FastQCFastQC Report
Thu 26 May 2016
SRR1660708_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660708_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35706245
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT26125077.316666874380099No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1261660.35334435194739744No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT543870.15231789285039635No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3231250.087.6879358
GTGGTAT3270350.086.7525566
AGTGGTA3273600.086.710155
TGGTATC3269550.086.414797
GTATCAA3294250.085.933139
AAGCAGT3327800.085.712031
GCAGTGG3358100.084.997693
CAGTGGT3394250.083.510324
AGCAGTG3427100.083.311862
CAACGCA3201600.045.42888612-13
ATCAACG3226700.045.1954610-11
ACGCAGA3234300.045.01046414-15
TCAACGC3201800.044.35429812-13
AGAGTAC3237550.044.32000418-19
AACGCAG3222850.044.14198314-15
CGCAGAG3248700.043.7341216-17
GAGTACT3180050.043.72868720-21
TATCAAC3272900.043.19753310-11
AGTACTT3324000.042.8587620-21
GTACTTT3299250.042.10327522-23
Produced by FastQC (version 0.11.5)