##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660709_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 23896686 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01483285171844 33.0 31.0 34.0 30.0 34.0 2 32.166616241264585 34.0 31.0 34.0 30.0 34.0 3 32.18287866359377 34.0 31.0 34.0 30.0 34.0 4 35.56053939864297 37.0 35.0 37.0 35.0 37.0 5 35.56141638217115 37.0 35.0 37.0 35.0 37.0 6 35.60623238720214 37.0 35.0 37.0 35.0 37.0 7 35.56668414189315 37.0 35.0 37.0 35.0 37.0 8 35.606431996470135 37.0 35.0 37.0 35.0 37.0 9 37.34933655654177 39.0 38.0 39.0 35.0 39.0 10-11 37.28668224121119 39.0 37.0 39.0 34.5 39.0 12-13 37.27919436192951 39.0 37.0 39.0 34.5 39.0 14-15 38.67030229631004 40.0 38.0 41.0 35.0 41.0 16-17 38.6028315599912 40.0 38.0 41.0 34.5 41.0 18-19 38.53331173201171 40.0 38.0 41.0 34.0 41.0 20-21 38.3120642962794 40.0 38.0 41.0 34.0 41.0 22-23 38.072925844194465 40.0 37.5 41.0 33.0 41.0 24-25 38.108545846064175 40.0 38.0 41.0 34.0 41.0 26-27 37.99207348249041 40.0 38.0 41.0 33.0 41.0 28-29 37.841549932907014 40.0 37.5 41.0 33.0 41.0 30-31 37.62201342479037 40.0 37.0 41.0 32.0 41.0 32-33 37.41755132908388 40.0 37.0 41.0 31.5 41.0 34-35 37.20158586006444 40.0 37.0 41.0 30.5 41.0 36-37 36.86095038450102 39.5 36.0 40.5 30.0 41.0 38-39 36.189936734323744 38.5 35.0 40.0 30.0 41.0 40-41 36.03491258160232 38.0 35.0 40.0 30.0 41.0 42-43 35.943933418215394 38.5 35.0 40.0 29.5 41.0 44-45 35.86204243969227 38.0 35.0 40.0 28.5 41.0 46-47 35.79048799904723 38.0 35.0 40.0 28.0 41.0 48-49 35.65559764228395 39.0 35.0 40.0 27.0 41.0 50-51 34.12524424934905 36.5 33.0 39.0 25.0 40.0 52-53 34.11528142437826 37.5 33.0 39.0 23.0 39.5 54-55 34.52220079805208 38.0 34.0 40.0 21.5 41.0 56-57 33.88963467570357 38.0 33.0 40.0 15.0 41.0 58-59 33.312030567753204 38.0 32.0 40.0 10.0 41.0 60-61 32.797360813963905 38.0 31.0 40.0 9.0 41.0 62-63 32.175877650984745 37.0 30.5 39.5 8.0 41.0 64-65 31.58768060977158 36.0 30.0 39.0 8.0 40.5 66-67 30.937393662033305 35.5 29.0 38.5 8.0 39.0 68-69 30.380796023348175 35.0 28.5 37.0 7.5 39.0 70-71 29.828410830690082 34.0 28.0 36.5 7.0 38.0 72-73 29.294113522686786 34.0 26.5 36.0 7.0 37.0 74-75 28.83341346996818 34.0 26.0 35.0 7.0 37.0 76-77 28.34640663144672 34.0 26.0 35.0 7.0 36.0 78-79 28.08606040603287 33.5 25.5 35.0 7.0 36.0 80-81 27.888647593226942 33.0 25.0 35.0 7.0 35.5 82-83 27.583786199475526 33.0 25.0 35.0 7.0 35.0 84-85 27.311761199858424 33.0 24.0 35.0 7.0 35.0 86-87 27.15831647116257 32.5 23.5 35.0 7.0 35.0 88-89 26.924177101377154 32.0 21.5 35.0 7.0 35.0 90-91 26.75482278170287 32.0 20.0 35.0 7.0 35.0 92-93 26.539658427951053 32.0 20.0 34.5 7.0 35.0 94-95 26.427017013990977 32.0 19.5 34.0 7.0 35.0 96-97 26.184612481412692 31.0 19.0 34.0 7.0 35.0 98-99 25.886172835848452 31.0 19.0 34.0 7.0 35.0 100 25.810489036011102 31.0 19.0 34.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3799043492887435 1101 2 0.4301679445908846 1101 3 0.42172154155697683 1101 4 0.3520422745397269 1101 5 0.34921321923865634 1101 6 0.31878516383515176 1101 7 0.33850067500596026 1101 8 0.3552288311179552 1101 9 0.4150438920340562 1101 10-11 0.4283489433852097 1101 12-13 0.4124272698099887 1101 14-15 0.5081948994460745 1101 16-17 0.4648989052616628 1101 18-19 0.4614717628737779 1101 20-21 0.4913219336102088 1101 22-23 0.5213977451207228 1101 24-25 0.5571533156555972 1101 26-27 0.5805772220615779 1101 28-29 0.6097649265828622 1101 30-31 0.5792017679461381 1101 32-33 0.5551721526472875 1101 34-35 0.5396447379415861 1101 36-37 0.5112841344427679 1101 38-39 0.4877369542391534 1101 40-41 0.48268712809193204 1101 42-43 0.5185690482421919 1101 44-45 0.5623917113555308 1101 46-47 0.5913625245533538 1101 48-49 0.6832665510138227 1101 50-51 0.6265849211876642 1101 52-53 0.6697001922052834 1101 54-55 0.6860769685625812 1101 56-57 0.6548852776376819 1101 58-59 0.5590533813936815 1101 60-61 0.5294829668636254 1101 62-63 0.5198188251772038 1101 64-65 0.47036778523285605 1101 66-67 0.45867233238987737 1101 68-69 0.4766105628588875 1101 70-71 0.48855543753183994 1101 72-73 0.4592381072118066 1101 74-75 0.428195447069843 1101 76-77 0.46353282164472276 1101 78-79 0.457191454734204 1101 80-81 0.4261619258680476 1101 82-83 0.4385404172376326 1101 84-85 0.43534327427554587 1101 86-87 0.42124983997162246 1101 88-89 0.42832071313895526 1101 90-91 0.4297951734755614 1101 92-93 0.41400225938921054 1101 94-95 0.5304676765225587 1101 96-97 0.47144644605075214 1101 98-99 0.4276834174876889 1101 100 0.4640916926671146 1102 1 0.35506525199664907 1102 2 0.3839932777225741 1102 3 0.36418210423630626 1102 4 0.3335971264025446 1102 5 0.31060382978821366 1102 6 0.2682405397795762 1102 7 0.28902965801262326 1102 8 0.30354400186648434 1102 9 0.37164139549338415 1102 10-11 0.3692107372700164 1102 12-13 0.3742240972220898 1102 14-15 0.4400051293623477 1102 16-17 0.3984093181022601 1102 18-19 0.3450918471695843 1102 20-21 0.3599925846983041 1102 22-23 0.4203403298448052 1102 24-25 0.415221794151897 1102 26-27 0.4171410367219863 1102 28-29 0.43434508764163837 1102 30-31 0.42003606818739314 1102 32-33 0.43581260751881246 1102 34-35 0.4801945767118738 1102 36-37 0.4734072737648489 1102 38-39 0.4656754079043992 1102 40-41 0.4557716934302576 1102 42-43 0.486372518198408 1102 44-45 0.5264158793136602 1102 46-47 0.5695844118598714 1102 48-49 0.5832173680826571 1102 50-51 0.47349665400813024 1102 52-53 0.5502480636903471 1102 54-55 0.632291959334708 1102 56-57 0.6385343551624061 1102 58-59 0.5390899177504522 1102 60-61 0.580681540884953 1102 62-63 0.5881825965268135 1102 64-65 0.5287395097748622 1102 66-67 0.5414730576512667 1102 68-69 0.515497250191622 1102 70-71 0.5492840739997966 1102 72-73 0.47966962826989956 1102 74-75 0.451797411784959 1102 76-77 0.47400191270616787 1102 78-79 0.48589863774970254 1102 80-81 0.49842510479658486 1102 82-83 0.5336353476886515 1102 84-85 0.5332119693642703 1102 86-87 0.4952526606650096 1102 88-89 0.48704857853185857 1102 90-91 0.47863963328491366 1102 92-93 0.4661978855725941 1102 94-95 0.5372929926144714 1102 96-97 0.5420302561397214 1102 98-99 0.43557399819763987 1102 100 0.4337147386920428 1103 1 0.3151508305779416 1103 2 0.3578352521428485 1103 3 0.36095685175560277 1103 4 0.29356119790406154 1103 5 0.3181829834232701 1103 6 0.2896488128399568 1103 7 0.28523143995923306 1103 8 0.3314747539734313 1103 9 0.3931917197781303 1103 10-11 0.36257128232993097 1103 12-13 0.381700163928393 1103 14-15 0.47070584982731134 1103 16-17 0.41655380895051763 1103 18-19 0.3906352726548903 1103 20-21 0.3985847041617063 1103 22-23 0.44166990039903453 1103 24-25 0.4957512924161307 1103 26-27 0.47168598474934953 1103 28-29 0.47408493387943906 1103 30-31 0.45104560999164534 1103 32-33 0.44167308660064464 1103 34-35 0.48351576900506643 1103 36-37 0.4750697723505084 1103 38-39 0.4453225875258653 1103 40-41 0.45136209603792565 1103 42-43 0.4991892885835867 1103 44-45 0.5182492754464079 1103 46-47 0.5750176044720448 1103 48-49 0.5644627698502234 1103 50-51 0.4763856929567609 1103 52-53 0.5045653237585839 1103 54-55 0.5779552383586051 1103 56-57 0.5749864856868783 1103 58-59 0.4770216539255898 1103 60-61 0.43795568489467485 1103 62-63 0.366101537276478 1103 64-65 0.3861183630049574 1103 66-67 0.36891510300999286 1103 68-69 0.40820911590019193 1103 70-71 0.36375680107046904 1103 72-73 0.34215527733652706 1103 74-75 0.39602971850725055 1103 76-77 0.3510827531851639 1103 78-79 0.3407998427283552 1103 80-81 0.3431344902568405 1103 82-83 0.4136417353917601 1103 84-85 0.39295534255441567 1103 86-87 0.33377248475869337 1103 88-89 0.31560977562752157 1103 90-91 0.3571227587249304 1103 92-93 0.290526366332589 1103 94-95 0.3518666911651316 1103 96-97 0.3259518797813321 1103 98-99 0.284434986632661 1103 100 0.3061990105319481 1104 1 0.26088910398941323 1104 2 0.3126511433716601 1104 3 0.27802938361282514 1104 4 0.2370845098973433 1104 5 0.2428563045853096 1104 6 0.20616405582229902 1104 7 0.23811290682680664 1104 8 0.2583728623561683 1104 9 0.3042801638988024 1104 10-11 0.283255335582389 1104 12-13 0.2909963866429095 1104 14-15 0.3737221508630455 1104 16-17 0.32744580820607183 1104 18-19 0.2952727158527182 1104 20-21 0.2208489105224487 1104 22-23 0.3106850920546691 1104 24-25 0.3274857927691528 1104 26-27 0.3252634926648241 1104 28-29 0.32141399296608597 1104 30-31 0.29841047065976056 1104 32-33 0.29614326460642815 1104 34-35 0.2804027172602943 1104 36-37 0.25835095921434004 1104 38-39 0.2538337313316461 1104 40-41 0.2116874444491188 1104 42-43 0.24444745131874157 1104 44-45 0.2777642685916817 1104 46-47 0.306838548384782 1104 48-49 0.2783678408241954 1104 50-51 0.23654631454588326 1104 52-53 0.23719096993701783 1104 54-55 0.3529413598080282 1104 56-57 0.35422043850790885 1104 58-59 0.3367901936787945 1104 60-61 0.3924765636346308 1104 62-63 0.28247471741406827 1104 64-65 0.28812868423311144 1104 66-67 0.31389240440056554 1104 68-69 0.29798449382890624 1104 70-71 0.2696909722387737 1104 72-73 0.22934269526534123 1104 74-75 0.24468338413213786 1104 76-77 0.2768104464581391 1104 78-79 0.25518111145843747 1104 80-81 0.2770318513204053 1104 82-83 0.312858071145488 1104 84-85 0.2810403491244031 1104 86-87 0.1840862387003277 1104 88-89 0.20526953088842959 1104 90-91 0.1650777221094124 1104 92-93 0.20233014266102956 1104 94-95 0.25970696067776444 1104 96-97 0.23625654214383118 1104 98-99 0.25286243123351326 1104 100 0.21865825127605376 1105 1 0.18805659927217278 1105 2 0.23235668311391322 1105 3 0.253873729754055 1105 4 0.20474095989203533 1105 5 0.18579889705497976 1105 6 0.18936819874186028 1105 7 0.18681784519425548 1105 8 0.1964638768729685 1105 9 0.20479023318684852 1105 10-11 0.19958432891274924 1105 12-13 0.212667640234109 1105 14-15 0.2605266750088049 1105 16-17 0.18539729984899367 1105 18-19 0.20536069137673252 1105 20-21 0.22362672794338323 1105 22-23 0.2615427076491841 1105 24-25 0.31837153110843275 1105 26-27 0.3495908782425232 1105 28-29 0.32594466046960235 1105 30-31 0.33323322417533063 1105 32-33 0.34151844301496226 1105 34-35 0.34659375570928574 1105 36-37 0.34382328393801487 1105 38-39 0.34420363755952366 1105 40-41 0.3359894425381924 1105 42-43 0.36114523094189366 1105 44-45 0.35300971894533006 1105 46-47 0.369962608700817 1105 48-49 0.44845975608445343 1105 50-51 0.2692998735870944 1105 52-53 0.3429842440987585 1105 54-55 0.49468372234439073 1105 56-57 0.4614893811652365 1105 58-59 0.40961937080339084 1105 60-61 0.3881901614468788 1105 62-63 0.38415619834875514 1105 64-65 0.36335789276551367 1105 66-67 0.3379851384029102 1105 68-69 0.32204803529464243 1105 70-71 0.3285925666404026 1105 72-73 0.30666858114332385 1105 74-75 0.2611400039841669 1105 76-77 0.29151092929162203 1105 78-79 0.35962861729275275 1105 80-81 0.2778070865836533 1105 82-83 0.25838178507497744 1105 84-85 0.2927472505942923 1105 86-87 0.24244389158952728 1105 88-89 0.24064501818346073 1105 90-91 0.2682067683935223 1105 92-93 0.25692702269056866 1105 94-95 0.32435252415913496 1105 96-97 0.22611663866090836 1105 98-99 0.24146356455610984 1105 100 0.20804027009753057 1106 1 0.2601097114412667 1106 2 0.31065735454462384 1106 3 0.26829817827252356 1106 4 0.269790937985519 1106 5 0.27091197940693235 1106 6 0.2338908755251481 1106 7 0.21591990458254173 1106 8 0.2249409834660696 1106 9 0.2773617684145435 1106 10-11 0.23543953255610717 1106 12-13 0.2500088124485629 1106 14-15 0.3249974810581904 1106 16-17 0.29852780251837885 1106 18-19 0.27243622838800263 1106 20-21 0.29101370962705175 1106 22-23 0.31128460858459306 1106 24-25 0.3376488630102088 1106 26-27 0.32562658409548106 1106 28-29 0.321166326242448 1106 30-31 0.31141814319374816 1106 32-33 0.326125454002522 1106 34-35 0.3026343080599574 1106 36-37 0.28560444736453405 1106 38-39 0.27544888800964884 1106 40-41 0.3277817852439284 1106 42-43 0.31610317963083645 1106 44-45 0.30035594235873475 1106 46-47 0.3180157766043479 1106 48-49 0.3300991086402476 1106 50-51 0.17393347523698566 1106 52-53 0.2591920272112844 1106 54-55 0.3382465059799884 1106 56-57 0.3486729977248686 1106 58-59 0.2887381761456709 1106 60-61 0.29329382270699256 1106 62-63 0.2688408633374735 1106 64-65 0.173184769760109 1106 66-67 0.18790375442990026 1106 68-69 0.16525363701907025 1106 70-71 0.23669162011133338 1106 72-73 0.1888820283401813 1106 74-75 0.19840523174775626 1106 76-77 0.17495941924092406 1106 78-79 0.18769466696711845 1106 80-81 0.12192385927257376 1106 82-83 0.18337693127686805 1106 84-85 0.15016957500619554 1106 86-87 0.1050072727071445 1106 88-89 0.1389975077465273 1106 90-91 0.101822179780509 1106 92-93 0.11033774424542742 1106 94-95 0.1665979861661171 1106 96-97 0.15150911993304206 1106 98-99 0.0739873487953524 1106 100 0.058265271765744586 1107 1 0.22076102503570638 1107 2 0.22060830316990376 1107 3 0.22681500105976227 1107 4 0.24231765627192914 1107 5 0.23664971994267603 1107 6 0.21218877769328515 1107 7 0.22416191953384157 1107 8 0.2116352978734497 1107 9 0.2403134109189793 1107 10-11 0.22686683508500494 1107 12-13 0.2417262264699005 1107 14-15 0.2782867153319728 1107 16-17 0.25781402458137137 1107 18-19 0.23097509578134634 1107 20-21 0.24063076012947704 1107 22-23 0.22047022219855705 1107 24-25 0.28110282353294735 1107 26-27 0.2786309796780344 1107 28-29 0.27263267525193413 1107 30-31 0.2447745953881082 1107 32-33 0.22222963721384303 1107 34-35 0.2302803075838753 1107 36-37 0.23484336461226007 1107 38-39 0.20240483988769142 1107 40-41 0.18665317929468728 1107 42-43 0.20682770906913817 1107 44-45 0.2349084073127372 1107 46-47 0.21724236230084415 1107 48-49 0.23825021924526624 1107 50-51 0.16120979410198544 1107 52-53 0.16231579489542014 1107 54-55 0.24454594576499744 1107 56-57 0.2095758883926635 1107 58-59 0.11017935983851856 1107 60-61 0.15850230470154258 1107 62-63 0.09934076428222482 1107 64-65 0.09719687374834507 1107 66-67 0.12670513033559772 1107 68-69 0.10046735063506418 1107 70-71 0.10928115993007026 1107 72-73 0.1417610136133085 1107 74-75 0.16505153680768814 1107 76-77 0.15143862573895106 1107 78-79 0.12300408774408922 1107 80-81 0.044901912334143645 1107 82-83 0.07750312024206707 1107 84-85 0.10692888094083486 1107 86-87 0.1064195285055014 1107 88-89 0.07858830883005652 1107 90-91 0.07695947627298594 1107 92-93 0.08114875657581422 1107 94-95 0.051501159541995634 1107 96-97 0.08357664079350258 1107 98-99 0.05986542193653577 1107 100 7.328245673861034E-4 1108 1 0.12392714155478757 1108 2 0.15727887025911258 1108 3 0.15270203881468092 1108 4 0.15271358049450612 1108 5 0.178033083730341 1108 6 0.10270583684432211 1108 7 0.12407905669333275 1108 8 0.14143505330372363 1108 9 0.16356907103291718 1108 10-11 0.13426266192136183 1108 12-13 0.1591439458079904 1108 14-15 0.2040513687499299 1108 16-17 0.15322139889669728 1108 18-19 0.133601226105597 1108 20-21 0.10340475834708229 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-1.7361724139669512 2316 92-93 -1.7007236341115437 2316 94-95 -1.904637192900612 2316 96-97 -2.1170795592228906 2316 98-99 -1.9869906588801598 2316 100 -1.739071775385323 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12488.0 3 3161.0 4 2135.0 5 1802.0 6 3217.0 7 21906.0 8 41198.0 9 43337.0 10 31619.0 11 31510.0 12 37058.0 13 38622.0 14 48050.0 15 69092.0 16 112983.0 17 160232.0 18 179576.0 19 196697.0 20 222965.0 21 262182.0 22 345435.0 23 651090.0 24 866553.0 25 500103.0 26 396310.0 27 390359.0 28 412969.0 29 456495.0 30 539722.0 31 657491.0 32 834039.0 33 1118667.0 34 1604289.0 35 2480980.0 36 3858681.0 37 4616100.0 38 2535977.0 39 111378.0 40 218.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 16.36560862126381 36.70322974718182 22.64339677673152 24.28776485482284 2 24.773468367149547 34.86572266496733 22.211838954993183 18.148970012889933 3 31.059168326838726 23.40737183043438 24.007846427192497 21.525613415534398 4 21.445802375359047 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20.06843834679443 30.871824493066057 23.388757894688343 25.670979265451173 26-27 21.397163413756314 24.41885030648087 32.91617363805046 21.26781264171235 28-29 23.601592748015605 24.118700312626196 33.086232130211776 19.193474809146426 30-31 21.482036616380505 25.424012671206576 33.39306630460347 19.70088440780945 32-33 20.624243589304815 23.963880458551344 33.6329408435154 21.778935108628442 34-35 20.802669036894844 26.449334403834634 32.71622209109522 20.031774468175307 36-37 22.324499315020738 24.428244063109872 31.850030875328493 21.397225746540897 38-39 20.32298354622951 24.74115129897064 32.76736277472973 22.168502380070127 40-41 21.63734804613602 24.83543646085113 32.52420291060751 21.003012582405344 42-43 22.541189609969354 24.381014800237907 33.527960210887855 19.549835378904884 44-45 20.867245684142596 25.891444786167966 32.39003906139179 20.851270468297646 46-47 20.07198981248975 24.887745355801645 32.14934729194793 22.89091753976067 48-49 22.780691371327322 24.615018676273454 32.33231609853286 20.271973853866374 50-51 22.855232698330845 24.37428498261967 31.783636387360282 20.986845931689206 52-53 21.565000986201536 24.310591507071567 33.055822902710005 21.068584604016888 54-55 22.674915210130834 24.454813352532906 32.34998575280527 20.520285684530997 56-57 21.107086621594945 26.96005282513404 30.655832836308587 21.27702771696243 58-59 20.66464271154132 24.658407303263 30.461888890359734 24.21506109483595 60-61 22.37309036754752 25.777928666868284 27.582397979108315 24.266582986475886 62-63 23.864383496137112 25.678536316203576 27.891829316317963 22.565250871341345 64-65 22.59810210969103 24.973607129039237 28.91097855033289 23.517312210936844 66-67 22.097758306670652 27.116032685667214 28.461984697508086 22.324224310154055 68-69 23.71951290539086 25.66259131536445 28.53988897472957 22.078006804515123 70-71 21.18599046376367 27.77165382935025 26.908249728099065 24.134105978787012 72-73 22.529081629489582 25.352483208182676 27.703266412963494 24.41516874936425 74-75 24.612424571270346 24.823243886831598 28.410781389240743 22.153550152657314 76-77 22.853480917109557 25.811881210103067 29.386061802542667 21.94857607024471 78-79 22.311329700653975 24.66481811054897 29.70390704136997 23.319945147427077 80-81 22.648017925413065 26.848029340872426 28.60350915409875 21.90044357961576 82-83 24.07706661106664 25.31106919325043 27.4753521010228 23.13651209466013 84-85 22.790624240713008 25.712780056777778 27.843646627910573 23.652949074598638 86-87 24.078554988450012 25.96372512500089 27.557512141836256 22.400207744712837 88-89 24.511263585719323 25.764826650355303 28.621115618935804 21.102794144989574 90-91 22.910397710328915 27.402258760390662 27.371748279670705 22.315595249609718 92-93 22.263020070063053 26.7239765178058 26.591010600479265 24.421992811651887 94-95 24.50977212496232 26.07307076050783 26.734864249713446 22.682292864816404 96-97 24.739786455759685 25.56853485927306 26.60333012831351 23.08834855665374 98-99 23.85163765711689 25.404615515566388 28.326068262663096 22.417678564653627 100 24.591974835803477 26.947443914594803 28.57134118676832 19.8892400628334 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 11944.0 1 8078.5 2 3598.0 3 2722.0 4 2496.0 5 2540.0 6 2684.0 7 2966.0 8 2983.5 9 3040.0 10 3532.5 11 4623.5 12 23071.5 13 29772.0 14 19577.0 15 22694.0 16 27813.0 17 33512.0 18 39901.5 19 47732.5 20 58120.0 21 72101.5 22 90357.5 23 113147.5 24 138834.5 25 166513.5 26 194500.5 27 220527.5 28 243974.5 29 261694.0 30 270278.5 31 269512.5 32 261169.0 33 247415.5 34 231244.0 35 207683.5 36 178668.5 37 154407.5 38 136012.0 39 126521.5 40 124682.0 41 121545.0 42 119194.0 43 125320.5 44 149037.5 45 234416.0 46 575156.5 47 2564798.0 48 4987733.0 49 5168234.0 50 3324008.0 51 1347506.0 52 472858.5 53 187351.5 54 95080.0 55 64017.0 56 51595.5 57 44056.5 58 37787.5 59 32038.5 60 26622.0 61 21714.0 62 17290.5 63 13449.5 64 10447.0 65 8120.5 66 6292.0 67 5025.0 68 4149.0 69 3557.0 70 3061.5 71 2694.0 72 2377.0 73 2117.0 74 1861.5 75 1647.0 76 1459.5 77 1224.0 78 1007.0 79 845.0 80 700.0 81 561.5 82 460.0 83 362.0 84 279.5 85 219.0 86 183.5 87 147.0 88 99.0 89 71.0 90 55.5 91 43.0 92 31.0 93 20.5 94 17.0 95 15.5 96 11.0 97 9.0 98 12.5 99 12.5 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4001726431857539 2 0.3820236831165627 3 0.16832041062095388 4 0.17477737289597395 5 0.2729625354745842 6 0.102553969198909 7 0.1367762877245824 8 0.14258880917630168 9 0.1353953431032236 10-11 0.13229240238583712 12-13 0.13424664825909335 14-15 0.14004661566880025 16-17 0.14389233720525096 18-19 0.15499429502484152 20-21 0.13598747541813958 22-23 0.09893422041868064 24-25 0.0947558167689026 26-27 0.08912114424569165 28-29 0.09878566425486782 30-31 0.10298708364833517 32-33 0.10419436402185642 34-35 0.10463375549228876 36-37 0.10070643268275777 38-39 0.0998464808049116 40-41 0.10103493011541433 42-43 0.06351508322116298 44-45 0.06672264095531907 46-47 0.08532982355796113 48-49 0.08929899317419997 50-51 0.09866430851541506 52-53 0.10374032616907633 54-55 0.10595611458425658 56-57 0.10659846306722195 58-59 0.1057636192734005 60-61 0.10397885296731103 62-63 0.10827652001620643 64-65 0.11529213716077619 66-67 0.1235631585065812 68-69 0.12875425487868902 70-71 0.12745281919007515 72-73 0.1143338452871666 74-75 0.1118125751830191 76-77 0.11388608445539268 78-79 0.1125051398340339 80-81 0.10691231411753077 82-83 0.1096093408098512 84-85 0.11576709841691019 86-87 0.12912250677771805 88-89 0.1370441072875126 90-91 0.14392790699095265 92-93 0.1430323853274048 94-95 0.13292219682679013 96-97 0.1297209161136402 98-99 0.12496084185062313 100 0.1192173676299718 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.3896686E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 11.159673429932115 #Duplication Level Percentage of deduplicated Percentage of total 1 83.28851250970213 9.294726000730915 2 8.014782868506733 1.7888471884869936 3 2.730724385192469 0.9142197709770032 4 1.4067114522672435 0.627937616697919 5 0.8828359611208897 0.4926080509154687 6 0.6139246655704235 0.4110719267007729 7 0.42848463916066154 0.33472240499426986 8 0.3221133738748638 0.28757440478856855 9 0.25156225040615254 0.2526617305648323 >10 1.5071108821871588 3.26210617444104 >50 0.22152173333432768 1.7491790191752712 >100 0.22248778813835005 5.502505934337325 >500 0.052518355403504226 4.231015878996948 >1k 0.04825184733888076 9.55398919562542 >5k 0.0033225059199952804 2.699191604230111 >10k+ 0.005134781876356342 58.59764309833723 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1728117 7.231617806753622 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 435967 1.8243826779997863 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 393227 1.6455294261304685 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 341587 1.429432516291171 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 334967 1.4017299302505795 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 328054 1.3728012327734482 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 313994 1.3139646225422221 No Hit AGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCA 287388 1.202627008615337 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 285173 1.1933579409295498 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 278582 1.165776710628411 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 276693 1.1578718488412996 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 270592 1.1323411120688451 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 264154 1.1054001379103362 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 257998 1.0796392437009885 No Hit TGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCG 256308 1.0725671333673632 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 256242 1.0722909444430913 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 255276 1.0682485429151138 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 253463 1.060661716858982 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 247631 1.0362566591869684 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 247459 1.0355368941115934 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 246377 1.031009069625805 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 245996 1.0294147062902361 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 244229 1.0220203755449604 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 242390 1.0143247477913884 No Hit TCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTG 238229 0.9969122915202552 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 229957 0.962296613011528 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 229692 0.9611876726337701 No Hit TGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGT 223354 0.9346651665423398 No Hit TAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG 218783 0.9155369911961851 No Hit CTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA 218757 0.9154281894987447 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 216838 0.9073977872915098 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 213672 0.8941490882878069 No Hit ACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 204808 0.8570560788219757 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 195797 0.8193479212975389 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 194480 0.8138366968541162 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 190683 0.7979474643471485 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 182668 0.7644072487708128 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 180997 0.7574146473699324 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 180691 0.7561341350846724 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 178186 0.7456515100043579 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 177639 0.743362489677439 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 152768 0.6392852967143645 No Hit TACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACT 152331 0.6374565912612318 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 138138 0.5780634185007912 No Hit TTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC 136058 0.5693592827055601 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 130508 0.5461343049827077 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 126802 0.5306258784167813 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 58148 0.2433308116447611 No Hit AAGCAGTGGTATCAACGCAGAGTACGCAGTGGTATCAACGCAGAGTAAGC 32827 0.13737051237983375 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGT 27075 0.1133002291614829 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.4646382347744788E-4 4.184680670784225E-6 0.0 0.0 0.0 2 1.506485041482321E-4 1.2554042012352675E-5 0.0 0.0 0.0 3 1.632025461605848E-4 1.2554042012352675E-5 0.0 0.0 0.0 4 1.67387226831369E-4 1.67387226831369E-5 0.0 0.0 0.0 5 1.7575658817293744E-4 1.67387226831369E-5 0.0 0.0 0.0 6 1.7575658817293744E-4 1.67387226831369E-5 0.0 0.0 0.0 7 1.7575658817293744E-4 1.67387226831369E-5 0.0 0.0 0.0 8 1.7994126884372168E-4 1.67387226831369E-5 0.0 0.0 0.0 9 1.8831063018529012E-4 1.67387226831369E-5 0.0 0.0 0.0 10-11 2.1551105454538757E-4 1.67387226831369E-5 0.0 0.0 0.0 12-13 2.78281264607151E-4 1.67387226831369E-5 0.0 0.0 0.0 14-15 3.682518990290118E-4 1.67387226831369E-5 0.0 0.0 0.0 16-17 4.916999788171464E-4 2.0923403353921125E-5 0.0 0.0 0.0 18-19 6.779182686670445E-4 2.510808402470535E-5 0.0 0.0 0.0 20-21 9.394608105910585E-4 2.510808402470535E-5 0.0 0.0 0.0 22-23 0.0014269761087374207 2.510808402470535E-5 0.0 0.0 0.0 24-25 0.002123725440422994 2.510808402470535E-5 0.0 0.0 0.0 26-27 0.0029292764695489576 2.510808402470535E-5 0.0 0.0 0.0 28-29 0.003998462380934327 2.510808402470535E-5 0.0 0.0 0.0 30-31 0.005588641035832332 2.510808402470535E-5 0.0 0.0 0.0 32-33 0.009036817908558535 2.510808402470535E-5 0.0 0.0 0.0 34-35 0.01925580810661361 2.9292764695489575E-5 0.0 0.0 0.0 36-37 0.04467983552196317 2.9292764695489575E-5 0.0 0.0 0.0 38-39 0.08749330346475659 2.9292764695489575E-5 0.0 0.0 0.0 40-41 0.1466960732546764 2.9292764695489575E-5 0.0 0.0 0.0 42-43 0.21647353109966797 2.9292764695489575E-5 0.0 0.0 0.0 44-45 0.2969533097601902 3.34774453662738E-5 0.0 0.0 0.0 46-47 0.3920334392810786 3.34774453662738E-5 0.0 0.0 0.0 48-49 0.5002304503645401 3.34774453662738E-5 0.0 4.184680670784225E-6 0.0 50-51 0.6210359043090745 3.34774453662738E-5 0.0 4.184680670784225E-6 0.0 52-53 0.7555817572361289 3.766212603705802E-5 0.0 4.184680670784225E-6 0.0 54-55 0.9012630454281401 4.184680670784225E-5 0.0 4.184680670784225E-6 0.0 56-57 1.0646015937105253 4.184680670784225E-5 0.0 4.184680670784225E-6 0.0 58-59 1.246394583751069 4.393914704323436E-5 0.0 8.36936134156845E-6 0.0 60-61 1.4419426191564804 4.603148737862648E-5 0.0 8.36936134156845E-6 0.0 62-63 1.6503334395405287 4.603148737862648E-5 0.0 8.36936134156845E-6 0.0 64-65 1.875138669855728 4.603148737862648E-5 0.0 8.36936134156845E-6 0.0 66-67 2.113383002145151 4.603148737862648E-5 0.0 8.36936134156845E-6 0.0 68-69 2.3688724034788757 4.603148737862648E-5 0.0 1.2554042012352675E-5 0.0 70-71 2.640453994332101 4.603148737862648E-5 0.0 1.2554042012352675E-5 0.0 72-73 2.9240581727524897 4.603148737862648E-5 0.0 1.4646382347744787E-5 2.0923403353921125E-6 74-75 3.2223045488399524 4.603148737862648E-5 0.0 1.883106301852901E-5 4.184680670784225E-6 76-77 3.532768100145769 5.02161680494107E-5 0.0 2.0923403353921125E-5 4.184680670784225E-6 78-79 3.857915695925368 5.02161680494107E-5 0.0 2.0923403353921125E-5 4.184680670784225E-6 80-81 4.2004087930853675 5.02161680494107E-5 0.0 2.0923403353921125E-5 4.184680670784225E-6 82-83 4.556121296484374 5.649318905558704E-5 0.0 2.0923403353921125E-5 4.184680670784225E-6 84-85 4.919156991057253 6.486255039715548E-5 0.0 2.0923403353921125E-5 4.184680670784225E-6 86-87 5.296152780347869 7.323191173872394E-5 0.0 2.0923403353921125E-5 4.184680670784225E-6 88 5.585125904068874 8.36936134156845E-5 0.0 2.0923403353921125E-5 4.184680670784225E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATCTA 475 0.0 37.59992 8 AGTACTT 258535 0.0 37.189987 20-21 TCCGATC 1265 0.0 36.785995 6 TACTTTT 264105 0.0 36.317917 22-23 GAGTACT 257320 0.0 35.870487 20-21 GTACTTT 259070 0.0 35.460274 22-23 CCGATCT 1335 0.0 34.854145 7 ACTTTTT 286765 0.0 32.468582 24-25 TTCCGAT 1535 0.0 30.367426 5 AGAGTAC 336925 0.0 29.798523 18-19 CGATCTT 540 0.0 26.111052 8 CTTCCGA 1870 0.0 24.683098 4 CTTTTTT 469650 0.0 20.766092 24-25 GTACCTT 1970 0.0 20.393944 22-23 GAGTACC 2135 0.0 19.263483 20-21 GTACTTC 1535 0.0 18.979494 22-23 GAACTTT 3015 0.0 18.234753 22-23 AGTACCT 2310 0.0 18.21108 20-21 GAGAACT 2975 0.0 17.9322 20-21 CGATCTC 715 0.0 17.089619 9 >>END_MODULE