FastQCFastQC Report
Thu 26 May 2016
SRR1660708_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660708_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35706245
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24001546.721944578602426No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA927910.25987330787653534No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT605190.16949135928462936No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT523050.14648698007869493No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3123400.086.831718
AAGCAGT3020200.086.671691
GTGGTAT3133100.085.914526
GCAGTGG3138800.085.71363
TGGTATC3169450.085.576817
AGTGGTA3149200.085.5679555
GTATCAA3184200.085.079029
AGCAGTG3193350.083.9399642
CAGTGGT3267050.082.2418064
CAACGCA3081750.045.23153712-13
ATCAACG3101600.045.00943810-11
ACGCAGA3116850.044.84575314-15
AGAGTAC3162350.044.14528318-19
TCAACGC3076950.043.98406212-13
AACGCAG3099000.043.77798514-15
CGCAGAG3131800.043.3308816-17
GAGTACT3113150.043.2827920-21
TATCAAC3157750.042.8757310-11
AGTACTT3260500.042.58573520-21
GTACTTT3232900.041.6651322-23
Produced by FastQC (version 0.11.5)