FastQCFastQC Report
Thu 26 May 2016
SRR1660710_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660710_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29145155
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21789607.476234043016755No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1388460.4763947901460809No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT466150.15994082035247367No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT403180.13833517097438663No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2805500.087.5391548
GCAGTGG2783600.087.462083
AAGCAGT2721850.087.1404651
AGTGGTA2797950.087.043145
TGGTATC2830450.086.707877
GTGGTAT2820650.086.307396
GTATCAA2847450.086.130659
AGCAGTG2869650.084.587762
CAGTGGT2902350.083.736824
CAACGCA2762700.045.41856412-13
ATCAACG2775700.045.29969810-11
ACGCAGA2797100.044.9271914-15
TCAACGC2761600.044.383712-13
AGAGTAC2825900.044.3747918-19
AACGCAG2781900.044.12486614-15
GAGTACT2767450.043.9370220-21
CGCAGAG2804700.043.75703416-17
AGTACTT2866150.043.48769820-21
TATCAAC2830250.043.31128710-11
CAGAGTA2910850.042.16464218-19
Produced by FastQC (version 0.11.5)