561E-4 74-75 6.24285096604451 7.304222825725299E-4 4.138369872932181E-6 1.2415109618796541E-4 2.834783362958544E-4 76-77 6.898377030656713 7.531833168736569E-4 4.138369872932181E-6 1.2415109618796541E-4 2.9589344591465094E-4 78-79 7.5869852240331355 7.738751662383179E-4 4.138369872932181E-6 1.2415109618796541E-4 3.0830855553344745E-4 80-81 8.303512929757296 7.945670156029787E-4 4.138369872932181E-6 1.2415109618796541E-4 3.1037774046991355E-4 82-83 9.046385498092544 8.152588649676396E-4 4.138369872932181E-6 1.282894660608976E-4 3.1244692540637964E-4 84-85 9.815067010140332 8.338815293958343E-4 4.138369872932181E-6 1.282894660608976E-4 3.1658529527931183E-4 86-87 10.60814007421918 8.421582691416987E-4 4.138369872932181E-6 1.3656620580676195E-4 3.20723665152244E-4 88 11.217692987912981 8.442274540781648E-4 4.138369872932181E-6 1.3656620580676195E-4 3.227928500887101E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 191745 0.0 86.570274 8 AGTGGTA 191975 0.0 85.7486 5 TGGTATC 193655 0.0 85.613914 7 AAGCAGT 187795 0.0 85.46544 1 GTATCAA 194060 0.0 85.42853 9 GTGGTAT 193665 0.0 85.01711 6 GCAGTGG 194180 0.0 84.739845 3 AGCAGTG 200165 0.0 81.96713 2 CAGTGGT 201290 0.0 81.604034 4 CAACGCA 188680 0.0 44.938347 12-13 ATCAACG 190360 0.0 44.63184 10-11 ACGCAGA 190760 0.0 44.532104 14-15 AGAGTAC 192575 0.0 43.987183 18-19 TCAACGC 188740 0.0 43.89817 12-13 AACGCAG 189815 0.0 43.72136 14-15 GAGTACT 187845 0.0 43.69548 20-21 CGCAGAG 192330 0.0 43.15705 16-17 AGTACTT 194520 0.0 43.145657 20-21 TATCAAC 193850 0.0 42.77013 10-11 GTACTTT 196240 0.0 41.76721 22-23 >>END_MODULE