##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660710_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 29145155 Sequences flagged as poor quality 0 Sequence length 100 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.901480674918353 34.0 31.0 34.0 30.0 34.0 2 32.02101718107178 34.0 31.0 34.0 30.0 34.0 3 32.088210201661305 34.0 31.0 34.0 30.0 34.0 4 35.49508122362019 37.0 35.0 37.0 33.0 37.0 5 35.46062280334416 37.0 35.0 37.0 33.0 37.0 6 35.55656382681787 37.0 35.0 37.0 33.0 37.0 7 35.46252040862366 37.0 35.0 37.0 33.0 37.0 8 35.505990515404704 37.0 35.0 37.0 33.0 37.0 9 37.19827604279339 39.0 37.0 39.0 34.0 39.0 10-11 37.065859008126736 39.0 37.0 39.0 33.5 39.0 12-13 37.16212547162641 39.0 37.0 39.0 33.5 39.0 14-15 38.565332574144826 40.0 38.0 41.0 34.0 41.0 16-17 38.5265530583042 40.0 38.0 41.0 34.0 41.0 18-19 38.446171173219014 40.0 38.0 41.0 34.0 41.0 20-21 38.28087620395225 40.0 38.0 41.0 33.5 41.0 22-23 38.03165538148622 40.0 37.5 41.0 32.5 41.0 24-25 38.05960050649928 40.0 38.0 41.0 33.0 41.0 26-27 38.114808121624336 40.0 38.0 41.0 33.0 41.0 28-29 38.0642520686543 40.0 38.0 41.0 33.0 41.0 30-31 37.982038798558456 40.0 38.0 41.0 33.0 41.0 32-33 37.86210413017189 40.0 38.0 41.0 32.0 41.0 34-35 37.71382245179345 40.0 38.0 41.0 32.0 41.0 36-37 37.49162097439523 39.5 37.0 41.0 31.5 41.0 38-39 36.8799775125574 39.0 35.0 41.0 31.0 41.0 40-41 36.81026532540314 39.0 35.0 41.0 30.5 41.0 42-43 36.785556758919284 39.0 35.0 41.0 30.5 41.0 44-45 36.770187532027194 39.0 35.0 41.0 30.0 41.0 46-47 36.836803149614404 40.0 35.0 41.0 31.0 41.0 48-49 36.71714775577622 39.5 35.0 41.0 30.0 41.0 50-51 35.32203566939342 38.0 34.0 40.0 27.5 40.5 52-53 35.39937277739645 38.5 34.5 39.5 27.0 40.5 54-55 35.53422338978811 39.0 35.0 41.0 24.5 41.0 56-57 34.963374547158864 39.0 35.0 41.0 20.0 41.0 58-59 34.75108034251319 39.0 34.5 41.0 18.0 41.0 60-61 34.55620874207051 39.0 34.0 41.0 18.0 41.0 62-63 34.34823077798008 38.0 34.0 41.0 18.0 41.0 64-65 34.11680914031852 37.5 34.0 40.5 18.0 41.0 66-67 33.88127530973844 37.0 34.0 40.0 18.0 41.0 68-69 33.648560661283156 36.5 33.0 40.0 18.0 41.0 70-71 33.388912959975684 36.0 33.0 39.0 19.0 41.0 72-73 33.07161233144926 35.5 33.0 39.0 18.0 41.0 74-75 32.699143442537874 35.0 33.0 39.0 17.5 40.5 76-77 32.29634347458437 35.0 32.0 37.5 15.0 40.0 78-79 31.905749137378066 35.0 32.0 37.0 14.0 39.0 80-81 31.56394321800656 35.0 32.0 36.5 11.5 39.0 82-83 31.2311335280255 35.0 31.0 36.0 10.0 38.0 84-85 30.93156624145591 35.0 31.0 36.0 10.0 37.0 86-87 30.70268193804425 35.0 31.0 35.0 10.0 37.0 88-89 30.484963538536682 35.0 31.0 35.0 10.0 36.5 90-91 30.298218554679156 35.0 31.0 35.0 10.0 36.0 92-93 30.128736268515297 35.0 30.0 35.0 10.0 36.0 94-95 29.93705233339812 35.0 30.0 35.0 10.0 36.0 96-97 29.790566013459184 35.0 30.0 35.0 10.0 35.0 98-99 29.678730118264937 34.0 29.0 35.0 10.0 35.0 100 29.593356563037663 34.0 29.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5258605212317278 1101 2 0.5441265414432692 1101 3 0.5397675279899445 1101 4 0.4716151497498231 1101 5 0.4894736726501634 1101 6 0.45510663633015014 1101 7 0.45978997042806213 1101 8 0.48173424512967955 1101 9 0.5584226428754704 1101 10-11 0.5981368191870899 1101 12-13 0.5712267460570999 1101 14-15 0.6733619093532255 1101 16-17 0.6588839634383561 1101 18-19 0.6555136816449121 1101 20-21 0.6887552699010513 1101 22-23 0.777423534975128 1101 24-25 0.7646944355214842 1101 26-27 0.7029690797615231 1101 28-29 0.7152945259492967 1101 30-31 0.6958012316364872 1101 32-33 0.6859935164612594 1101 34-35 0.7228350011433164 1101 36-37 0.6699653333570623 1101 38-39 0.7187038612624335 1101 40-41 0.7102624652059717 1101 42-43 0.689911788343899 1101 44-45 0.7453478782398761 1101 46-47 0.783386422233761 1101 48-49 0.8121391941184797 1101 50-51 0.7657585288096129 1101 52-53 0.7939348261501706 1101 54-55 0.8305550165985096 1101 56-57 0.8082805177292798 1101 58-59 0.70672014552067 1101 60-61 0.6749030612940672 1101 62-63 0.6442043203156587 1101 64-65 0.6352695715228478 1101 66-67 0.6361846318427595 1101 68-69 0.6029796239012555 1101 70-71 0.5098673101358173 1101 72-73 0.5117773997143331 1101 74-75 0.5217568301768409 1101 76-77 0.47640047950775255 1101 78-79 0.4544215432889267 1101 80-81 0.4047082410234175 1101 82-83 0.43366661554405184 1101 84-85 0.40313885164998453 1101 86-87 0.38346677231204396 1101 88-89 0.37966262052809086 1101 90-91 0.3611279908376055 1101 92-93 0.35804416954932705 1101 94-95 0.43585539389967565 1101 96-97 0.441470132930597 1101 98-99 0.38282072905964526 1101 100 0.39033910105359837 1102 1 0.44357617374740954 1102 2 0.4888633137850391 1102 3 0.4928287731568304 1102 4 0.4176795661852921 1102 5 0.4235721704872901 1102 6 0.3886520510234135 1102 7 0.4007591106713093 1102 8 0.420528939305683 1102 9 0.49233674546598394 1102 10-11 0.49586019391702507 1102 12-13 0.4999696749783098 1102 14-15 0.5951888156489744 1102 16-17 0.5768152348569231 1102 18-19 0.5540323879645328 1102 20-21 0.5845362185269494 1102 22-23 0.6268566656228742 1102 24-25 0.5649238529106526 1102 26-27 0.5672864682368086 1102 28-29 0.5842191785514856 1102 30-31 0.5530954502835002 1102 32-33 0.5621917319444876 1102 34-35 0.567629115890881 1102 36-37 0.554861437387153 1102 38-39 0.6102265983021908 1102 40-41 0.5497513326829164 1102 42-43 0.5767975992458005 1102 44-45 0.6125653623611882 1102 46-47 0.6212933757817893 1102 48-49 0.6462580081457077 1102 50-51 0.6173090117038242 1102 52-53 0.6773714283901597 1102 54-55 0.7471888634845882 1102 56-57 0.7253970610316784 1102 58-59 0.5825837080609162 1102 60-61 0.6158410518846935 1102 62-63 0.5929914616112342 1102 64-65 0.5446850647782924 1102 66-67 0.49823559191086275 1102 68-69 0.49183629260735273 1102 70-71 0.49792615501092996 1102 72-73 0.47541069620494625 1102 74-75 0.45802791053458947 1102 76-77 0.48966213821447013 1102 78-79 0.45809938708771725 1102 80-81 0.3956743439211543 1102 82-83 0.43039443203734606 1102 84-85 0.401719718013414 1102 86-87 0.3688895220161861 1102 88-89 0.41624930074657485 1102 90-91 0.3739571847915677 1102 92-93 0.33519859081586034 1102 94-95 0.3980663485270277 1102 96-97 0.35955492661592103 1102 98-99 0.31295790368327303 1102 100 0.37479908169108 1103 1 0.41350933094520315 1103 2 0.4372153905581726 1103 3 0.45952817484511144 1103 4 0.3969623101826443 1103 5 0.41214312839849754 1103 6 0.36743646239492733 1103 7 0.3846774716393355 1103 8 0.38626044032258733 1103 9 0.46688038758560424 1103 10-11 0.4768659404972695 1103 12-13 0.4737727030734078 1103 14-15 0.5540299114063103 1103 16-17 0.5190749439587421 1103 18-19 0.5290270764432776 1103 20-21 0.5593340533692626 1103 22-23 0.604539744151019 1103 24-25 0.6466068765284376 1103 26-27 0.5640857223007103 1103 28-29 0.572324686694067 1103 30-31 0.5420646138931389 1103 32-33 0.4745294348752296 1103 34-35 0.5228793064931878 1103 36-37 0.5045730946451457 1103 38-39 0.5568204876240301 1103 40-41 0.5583092599435844 1103 42-43 0.5853010807737391 1103 44-45 0.5676954048744776 1103 46-47 0.6375470954306195 1103 48-49 0.6661538474188688 1103 50-51 0.5776264569360166 1103 52-53 0.6016379843913384 1103 54-55 0.7264288196407804 1103 56-57 0.7935300243536787 1103 58-59 0.6804481597281864 1103 60-61 0.6730350269511618 1103 62-63 0.6420256517573648 1103 64-65 0.650016058085555 1103 66-67 0.6185092778109365 1103 68-69 0.6045549255928577 1103 70-71 0.5978072952034097 1103 72-73 0.550188129737009 1103 74-75 0.5745628345787779 1103 76-77 0.5731692839529003 1103 78-79 0.5395961378728238 1103 80-81 0.5084192916401555 1103 82-83 0.5155175473976747 1103 84-85 0.5089754249948406 1103 86-87 0.46071336958502584 1103 88-89 0.49281729772227933 1103 90-91 0.5072930313297839 1103 92-93 0.4773197779778222 1103 94-95 0.5165020791199098 1103 96-97 0.5294668588480924 1103 98-99 0.5148894177941763 1103 100 0.4656081858285752 1104 1 0.35600245120514984 1104 2 0.4141846264506981 1104 3 0.3895093504829603 1104 4 0.3367366129079201 1104 5 0.3460186479494993 1104 6 0.28441598132170043 1104 7 0.3067316819009136 1104 8 0.32250186590226093 1104 9 0.38044058862031704 1104 10-11 0.3509250776977453 1104 12-13 0.3528350519803851 1104 14-15 0.4387019202346707 1104 16-17 0.4047829336534079 1104 18-19 0.4211442110716135 1104 20-21 0.3849594304486814 1104 22-23 0.4464506607483685 1104 24-25 0.45039767948293274 1104 26-27 0.3865454435998217 1104 28-29 0.3851461395000868 1104 30-31 0.3896383751567569 1104 32-33 0.3524589919105381 1104 34-35 0.35973488559660893 1104 36-37 0.3878324263593811 1104 38-39 0.3862128550488251 1104 40-41 0.3653028239903051 1104 42-43 0.3798374594457883 1104 44-45 0.40835078807077707 1104 46-47 0.4221292879462979 1104 48-49 0.5144768907511263 1104 50-51 0.43771720680329906 1104 52-53 0.4421432882354921 1104 54-55 0.5408453828500868 1104 56-57 0.5615848031081967 1104 58-59 0.5247418978062015 1104 60-61 0.5354119823648773 1104 62-63 0.48963286771640924 1104 64-65 0.5033057790920736 1104 66-67 0.5267797216116463 1104 68-69 0.5120736907369903 1104 70-71 0.4836529911683698 1104 72-73 0.4998465184217693 1104 74-75 0.4769203943506639 1104 76-77 0.4171463240832054 1104 78-79 0.4536698357383351 1104 80-81 0.4041112019018662 1104 82-83 0.4152562496453136 1104 84-85 0.41930177896251664 1104 86-87 0.3590987381370532 1104 88-89 0.3741885500927431 1104 90-91 0.3483636233845395 1104 92-93 0.4101670777059745 1104 94-95 0.4513558524221999 1104 96-97 0.4118679752121892 1104 98-99 0.37706915631529014 1104 100 0.3161889264578477 1105 1 0.3019414030342418 1105 2 0.3286530619205692 1105 3 0.3249020897200552 1105 4 0.3027806873967478 1105 5 0.31072248471816977 1105 6 0.29508075902327136 1105 7 0.29896221774338017 1105 8 0.3029434359249521 1105 9 0.3117547683979751 1105 10-11 0.34663205290326715 1105 12-13 0.31142412421629473 1105 14-15 0.3625612211019913 1105 16-17 0.34502335829800757 1105 18-19 0.3516959445870711 1105 20-21 0.36885827171244756 1105 22-23 0.4369372956130846 1105 24-25 0.4426925398507109 1105 26-27 0.4199370343283775 1105 28-29 0.3978457380971534 1105 30-31 0.3855135670269263 1105 32-33 0.36569666085853925 1105 34-35 0.3720607705699166 1105 36-37 0.3905027685216069 1105 38-39 0.41438117399463437 1105 40-41 0.43891223190219364 1105 42-43 0.4653405010045688 1105 44-45 0.47420435227201807 1105 46-47 0.504338631765819 1105 48-49 0.5285483603283794 1105 50-51 0.4213800265606551 1105 52-53 0.4653250140467975 1105 54-55 0.5265393067799025 1105 56-57 0.5203809846648042 1105 58-59 0.47851925475676893 1105 60-61 0.45130879890265163 1105 62-63 0.4660259505708524 1105 64-65 0.46783555755450834 1105 66-67 0.44463122268623323 1105 68-69 0.46681402088815815 1105 70-71 0.47325022051360577 1105 72-73 0.43831255317361695 1105 74-75 0.4254239940305027 1105 76-77 0.41561654582223184 1105 78-79 0.43556620269788837 1105 80-81 0.3913330512130315 1105 82-83 0.3991371664914318 1105 84-85 0.3764035767901035 1105 86-87 0.34278145716379527 1105 88-89 0.3947513559481628 1105 90-91 0.360343657137399 1105 92-93 0.32273060285146116 1105 94-95 0.3544733485723448 1105 96-97 0.3613375960248 1105 98-99 0.3714451897196689 1105 100 0.36615259446226744 1106 1 0.24426893585092557 1106 2 0.2816629966176265 1106 3 0.26566667386011034 1106 4 0.250115472842559 1106 5 0.28027301035728414 1106 6 0.19999938380996696 1106 7 0.2547223239486698 1106 8 0.2398896039864411 1106 9 0.2521755092426048 1106 10-11 0.26437944822843207 1106 12-13 0.25284833209099133 1106 14-15 0.31872524489978815 1106 16-17 0.30561713858718065 1106 18-19 0.29289100243666155 1106 20-21 0.33410072390518053 1106 22-23 0.3231955446282839 1106 24-25 0.3184951924780606 1106 26-27 0.32702084525711683 1106 28-29 0.33778712772156183 1106 30-31 0.3238558488120944 1106 32-33 0.3324321730034967 1106 34-35 0.33503328086879236 1106 36-37 0.30224420929858553 1106 38-39 0.3522773378898947 1106 40-41 0.38065482446463506 1106 42-43 0.38179921349660617 1106 44-45 0.38426426631856714 1106 46-47 0.4146533941829631 1106 48-49 0.4144332563872126 1106 50-51 0.31951629368867174 1106 52-53 0.39531170654951353 1106 54-55 0.5330666220866078 1106 56-57 0.5696483618655961 1106 58-59 0.48776097939425966 1106 60-61 0.47212953254844336 1106 62-63 0.4500794563203101 1106 64-65 0.45203828332869733 1106 66-67 0.46600486903397353 1106 68-69 0.4249190534288658 1106 70-71 0.44742558605668137 1106 72-73 0.4163948972898197 1106 74-75 0.39605447336922595 1106 76-77 0.42055166121840415 1106 78-79 0.4273258003393927 1106 80-81 0.35682932000157663 1106 82-83 0.35898818751919137 1106 84-85 0.39712352969147346 1106 86-87 0.32944313488684074 1106 88-89 0.3487503869408606 1106 90-91 0.3482599620368241 1106 92-93 0.30632379269091814 1106 94-95 0.4059310289609286 1106 96-97 0.4024158676673011 1106 98-99 0.4023905501199714 1106 100 0.3906467614630742 1107 1 0.19540421881888648 1107 2 0.22711598625541995 1107 3 0.21312922759900488 1107 4 0.2177828769605341 1107 5 0.23943940794305263 1107 6 0.18283395064410968 1107 7 0.17715386032547542 1107 8 0.21139212097695292 1107 9 0.2386914396401778 1107 10-11 0.20535593186347256 1107 12-13 0.21451953426876713 1107 14-15 0.26174755840882113 1107 16-17 0.23074824617637546 1107 18-19 0.2396760468892225 1107 20-21 0.24416027931090412 1107 22-23 0.27897855548998507 1107 24-25 0.3068387445630023 1107 26-27 0.2713076839402859 1107 28-29 0.2536464443080604 1107 30-31 0.23903494310208373 1107 32-33 0.23282283524743974 1107 34-35 0.26273905984904644 1107 36-37 0.26904857634412593 1107 38-39 0.27888065137097584 1107 40-41 0.31055037307820044 1107 42-43 0.33620611887332075 1107 44-45 0.33826670573762385 1107 46-47 0.3058877689516777 1107 48-49 0.3399966025432377 1107 50-51 0.2670764693402816 1107 52-53 0.28985483462252404 1107 54-55 0.3443108371248158 1107 56-57 0.334424693657013 1107 58-59 0.26733509850163983 1107 60-61 0.2788689392831927 1107 62-63 0.25927266522762693 1107 64-65 0.2530223801396545 1107 66-67 0.27073352619618873 1107 68-69 0.19859311504335864 1107 70-71 0.24332837407938257 1107 72-73 0.2649715802394681 1107 74-75 0.29774629034607614 1107 76-77 0.23707996340088044 1107 78-79 0.23726626045037236 1107 80-81 0.1933343842413855 1107 82-83 0.24321586209270052 1107 84-85 0.2484656110003307 1107 86-87 0.2175990666023928 1107 88-89 0.22436019826772124 1107 90-91 0.24159882187504778 1107 92-93 0.20296449933121252 1107 94-95 0.20049622212607332 1107 96-97 0.28669400953904756 1107 98-99 0.2610859375267971 1107 100 0.2523455687757803 1108 1 0.10954855372139605 1108 2 0.13620012283409721 1108 3 0.16302697998983007 1108 4 0.14436909176865953 1108 5 0.11979850014799354 1108 6 0.09774689298322414 1108 7 0.11526224378240357 1108 8 0.15815827845735697 1108 9 0.17612671320279105 1108 10-11 0.11528614711104268 1108 12-13 0.13179713560406725 1108 14-15 0.18271652091462443 1108 16-17 0.18338606526842227 1108 18-19 0.16101183421563547 1108 20-21 0.16522917497563583 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0.09681793275010975 1108 88-89 0.09856566421255764 1108 90-91 0.007293599204750478 1108 92-93 0.06327152483074983 1108 94-95 0.12389673831380676 1108 96-97 0.09456686278844728 1108 98-99 0.0664222398082579 1108 100 0.0923559311331772 1109 1 0.04367977071887452 1109 2 0.09494259988722575 1109 3 0.09665235653893234 1109 4 0.08900235432403036 1109 5 0.12698757909146963 1109 6 0.04439684337663863 1109 7 0.057786215004455244 1109 8 0.07662621356125499 1109 9 0.07119158215920152 1109 10-11 0.07206828681973576 1109 12-13 0.1015139492754713 1109 14-15 0.07421271433999266 1109 16-17 0.05834034016565681 1109 18-19 0.09442613983348735 1109 20-21 0.054518557911166 1109 22-23 0.082215548174851 1109 24-25 0.10956747397703026 1109 26-27 0.10511032476597393 1109 28-29 0.0656213768708156 1109 30-31 0.05306259316364503 1109 32-33 0.02602332414907238 1109 34-35 0.037575187262334 1109 36-37 0.044094167249689065 1109 38-39 0.09022959552384435 1109 40-41 0.09774988274335072 1109 42-43 0.11065452990570179 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-1.1210551229746422 2316 80-81 -1.1952781539856439 2316 82-83 -1.1153444742392615 2316 84-85 -1.2084107082156201 2316 86-87 -1.3736264848942348 2316 88-89 -1.347222301368582 2316 90-91 -1.2610562535663519 2316 92-93 -1.1213935824466184 2316 94-95 -1.3219744792313541 2316 96-97 -1.48503704142135 2316 98-99 -1.3694507961569222 2316 100 -1.1617517794660515 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15068.0 3 3720.0 4 2663.0 5 2197.0 6 2931.0 7 9943.0 8 19936.0 9 24820.0 10 27564.0 11 30328.0 12 36147.0 13 45150.0 14 57140.0 15 72603.0 16 87965.0 17 105414.0 18 117402.0 19 132740.0 20 152631.0 21 187419.0 22 252351.0 23 380383.0 24 590405.0 25 749800.0 26 715762.0 27 579122.0 28 470701.0 29 427897.0 30 451104.0 31 531409.0 32 648681.0 33 827154.0 34 1130153.0 35 1774084.0 36 2956376.0 37 5422826.0 38 7531965.0 39 2564964.0 40 6237.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 15.871066103239142 38.72475912288256 26.92814865909665 18.47602611478165 2 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24.10733365790788 20-21 24.099911329691288 31.723558222755997 25.477998931970724 18.698531515581983 22-23 24.083658766514596 26.382994281095133 30.82027330342453 18.71307364896574 24-25 18.687861016283293 31.675463798187213 25.414885230433526 24.221789955095968 26-27 18.684422522583226 26.342429536653878 36.20939283253218 18.76375510823072 28-29 18.738740845851748 26.382797228274956 36.17246389892564 18.705998026947647 30-31 18.737768330794207 26.389483275216662 36.13422272151437 18.73852567247476 32-33 18.76689417491751 26.42338516683757 36.06939382329095 18.74032683495396 34-35 18.79303070032845 26.501167692940765 35.9912937421595 18.714507864571285 36-37 18.768203662998932 26.48764553399461 35.97371810252515 18.770432700481315 38-39 18.80731083097181 26.465530415518106 35.93209722168963 18.795061531820455 40-41 18.818263584755073 26.510825172255238 35.89133140452687 18.77957983846282 42-43 18.857772672074578 26.54688844889202 35.84807565077402 18.74726322825938 44-45 18.89328147651372 26.614504273751656 35.74434274804681 18.74787150168781 46-47 18.82351410003188 26.68762432153659 35.730147620527276 18.758713957904256 48-49 18.904859764126208 26.717057395456656 35.576624468513685 18.801458371903447 50-51 18.921185643495242 26.79411403376122 35.465914341403256 18.818785981340287 52-53 19.047893658262986 27.014710551999045 35.07653406006529 18.860861729672678 54-55 19.548522285957603 27.454615152253265 33.88357531080495 19.113287250984186 56-57 20.157049724449745 28.27639359725057 32.158987359841774 19.40756931845791 58-59 20.313087211548204 28.99696275285298 31.205983740793545 19.48396629480527 60-61 20.413177080089156 29.412298295983852 30.66619240386445 19.508332220062545 62-63 20.46233545743238 29.622764639042217 30.4019102842911 19.512989619234304 64-65 20.522241465975284 29.66501213290787 30.384589528960948 19.428156872155895 66-67 20.548758403922662 29.41145723875106 30.651789952540987 19.38799440478529 68-69 20.579428533382174 29.10177533710854 31.015120699719596 19.3036754297897 70-71 20.595453395184368 28.886958286693464 31.319218163827365 19.1983701542948 72-73 20.68856750466248 28.77548022799539 31.382361784414698 19.15359048292743 74-75 20.83621791939411 28.875831277318838 31.18292247879736 19.10502832448969 76-77 21.101291519481 29.06818812303868 30.736180943533604 19.094339413946724 78-79 21.376470746244774 29.326199136533145 30.200254347175754 19.097075770046327 80-81 21.645025592801172 29.576719342281443 29.69302652541569 19.0852285395017 82-83 21.841825142518736 29.825526128402103 29.232507765923156 19.100140963156008 84-85 21.9503020881686 29.960147988387835 28.954753261141423 19.13479666230214 86-87 22.111752903336054 29.9886544229439 28.764365172294116 19.135227501425927 88-89 22.364713181067742 30.040164688295345 28.55169757777548 19.04342455286143 90-91 22.602930050715536 30.01277457800564 28.34558872291123 19.038706648367594 92-93 22.80401598191626 29.99109361204567 28.215719138212947 18.989171267825125 94-95 23.028567937897336 29.98700857681988 28.061367562278818 18.923055923003975 96-97 23.201769756477088 29.964559623696736 27.909295050473244 18.924375569352936 98-99 23.382489676120212 29.925410065140802 27.769741365790768 18.922358892948225 100 23.4844137466592 29.912490036558015 27.667917168882312 18.935179047900476 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 147771.0 1 89742.0 2 25996.5 3 19063.5 4 17535.0 5 17200.0 6 17177.5 7 16938.5 8 16798.0 9 16961.0 10 17538.0 11 22958.0 12 227439.0 13 278356.5 14 123722.0 15 124595.0 16 138875.5 17 152124.5 18 162983.5 19 174410.0 20 188796.0 21 208956.5 22 236790.5 23 269624.5 24 303365.0 25 339078.5 26 378484.5 27 426266.0 28 486512.0 29 547506.5 30 597769.0 31 645100.0 32 690420.0 33 723246.5 34 752183.5 35 781673.5 36 814809.5 37 849812.0 38 882495.0 39 899174.5 40 898692.0 41 911352.0 42 934074.5 43 925578.0 44 902935.0 45 899812.5 46 895169.0 47 881587.5 48 856550.5 49 825650.5 50 800033.0 51 775584.5 52 748574.0 53 705800.5 54 650831.5 55 598444.0 56 546010.0 57 490999.0 58 432560.0 59 369426.0 60 304854.5 61 249170.0 62 198310.0 63 148383.0 64 107596.5 65 77495.0 66 56472.5 67 42651.0 68 32404.5 69 24709.0 70 19076.0 71 15009.5 72 12540.5 73 10405.0 74 8640.5 75 7733.5 76 6174.0 77 4055.0 78 2773.0 79 2087.0 80 1553.5 81 1287.0 82 1073.0 83 821.0 84 643.5 85 544.5 86 446.0 87 362.0 88 331.5 89 282.5 90 230.0 91 184.0 92 146.5 93 141.5 94 130.0 95 108.0 96 91.0 97 69.5 98 53.0 99 44.0 100 32.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40668509054077767 2 0.3871140846566093 3 0.16959594141805046 4 0.17575820063403333 5 0.2771026608024558 6 0.10280267852409775 7 0.13719261400393995 8 0.14258630636893166 9 0.13592310625899914 10-11 0.1324199511033652 12-13 0.13413721766104864 14-15 0.1402788902649514 16-17 0.1445729144346633 18-19 0.156192341402885 20-21 0.13775016808110987 22-23 0.09981418867046683 24-25 0.09474816654775038 26-27 0.08911601259283061 28-29 0.09897699977920858 30-31 0.10347860562072839 32-33 0.10451136732674779 34-35 0.10531596074887918 36-37 0.10104252319124739 38-39 0.10020018764696911 40-41 0.1014491087798298 42-43 0.06411014111951026 44-45 0.06716039080938152 46-47 0.08525773837881459 48-49 0.08957921136463333 50-51 0.09898043088122195 52-53 0.10409963508514537 54-55 0.10676045469650101 56-57 0.10745696840521177 58-59 0.10679819681864791 60-61 0.10463317144822183 62-63 0.10908159520853465 64-65 0.11608104331577582 66-67 0.12472913594043333 68-69 0.12982603798126996 70-71 0.12821685113700718 72-73 0.11498995287552939 74-75 0.11255215489504172 76-77 0.11459537614399375 78-79 0.11315088219637191 80-81 0.10740721742601815 82-83 0.11057927123736347 84-85 0.11649277555737825 86-87 0.1296167407584554 88-89 0.13762664840862915 90-91 0.14424695974339474 92-93 0.14371685448233162 94-95 0.13391248047917398 96-97 0.13072327115776192 98-99 0.12581851082967307 100 0.12003367283515906 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.9145155E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.44915002211714 #Duplication Level Percentage of deduplicated Percentage of total 1 68.33797505149238 26.275370549625283 2 16.456301434626724 12.654616053382886 3 6.000619021372184 6.921561029349264 4 2.770567579717599 4.261038740759037 5 1.5068856509129676 2.896923622906416 6 0.9551630829812191 2.2035125203879686 7 0.6873391113467815 1.8499323225768753 8 0.5210763805416639 1.602795514274259 9 0.38998282858263716 1.3495057454001065 >10 2.053654236167489 14.556643550384862 >50 0.18576509253403756 4.961128013022617 >100 0.13012712136149412 8.504178501857758 >500 0.003600620603960824 0.8904777032618237 >1k 7.56569303226435E-4 0.6205679027666223 >5k 1.2414563707123034E-4 0.3003356725843608 >10k+ 6.207281852098357E-5 10.151412557459736 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2676516 9.183399436372872 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 191411 0.6567506674780079 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 41500 0.1423907335541705 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35177 0.12069587552373628 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.04484107221251697 3.431102013353506E-6 0.0 0.0 6.862204026707012E-6 2 0.04762369594534666 6.862204026707012E-6 0.0 0.0 1.0293306040060518E-5 3 0.05117488652916754 6.862204026707012E-6 0.0 0.0 1.0293306040060518E-5 4 0.051662103015063736 6.862204026707012E-6 0.0 0.0 1.0293306040060518E-5 5 0.0517170006472774 6.862204026707012E-6 0.0 0.0 1.0293306040060518E-5 6 0.0517684671774777 1.0293306040060518E-5 0.0 6.862204026707012E-6 1.715551006676753E-5 7 0.0518645380338516 1.3724408053414023E-5 0.0 6.862204026707012E-6 1.715551006676753E-5 8 0.05244782537612169 1.3724408053414023E-5 0.0 1.3724408053414023E-5 2.0586612080121036E-5 9 0.05390604373179693 2.0586612080121036E-5 0.0 1.715551006676753E-5 2.0586612080121036E-5 10-11 0.05425430058615231 3.259546912685831E-5 0.0 1.715551006676753E-5 2.0586612080121036E-5 12-13 0.05435380254453956 4.2888775166918827E-5 0.0 1.8871061073444285E-5 2.744881610682805E-5 14-15 0.054470460012993584 4.975097919362584E-5 0.0 2.0586612080121036E-5 3.945767315356532E-5 16-17 0.05453908205326065 5.661318322033285E-5 0.0 2.401771409347454E-5 4.975097919362584E-5 18-19 0.05459226413446763 6.519093825371661E-5 0.0 2.401771409347454E-5 5.661318322033285E-5 20-21 0.0546317218076212 6.862204026707012E-5 0.0 2.5733265100151296E-5 6.519093825371661E-5 22-23 0.0546831883378215 7.205314228042362E-5 0.0 3.087991812018155E-5 7.205314228042362E-5 24-25 0.054813570214328936 7.205314228042362E-5 0.0 3.087991812018155E-5 7.719979530045387E-5 26-27 0.05513780935459084 7.719979530045387E-5 0.0 3.087991812018155E-5 8.234644832048414E-5 28-29 0.05613969114249007 8.06308973138074E-5 0.0 3.259546912685831E-5 8.577755033383765E-5 30-31 0.05880051075384571 8.406199932716089E-5 0.0 3.431102013353506E-5 8.577755033383765E-5 32-33 0.06407068344635669 9.092420335386791E-5 0.0 3.431102013353506E-5 8.577755033383765E-5 34-35 0.07424390091594985 1.0464861140728193E-4 0.0 3.431102013353506E-5 8.74931013405144E-5 36-37 0.0916550280827122 1.0979526442731219E-4 0.0 3.431102013353506E-5 8.920865234719115E-5 38-39 0.1183850283177427 1.0979526442731219E-4 0.0 3.602657114021181E-5 9.607085637389817E-5 40-41 0.15636218095254598 1.2008857046737271E-4 0.0 4.117322416024207E-5 9.950195838725168E-5 42-43 0.20856125143269955 1.3038187650743323E-4 0.0 4.117322416024207E-5 1.0293306040060518E-4 44-45 0.27674754174407373 1.3381297852078672E-4 0.0 4.117322416024207E-5 1.0636416241395868E-4 46-47 0.3623964257524106 1.38959631540817E-4 0.0 4.4604326173595574E-5 1.0636416241395868E-4 48-49 0.4688240635536164 1.4410628456084724E-4 0.0 4.803542818694908E-5 1.0979526442731219E-4 50-51 0.5984665375771718 1.4925293758087752E-4 0.0 5.146653020030259E-5 1.1494191744734246E-4 52-53 0.7502859394640379 1.561151416075845E-4 0.0 5.8328734227009596E-5 1.1837301946069595E-4 54-55 0.9265759609101409 1.646928966409683E-4 0.0 6.17598362403631E-5 1.200885704673727E-4 56-57 1.128983530881891 1.646928966409683E-4 0.0 6.17598362403631E-5 1.235196724807262E-4 58-59 1.3599138518906488 1.646928966409683E-4 0.0 6.17598362403631E-5 1.3038187650743323E-4 60-61 1.616682086610965 1.6983954966099855E-4 0.0 6.17598362403631E-5 1.3038187650743323E-4 62-63 1.8986998010475498 1.715551006676753E-4 0.0 6.519093825371661E-5 1.38959631540817E-4 64-65 2.210695053774804 1.749862026810288E-4 0.0 6.862204026707012E-5 1.4067518254749374E-4 66-67 2.54962102620487 1.8527950872108931E-4 0.0 6.862204026707012E-5 1.4239073355417047E-4 68-69 2.915438260664594 1.9557281476114985E-4 0.0 6.862204026707012E-5 1.4410628456084724E-4 70-71 3.3109053631727123 2.0243501878785685E-4 0.0 7.033759127374687E-5 1.5096848858755425E-4 72-73 3.732755924612513 2.0586612080121037E-4 0.0 7.205314228042362E-5 1.561151416075845E-4 74-75 4.179473397894093 2.1272832482791736E-4 3.431102013353506E-6 7.205314228042362E-5 1.5954624362093803E-4 76-77 4.650503660042295 2.1615942684127088E-4 3.431102013353506E-6 7.205314228042362E-5 1.646928966409683E-4 78-79 5.152081023415384 2.3159938590136165E-4 6.862204026707012E-6 7.376869328710038E-5 1.646928966409683E-4 80-81 5.679887446129554 2.436082429480989E-4 8.577755033383765E-6 7.719979530045387E-5 1.6812399865432178E-4 82-83 6.235213022541826 2.487548959681292E-4 1.0293306040060518E-5 7.891534630713064E-5 1.6812399865432178E-4 84-85 6.816534343358271 2.590482020081897E-4 1.3724408053414023E-5 7.891534630713064E-5 1.6812399865432178E-4 86-87 7.421664081045375 2.693415080482502E-4 1.3724408053414023E-5 7.891534630713064E-5 1.784173046943823E-4 88 7.892255162135868 2.813503650949875E-4 1.3724408053414023E-5 8.234644832048414E-5 1.8184840670773582E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 326680 0.0 88.97724 8 AGTGGTA 328155 0.0 88.67016 5 TGGTATC 328580 0.0 88.20841 7 GTGGTAT 330565 0.0 88.0175 6 GTATCAA 330420 0.0 87.85657 9 GCAGTGG 334695 0.0 87.4268 3 AAGCAGT 334705 0.0 87.33141 1 CAGTGGT 338220 0.0 85.906944 4 AGCAGTG 343810 0.0 85.1458 2 CAACGCA 323120 0.0 45.852516 12-13 ATCAACG 324530 0.0 45.7438 10-11 ACGCAGA 326295 0.0 45.424603 14-15 TCAACGC 323150 0.0 45.080173 12-13 AGAGTAC 324080 0.0 45.035187 18-19 AACGCAG 325465 0.0 44.8348 14-15 GAGTACT 319200 0.0 44.595528 20-21 CGCAGAG 327040 0.0 44.51743 16-17 AGTACTT 327810 0.0 44.17632 20-21 TATCAAC 328910 0.0 44.07575 10-11 CAGAGTA 332465 0.0 43.263027 18-19 >>END_MODULE