FastQCFastQC Report
Thu 26 May 2016
SRR1660707_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660707_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35580109
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23328436.556593179633036No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA498950.14023284751600956No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT388030.10905812570725965No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2936400.086.5438848
AGTGGTA2951450.086.266615
TGGTATC2953100.085.8281257
GTATCAA2968950.085.475039
GTGGTAT2990350.085.133356
AAGCAGT3024150.084.693121
GCAGTGG3046050.084.125873
CAGTGGT3080800.082.558434
AGCAGTG3140050.081.686332
CAACGCA2899000.044.8576912-13
ATCAACG2920350.044.63264510-11
ACGCAGA2926700.044.45985814-15
AGAGTAC2922850.043.90664318-19
TCAACGC2900250.043.89748412-13
GAGTACT2846350.043.86605520-21
AACGCAG2918450.043.73176614-15
AGTACTT2944400.043.25461620-21
CGCAGAG2947400.043.19147516-17
TATCAAC2961500.042.75710310-11
GTACTTT2950800.042.2482122-23
Produced by FastQC (version 0.11.5)