FastQCFastQC Report
Thu 26 May 2016
SRR1660707_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660707_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35580109
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21320155.992154211781645No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT476530.13393157395892183No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA415520.11678435274045956No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT397980.11185463203611883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2799850.085.690428
AAGCAGT2704550.085.371961
AGTGGTA2787200.085.3309565
TGGTATC2818250.085.0876857
GCAGTGG2801750.084.7999043
GTATCAA2841300.084.336149
GTGGTAT2822100.084.282356
AGCAGTG2882000.082.1027152
CAGTGGT2910850.081.54724
CAACGCA2753500.044.47570812-13
ATCAACG2771550.044.30297510-11
ACGCAGA2785950.044.05214314-15
GAGTACT2722500.043.63020720-21
AGAGTAC2812250.043.5760918-19
TCAACGC2751950.043.4679812-13
AACGCAG2766150.043.2975614-15
AGTACTT2831400.042.9526420-21
CGCAGAG2798150.042.8201416-17
TATCAAC2829750.042.27377710-11
GTACTTT2836500.041.81920222-23
Produced by FastQC (version 0.11.5)