##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660708_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 35706245 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.261010335867017 34.0 31.0 34.0 27.0 34.0 2 31.906798152536062 34.0 31.0 34.0 30.0 34.0 3 31.659253332295233 34.0 31.0 34.0 30.0 34.0 4 35.58478193380458 37.0 35.0 37.0 33.0 37.0 5 35.92593088407924 37.0 35.0 37.0 35.0 37.0 6 35.39652951465493 37.0 35.0 37.0 33.0 37.0 7 35.595710946362466 37.0 35.0 37.0 35.0 37.0 8 35.97595627879661 37.0 35.0 37.0 35.0 37.0 9 37.79664711313105 39.0 38.0 39.0 35.0 39.0 10-11 37.78287747703518 39.0 37.5 39.0 35.0 39.0 12-13 37.8882487643268 39.0 38.0 39.0 35.0 39.0 14-15 39.35644317961746 41.0 39.0 41.0 36.0 41.0 16-17 39.319090554047335 41.0 39.0 41.0 36.0 41.0 18-19 39.29852345156989 41.0 39.0 41.0 36.0 41.0 20-21 39.2199581473773 40.5 39.0 41.0 36.0 41.0 22-23 39.06502013863401 40.0 38.5 41.0 35.5 41.0 24-25 39.09928177829957 40.0 39.0 41.0 36.0 41.0 26-27 39.085894260233744 40.0 39.0 41.0 35.5 41.0 28-29 39.021311566085984 40.0 39.0 41.0 35.0 41.0 30-31 38.934958464548714 40.0 38.5 41.0 35.0 41.0 32-33 38.82225252193279 40.0 38.0 41.0 35.0 41.0 34-35 38.74338690612805 40.0 38.0 41.0 35.0 41.0 36-37 38.60356007751585 40.0 38.0 41.0 35.0 41.0 38-39 38.131875418431704 40.0 37.0 41.0 34.0 41.0 40-41 38.00314376378698 40.0 36.5 41.0 33.5 41.0 42-43 37.842809640162386 40.0 36.0 41.0 33.0 41.0 44-45 37.72327077518232 40.0 36.0 41.0 33.0 41.0 46-47 37.57929722097633 40.0 35.0 41.0 33.0 41.0 48-49 37.62710915135433 40.0 35.5 41.0 33.0 41.0 50-51 37.6206677991483 40.0 36.0 41.0 33.0 41.0 52-53 37.34275871069613 40.0 35.0 41.0 32.5 41.0 54-55 36.73355733709887 40.0 35.0 41.0 30.5 41.0 56-57 36.145297580297225 39.0 35.0 41.0 27.5 41.0 58-59 35.990290550014436 39.0 35.0 41.0 27.0 41.0 60-61 35.86238230875299 39.0 35.0 41.0 27.0 41.0 62-63 35.64757670541945 39.0 35.0 41.0 27.0 41.0 64-65 35.40245162995997 38.0 35.0 40.5 27.0 41.0 66-67 35.152846567876296 37.5 35.0 40.0 26.0 41.0 68-69 34.9216158265872 37.0 35.0 40.0 26.0 41.0 70-71 34.65258167023724 36.5 34.0 39.5 26.0 41.0 72-73 34.32516635395292 36.0 34.0 39.0 26.0 41.0 74-75 33.94514552006239 35.5 34.0 39.0 26.0 40.5 76-77 32.233706302636975 34.5 31.5 36.5 23.0 39.0 78-79 33.16517861231277 35.0 33.5 37.0 25.0 39.0 80-81 33.057120442096334 35.0 34.0 37.0 25.0 39.0 82-83 32.78489061227245 35.0 34.0 36.0 24.5 38.5 84-85 32.524607180060514 35.0 34.0 36.0 24.0 37.0 86-87 32.30576810863198 35.0 33.5 35.5 24.0 37.0 88-89 32.12715425550908 35.0 33.0 35.0 23.5 36.5 90-91 31.95748404795856 35.0 33.0 35.0 23.0 36.0 92-93 31.804697889682885 35.0 33.0 35.0 21.5 36.0 94-95 31.691519690743174 35.0 33.0 35.0 20.0 36.0 96-97 31.58448099205055 35.0 33.0 35.0 20.0 35.5 98-99 31.488836546660117 35.0 33.0 35.0 20.0 35.0 100 31.431537788417685 35.0 33.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.5738848393110452 1101 2 1.1830350883275393 1101 3 1.5477494957046574 1101 4 0.9644653943321231 1101 5 0.5760270435096402 1101 6 1.1295631016845817 1101 7 0.8025094876185435 1101 8 0.4807261616980796 1101 9 0.5220607698114037 1101 10-11 0.426448804505668 1101 12-13 0.38450621446340705 1101 14-15 0.5152460211564431 1101 16-17 0.542017063367453 1101 18-19 0.5090344899740629 1101 20-21 0.5240626932077888 1101 22-23 0.5179490002121057 1101 24-25 0.5367661456976762 1101 26-27 0.5106001177166988 1101 28-29 0.4887055423207798 1101 30-31 0.5064671678438444 1101 32-33 0.5276272810794538 1101 34-35 0.5175048204564874 1101 36-37 0.5109753684041607 1101 38-39 0.5419315489149312 1101 40-41 0.5403838058047299 1101 42-43 0.5541277164626806 1101 44-45 0.5171921772259154 1101 46-47 0.5127794572754283 1101 48-49 0.48290533937422 1101 50-51 0.45571602466025496 1101 52-53 0.44306679280230554 1101 54-55 0.46196405792490225 1101 56-57 0.4681213433033875 1101 58-59 0.4637789001895243 1101 60-61 0.4584412696396001 1101 62-63 0.43523929500918257 1101 64-65 0.37930081661279047 1101 66-67 0.3889658620449623 1101 68-69 0.36759332854425253 1101 70-71 0.3561884376448319 1101 72-73 0.3301506628387614 1101 74-75 0.3371850161250691 1101 76-77 0.27191059710256127 1101 78-79 0.3919346228148015 1101 80-81 0.3751113856529287 1101 82-83 0.3438370910555548 1101 84-85 0.34547434537617505 1101 86-87 0.35540897512063907 1101 88-89 0.349138125316081 1101 90-91 0.3711551136431268 1101 92-93 0.35656031313446945 1101 94-95 0.3200319976396173 1101 96-97 0.34695333123783456 1101 98-99 0.38307495563803684 1101 100 0.3890860574857946 1102 1 1.4648118405241526 1102 2 1.1093661406877295 1102 3 1.4932362621946993 1102 4 0.9103157405737434 1102 5 0.49010144918747045 1102 6 1.0648385835694185 1102 7 0.7670392772010715 1102 8 0.43759590790382674 1102 9 0.4383130839806455 1102 10-11 0.3442930656743357 1102 12-13 0.33818365813846896 1102 14-15 0.4307020769841188 1102 16-17 0.4787758041920185 1102 18-19 0.4231964218599984 1102 20-21 0.41185640352874486 1102 22-23 0.42023938402063976 1102 24-25 0.4341227569867314 1102 26-27 0.41734084802815374 1102 28-29 0.4177050312461148 1102 30-31 0.4316062519485868 1102 32-33 0.4571431389324587 1102 34-35 0.42935568217833264 1102 36-37 0.45542822728827304 1102 38-39 0.45590087453001615 1102 40-41 0.4565873474509985 1102 42-43 0.47652189725428684 1102 44-45 0.46681334362281746 1102 46-47 0.4662571470477843 1102 48-49 0.44295590328667345 1102 50-51 0.4206944895518916 1102 52-53 0.4003287776490865 1102 54-55 0.42294543947569707 1102 56-57 0.43223321290238914 1102 58-59 0.4233387461336733 1102 60-61 0.41491428809653286 1102 62-63 0.37223020853851807 1102 64-65 0.36644672361445885 1102 66-67 0.3716172150863528 1102 68-69 0.3256922091719261 1102 70-71 0.3039501107758724 1102 72-73 0.29360668214590646 1102 74-75 0.3409496533129541 1102 76-77 0.17439334411689345 1102 78-79 0.3646541477052594 1102 80-81 0.3827003932666102 1102 82-83 0.34109151171773533 1102 84-85 0.35162039727499916 1102 86-87 0.37288658328390767 1102 88-89 0.36132489839014426 1102 90-91 0.3439524497351698 1102 92-93 0.35147865071896334 1102 94-95 0.31332838488162906 1102 96-97 0.35635919698710694 1102 98-99 0.36295774450209706 1102 100 0.3668144164238534 1103 1 1.4613590713411106 1103 2 1.0830847492035183 1103 3 1.436621922885017 1103 4 0.8946294781331403 1103 5 0.5081082951346261 1103 6 1.0390555661233094 1103 7 0.7511414071820113 1103 8 0.4224252605698382 1103 9 0.4224844725928776 1103 10-11 0.3452149381378007 1103 12-13 0.3172337351833576 1103 14-15 0.3619593889265147 1103 16-17 0.40717841636178065 1103 18-19 0.3537320580377141 1103 20-21 0.3944093907919992 1103 22-23 0.3821391669868106 1103 24-25 0.39423696636894334 1103 26-27 0.3955556269067486 1103 28-29 0.36870046288009917 1103 30-31 0.3558835360040149 1103 32-33 0.4104813639321705 1103 34-35 0.4036143767671234 1103 36-37 0.3547162715628076 1103 38-39 0.3780050107612425 1103 40-41 0.38896469628095787 1103 42-43 0.39677580463455087 1103 44-45 0.38888663534759615 1103 46-47 0.4135549713055866 1103 48-49 0.37994820873378643 1103 50-51 0.387200902958206 1103 52-53 0.38223880854186376 1103 54-55 0.3802522927186729 1103 56-57 0.38066414522326397 1103 58-59 0.38432195708297456 1103 60-61 0.355348047613937 1103 62-63 0.2867789235652012 1103 64-65 0.2841167842450574 1103 66-67 0.35261707774450457 1103 68-69 0.30118527122099437 1103 70-71 0.29116070369481406 1103 72-73 0.26938361194138594 1103 74-75 0.299548362389892 1103 76-77 0.17020524280741256 1103 78-79 0.3143114423805855 1103 80-81 0.32732298192202336 1103 82-83 0.34576186193336156 1103 84-85 0.3296419180196608 1103 86-87 0.30205662612907247 1103 88-89 0.29453546422016075 1103 90-91 0.29745217152019165 1103 92-93 0.30789434999582355 1103 94-95 0.30349497426909977 1103 96-97 0.32423540688536434 1103 98-99 0.352157498356533 1103 100 0.35643832747920356 1104 1 1.3855889361294977 1104 2 1.0282539106708306 1104 3 1.396380249452207 1104 4 0.8713001380967498 1104 5 0.4665450327183507 1104 6 1.000602122211717 1104 7 0.7316104059775768 1104 8 0.3956660895101365 1104 9 0.3830849029381369 1104 10-11 0.29664517535984203 1104 12-13 0.2876112072484247 1104 14-15 0.32826221084796714 1104 16-17 0.3602206213961594 1104 18-19 0.34052734284557573 1104 20-21 0.3271007855373469 1104 22-23 0.3314627889956725 1104 24-25 0.3186379005025941 1104 26-27 0.31967818617008703 1104 28-29 0.29259880963542173 1104 30-31 0.3082261106290147 1104 32-33 0.323692698936938 1104 34-35 0.3674759931276981 1104 36-37 0.3364956044735834 1104 38-39 0.40184679692597314 1104 40-41 0.366772233229824 1104 42-43 0.36678919127709975 1104 44-45 0.34401631249266984 1104 46-47 0.3723942689761728 1104 48-49 0.3159047713509082 1104 50-51 0.301526472293304 1104 52-53 0.3577665138248918 1104 54-55 0.32979198075977934 1104 56-57 0.3878551938086616 1104 58-59 0.3832662097110955 1104 60-61 0.3526582831606362 1104 62-63 0.3120653939987861 1104 64-65 0.27731501197334296 1104 66-67 0.26583424929257404 1104 68-69 0.24177496735850212 1104 70-71 0.23778537753612738 1104 72-73 0.25713822603980674 1104 74-75 0.2818978883165997 1104 76-77 0.17404183271591478 1104 78-79 0.26379880090961194 1104 80-81 0.29897136686884096 1104 82-83 0.3077692049267071 1104 84-85 0.26032774878993337 1104 86-87 0.27001109201489015 1104 88-89 0.2640230668444019 1104 90-91 0.2726893826201362 1104 92-93 0.25946405473115774 1104 94-95 0.25137736741363526 1104 96-97 0.24716954054716567 1104 98-99 0.2607900687015139 1104 100 0.2756813082075169 1105 1 1.315168727983167 1105 2 0.9718864476249927 1105 3 1.3326147022062287 1105 4 0.8314088462401017 1105 5 0.41566032441527767 1105 6 0.983515633698623 1105 7 0.6912844019989564 1105 8 0.3401740670441953 1105 9 0.34312481655627636 1105 10-11 0.24729645196654815 1105 12-13 0.2233438264899874 1105 14-15 0.2705041483230275 1105 16-17 0.250405869128393 1105 18-19 0.25206492275702175 1105 20-21 0.26827815896115936 1105 22-23 0.29040141512593465 1105 24-25 0.28232616683093426 1105 26-27 0.28563414787389974 1105 28-29 0.28477443021449744 1105 30-31 0.2986784745897779 1105 32-33 0.3146484497170903 1105 34-35 0.31252515268619163 1105 36-37 0.27530335583065835 1105 38-39 0.3438179890381221 1105 40-41 0.3485608613962725 1105 42-43 0.32082560211015476 1105 44-45 0.28306798159290736 1105 46-47 0.309467195654193 1105 48-49 0.2756897190129237 1105 50-51 0.26208626219533926 1105 52-53 0.22596081977319216 1105 54-55 0.2849850012185158 1105 56-57 0.31374307799409706 1105 58-59 0.26831335375695176 1105 60-61 0.27259400253556265 1105 62-63 0.23039863026713903 1105 64-65 0.24620331113717242 1105 66-67 0.24245059166555194 1105 68-69 0.2008167104398879 1105 70-71 0.2164570924369542 1105 72-73 0.20076236302011097 1105 74-75 0.19925405309692223 1105 76-77 0.08906624458032297 1105 78-79 0.20403803262823317 1105 80-81 0.25848915197485667 1105 82-83 0.2651148263745142 1105 84-85 0.25817370407624196 1105 86-87 0.26508911270219215 1105 88-89 0.27658301048239053 1105 90-91 0.256936032366049 1105 92-93 0.25089047087555727 1105 94-95 0.22580474128068317 1105 96-97 0.24312441569340493 1105 98-99 0.23708311208611832 1105 100 0.23623004722386298 1106 1 1.3104225313336961 1106 2 0.9604856192688693 1106 3 1.3223441309439607 1106 4 0.793839925134769 1106 5 0.4081986595216023 1106 6 0.943689176412633 1106 7 0.6682376524470826 1106 8 0.33031049232214116 1106 9 0.28616297391039325 1106 10-11 0.21800029154215395 1106 12-13 0.20617341213287688 1106 14-15 0.2305331341014636 1106 16-17 0.23403233061689122 1106 18-19 0.20786395554741688 1106 20-21 0.2188391766476343 1106 22-23 0.2510036697856748 1106 24-25 0.24793709221633975 1106 26-27 0.2074792532484011 1106 28-29 0.2343562393272549 1106 30-31 0.25191401586608464 1106 32-33 0.273826938707451 1106 34-35 0.2692779397409524 1106 36-37 0.25040642228401566 1106 38-39 0.2905980896135816 1106 40-41 0.28976541894230934 1106 42-43 0.2890519121699313 1106 44-45 0.28940442812685774 1106 46-47 0.2746428090500004 1106 48-49 0.24733366211247443 1106 50-51 0.2723378640066443 1106 52-53 0.23609807771603641 1106 54-55 0.2893456577761526 1106 56-57 0.31456388215164566 1106 58-59 0.26944115664340984 1106 60-61 0.24185615459015963 1106 62-63 0.2310570329883106 1106 64-65 0.16200214910436017 1106 66-67 0.16086205742253412 1106 68-69 0.17963010212095298 1106 70-71 0.18418948043594696 1106 72-73 0.12407286142353513 1106 74-75 0.1757864317788389 1106 76-77 0.012959487342058651 1106 78-79 0.15945511036974125 1106 80-81 0.226430417489901 1106 82-83 0.22798490319411968 1106 84-85 0.21633943188764704 1106 86-87 0.21442951575141933 1106 88-89 0.2121220415982421 1106 90-91 0.21537978765642407 1106 92-93 0.21695241383269703 1106 94-95 0.19674558212620497 1106 96-97 0.225599310774399 1106 98-99 0.23315486433955357 1106 100 0.24498965068286793 1107 1 1.2616823621646525 1107 2 0.8931127189646055 1107 3 1.2788568376791005 1107 4 0.7834301421454057 1107 5 0.38049006700153143 1107 6 0.913240017100712 1107 7 0.6445705162826769 1107 8 0.2933361205286502 1107 9 0.2719351484698507 1107 10-11 0.18320975017552144 1107 12-13 0.10803038920536778 1107 14-15 0.1684794203434663 1107 16-17 0.17664720619676189 1107 18-19 0.15171228634677902 1107 20-21 0.15522576929954823 1107 22-23 0.19659257045535128 1107 24-25 0.18618468781179587 1107 26-27 0.16892921931945182 1107 28-29 0.14256970378855272 1107 30-31 0.1455583715887414 1107 32-33 0.18077295802428495 1107 34-35 0.19348153151270964 1107 36-37 0.18380516826918836 1107 38-39 0.2207928812583404 1107 40-41 0.21872220226632066 1107 42-43 0.2552723258894716 1107 44-45 0.21258893185498096 1107 46-47 0.2649849120481349 1107 48-49 0.23659129004275314 1107 50-51 0.2088114827298 1107 52-53 0.20197990139927668 1107 54-55 0.2564996545820293 1107 56-57 0.2878173169970566 1107 58-59 0.23040179784950965 1107 60-61 0.2517428937700146 1107 62-63 0.21962967716125803 1107 64-65 0.1905631223553712 1107 66-67 0.21057877031520178 1107 68-69 0.21474702049933825 1107 70-71 0.18857761094586323 1107 72-73 0.17932664290648148 1107 74-75 0.17557204884829503 1107 76-77 0.04113002822106182 1107 78-79 0.19169686227870386 1107 80-81 0.2464704228178789 1107 82-83 0.25329132202519844 1107 84-85 0.24923989545069958 1107 86-87 0.2161808247963961 1107 88-89 0.23760751074264164 1107 90-91 0.2586503324261997 1107 92-93 0.2333744193403966 1107 94-95 0.17458091738831882 1107 96-97 0.21666906709953793 1107 98-99 0.24574700587520226 1107 100 0.25249187992221067 1108 1 1.239396114205487 1108 2 0.8620661232499707 1108 3 1.22317058726329 1108 4 0.7379203047796494 1108 5 0.3340024694766086 1108 6 0.8599030858932011 1108 7 0.6044521270478214 1108 8 0.25837062874766303 1108 9 0.2288276761891055 1108 10-11 0.13889670475187188 1108 12-13 0.10779761483949102 1108 14-15 0.1213133555603747 1108 16-17 0.13612341978710418 1108 18-19 0.09834438967778425 1108 20-21 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-0.764378679562256 2316 96-97 -0.8845340035719005 2316 98-99 -1.030308148362355 2316 100 -0.9515579334594939 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 78.0 7 1052.0 8 1383.0 9 522.0 10 500.0 11 864.0 12 1127.0 13 1618.0 14 3425.0 15 7584.0 16 14145.0 17 22654.0 18 31928.0 19 46317.0 20 66376.0 21 98667.0 22 154904.0 23 263205.0 24 483262.0 25 713503.0 26 733512.0 27 574336.0 28 460589.0 29 456962.0 30 519221.0 31 631757.0 32 781081.0 33 998647.0 34 1368372.0 35 2158010.0 36 3841782.0 37 7626828.0 38 1.0327558E7 39 3306475.0 40 8001.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.21410121963967 35.78636467349827 27.035525897251738 20.964008209610324 2 20.074809712771035 33.332790909233495 28.24784785536154 18.344551522633928 3 25.698171392692753 26.406463187383977 26.751824148101544 21.143541271821725 4 18.583595775682905 27.06025051240078 26.72288364817313 27.633270063743183 5 20.110866370122704 34.10159331023167 26.328971900113 19.458568419532625 6 28.426475998885024 25.99992975381794 26.4041001811388 19.169494066158236 7 19.77326485042492 26.44885557040657 34.2581948602416 19.51968471892691 8 27.157588181887586 26.003722272767526 26.901724638859054 19.936964906485837 9 27.12242354243634 25.566849720546088 27.4297115252528 19.881015211764776 10-11 19.323463763698857 30.44520327587205 30.56554926323406 19.66578369719503 12-13 19.425422639653092 26.520393001280308 30.219741672640176 23.83444268642642 14-15 19.433923953638924 34.646930810002566 26.13262890007056 19.786516336287953 16-17 23.69064107098612 26.361110397728037 26.100429020333788 23.84781951095205 18-19 19.62125953037067 30.50743924487159 26.080699608709907 23.790601616047837 20-21 23.719415848869563 30.47150872791852 26.066662238856335 19.74241318435558 22-23 23.611579419540206 26.465377041991427 30.197942916317803 19.725100622150556 24-25 19.610903335940453 30.39504523363441 26.028088002869428 23.965963427555707 26-27 19.66454269365284 26.35002548171062 34.217347669636546 19.768084154999997 28-29 19.670695321611916 26.423221140310048 34.195796033317535 19.710287504760494 30-31 19.68979110626973 26.400488782407887 34.12978282601857 19.779937285303806 32-33 19.780492021841837 26.44869880543834 34.021946736276774 19.74886243644304 34-35 19.7664103606292 26.519498324167195 33.99200113638475 19.722090178818846 36-37 19.749207714249046 26.517405149596556 33.94252914850454 19.790857987649858 38-39 19.790277991123183 26.508339968960932 33.914738058807636 19.786643981108245 40-41 19.783548023612248 26.566344253014805 33.904762099488714 19.74534562388423 42-43 19.784279086045476 26.600337938329687 33.835060537269904 19.78032243835494 44-45 19.79462838816597 26.634794624606016 33.783948547736856 19.786628439491153 46-47 19.81923687982059 26.715754936878856 33.72382850014156 19.741179683158997 48-49 19.844899412779096 26.75257907357193 33.613624949356556 19.78889656429242 50-51 19.85859381812871 26.805796419281847 33.53695958007235 19.798650182517093 52-53 19.95839578566067 26.962886748458022 33.25344900085851 19.8252684650228 54-55 20.336657778432222 27.23704976733311 32.36765184702196 20.05864060721271 56-57 20.876706443829843 27.84707272665085 30.9619391002185 20.314281729300806 58-59 20.96397833615045 28.534175890445017 30.138523551023273 20.363322222381264 60-61 20.900124986624583 28.93282375093208 29.74869060964453 20.41836065279881 62-63 20.82795869459312 29.152020917624988 29.57130121707458 20.448719170707314 64-65 20.803949642171585 29.204452519423352 29.586847186892555 20.404750651512508 66-67 20.742455248398713 28.96427465248927 29.876244616215462 20.417025482896552 68-69 20.658183735294656 28.728080074792366 30.240909198533352 20.37282699137963 70-71 20.607154013481917 28.598989042519406 30.470524519839753 20.323332424158924 72-73 20.559244244673884 28.56092555936602 30.49944984251127 20.380380353448828 74-75 20.662960412450015 28.629774168383687 30.292663514372155 20.414601904794143 76-77 20.727467701532106 28.823120885006336 30.02820492938511 20.421206484076443 78-79 20.841471012563332 29.035328227403724 29.61373977446161 20.509460985571334 80-81 20.932009371733297 29.25466256733194 29.24584721555093 20.56748084538383 82-83 21.00033035379799 29.467537765827633 28.865863749092508 20.66626813128187 84-85 21.029906701038865 29.55939852567251 28.6164364693964 20.794258303892228 86-87 21.105440218861197 29.587003789513812 28.44554132477836 20.86201466684663 88-89 21.143830730575043 29.67092183452296 28.277817513428367 20.90742992147362 90-91 21.235449532577118 29.678295364216385 28.052486693155537 21.033768410050964 92-93 21.268999598968584 29.72625472248822 27.91516630192705 21.08957937661614 94-95 21.327054611433173 29.75298987190902 27.796997763977465 21.122957752680342 96-97 21.343304869814407 29.747065971321085 27.663861327143845 21.24576783172066 98-99 21.35765999483738 29.7383882185771 27.61309204210373 21.29085974448179 100 21.35959782342008 29.762243262046645 27.530624259881044 21.347534654652232 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 92198.0 1 60987.0 2 23091.0 3 15358.5 4 14044.5 5 13706.5 6 13878.0 7 14003.0 8 14209.5 9 14881.5 10 16872.0 11 32740.0 12 239394.0 13 275140.0 14 114126.5 15 117785.0 16 134528.0 17 150547.5 18 165148.5 19 179127.5 20 196718.5 21 223127.5 22 257646.0 23 292896.5 24 325945.5 25 367548.5 26 420084.0 27 479873.0 28 548564.5 29 613114.5 30 665056.5 31 717792.0 32 776891.0 33 831102.5 34 871129.0 35 920392.5 36 988030.0 37 1040716.0 38 1075807.5 39 1094101.5 40 1097748.5 41 1103454.0 42 1121451.5 43 1130377.5 44 1131690.5 45 1141155.0 46 1137847.0 47 1123952.5 48 1097330.0 49 1063479.5 50 1039793.0 51 1019811.0 52 990810.0 53 943927.5 54 882021.5 55 822038.0 56 761807.5 57 695406.0 58 621885.5 59 537686.5 60 445883.5 61 360865.5 62 285040.0 63 214072.5 64 156316.0 65 112045.5 66 78989.5 67 56937.5 68 41737.5 69 30581.0 70 22902.5 71 17621.0 72 14145.0 73 11143.5 74 8845.0 75 7340.0 76 5653.5 77 3581.0 78 2147.0 79 1491.0 80 1000.0 81 668.5 82 449.0 83 305.5 84 208.5 85 139.5 86 86.5 87 51.5 88 35.5 89 25.5 90 18.0 91 14.0 92 9.0 93 5.0 94 6.0 95 4.0 96 1.5 97 2.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33239283492285454 2 0.006511465991453316 3 2.4315130308437642 4 8.625942044591919E-4 5 4.481008854333465E-5 6 0.00906564103842339 7 0.007668126401978142 8 2.884649449977168E-4 9 0.0 10-11 7.701733968385643E-5 12-13 0.0 14-15 0.0 16-17 3.3607566407500985E-5 18-19 0.0 20-21 3.038684129344881E-4 22-23 4.6210403810313854E-5 24-25 5.097147571804316E-4 26-27 0.0034293720888320797 28-29 0.00623840451439237 30-31 0.005640469895392249 32-33 0.015382463207766598 34-35 0.009938037449751436 36-37 0.005034133384790251 38-39 0.005280588871778592 40-41 0.008375285611802641 42-43 0.019133907808003895 44-45 0.03435253412953392 46-47 0.033345707452575875 48-49 0.0400784232562119 50-51 0.04028006865465691 52-53 0.06262209873931017 54-55 0.033235082546484516 56-57 0.022281816528173154 58-59 0.016899004641905076 60-61 0.01799405118068282 62-63 0.02488220197895354 64-65 0.04487870399141663 66-67 0.04797760167724161 68-69 0.050719418969986906 70-71 0.06121058095019513 72-73 0.0690187388788712 74-75 0.05976265496413863 76-77 0.05978786063894425 78-79 0.054251014073308464 80-81 0.05182006676983256 82-83 0.04826886725277329 84-85 0.05014669002579241 86-87 0.05824331289946619 88-89 0.06498303027943712 90-91 0.060761079749494795 92-93 0.06512866306720295 94-95 0.07385822844155132 96-97 0.07268476424782275 98-99 0.04763172380629775 100 0.007071592098244999 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.5706245E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.86552622313562 #Duplication Level Percentage of deduplicated Percentage of total 1 69.48812927739881 25.617164520725836 2 15.067342999747757 11.109310569403595 3 5.431795029002831 6.007379462412047 4 2.6582313508774233 3.9198839029173147 5 1.614055959862061 2.975151115695157 6 1.0318772100809657 2.2824417808377513 7 0.7933710269209358 2.047362827834086 8 0.5567889502356694 1.6421054116532177 9 0.4400452622805754 1.460025014037403 >10 2.511317976592384 16.92683601820253 >50 0.22816787247651077 6.02207651243334 >100 0.1761646028351165 11.387081248223497 >500 0.0020564306218149044 0.44574487426993703 >1k 5.495941759122147E-4 0.5321307571696875 >5k 4.56243823400809E-5 0.11742371286047946 >10k+ 6.0832508820745584E-5 7.507882271324133 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2400154 6.721944578602426 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 92791 0.25987330787653534 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 60519 0.16949135928462936 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 52305 0.14648698007869493 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.545248457237662E-4 1.1202522135833661E-5 0.0 2.8006305339584153E-6 0.0 2 5.657273678595999E-4 2.2405044271667322E-5 0.0 2.8006305339584153E-6 0.0 3 5.713286289275167E-4 2.2405044271667322E-5 0.0 1.1202522135833661E-5 0.0 4 5.713286289275167E-4 3.3607566407500985E-5 0.0 1.4003152669792078E-5 5.6012610679168305E-6 5 5.713286289275167E-4 3.920882747541782E-5 0.0 1.4003152669792078E-5 5.6012610679168305E-6 6 5.713286289275167E-4 4.7610719077293064E-5 0.0 1.4003152669792078E-5 8.401891601875246E-6 7 5.713286289275167E-4 5.321198014520989E-5 0.0 1.4003152669792078E-5 8.401891601875246E-6 8 5.713286289275167E-4 5.321198014520989E-5 0.0 1.4003152669792078E-5 1.1202522135833661E-5 9 5.741292594614751E-4 5.321198014520989E-5 0.0 1.6803783203750492E-5 1.4003152669792078E-5 10-11 5.783302052624127E-4 6.301418701406435E-5 0.0 1.6803783203750492E-5 1.4003152669792078E-5 12-13 5.825311510633504E-4 6.721513281500197E-5 0.0 1.6803783203750492E-5 1.4003152669792078E-5 14-15 5.839314663303296E-4 7.281639388291881E-5 0.0 2.1004729004688116E-5 1.4003152669792078E-5 16-17 5.923333579322048E-4 7.841765495083564E-5 0.0 2.660599007260495E-5 1.4003152669792078E-5 18-19 6.007352495340801E-4 8.401891601875246E-5 0.0 2.8006305339584155E-5 1.4003152669792078E-5 20-21 6.049361953350177E-4 9.242080762062771E-5 0.0 2.8006305339584155E-5 2.1004729004688116E-5 22-23 6.175390327378305E-4 9.382112288760692E-5 0.0 2.9406620606563362E-5 2.2405044271667322E-5 24-25 6.791529044849157E-4 1.0222301448948216E-4 0.0 3.2207251140521775E-5 2.660599007260495E-5 26-27 9.536146968128405E-4 1.2042711296021185E-4 0.0 3.5007881674480195E-5 3.5007881674480195E-5 28-29 0.0018316123692088035 1.3162963509604552E-4 0.0 3.780851220843861E-5 4.060914274239702E-5 30-31 0.0038746723437314678 1.4003152669792078E-4 0.0 4.060914274239702E-5 4.901103434427227E-5 32-33 0.008698758438474837 1.456327877658376E-4 0.0 4.200945800937623E-5 5.041134961125148E-5 34-35 0.017705586235685102 1.5403467936771285E-4 0.0 4.200945800937623E-5 5.321198014520989E-5 36-37 0.032196048618385945 1.5403467936771285E-4 0.0 4.200945800937623E-5 5.8813241213126724E-5 38-39 0.05402136236952387 1.5683530990167127E-4 0.0 4.200945800937623E-5 6.301418701406435E-5 40-41 0.08519238021248104 1.5823562516865047E-4 0.0 4.200945800937623E-5 7.981797021781485E-5 42-43 0.12695258210433497 1.6243657096958808E-4 0.0 4.340977327635544E-5 8.401891601875246E-5 44-45 0.17998952284117245 1.6243657096958808E-4 0.0 4.7610719077293064E-5 8.962017708666929E-5 46-47 0.2468629787310315 1.6803783203750492E-4 0.0 4.7610719077293064E-5 9.242080762062771E-5 48-49 0.32851956289439005 1.7784003890635938E-4 0.0 5.041134961125148E-5 9.802206868854453E-5 50-51 0.4277599058652065 2.0024508317802669E-4 0.0 5.321198014520989E-5 1.0642396029041978E-4 52-53 0.5440868957237033 2.1004729004688114E-4 0.0 5.321198014520989E-5 1.092245908243782E-4 54-55 0.6783561250980046 2.184491816487564E-4 0.0 5.321198014520989E-5 1.1482585189229503E-4 56-57 0.8327310810755935 2.2825138851761086E-4 0.0 5.321198014520989E-5 1.2042711296021187E-4 58-59 1.00893135080432 2.352529648525069E-4 0.0 5.321198014520989E-5 1.2042711296021187E-4 60-61 1.2075744733169227 2.4225454118740293E-4 2.8006305339584153E-6 5.321198014520989E-5 1.2182742822719108E-4 62-63 1.4264059410335643 2.6325927019209104E-4 2.8006305339584153E-6 5.8813241213126724E-5 1.232277434941703E-4 64-65 1.6686982907331758 2.89865260264696E-4 2.8006305339584153E-6 6.161387174708514E-5 1.2602837402812868E-4 66-67 1.9321340006489063 3.066690434684465E-4 2.8006305339584153E-6 6.441450228104355E-5 1.2742868929510788E-4 68-69 2.2210792537831967 3.108699892693841E-4 2.8006305339584153E-6 6.861544808198118E-5 1.3443026563000394E-4 70-71 2.5357342952192257 3.220725114052178E-4 2.8006305339584153E-6 7.141607861593959E-5 1.3583058089698316E-4 72-73 2.8735477505405567 3.3187471827407223E-4 2.8006305339584153E-6 7.4216709149898E-5 1.4283215723187917E-4 74-75 3.23519177107534 3.346753488080306E-4 2.8006305339584153E-6 7.841765495083562E-5 1.442324724988584E-4 76-77 3.6222025026714517 3.416769251429267E-4 5.6012610679168305E-6 8.261860075177325E-5 1.456327877658376E-4 78-79 4.036377950131692 3.4727818621084353E-4 5.6012610679168305E-6 8.541923128573167E-5 1.456327877658376E-4 80-81 4.478387464153679 3.5848070834667715E-4 5.6012610679168305E-6 8.681954655271088E-5 1.48433418299796E-4 82-83 4.944989314894356 3.7248386101646923E-4 5.6012610679168305E-6 8.962017708666929E-5 1.5263436410073363E-4 84-85 5.4341278395417945 3.794854373513653E-4 5.6012610679168305E-6 8.962017708666929E-5 1.596359404356297E-4 86-87 5.9491904007268195 3.8928764422021975E-4 5.6012610679168305E-6 9.382112288760692E-5 1.6243657096958808E-4 88 6.350684593129297 4.004901663560534E-4 5.6012610679168305E-6 9.802206868854453E-5 1.6243657096958808E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 312340 0.0 86.83171 8 AAGCAGT 302020 0.0 86.67169 1 GTGGTAT 313310 0.0 85.91452 6 GCAGTGG 313880 0.0 85.7136 3 TGGTATC 316945 0.0 85.57681 7 AGTGGTA 314920 0.0 85.567955 5 GTATCAA 318420 0.0 85.07902 9 AGCAGTG 319335 0.0 83.939964 2 CAGTGGT 326705 0.0 82.241806 4 CAACGCA 308175 0.0 45.231537 12-13 ATCAACG 310160 0.0 45.009438 10-11 ACGCAGA 311685 0.0 44.845753 14-15 AGAGTAC 316235 0.0 44.145283 18-19 TCAACGC 307695 0.0 43.984062 12-13 AACGCAG 309900 0.0 43.777985 14-15 CGCAGAG 313180 0.0 43.33088 16-17 GAGTACT 311315 0.0 43.28279 20-21 TATCAAC 315775 0.0 42.87573 10-11 AGTACTT 326050 0.0 42.585735 20-21 GTACTTT 323290 0.0 41.66513 22-23 >>END_MODULE