FastQCFastQC Report
Thu 26 May 2016
SRR1660706_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660706_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25860438
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18740677.2468494153115275No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT376040.14541130355178053No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA304470.117735824892061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2330700.086.9653248
GTGGTAT2340850.086.550446
AGTGGTA2346000.086.3414465
TGGTATC2342900.086.107067
GTATCAA2351600.086.0896459
AAGCAGT2419200.084.175321
GCAGTGG2426200.084.02543
CAGTGGT2439200.082.901814
AGCAGTG2493750.081.83172
CAACGCA2303500.045.32207512-13
ATCAACG2316450.045.1336410-11
ACGCAGA2323100.044.92999614-15
AGAGTAC2313600.044.27711518-19
TCAACGC2302100.044.2316712-13
AACGCAG2320450.043.93282714-15
GAGTACT2272300.043.69096820-21
CGCAGAG2336500.043.53023516-17
TATCAAC2348100.043.0400110-11
AGTACTT2367800.042.92033420-21
GTACTTT2364000.041.94383222-23
Produced by FastQC (version 0.11.5)