##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660709_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 23896686 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.120308146493617 33.0 31.0 34.0 27.0 34.0 2 31.814470048273638 34.0 31.0 34.0 30.0 34.0 3 31.51658246670689 34.0 31.0 34.0 28.0 34.0 4 35.42245016735794 37.0 35.0 37.0 33.0 37.0 5 35.79159152026352 37.0 35.0 37.0 33.0 37.0 6 35.220522962891174 37.0 35.0 37.0 33.0 37.0 7 35.466150787602935 37.0 35.0 37.0 33.0 37.0 8 35.85661790090894 37.0 35.0 37.0 33.0 37.0 9 37.67559510134585 39.0 37.0 39.0 35.0 39.0 10-11 37.72831201364072 39.0 37.0 39.0 35.0 39.0 12-13 37.74025971634728 39.0 37.0 39.0 35.0 39.0 14-15 39.129810531050204 40.0 38.5 41.0 36.0 41.0 16-17 39.07088076982725 40.0 38.0 41.0 36.0 41.0 18-19 39.058558831128295 40.0 38.5 41.0 36.0 41.0 20-21 38.925409175983646 40.0 38.5 41.0 35.0 41.0 22-23 38.79733321599488 40.0 38.5 41.0 34.5 41.0 24-25 38.830916638399145 40.0 38.0 41.0 35.0 41.0 26-27 38.63103308550818 40.0 38.0 41.0 35.0 41.0 28-29 38.46160423667114 40.0 38.0 41.0 34.0 41.0 30-31 38.25931581056888 40.0 38.0 41.0 34.0 41.0 32-33 38.105035966074965 40.0 37.5 41.0 33.5 41.0 34-35 37.97788626422928 40.0 37.5 41.0 33.0 41.0 36-37 37.7395916948484 39.5 37.0 41.0 33.0 41.0 38-39 37.19773911328123 39.0 35.0 41.0 32.5 41.0 40-41 37.00577969263186 39.0 35.0 41.0 31.5 41.0 42-43 36.6605019834131 39.0 35.0 40.0 30.5 41.0 44-45 36.4670283151396 39.0 35.0 40.0 30.0 41.0 46-47 36.179539790580165 38.0 35.0 40.0 29.5 41.0 48-49 36.34282958733274 39.0 35.0 40.0 30.0 41.0 50-51 36.36202465479941 39.0 35.0 40.5 29.5 41.0 52-53 35.991884188460276 39.0 35.0 40.0 28.0 41.0 54-55 35.650701607745944 39.0 35.0 40.0 27.0 41.0 56-57 35.10014051739225 39.0 34.0 40.0 22.5 41.0 58-59 34.69363611757714 39.0 34.0 40.0 21.0 41.0 60-61 34.31520027505069 38.5 34.0 40.0 18.5 41.0 62-63 33.69733803256234 37.0 33.0 39.5 14.5 41.0 64-65 33.115767182110524 37.0 32.5 39.0 13.0 40.5 66-67 32.47812050172982 36.0 32.0 39.0 11.5 39.0 68-69 31.934043343918063 35.5 31.0 37.5 10.0 39.0 70-71 31.410679895111816 35.0 31.0 37.0 10.0 38.0 72-73 30.869915686216906 35.0 31.0 36.0 10.0 37.0 74-75 30.45114159344103 34.0 30.0 35.0 10.0 37.0 76-77 28.761168849940113 32.5 27.5 34.0 10.0 35.5 78-79 29.834658014923072 34.0 29.5 35.0 10.0 36.0 80-81 29.963032907575553 34.0 30.0 35.0 10.0 36.0 82-83 29.784060308613505 34.0 30.0 35.0 10.0 35.0 84-85 29.612736385287903 34.0 29.0 35.0 10.0 35.0 86-87 29.53597854530959 34.0 29.0 35.0 10.0 35.0 88-89 29.38360057541033 34.0 29.0 35.0 10.0 35.0 90-91 29.27902655623462 34.0 29.0 35.0 9.0 35.0 92-93 29.14119275785772 34.0 29.0 35.0 8.0 35.0 94-95 29.103843541317822 34.0 29.0 35.0 8.0 35.0 96-97 28.952146272499874 34.0 29.0 35.0 8.0 35.0 98-99 28.711228326806488 34.0 27.0 35.0 8.0 35.0 100 28.69361408523341 34.0 27.0 35.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.5330645915569825 1101 2 1.1383787337073485 1101 3 1.5345819405943502 1101 4 0.9542984442867919 1101 5 0.5679638987904525 1101 6 1.1212917950230263 1101 7 0.8211891374380897 1101 8 0.49134446718338864 1101 9 0.5254946064962382 1101 10-11 0.3970968505604091 1101 12-13 0.38723418997674486 1101 14-15 0.4878754487035195 1101 16-17 0.5271306768082411 1101 18-19 0.5018770401962556 1101 20-21 0.5065090031521038 1101 22-23 0.47214210834567183 1101 24-25 0.4630514492700186 1101 26-27 0.5175464342831262 1101 28-29 0.5577964664727091 1101 30-31 0.5896745730673274 1101 32-33 0.640674652244364 1101 34-35 0.6291525483251803 1101 36-37 0.574280355349984 1101 38-39 0.5452418358090725 1101 40-41 0.558797721361664 1101 42-43 0.5880578891283577 1101 44-45 0.5233189256449506 1101 46-47 0.5753941590914238 1101 48-49 0.5902755986663593 1101 50-51 0.5987767067610719 1101 52-53 0.6216233522950176 1101 54-55 0.6100416365182895 1101 56-57 0.5572038428125268 1101 58-59 0.47405416030596825 1101 60-61 0.43860005774715916 1101 62-63 0.38768487242199257 1101 64-65 0.3922354717790242 1101 66-67 0.39124005714743504 1101 68-69 0.3000211948541782 1101 70-71 0.3278387944231689 1101 72-73 0.3121797749394055 1101 74-75 0.34877808261474996 1101 76-77 0.30297958911051026 1101 78-79 0.40716108550218166 1101 80-81 0.38903075710485524 1101 82-83 0.4024140859485996 1101 84-85 0.3593161161842069 1101 86-87 0.3819834936758042 1101 88-89 0.3992339553058706 1101 90-91 0.41802661706038435 1101 92-93 0.36311070810521784 1101 94-95 0.36759748707236994 1101 96-97 0.3967025158144999 1101 98-99 0.4766676973700861 1101 100 0.49500944613404485 1102 1 1.4821232070988977 1102 2 1.1139498263785157 1102 3 1.5091547096578637 1102 4 0.9186535002917395 1102 5 0.5236119112253377 1102 6 1.0795613975348743 1102 7 0.7981852444557802 1102 8 0.44129513589466285 1102 9 0.4514740829574748 1102 10-11 0.3641284930016653 1102 12-13 0.3733508445580469 1102 14-15 0.46983208538030397 1102 16-17 0.4897433600418921 1102 18-19 0.4384004228866374 1102 20-21 0.4158325462778407 1102 22-23 0.3964617265868924 1102 24-25 0.4149959874948763 1102 26-27 0.4479130984876605 1102 28-29 0.5080756804622197 1102 30-31 0.5208420198590389 1102 32-33 0.510391925863054 1102 34-35 0.4967877290482434 1102 36-37 0.4697109351647768 1102 38-39 0.4619332487472718 1102 40-41 0.47184422396811243 1102 42-43 0.5187506864666886 1102 44-45 0.4122241331237646 1102 46-47 0.4530912374151086 1102 48-49 0.45722529143573354 1102 50-51 0.4216096881036435 1102 52-53 0.4681790349868251 1102 54-55 0.45905737966175053 1102 56-57 0.5088069398586867 1102 58-59 0.4339925891987022 1102 60-61 0.416247634544483 1102 62-63 0.3724439846591281 1102 64-65 0.3499530894591629 1102 66-67 0.30487963375824023 1102 68-69 0.3124301036485484 1102 70-71 0.23080903729691826 1102 72-73 0.23122849871461426 1102 74-75 0.32392808397744943 1102 76-77 0.1192812197879185 1102 78-79 0.28912017621573227 1102 80-81 0.3450328571327397 1102 82-83 0.36942732911781917 1102 84-85 0.3781696571836761 1102 86-87 0.3779951903028973 1102 88-89 0.3880998421621804 1102 90-91 0.37524700612103246 1102 92-93 0.34763211604979816 1102 94-95 0.3125889055750477 1102 96-97 0.3417182985769003 1102 98-99 0.36935543148918626 1102 100 0.39888514105244255 1103 1 1.4376059866599924 1103 2 1.0610747010525188 1103 3 1.4248448751687661 1103 4 0.8761202680565958 1103 5 0.49639742101511786 1103 6 1.0508054348685079 1103 7 0.7434954693641913 1103 8 0.4244571378979458 1103 9 0.4566505018380269 1103 10-11 0.372393904072716 1103 12-13 0.3552990867802919 1103 14-15 0.3970513953469492 1103 16-17 0.41163412069970917 1103 18-19 0.3716202722206887 1103 20-21 0.37602352254637594 1103 22-23 0.362569995102902 1103 24-25 0.36393358813341337 1103 26-27 0.4216624973059311 1103 28-29 0.41961457380425315 1103 30-31 0.43109453725404734 1103 32-33 0.4613720722312067 1103 34-35 0.5221664980119485 1103 36-37 0.4554726906353963 1103 38-39 0.4412746404340382 1103 40-41 0.4599904087866733 1103 42-43 0.48905312205596374 1103 44-45 0.4956305416010025 1103 46-47 0.4771512236611031 1103 48-49 0.4739922149928333 1103 50-51 0.4617347608559186 1103 52-53 0.4649240612445382 1103 54-55 0.47596173780893736 1103 56-57 0.43075090655513293 1103 58-59 0.39548107895290485 1103 60-61 0.3832295479971535 1103 62-63 0.3363222698967263 1103 64-65 0.33368697184531726 1103 66-67 0.3303395966481162 1103 68-69 0.31295415145234884 1103 70-71 0.28944809887340384 1103 72-73 0.23233907879478366 1103 74-75 0.2970565271754708 1103 76-77 0.18436838767790675 1103 78-79 0.333569193268378 1103 80-81 0.3661940743423706 1103 82-83 0.3671335662190458 1103 84-85 0.3223551701306384 1103 86-87 0.2807638273808095 1103 88-89 0.3336407584054406 1103 90-91 0.31331564100512566 1103 92-93 0.2856765422900729 1103 94-95 0.2898786720700812 1103 96-97 0.2937098955603439 1103 98-99 0.330777602596239 1103 100 0.33224526551279965 1104 1 1.343570371945031 1104 2 0.9766791251359237 1104 3 1.3809405523162184 1104 4 0.8555257068936442 1104 5 0.4682328993417002 1104 6 1.0083092887220317 1104 7 0.7327302518870127 1104 8 0.39922518438365984 1104 9 0.35482416921384896 1104 10-11 0.29575800032593946 1104 12-13 0.29573737022660396 1104 14-15 0.35077045199261647 1104 16-17 0.3496091830253576 1104 18-19 0.3156480029042612 1104 20-21 0.3061285125343076 1104 22-23 0.271659061290876 1104 24-25 0.30195556708417115 1104 26-27 0.31748090535711526 1104 28-29 0.3400735804736996 1104 30-31 0.34415770214751973 1104 32-33 0.3553834546369856 1104 34-35 0.3861622977751509 1104 36-37 0.3632156105802906 1104 38-39 0.3742702818612642 1104 40-41 0.3293903592531464 1104 42-43 0.362244833440144 1104 44-45 0.31883366689319104 1104 46-47 0.30865778031086677 1104 48-49 0.29924135914181704 1104 50-51 0.2597916824213726 1104 52-53 0.26006563579345965 1104 54-55 0.2712574484201653 1104 56-57 0.2857163587917029 1104 58-59 0.22345795785430767 1104 60-61 0.2586009254231456 1104 62-63 0.17314141627290525 1104 64-65 0.12731828574685977 1104 66-67 0.13182173127479047 1104 68-69 0.08319093577904724 1104 70-71 0.09534356908013919 1104 72-73 0.10741051840151528 1104 74-75 0.15529027002308027 1104 76-77 0.04753989232634481 1104 78-79 0.1845315473489677 1104 80-81 0.17145156002820983 1104 82-83 0.2266179373457078 1104 84-85 0.20171001670602706 1104 86-87 0.20530780862084796 1104 88-89 0.22523270304169785 1104 90-91 0.22737966037958657 1104 92-93 0.21262310964155873 1104 94-95 0.22712683743535322 1104 96-97 0.16951227950839254 1104 98-99 0.226497498389854 1104 100 0.2453651852630223 1105 1 1.2703915890968993 1105 2 0.9426957099535755 1105 3 1.3158728328348275 1105 4 0.8254014214125931 1105 5 0.4391891411543938 1105 6 0.9739057837328389 1105 7 0.7051489310531096 1105 8 0.3687670346104497 1105 9 0.33857626823207454 1105 10-11 0.24301074161020608 1105 12-13 0.22682770839351463 1105 14-15 0.30820907112173757 1105 16-17 0.29684359231819 1105 18-19 0.2861723686436761 1105 20-21 0.27594260716801955 1105 22-23 0.2599448551713408 1105 24-25 0.2673035705697586 1105 26-27 0.30983792335581484 1105 28-29 0.3444515873672529 1105 30-31 0.3210858385414568 1105 32-33 0.3528592285480414 1105 34-35 0.351102087373512 1105 36-37 0.32219063823803396 1105 38-39 0.2988954650886555 1105 40-41 0.33197848617115966 1105 42-43 0.3369521822481545 1105 44-45 0.2661716944186594 1105 46-47 0.3007438298968097 1105 48-49 0.28634467754710613 1105 50-51 0.2878674624642841 1105 52-53 0.29000977153847884 1105 54-55 0.30613056304886044 1105 56-57 0.2624782719252039 1105 58-59 0.21776310725284276 1105 60-61 0.17988342612320452 1105 62-63 0.09172674676211301 1105 64-65 0.11745579111133253 1105 66-67 0.11215065484245912 1105 68-69 0.07941179965358103 1105 70-71 0.10354995358995822 1105 72-73 0.07868826904646298 1105 74-75 0.06038169839357721 1105 76-77 -0.05967749140960521 1105 78-79 0.10041108841181057 1105 80-81 0.15540425909103917 1105 82-83 0.1380508503414717 1105 84-85 0.14119013018569504 1105 86-87 0.1464437872436939 1105 88-89 0.13925542134650115 1105 90-91 0.16280724660007095 1105 92-93 0.12994775893106691 1105 94-95 0.11013357342318386 1105 96-97 0.10045316268796611 1105 98-99 0.06439395715471008 1105 100 0.05157912884988036 1106 1 1.3323919654106895 1106 2 0.967450644034308 1106 3 1.3147607084885564 1106 4 0.7972178681972153 1106 5 0.4034802403991975 1106 6 0.9592529458711425 1106 7 0.6823259570449736 1106 8 0.34971875537289066 1106 9 0.3489921829617231 1106 10-11 0.22199346462232228 1106 12-13 0.21216657805260297 1106 14-15 0.2897251774786156 1106 16-17 0.2713718045440814 1106 18-19 0.2176994589578527 1106 20-21 0.2313706725166611 1106 22-23 0.23707345679759584 1106 24-25 0.2270983224263574 1106 26-27 0.21951612072322035 1106 28-29 0.27696382827429034 1106 30-31 0.28434277879589587 1106 32-33 0.2927705752152505 1106 34-35 0.2892724872045491 1106 36-37 0.28186265250628395 1106 38-39 0.2744442111504455 1106 40-41 0.25674565881411127 1106 42-43 0.3007461784989971 1106 44-45 0.28190121536718493 1106 46-47 0.28252769746831774 1106 48-49 0.25274944040062053 1106 50-51 0.26617163518548637 1106 52-53 0.24465358939513493 1106 54-55 0.20045623398377188 1106 56-57 0.13367917037510324 1106 58-59 0.1472403713508399 1106 60-61 0.1154106697554127 1106 62-63 0.09360120773525438 1106 64-65 0.08282824859885807 1106 66-67 0.05572339022666739 1106 68-69 0.07207907143788006 1106 70-71 0.093119081262234 1106 72-73 0.004814254722347755 1106 74-75 0.04047276114798848 1106 76-77 -0.1087153370722369 1106 78-79 0.052167056180635996 1106 80-81 0.10658630433415439 1106 82-83 0.13310700205810377 1106 84-85 0.18080834216027952 1106 86-87 0.1052504422406173 1106 88-89 0.106355814522761 1106 90-91 0.1293220646541684 1106 92-93 0.10869172506703961 1106 94-95 0.10217188541206923 1106 96-97 0.10768573717081509 1106 98-99 0.15605991690750542 1106 100 0.09356237117657429 1107 1 1.2262524284700582 1107 2 0.8476097465587813 1107 3 1.226844393304674 1107 4 0.7750459628816913 1107 5 0.3565963361128013 1107 6 0.9222101655490249 1107 7 0.6372499611189042 1107 8 0.2726724765605866 1107 9 0.24040849228196493 1107 10-11 0.1570446927060729 1107 12-13 0.11565277696539766 1107 14-15 0.15760771719270394 1107 16-17 0.16142410553992903 1107 18-19 0.12850277067091298 1107 20-21 0.12416971352929806 1107 22-23 0.16919924255911667 1107 24-25 0.1464725053771616 1107 26-27 0.1252907064121942 1107 28-29 0.15068054349280402 1107 30-31 0.15231970970867792 1107 32-33 0.1838564974357908 1107 34-35 0.21683308793863887 1107 36-37 0.18634971234487807 1107 38-39 0.20098123046725647 1107 40-41 0.2138884424216556 1107 42-43 0.2064484220714391 1107 44-45 0.18487214790907558 1107 46-47 0.22992048973704726 1107 48-49 0.17541489423348366 1107 50-51 0.17883902088678383 1107 52-53 0.14431842186105115 1107 54-55 0.16168804807895043 1107 56-57 0.11134849913497646 1107 58-59 0.062425958287469996 1107 60-61 0.10504249842464475 1107 62-63 0.09632738024644993 1107 64-65 0.09456631875182353 1107 66-67 0.0633971108987268 1107 68-69 0.03118933054547668 1107 70-71 0.057291677144636566 1107 72-73 0.06471342873669172 1107 74-75 0.06145444125223776 1107 76-77 -0.08813015998176255 1107 78-79 0.04391881950294163 1107 80-81 0.10572892358070263 1107 82-83 0.12442342805138651 1107 84-85 0.098927611849561 1107 86-87 0.07290215590548854 1107 88-89 0.10387232067336427 1107 90-91 0.09957679198007341 1107 92-93 0.06111294793975475 1107 94-95 -0.01203226223245224 1107 96-97 0.03134843527125142 1107 98-99 0.07563277435307114 1107 100 0.04699727889315142 1108 1 1.1713004983677031 1108 2 0.7933365456846317 1108 3 1.1778733001010835 1108 4 0.7241846320049916 1108 5 0.3136069551897407 1108 6 0.8431397075642124 1108 7 0.5849548444658339 1108 8 0.23454053922240803 1108 9 0.17958588411586618 1108 10-11 0.0954438403229858 1108 12-13 0.10310607957681128 1108 14-15 0.1092802086296274 1108 16-17 0.08814025912844414 1108 18-19 0.06048783705617922 1108 20-21 0.06427917305369135 1108 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10050.0 15 26543.0 16 64164.0 17 101125.0 18 119168.0 19 136521.0 20 158705.0 21 191128.0 22 262638.0 23 479881.0 24 691805.0 25 422514.0 26 328064.0 27 323052.0 28 349209.0 29 396593.0 30 472854.0 31 569420.0 32 707136.0 33 923129.0 34 1309842.0 35 2183752.0 36 4542193.0 37 5521780.0 38 3475624.0 39 122264.0 40 269.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 17.468858718236422 35.573763595539134 22.279156167006995 24.678221519217452 2 26.839698592248943 33.9038104088247 22.059379695633496 17.197111303292857 3 29.681236857620114 24.809202160858035 23.806899956637196 21.702661024884655 4 22.565593824533682 27.895367082076262 22.2371774605439 27.30186163284616 5 28.053770823449636 30.839505425796233 21.9207800314663 19.18594371928783 6 30.837473829426727 25.736066532966063 24.247467459389814 19.178992178217403 7 22.568665848613996 26.390560150411652 29.42457272469668 21.616201276277668 8 30.790550824711328 28.20160555031167 23.440209328488685 17.567634296488322 9 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25.19772044670211 31.10791231470604 21.926086388136582 34-35 22.121992145593534 27.67641633225718 30.417318598808972 19.784272923340314 36-37 24.022583352854113 25.6202517819533 29.566389707006273 20.790775158186314 38-39 21.446077472332462 26.14189136906188 30.71168112083064 21.70035003777502 40-41 22.47544631539833 26.549760431519353 30.36997252972083 20.604820723361488 42-43 23.707097174675567 25.785206299643736 31.093071407705363 19.414625117975334 44-45 22.07253511647612 27.33341291101154 29.893712729228245 20.700339243284095 46-47 21.352603421143286 26.11403974214912 29.42990133627022 23.10345550043737 48-49 24.087248786297216 25.891231349031983 30.132526447601798 19.888993417069003 50-51 24.463764906408432 25.7112882889945 29.41482492639615 20.410121878200922 52-53 22.47700077724154 25.973434100327218 30.537337197795118 21.01222792463613 54-55 23.493816883989158 25.803968337523226 30.196401291062834 20.505813487424778 56-57 22.392818197346756 28.403789323866174 28.15135162013473 21.05204085865234 58-59 21.78569488352486 25.970644302046924 28.727703790164195 23.515957024264026 60-61 23.518333945415986 26.68378935531175 25.85907556106431 23.938801138207953 62-63 24.568656532621606 27.316006540084725 26.12028794990813 21.995048977385544 64-65 23.591242447241136 26.226834471399663 27.00957983583435 23.17234324552485 66-67 22.531300721378155 28.507392719365875 26.700922471776895 22.260384087479075 68-69 24.69431955106166 26.716726735441643 26.773223140641196 21.815730572855504 70-71 22.008499996367593 28.948104984516636 25.233261671311435 23.810133347804335 72-73 22.766362796741763 27.04480298719384 26.238407382709422 23.950426833354978 74-75 25.076407270515233 25.943938384453514 26.627799785483493 22.35185455954776 76-77 22.86223618054472 27.705272884451166 27.44840935167411 21.984081583330003 78-79 22.307706688172395 26.141289530886986 27.610414835802477 23.940588945138146 80-81 22.701915931756293 28.331427319450448 26.762065681247478 22.204591067545785 82-83 24.28274345063163 26.722003213734034 25.768927452636714 23.226325882997614 84-85 22.332563457212004 27.271008606102754 26.45054521087579 23.945882725809454 86-87 23.302473109675745 27.74722795820783 26.05753200194265 22.892766930173778 88-89 23.934991846982808 27.2144703084747 26.94245461823483 21.908083226307667 90-91 22.365731443626174 28.771249993719213 25.746513326153696 23.116505236500913 92-93 21.667932550557385 27.894822981193307 24.967648722758153 25.46959574549116 94-95 23.901157650135872 27.439627206492023 25.42983658404922 23.22937855932289 96-97 24.241412662378323 27.07639464245 25.075999701752984 23.606192993418695 98-99 22.648398614803217 27.18560468804917 26.568424706039227 23.59757199110838 100 23.208727317529355 28.743070619728968 26.93931205841692 21.108890004324753 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3120.0 1 2510.5 2 1503.5 3 1088.5 4 1084.5 5 1077.0 6 1285.5 7 1627.5 8 1693.5 9 1799.0 10 2269.0 11 4115.5 12 21322.0 13 25986.5 14 15330.5 15 17627.5 16 21597.0 17 26081.0 18 31038.5 19 36614.5 20 43625.5 21 53018.5 22 65911.5 23 82257.0 24 101672.5 25 123572.5 26 147378.0 27 172169.0 28 196704.0 29 219765.0 30 236123.0 31 244911.5 32 248362.5 33 244595.0 34 234538.0 35 213136.5 36 183216.0 37 154691.0 38 130482.5 39 116084.5 40 110510.5 41 103549.0 42 97123.0 43 99112.0 44 117141.0 45 196528.0 46 569525.0 47 2693572.0 48 5297578.5 49 5489773.0 50 3489626.0 51 1353650.0 52 396138.0 53 120950.0 54 60444.0 55 45913.0 56 39963.0 57 35688.0 58 31259.0 59 26696.5 60 22011.0 61 17730.5 62 13824.0 63 10229.0 64 7490.0 65 5434.5 66 3822.5 67 2738.5 68 1985.5 69 1521.5 70 1165.0 71 911.5 72 789.0 73 632.0 74 491.0 75 430.5 76 368.5 77 257.5 78 167.0 79 124.0 80 99.0 81 85.5 82 59.0 83 45.0 84 33.5 85 23.5 86 19.5 87 12.5 88 7.0 89 4.0 90 4.0 91 2.0 92 1.0 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3249823008931029 2 0.006197512073431437 3 2.4248843542573226 4 7.99274008119787E-4 5 3.766212603705803E-5 6 0.008390284744922372 7 0.007235312879785925 8 3.4314381500430646E-4 9 0.0 10-11 7.950893274490028E-5 12-13 6.277021006176337E-6 14-15 0.0 16-17 2.301574368931324E-5 18-19 0.0 20-21 3.3895913433352223E-4 22-23 3.34774453662738E-5 24-25 4.791459368047938E-4 26-27 0.00365531856593002 28-29 0.006107541439009576 30-31 0.005747658901322133 32-33 0.015364055082784281 34-35 0.009741936601585675 36-37 0.005057186590642736 38-39 0.005293621048542045 40-41 0.0081705890097062 42-43 0.018870817484901464 44-45 0.03380175811825958 46-47 0.033132209210934106 48-49 0.039561971061594065 50-51 0.03983188296485964 52-53 0.061899796482240255 54-55 0.03281208113961911 56-57 0.02195701947960483 58-59 0.01652111928825612 60-61 0.0176405213676909 62-63 0.024538967453478697 64-65 0.044481063190100915 66-67 0.04726178349583704 68-69 0.05013456677633041 70-71 0.0604288812264596 72-73 0.06840906726564512 74-75 0.059179754046230514 76-77 0.059255078298304634 78-79 0.05362459045576445 80-81 0.05104264248189059 82-83 0.04764886645788458 84-85 0.04976003785629522 86-87 0.0579117958029829 88-89 0.06420555553184236 90-91 0.05994973528965481 92-93 0.06478094912407519 94-95 0.0728448287766764 96-97 0.07177355052495563 98-99 0.047165535840409 100 0.007080479694966909 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.3896686E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 9.708534946789186 #Duplication Level Percentage of deduplicated Percentage of total 1 80.34560446808753 7.800381087993292 2 9.262001071883105 1.7984092216715204 3 3.3143929683124767 0.9653369988076206 4 1.6340661276848019 0.6345755242396952 5 1.0358990803637784 0.5028531211529261 6 0.7333000887773415 0.427156172302706 7 0.510363955059233 0.34684204052918777 8 0.389227141077716 0.30230602411134805 9 0.2982267582114432 0.26058104137471005 >10 1.812550809659566 3.420691619190383 >50 0.27206923785690285 1.851408766171129 >100 0.2555487889058227 5.432455737863418 >500 0.06358047046578767 4.41271999640942 >1k 0.061877792698951516 10.886910875453752 >5k 0.005242361872389377 3.4890405952789423 >10k+ 0.006048879083526204 57.468331177450025 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1287960 5.389701316743251 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 500933 2.096244642457954 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 396795 1.6604603667638267 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 382306 1.599828528524834 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 379596 1.5884880439070086 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 358129 1.4986555039472838 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 330408 1.3826519710724743 No Hit AGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCA 327377 1.3699682039593273 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 322738 1.350555470327559 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 313803 1.3131653485341022 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 297986 1.2469762543643081 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 277480 1.1611651925292068 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 269013 1.1257335012896768 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 263397 1.1022323346425527 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 260550 1.0903185487728297 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 254557 1.0652397575128199 No Hit TGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGT 243953 1.0208654036798241 No Hit TAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG 236905 0.9913717743121369 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 221527 0.927019754956817 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 219355 0.9179306285398737 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 215411 0.9014262479743008 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 215037 0.8998611774034274 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 212006 0.8871774102902805 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 208830 0.8738868644798697 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 208592 0.8728909104802232 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 207916 0.870062066346773 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 207249 0.86727088433936 No Hit TGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCG 206129 0.8625840419880815 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 202671 0.8481134162285097 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 200281 0.8381120294253354 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 194465 0.8137739266440543 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 191284 0.8004624574302897 No Hit CTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA 189947 0.7948675393734512 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 188499 0.7888081217621556 No Hit TCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTG 182444 0.7634698803005572 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 180091 0.7536233266822019 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 178834 0.7483631830790262 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 172895 0.7235103645752387 No Hit ACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 165861 0.6940753207369423 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 155737 0.6517096136259228 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 147103 0.6155790807143718 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 142578 0.5966434006790733 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 134646 0.5634505135984127 No Hit TACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACT 119113 0.4984498687391214 No Hit TTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC 108908 0.4557452024937684 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 107717 0.4507612478148643 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 106019 0.44365566003587276 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 53634 0.22444116309684112 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGT 31814 0.13313143086032933 No Hit AAGCAGTGGTATCAACGCAGAGTACGCAGTGGTATCAACGCAGAGTAAGC 29263 0.12245631046915878 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 25697 0.10753373919714224 No Hit AGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 24998 0.10460864740826406 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.4227914280666364E-4 0.0 0.0 0.0 0.0 2 1.506485041482321E-4 0.0 0.0 0.0 0.0 3 1.506485041482321E-4 0.0 0.0 0.0 0.0 4 1.506485041482321E-4 0.0 0.0 0.0 0.0 5 1.5483318481901632E-4 0.0 0.0 0.0 0.0 6 1.5901786548980055E-4 0.0 0.0 0.0 0.0 7 1.5901786548980055E-4 0.0 0.0 0.0 0.0 8 1.5901786548980055E-4 0.0 0.0 0.0 0.0 9 1.7157190750215323E-4 0.0 0.0 0.0 0.0 10-11 1.9877233186225068E-4 0.0 0.0 0.0 0.0 12-13 2.678195629301904E-4 0.0 0.0 0.0 0.0 14-15 3.5988253768744335E-4 0.0 0.0 0.0 0.0 16-17 4.854229578109701E-4 4.184680670784225E-6 0.0 0.0 0.0 18-19 6.862876300086129E-4 6.277021006176337E-6 0.0 0.0 0.0 20-21 9.47830171932627E-4 8.36936134156845E-6 0.0 0.0 0.0 22-23 0.0014604535541036946 8.36936134156845E-6 0.0 0.0 0.0 24-25 0.002209511394174071 8.36936134156845E-6 0.0 0.0 0.0 26-27 0.003048539868666308 1.2554042012352675E-5 0.0 0.0 0.0 28-29 0.004115633439716285 1.2554042012352675E-5 0.0 0.0 0.0 30-31 0.005712089115620467 1.2554042012352675E-5 0.0 0.0 0.0 32-33 0.009204205135389902 1.2554042012352675E-5 0.0 0.0 0.0 34-35 0.019502704266189882 1.2554042012352675E-5 0.0 0.0 0.0 36-37 0.04518827422346346 1.2554042012352675E-5 0.0 0.0 0.0 38-39 0.08834488598126117 1.2554042012352675E-5 0.0 0.0 0.0 40-41 0.14798913958194873 1.2554042012352675E-5 0.0 0.0 0.0 42-43 0.2181348493259693 1.2554042012352675E-5 0.0 0.0 0.0 44-45 0.29869832159990717 1.2554042012352675E-5 0.0 0.0 0.0 46-47 0.3941655340828431 1.2554042012352675E-5 0.0 0.0 0.0 48-49 0.5029860625862514 1.2554042012352675E-5 0.0 0.0 0.0 50-51 0.6247560854254017 1.2554042012352675E-5 0.0 0.0 0.0 52-53 0.7604192480915555 1.883106301852901E-5 0.0 0.0 0.0 54-55 0.9073266477201065 2.720042436009746E-5 0.0 0.0 0.0 56-57 1.0720022014768074 2.9292764695489575E-5 0.0 0.0 0.0 58-59 1.2554878948486832 2.9292764695489575E-5 0.0 0.0 0.0 60-61 1.4527202642240855 2.9292764695489575E-5 0.0 0.0 0.0 62-63 1.6626384930529698 2.9292764695489575E-5 0.0 0.0 0.0 64-65 1.8891531654221845 2.9292764695489575E-5 0.0 0.0 0.0 66-67 2.1291969104000446 2.9292764695489575E-5 0.0 2.0923403353921125E-6 0.0 68-69 2.3875214328882253 2.9292764695489575E-5 0.0 4.184680670784225E-6 0.0 70-71 2.6619590682992613 2.9292764695489575E-5 0.0 4.184680670784225E-6 2.0923403353921125E-6 72-73 2.9483523363867272 3.138510503088169E-5 0.0 4.184680670784225E-6 4.184680670784225E-6 74-75 3.249743499998284 4.184680670784225E-5 0.0 8.36936134156845E-6 4.184680670784225E-6 76-77 3.5634208860592635 4.184680670784225E-5 0.0 8.36936134156845E-6 4.184680670784225E-6 78-79 3.89173000808564 4.184680670784225E-5 0.0 8.36936134156845E-6 4.184680670784225E-6 80-81 4.237627342971322 4.184680670784225E-5 0.0 1.2554042012352675E-5 4.184680670784225E-6 82-83 4.596472079852411 4.184680670784225E-5 0.0 1.67387226831369E-5 4.184680670784225E-6 84-85 4.963052198953445 4.184680670784225E-5 0.0 1.67387226831369E-5 4.184680670784225E-6 86-87 5.344356116994632 4.184680670784225E-5 0.0 2.0923403353921125E-5 4.184680670784225E-6 88 5.636480305260738 4.184680670784225E-5 0.0 2.0923403353921125E-5 4.184680670784225E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATCTA 380 0.0 43.235043 8 TCCGATC 960 0.0 41.079308 6 CCGATCT 1020 0.0 38.66029 7 AGTACTT 220500 0.0 34.720097 20-21 TACTTTT 225345 0.0 33.93264 22-23 GAGTACT 218850 0.0 33.2916 20-21 GTACTTT 221350 0.0 32.806995 22-23 TTCCGAT 1230 0.0 32.443657 5 ACTTTTT 239155 0.0 30.512999 24-25 ACGGAGA 2875 0.0 28.952652 3 AACGGAG 3005 0.0 28.823162 2 GGAGAGT 2795 0.0 27.715206 5 CGGAGAG 3000 0.0 27.073446 4 ATGAACG 2825 0.0 26.590117 4 TGAACGC 2860 0.0 26.26455 5 CTTCCGA 1575 0.0 25.933275 4 AGAGTAC 314350 0.0 25.75537 18-19 CAACGGA 3475 0.0 25.149395 1 TATGAAC 3095 0.0 25.029104 3 GAGAGTA 3045 0.0 24.822966 6 >>END_MODULE