##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660707_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 35580109 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.810396336897114 34.0 31.0 34.0 30.0 34.0 2 31.929737061794835 34.0 31.0 34.0 30.0 34.0 3 31.99361421293004 34.0 31.0 34.0 30.0 34.0 4 35.40024287165618 37.0 35.0 37.0 33.0 37.0 5 35.36555329271195 37.0 35.0 37.0 33.0 37.0 6 35.454868702060466 37.0 35.0 37.0 33.0 37.0 7 35.393387693106845 37.0 35.0 37.0 33.0 37.0 8 35.413509104202014 37.0 35.0 37.0 33.0 37.0 9 37.088518306675226 39.0 37.0 39.0 33.0 39.0 10-11 37.001625402552875 39.0 37.0 39.0 33.0 39.0 12-13 37.04898624678188 39.0 37.0 39.0 33.0 39.0 14-15 38.422373396888695 40.0 38.0 41.0 33.0 41.0 16-17 38.39834202025632 40.0 38.0 41.0 33.0 41.0 18-19 38.35505377456826 40.0 38.0 41.0 33.5 41.0 20-21 38.22132007802449 40.0 38.0 41.0 33.0 41.0 22-23 38.02479520228564 40.0 38.0 41.0 32.5 41.0 24-25 38.019892870479964 40.0 38.0 41.0 33.0 41.0 26-27 38.024152005830004 40.0 38.0 41.0 33.0 41.0 28-29 37.949653948502515 40.0 38.0 41.0 33.0 41.0 30-31 37.85751597894206 40.0 38.0 41.0 32.0 41.0 32-33 37.72921298526657 40.0 38.0 41.0 32.0 41.0 34-35 37.5701395406068 40.0 37.5 41.0 31.5 41.0 36-37 37.367190401243576 40.0 37.0 41.0 31.0 41.0 38-39 36.88582832896886 39.0 35.0 41.0 30.5 41.0 40-41 36.818564903778125 39.0 35.0 41.0 30.0 41.0 42-43 36.81581394255987 39.0 35.0 41.0 30.0 41.0 44-45 36.81065250530851 39.5 35.0 41.0 30.0 41.0 46-47 36.89477067088243 40.0 35.0 41.0 30.0 41.0 48-49 36.782110799604354 40.0 35.0 41.0 30.0 41.0 50-51 35.41102069136438 38.5 34.0 40.0 28.0 40.5 52-53 35.54993665140261 38.5 34.5 39.5 28.0 40.5 54-55 35.85240649487611 39.0 35.0 41.0 26.0 41.0 56-57 35.40295265818325 39.0 35.0 41.0 23.0 41.0 58-59 35.19259849653636 39.0 35.0 41.0 22.0 41.0 60-61 34.9684110017763 39.0 35.0 41.0 22.0 41.0 62-63 34.74018002586783 38.0 34.0 41.0 22.0 41.0 64-65 34.48709557073026 37.5 34.0 40.0 21.5 41.0 66-67 34.22704265745785 37.0 34.0 40.0 21.0 41.0 68-69 33.96061563498864 36.5 34.0 40.0 21.0 41.0 70-71 33.66674480957886 36.0 33.0 39.0 21.0 41.0 72-73 33.32801564773172 35.5 33.0 39.0 20.0 41.0 74-75 32.94686477773297 35.0 33.0 38.5 20.0 40.0 76-77 32.545790036787125 35.0 33.0 37.0 19.5 39.5 78-79 32.16877018280073 35.0 32.0 37.0 18.5 39.0 80-81 31.845478312053515 35.0 32.0 36.0 18.0 39.0 82-83 31.533042689666857 35.0 32.0 36.0 17.5 37.5 84-85 31.24981307673903 35.0 31.5 35.5 17.0 37.0 86-87 31.035525790547748 35.0 31.0 35.0 15.5 37.0 88-89 30.83219237748822 35.0 31.0 35.0 15.0 36.0 90-91 30.66022858445993 35.0 31.0 35.0 15.0 36.0 92-93 30.505497341225123 35.0 31.0 35.0 13.5 36.0 94-95 30.322825556830082 35.0 30.5 35.0 11.0 35.5 96-97 30.184184680266156 34.5 30.0 35.0 10.0 35.0 98-99 30.075935180524603 34.0 30.0 35.0 10.0 35.0 100 29.991172090001186 34.0 30.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5110340946502383 1101 2 0.5077175336666429 1101 3 0.5141291242538912 1101 4 0.46628473190278186 1101 5 0.47022904096786533 1101 6 0.452273001621883 1101 7 0.4731991979049539 1101 8 0.493285187554072 1101 9 0.5536119105916271 1101 10-11 0.5824491386878847 1101 12-13 0.5573680458556467 1101 14-15 0.6708547166975265 1101 16-17 0.6292850374127852 1101 18-19 0.6409784232813607 1101 20-21 0.6683869234595079 1101 22-23 0.7198334025247988 1101 24-25 0.7231145655497428 1101 26-27 0.7108214448148615 1101 28-29 0.6785784083012558 1101 30-31 0.6691702648003499 1101 32-33 0.6365734307942219 1101 34-35 0.6717082739018352 1101 36-37 0.6097747254086983 1101 38-39 0.6364761454881531 1101 40-41 0.6280162245621099 1101 42-43 0.6453348464858593 1101 44-45 0.6725994541598439 1101 46-47 0.6886766961201829 1101 48-49 0.7241415380327894 1101 50-51 0.6754508465304667 1101 52-53 0.7195698393925056 1101 54-55 0.715354169597127 1101 56-57 0.7247708136294833 1101 58-59 0.6737716948904122 1101 60-61 0.6561239033763684 1101 62-63 0.627724069651002 1101 64-65 0.6162959791169058 1101 66-67 0.5712629504841544 1101 68-69 0.5894296202795175 1101 70-71 0.5531610603620081 1101 72-73 0.5555353479084673 1101 74-75 0.5299961231774404 1101 76-77 0.5339919340408272 1101 78-79 0.5134459109507574 1101 80-81 0.47157441653870436 1101 82-83 0.5257890084742733 1101 84-85 0.5242678494951072 1101 86-87 0.4753264475011143 1101 88-89 0.439460548887336 1101 90-91 0.4355429254929142 1101 92-93 0.4324711382565063 1101 94-95 0.4634463719000834 1101 96-97 0.44333872223383963 1101 98-99 0.4193858562054942 1101 100 0.37124030023277044 1102 1 0.44992373547797015 1102 2 0.4732397397703778 1102 3 0.4857362026591332 1102 4 0.4307923025773235 1102 5 0.41909184914711517 1102 6 0.3867003192130909 1102 7 0.4142435291344029 1102 8 0.4411714874596768 1102 9 0.48221681145908946 1102 10-11 0.48313209196433604 1102 12-13 0.5061306678239887 1102 14-15 0.598061637928275 1102 16-17 0.5627190727099531 1102 18-19 0.5418617248790127 1102 20-21 0.5620204259598012 1102 22-23 0.5904740370686383 1102 24-25 0.5644165466965134 1102 26-27 0.5603605063026365 1102 28-29 0.5849795190884564 1102 30-31 0.5514933538345304 1102 32-33 0.5511830868072707 1102 34-35 0.5420832715881758 1102 36-37 0.550628565463974 1102 38-39 0.5571361135365365 1102 40-41 0.5478597846493543 1102 42-43 0.5708795972586174 1102 44-45 0.572373139847862 1102 46-47 0.570202633575839 1102 48-49 0.6329218845493472 1102 50-51 0.5656979972606564 1102 52-53 0.5914369295742716 1102 54-55 0.6544963124531691 1102 56-57 0.6664001629208869 1102 58-59 0.5304526811719583 1102 60-61 0.5428729237350112 1102 62-63 0.5423775405621569 1102 64-65 0.5077171425706695 1102 66-67 0.5047226288239983 1102 68-69 0.47294976221472496 1102 70-71 0.4801611743096643 1102 72-73 0.45526365215911113 1102 74-75 0.4571162581331052 1102 76-77 0.43444971537392263 1102 78-79 0.4451220722913902 1102 80-81 0.41675518263713585 1102 82-83 0.4179114513037838 1102 84-85 0.36158862776338196 1102 86-87 0.3402652647437314 1102 88-89 0.3470602791148174 1102 90-91 0.3199645987226525 1102 92-93 0.304129926917728 1102 94-95 0.38026263101589564 1102 96-97 0.3361033497580408 1102 98-99 0.27427983090024455 1102 100 0.2594239090456476 1103 1 0.3842042803907013 1103 2 0.40922567096703943 1103 3 0.4239773995210214 1103 4 0.35064928325805766 1103 5 0.39754012500917923 1103 6 0.34814667936634436 1103 7 0.33499374705895235 1103 8 0.36499925611502704 1103 9 0.4324907375471909 1103 10-11 0.4271495563362109 1103 12-13 0.4194594146057966 1103 14-15 0.5113979867291789 1103 16-17 0.4721980303210884 1103 18-19 0.47699242892733906 1103 20-21 0.5189942648780743 1103 22-23 0.5413897002687804 1103 24-25 0.5774527424434339 1103 26-27 0.5201686313406455 1103 28-29 0.5042308722478523 1103 30-31 0.49628749115740334 1103 32-33 0.4727859978737783 1103 34-35 0.460344461760279 1103 36-37 0.4607562287046676 1103 38-39 0.49401844948496887 1103 40-41 0.49277302027140735 1103 42-43 0.49043313067709704 1103 44-45 0.5041152438840228 1103 46-47 0.537098374417937 1103 48-49 0.5692720791610313 1103 50-51 0.5132839760966306 1103 52-53 0.5145558099488952 1103 54-55 0.6420378701575586 1103 56-57 0.6895698501231422 1103 58-59 0.6120464191354316 1103 60-61 0.6080228608916585 1103 62-63 0.5545262005795948 1103 64-65 0.544576165660537 1103 66-67 0.53440154487744 1103 68-69 0.5080666559283671 1103 70-71 0.4928622477147542 1103 72-73 0.4639755660434375 1103 74-75 0.4879519480923662 1103 76-77 0.500031700615871 1103 78-79 0.4965991446017952 1103 80-81 0.4528590718301082 1103 82-83 0.46607974480253844 1103 84-85 0.42441171357497254 1103 86-87 0.39556108464682893 1103 88-89 0.3831869743650742 1103 90-91 0.39620461473757373 1103 92-93 0.3583798356935013 1103 94-95 0.4127346555856235 1103 96-97 0.39544851183308793 1103 98-99 0.37832993327410236 1103 100 0.3927949990738284 1104 1 0.38213455079268854 1104 2 0.399676418009534 1104 3 0.3862363240818496 1104 4 0.3388332327666177 1104 5 0.3664463399457887 1104 6 0.2936140361587718 1104 7 0.3237430164352091 1104 8 0.33840335403744604 1104 9 0.4016543805208812 1104 10-11 0.35284704063223415 1104 12-13 0.37793289813790665 1104 14-15 0.45159964350785486 1104 16-17 0.4462245887834797 1104 18-19 0.4411814988855909 1104 20-21 0.377230986961024 1104 22-23 0.4315048635870866 1104 24-25 0.4183209440354645 1104 26-27 0.3943689323564641 1104 28-29 0.3991806930513846 1104 30-31 0.3590753313434263 1104 32-33 0.36631272830127415 1104 34-35 0.3515483563649866 1104 36-37 0.34912758294561996 1104 38-39 0.35865040390505953 1104 40-41 0.35420465508546073 1104 42-43 0.3450846577497728 1104 44-45 0.37129080079180454 1104 46-47 0.4286389619467883 1104 48-49 0.4706496227199395 1104 50-51 0.42039781781535623 1104 52-53 0.40454610390159473 1104 54-55 0.5153014760473624 1104 56-57 0.5665748413755054 1104 58-59 0.5323280351071631 1104 60-61 0.5517410380495491 1104 62-63 0.49406998421797965 1104 64-65 0.45338116521703853 1104 66-67 0.44559517181714625 1104 68-69 0.4509854067645662 1104 70-71 0.41529319229487527 1104 72-73 0.3898351738947028 1104 74-75 0.397393413430855 1104 76-77 0.40931435346050193 1104 78-79 0.39232766155507903 1104 80-81 0.3615044163209369 1104 82-83 0.37353599697679485 1104 84-85 0.37818387940433595 1104 86-87 0.3208742891497245 1104 88-89 0.36949747718940174 1104 90-91 0.3115874249022035 1104 92-93 0.35037644219419306 1104 94-95 0.438752838356784 1104 96-97 0.3574363975814592 1104 98-99 0.34808776593338564 1104 100 0.33636745209635777 1105 1 0.27030614621797966 1105 2 0.3053701111727847 1105 3 0.3023968491409761 1105 4 0.2566745821633063 1105 5 0.25384100094607476 1105 6 0.24458525628065786 1105 7 0.2602106503250283 1105 8 0.2555240380829815 1105 9 0.26390555930365167 1105 10-11 0.2668290014594561 1105 12-13 0.28850474920827907 1105 14-15 0.34570032793605066 1105 16-17 0.2969838146375281 1105 18-19 0.323408030850608 1105 20-21 0.3324476655760549 1105 22-23 0.3791935007561733 1105 24-25 0.399788142604514 1105 26-27 0.37338754123261 1105 28-29 0.35509392410069296 1105 30-31 0.3802862852775277 1105 32-33 0.3339554679193668 1105 34-35 0.3574769916753837 1105 36-37 0.34441734727958107 1105 38-39 0.35560451401643434 1105 40-41 0.3620612931356604 1105 42-43 0.3360800264117927 1105 44-45 0.3628803834202401 1105 46-47 0.3789284614587558 1105 48-49 0.39730743868586416 1105 50-51 0.3003416883219714 1105 52-53 0.3358806214956047 1105 54-55 0.43824966118097564 1105 56-57 0.42711501686689957 1105 58-59 0.3710134613879106 1105 60-61 0.3338703823569986 1105 62-63 0.3336754709086165 1105 64-65 0.314591226105037 1105 66-67 0.28726226529083476 1105 68-69 0.2911090047034648 1105 70-71 0.31013072937499686 1105 72-73 0.279546820152369 1105 74-75 0.25451983842302894 1105 76-77 0.26346894126415776 1105 78-79 0.2868567885471762 1105 80-81 0.23358783439954678 1105 82-83 0.23750317526971187 1105 84-85 0.2502200462479287 1105 86-87 0.19696712799093063 1105 88-89 0.17711207420492414 1105 90-91 0.19533656014187173 1105 92-93 0.21954418013530486 1105 94-95 0.2587839016189051 1105 96-97 0.24234112125762408 1105 98-99 0.2195837642309968 1105 100 0.2377146925727054 1106 1 0.21698902438233958 1106 2 0.26614199499976365 1106 3 0.25139250053153006 1106 4 0.2366733412883093 1106 5 0.24344051675829093 1106 6 0.2079447753635293 1106 7 0.2220410066883005 1106 8 0.23503873269316244 1106 9 0.2651204457769438 1106 10-11 0.24294560919484098 1106 12-13 0.2612315699612182 1106 14-15 0.33867900933380213 1106 16-17 0.3272486941976638 1106 18-19 0.2910790050381493 1106 20-21 0.2868572556086235 1106 22-23 0.2956515196335019 1106 24-25 0.29209817933153204 1106 26-27 0.3316154618240077 1106 28-29 0.3136118838145734 1106 30-31 0.3087463183168495 1106 32-33 0.2844833636727202 1106 34-35 0.3134631914607766 1106 36-37 0.29025213668932537 1106 38-39 0.3063148944083096 1106 40-41 0.2978901154569584 1106 42-43 0.34031341322191366 1106 44-45 0.3569480661663036 1106 46-47 0.36728458046195556 1106 48-49 0.37659405061021545 1106 50-51 0.24530478872622297 1106 52-53 0.2976173626743588 1106 54-55 0.38864830768219605 1106 56-57 0.3947008041640814 1106 58-59 0.33171517619908997 1106 60-61 0.31547579212001153 1106 62-63 0.3143220376601974 1106 64-65 0.31734506465117107 1106 66-67 0.293470112263833 1106 68-69 0.2988109555685199 1106 70-71 0.3374030560341126 1106 72-73 0.3245751178547138 1106 74-75 0.2994353633922415 1106 76-77 0.2999917804518972 1106 78-79 0.34329048915075333 1106 80-81 0.30649565546619684 1106 82-83 0.3249678622066021 1106 84-85 0.34195985058002165 1106 86-87 0.2593803001255601 1106 88-89 0.2667191246347862 1106 90-91 0.2588193860415622 1106 92-93 0.22610423838246874 1106 94-95 0.26958198454921245 1106 96-97 0.31734092608688513 1106 98-99 0.2829329187205545 1106 100 0.28635619125386214 1107 1 0.20226718877053784 1107 2 0.22644763200786144 1107 3 0.23135338945531458 1107 4 0.2194875597333379 1107 5 0.2282578401488422 1107 6 0.2069042905331031 1107 7 0.21598777508939548 1107 8 0.2031583133182977 1107 9 0.21279496278095422 1107 10-11 0.2068223471389885 1107 12-13 0.2127356826415081 1107 14-15 0.2625041893332707 1107 16-17 0.23469655407837564 1107 18-19 0.21707125461033172 1107 20-21 0.22888614145510644 1107 22-23 0.2715843002864986 1107 24-25 0.2852526695397586 1107 26-27 0.24339447149308313 1107 28-29 0.2658645165006206 1107 30-31 0.22385808394834328 1107 32-33 0.18557128177796756 1107 34-35 0.20255598973962918 1107 36-37 0.23552624712363723 1107 38-39 0.25373875543059654 1107 40-41 0.25964573635359756 1107 42-43 0.32474329665219415 1107 44-45 0.31602598173284235 1107 46-47 0.29695700714464834 1107 48-49 0.36904898198111624 1107 50-51 0.2622049420363055 1107 52-53 0.26055259457871216 1107 54-55 0.35200100383475075 1107 56-57 0.38532993910650504 1107 58-59 0.2972874838925179 1107 60-61 0.30685749502193715 1107 62-63 0.26897147683002487 1107 64-65 0.2667359740442379 1107 66-67 0.2639552000543972 1107 68-69 0.2704129421559287 1107 70-71 0.277496846415211 1107 72-73 0.2629315217743269 1107 74-75 0.26697072078959394 1107 76-77 0.26342188916613196 1107 78-79 0.26307665347628273 1107 80-81 0.20300230995714585 1107 82-83 0.24849120714585737 1107 84-85 0.24564258203720257 1107 86-87 0.22308900834552148 1107 88-89 0.21829956342225643 1107 90-91 0.24379491820765153 1107 92-93 0.22545444216126143 1107 94-95 0.2373350764283515 1107 96-97 0.267076404417125 1107 98-99 0.26164137847029423 1107 100 0.274716959206188 1108 1 0.09588566927095599 1108 2 0.1474431232153819 1108 3 0.15988810027783984 1108 4 0.14605214389678878 1108 5 0.14785688494165328 1108 6 0.0947847108723252 1108 7 0.11903093534894538 1108 8 0.14080525839869296 1108 9 0.15564839591280588 1108 10-11 0.10914763753251577 1108 12-13 0.1363900423696336 1108 14-15 0.17451482086926973 1108 16-17 0.13860151157312828 1108 18-19 0.12232500829836823 1108 20-21 0.08437641190546685 1108 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2316 88-89 -1.2715065611691188 2316 90-91 -1.1594648856920742 2316 92-93 -1.0639614883894097 2316 94-95 -1.2448597050618204 2316 96-97 -1.374805633633276 2316 98-99 -1.2260856576115344 2316 100 -1.0127025593843797 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18526.0 3 4548.0 4 3244.0 5 2698.0 6 4235.0 7 19333.0 8 38078.0 9 46491.0 10 48970.0 11 49034.0 12 52065.0 13 57855.0 14 67765.0 15 82125.0 16 102104.0 17 126140.0 18 137376.0 19 152179.0 20 174229.0 21 213167.0 22 276556.0 23 408655.0 24 603503.0 25 736778.0 26 720304.0 27 605917.0 28 528806.0 29 509667.0 30 562236.0 31 672498.0 32 834275.0 33 1074198.0 34 1477978.0 35 2252296.0 36 3886432.0 37 7219615.0 38 9123539.0 39 2680740.0 40 5954.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.563266873957268 35.1037306156077 27.21591564224915 21.11708686818588 2 20.691513667379567 34.05257584678189 26.767156013849018 18.488754471989527 3 26.80438106223563 25.535967690561268 26.273977761247743 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26.316375540132675 29.528040703857748 20.168053562019402 24-25 20.20316473044728 30.03348737950829 25.63610949206032 24.12723839798411 26-27 20.2356782436871 26.220721556911407 33.35123475806113 20.19236544134035 28-29 20.22608763490459 26.318319262182545 33.32979836665408 20.125794736258783 30-31 20.20290652873261 26.268379627914197 33.30630015546363 20.222413687889564 32-33 20.27119397130904 26.352258152406105 33.16677673530574 20.209771140979118 34-35 20.297072086652886 26.45004036117448 33.08198159060025 20.17090596157238 36-37 20.254852435062855 26.416210727506268 33.11259277740153 20.216344060029346 38-39 20.305329489658217 26.400249389117075 33.10587074990658 20.188550371318126 40-41 20.34292992485141 26.46231880930606 33.05606472723064 20.138686538611893 42-43 20.351902521255134 26.530580648862724 32.976904314136426 20.140612515745715 44-45 20.404258206996666 26.575281443290912 32.92945143216429 20.09100891754813 46-47 20.400080332581048 26.684521101796317 32.86761224117423 20.047786324448406 48-49 20.51813423257613 26.687490647402235 32.720813751923316 20.073561368098318 50-51 20.535178043264423 26.72922847337327 32.64552641449324 20.090067068869068 52-53 20.694668934535716 26.862990082956646 32.35722457157738 20.085116410930254 54-55 21.13834225763542 27.155110667427806 31.460182514285734 20.246364560651045 56-57 21.69006058715516 27.76098275216089 30.105835460587315 20.443121200096634 58-59 21.862953858806133 28.365831515608587 29.31885174519512 20.452362880390158 60-61 22.037204413402943 28.649514061732667 28.88199100275314 20.431290522111254 62-63 22.135620228089987 28.82145158069198 28.664171036149032 20.378757155068993 64-65 22.257934314458552 28.877737559388887 28.603711671804966 20.26061645434759 66-67 22.348237085433446 28.68739558520574 28.81440427202135 20.149963057339466 68-69 22.448695762195918 28.48541612951103 29.029308899991374 20.03657920830168 70-71 22.557993527508092 28.309245813986212 29.223626002532715 19.909134655972984 72-73 22.73403516212115 28.183359625850386 29.230514671677533 19.852090540350932 74-75 22.951888855945125 28.244693621231352 29.074853977643023 19.728563545180496 76-77 23.242340264258964 28.381995058683845 28.73693453112737 19.638730145929813 78-79 23.55971263308386 28.542370480632762 28.309827774025138 19.588089112258242 80-81 23.835634984726028 28.721619410771915 27.91382246246022 19.528923142041837 82-83 24.031209931026535 28.883706025754606 27.557686167989537 19.527397875229322 84-85 24.17593646547564 28.920316136280455 27.359375032095045 19.544372366148863 86-87 24.332500329927996 28.888876662819158 27.266998615716542 19.5116243915363 88-89 24.55728504438256 28.856765376865862 27.134449846901205 19.451499731850376 90-91 24.730192770202997 28.812651448847593 27.00158052058514 19.45557526036427 92-93 24.861078440678543 28.817226984033056 26.90629904156385 19.415395533724553 94-95 25.00581081178821 28.817091236091308 26.809712215729302 19.36738573639118 96-97 25.11537637412226 28.806151144006925 26.70386010207986 19.37461237979095 98-99 25.225865551490717 28.820196029088695 26.62893100963894 19.325007409781644 100 25.272260978844844 28.856848450873628 26.530693216298367 19.340197353983154 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 60055.0 1 40667.5 2 18006.0 3 13684.0 4 12497.0 5 12571.0 6 12786.0 7 12540.0 8 12463.0 9 12848.5 10 13648.5 11 17531.0 12 164592.0 13 205430.0 14 100034.0 15 104696.0 16 118605.0 17 131572.5 18 143855.5 19 156546.0 20 171089.0 21 190364.0 22 217009.0 23 248111.0 24 278642.5 25 313663.0 26 356178.0 27 406743.0 28 471912.5 29 535663.5 30 586179.5 31 637859.0 32 690874.5 33 736625.5 34 782562.5 35 836477.5 36 890167.0 37 938586.0 38 995686.0 39 1037520.5 40 1056415.0 41 1096524.5 42 1150211.0 43 1165375.0 44 1158067.5 45 1171304.0 46 1190529.5 47 1201392.5 48 1201954.0 49 1190103.5 50 1168986.0 51 1147465.5 52 1119681.0 53 1063527.5 54 990190.5 55 918363.0 56 844211.0 57 762205.0 58 672333.5 59 574188.0 60 471590.5 61 381303.0 62 301204.5 63 226883.0 64 167699.5 65 123038.0 66 89461.0 67 67169.5 68 51171.5 69 39107.5 70 30163.5 71 24235.5 72 20511.5 73 16764.0 74 14031.5 75 12561.0 76 10283.0 77 7098.5 78 4868.5 79 3734.5 80 3013.5 81 2446.5 82 2001.0 83 1682.5 84 1408.0 85 1190.5 86 973.0 87 789.5 88 683.5 89 573.5 90 509.5 91 448.5 92 393.0 93 348.0 94 300.0 95 253.5 96 210.5 97 161.5 98 119.5 99 94.0 100 64.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40253670948562864 2 0.3826660564755437 3 0.1674840287869832 4 0.17367850109734065 5 0.27383839661649156 6 0.10296483352538353 7 0.13666062686879346 8 0.1424025991601094 9 0.1353396640802871 10-11 0.13173512200313947 12-13 0.13393297923848407 14-15 0.13987590650720041 16-17 0.14392170636689167 18-19 0.1554632674115754 20-21 0.13671964861040758 22-23 0.0994094762329143 24-25 0.09480718566657567 26-27 0.0891734199015523 28-29 0.09898648708467982 30-31 0.10328242670644995 32-33 0.10437292364674881 34-35 0.10480012863367 36-37 0.10087939865501816 38-39 0.10021048558339155 40-41 0.10116888624483979 42-43 0.0641341486615457 44-45 0.06723700593497338 46-47 0.08543678154555401 48-49 0.08946150221181166 50-51 0.09879958490290179 52-53 0.1037222792094313 54-55 0.10607190663749794 56-57 0.10681389424636108 58-59 0.106060664401 60-61 0.10403003543356205 62-63 0.1082711691524048 64-65 0.11508818030883491 66-67 0.12354374743483781 68-69 0.12848471037567646 70-71 0.12678151154624062 72-73 0.11391055603567712 74-75 0.11170988824120802 76-77 0.11351145664000074 78-79 0.11233945348509192 80-81 0.10665228709670338 82-83 0.1097523338110066 84-85 0.11560391790817728 86-87 0.1288374355457989 88-89 0.1367070010943474 90-91 0.14331743615512813 92-93 0.14226066592432307 94-95 0.1326808751485275 96-97 0.12920421351154376 98-99 0.12454009064446654 100 0.11871801741810291 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.5580109E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.32950138577009 #Duplication Level Percentage of deduplicated Percentage of total 1 67.0116549463654 26.35534976075825 2 17.125057400087286 13.470399374962502 3 6.334046981760294 7.473447286400231 4 2.8901332706994087 4.546700019001306 5 1.6114327622825355 3.168842352863315 6 1.0231618653999515 2.4144267601868705 7 0.6994765502089683 1.925704476553011 8 0.49732981102427354 1.5647786793491154 9 0.3839552481227195 1.3590691616804575 >10 2.1290397965922714 15.098616862651104 >50 0.15323339506954256 4.160318468585994 >100 0.13710990686544414 10.076181911677343 >500 0.0037580680861100777 0.8761848436893713 >1k 5.100549972633793E-4 0.4739449792909748 >5k 7.138745637505733E-5 0.18434637897725747 >10k+ 2.8554982188416863E-5 6.851688683372828 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2332843 6.556593179633036 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 49895 0.14023284751600956 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 38803 0.10905812570725965 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0060989133001250785 8.431677373444808E-6 0.0 0.0 0.0 2 0.006110155536623005 1.4052795622408014E-5 0.0 5.621118248963206E-6 2.810559124481603E-6 3 0.006121397773120931 1.967391387137122E-5 0.0 1.1242236497926411E-5 2.810559124481603E-6 4 0.006132640009618858 3.0916150369297634E-5 0.0 1.1242236497926411E-5 2.810559124481603E-6 5 0.0061354505687433395 3.653726861826084E-5 0.0 1.1242236497926411E-5 5.621118248963206E-6 6 0.0061354505687433395 4.777950511618725E-5 0.0 1.6863354746889617E-5 5.621118248963206E-6 7 0.0061354505687433395 5.059006424066885E-5 0.0 1.967391387137122E-5 8.431677373444808E-6 8 0.00613826112786782 5.340062336515046E-5 0.0 2.5295032120334425E-5 8.431677373444808E-6 9 0.006141071686992302 5.6211182489632056E-5 0.0 2.8105591244816028E-5 1.4052795622408014E-5 10-11 0.006167771998674878 6.183230073859527E-5 0.0 3.513198905602004E-5 1.967391387137122E-5 12-13 0.006198688149044175 8.572205329668888E-5 0.0 3.794254818050164E-5 3.653726861826084E-5 14-15 0.006226793740288991 9.13431715456521E-5 0.0 4.2158386867224045E-5 3.653726861826084E-5 16-17 0.006266141568031734 1.082065262925417E-4 0.0 4.4968945991705645E-5 4.3563666429464845E-5 18-19 0.006318136911834644 1.1804348322822732E-4 0.0 4.7779505116187244E-5 4.918478467842805E-5 20-21 0.006371537535199794 1.3631211753735775E-4 0.0 5.199534380290965E-5 5.4805902927391256E-5 22-23 0.006520497168797318 1.5739131097096976E-4 0.0 5.340062336515046E-5 5.902174161411366E-5 24-25 0.007155683530930161 1.812810635290634E-4 0.0 5.902174161411366E-5 6.745341898755847E-5 26-27 0.009413967787451129 1.925233000269898E-4 0.0 5.902174161411366E-5 7.307453723652168E-5 28-29 0.016315295717615707 1.995496978381938E-4 0.0 6.183230073859527E-5 7.307453723652168E-5 30-31 0.03232424049066292 2.164130525850834E-4 0.0 6.323758030083606E-5 7.307453723652168E-5 32-33 0.06401469989875522 2.4030280514317703E-4 0.0 6.464285986307687E-5 7.588509636100328E-5 34-35 0.11942206247878555 2.5857143945230747E-4 0.0 7.026397811204007E-5 8.431677373444809E-5 36-37 0.20533101795725245 2.6981367595023387E-4 0.0 7.026397811204007E-5 8.993789198341129E-5 38-39 0.3255077155609613 2.7965063288591945E-4 0.0 7.447981679876249E-5 1.039906876058193E-4 40-41 0.48188160412886877 2.993245467572907E-4 0.0 7.588509636100328E-5 1.1523292410374573E-4 42-43 0.6774866822358526 3.077562241307355E-4 2.810559124481603E-6 7.588509636100328E-5 1.1944876279046813E-4 44-45 0.9176349066271832 3.1618790150418033E-4 2.810559124481603E-6 8.150621460996648E-5 1.2788044016391294E-4 46-47 1.1979867740146608 3.3726709493779236E-4 2.810559124481603E-6 8.150621460996648E-5 1.3490683797511693E-4 48-49 1.5259579446482303 3.5975156793364516E-4 2.810559124481603E-6 8.712733285892969E-5 1.3631211753735775E-4 50-51 1.8952471449708037 3.808307613672572E-4 2.810559124481603E-6 9.13431715456521E-5 1.3912267666183934E-4 52-53 2.2973861603403183 4.047205139253508E-4 2.810559124481603E-6 9.97748489190969E-5 1.4052795622408013E-4 54-55 2.7370812720107183 4.2720498692120365E-4 2.810559124481603E-6 1.011801284813377E-4 1.4333851534856175E-4 56-57 3.220066020595946 4.4547362123033404E-4 2.810559124481603E-6 1.0258540804357851E-4 1.4614907447304333E-4 58-59 3.745124839274663 4.679580942261869E-4 2.810559124481603E-6 1.0399068760581931E-4 1.5458075184648817E-4 60-61 4.304787261893998 4.918478467842805E-4 2.810559124481603E-6 1.1523292410374573E-4 1.5458075184648817E-4 62-63 4.892344483823813 5.03090083282207E-4 2.810559124481603E-6 1.1804348322822732E-4 1.5458075184648817E-4 64-65 5.501159369691644 5.171428789046149E-4 2.810559124481603E-6 1.2647516060167212E-4 1.5598603140872896E-4 66-67 6.136794016004842 5.227639971535781E-4 2.810559124481603E-6 1.3209627885063535E-4 1.5879659053321055E-4 68-69 6.803426318901947 5.283851154025414E-4 2.810559124481603E-6 1.3771739709959855E-4 1.6020187009545137E-4 70-71 7.495000928749263 5.368167927759862E-4 2.810559124481603E-6 1.4755435403528415E-4 1.6020187009545137E-4 72-73 8.201728668116221 5.550854270851165E-4 2.810559124481603E-6 1.5879659053321055E-4 1.6020187009545137E-4 74-75 8.924891151963589 5.691382227075246E-4 2.810559124481603E-6 1.6441770878217378E-4 1.6020187009545137E-4 76-77 9.664703388064382 5.789751796432103E-4 2.810559124481603E-6 1.77065224842341E-4 1.6020187009545137E-4 78-79 10.420888536344844 5.916226957033775E-4 2.810559124481603E-6 1.784705044045818E-4 1.6160714965769216E-4 80-81 11.192547217885139 6.056754913257855E-4 2.810559124481603E-6 1.7987578396682258E-4 1.6863354746889618E-4 82-83 11.97320952558071 6.281599643216383E-4 2.810559124481603E-6 1.7987578396682258E-4 1.7003882703113697E-4 84-85 12.764644706400421 6.506444373174911E-4 2.810559124481603E-6 1.812810635290634E-4 1.7565994528010017E-4 86-87 13.563262271062744 6.717236307511031E-4 4.215838686722404E-6 1.826863430913042E-4 1.77065224842341E-4 88 14.16586441598591 6.885869854979927E-4 5.621118248963206E-6 1.826863430913042E-4 1.77065224842341E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 293640 0.0 86.543884 8 AGTGGTA 295145 0.0 86.26661 5 TGGTATC 295310 0.0 85.828125 7 GTATCAA 296895 0.0 85.47503 9 GTGGTAT 299035 0.0 85.13335 6 AAGCAGT 302415 0.0 84.69312 1 GCAGTGG 304605 0.0 84.12587 3 CAGTGGT 308080 0.0 82.55843 4 AGCAGTG 314005 0.0 81.68633 2 CAACGCA 289900 0.0 44.85769 12-13 ATCAACG 292035 0.0 44.632645 10-11 ACGCAGA 292670 0.0 44.459858 14-15 AGAGTAC 292285 0.0 43.906643 18-19 TCAACGC 290025 0.0 43.897484 12-13 GAGTACT 284635 0.0 43.866055 20-21 AACGCAG 291845 0.0 43.731766 14-15 AGTACTT 294440 0.0 43.254616 20-21 CGCAGAG 294740 0.0 43.191475 16-17 TATCAAC 296150 0.0 42.757103 10-11 GTACTTT 295080 0.0 42.24821 22-23 >>END_MODULE