FastQCFastQC Report
Thu 26 May 2016
SRR1660710_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660710_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29145155
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT26765169.183399436372872No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1914110.6567506674780079No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT415000.1423907335541705No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT351770.12069587552373628No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3266800.088.977248
AGTGGTA3281550.088.670165
TGGTATC3285800.088.208417
GTGGTAT3305650.088.01756
GTATCAA3304200.087.856579
GCAGTGG3346950.087.42683
AAGCAGT3347050.087.331411
CAGTGGT3382200.085.9069444
AGCAGTG3438100.085.14582
CAACGCA3231200.045.85251612-13
ATCAACG3245300.045.743810-11
ACGCAGA3262950.045.42460314-15
TCAACGC3231500.045.08017312-13
AGAGTAC3240800.045.03518718-19
AACGCAG3254650.044.834814-15
GAGTACT3192000.044.59552820-21
CGCAGAG3270400.044.5174316-17
AGTACTT3278100.044.1763220-21
TATCAAC3289100.044.0757510-11
CAGAGTA3324650.043.26302718-19
Produced by FastQC (version 0.11.5)