##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660706_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 25860438 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.738873448315143 34.0 31.0 34.0 30.0 34.0 2 31.862374218101024 34.0 31.0 34.0 30.0 34.0 3 31.92727010269509 34.0 31.0 34.0 30.0 34.0 4 35.32658503309186 37.0 35.0 37.0 33.0 37.0 5 35.29051445300347 37.0 35.0 37.0 33.0 37.0 6 35.37528784315254 37.0 35.0 37.0 33.0 37.0 7 35.29978235480775 37.0 35.0 37.0 33.0 37.0 8 35.326167484092885 37.0 35.0 37.0 33.0 37.0 9 36.985299320916376 39.0 37.0 39.0 33.0 39.0 10-11 36.88863204869152 39.0 37.0 39.0 33.0 39.0 12-13 36.9543232794433 39.0 37.0 39.0 33.0 39.0 14-15 38.323310475251816 40.0 38.0 41.0 33.0 41.0 16-17 38.2947488360406 40.0 38.0 41.0 33.0 41.0 18-19 38.24703725049049 40.0 38.0 41.0 33.0 41.0 20-21 38.104573538158945 40.0 38.0 41.0 32.5 41.0 22-23 37.89586583954997 40.0 37.5 41.0 32.5 41.0 24-25 37.90571561471619 40.0 38.0 41.0 32.0 41.0 26-27 37.9256756014728 40.0 38.0 41.0 32.0 41.0 28-29 37.86054143785191 40.0 38.0 41.0 32.0 41.0 30-31 37.77334266341506 40.0 38.0 41.0 32.0 41.0 32-33 37.65274694883358 40.0 38.0 41.0 31.5 41.0 34-35 37.50206233939271 40.0 37.0 41.0 31.0 41.0 36-37 37.29910096650335 39.5 37.0 41.0 30.5 41.0 38-39 36.79152015909398 39.0 35.0 41.0 30.0 41.0 40-41 36.72613599970735 39.0 35.0 41.0 30.0 41.0 42-43 36.718607550266555 39.0 35.0 41.0 30.0 41.0 44-45 36.718375226281935 39.0 35.0 41.0 30.0 41.0 46-47 36.811913027149814 40.0 35.0 41.0 30.0 41.0 48-49 36.707478098398795 40.0 35.0 41.0 30.0 41.0 50-51 35.345264840448564 38.0 34.0 40.0 27.5 40.5 52-53 35.49116534685143 38.5 34.5 39.5 27.5 40.5 54-55 35.754780680822186 39.0 35.0 41.0 25.5 41.0 56-57 35.26677264708355 39.0 35.0 41.0 22.5 41.0 58-59 35.0660791978852 39.0 35.0 41.0 21.5 41.0 60-61 34.8691261532384 39.0 34.0 41.0 21.0 41.0 62-63 34.66885092974837 38.0 34.0 41.0 21.0 41.0 64-65 34.44800206013525 37.5 34.0 40.0 21.0 41.0 66-67 34.220414344876914 37.0 34.0 40.0 21.0 41.0 68-69 33.99181034752776 36.5 34.0 40.0 21.0 41.0 70-71 33.73859025512252 36.0 33.0 39.0 21.0 41.0 72-73 33.43012773024185 36.0 33.0 39.0 20.5 41.0 74-75 33.06916288502151 35.0 33.0 38.5 20.0 40.5 76-77 32.682447760552236 35.0 33.0 37.0 20.0 40.0 78-79 32.30919948068939 35.0 32.0 37.0 19.0 39.0 80-81 31.986229776927985 35.0 32.0 36.5 18.0 39.0 82-83 31.67231749903076 35.0 32.0 36.0 17.5 38.0 84-85 31.388850374460013 35.0 32.0 36.0 17.0 37.0 86-87 31.17919789293592 35.0 31.0 35.0 17.0 37.0 88-89 30.977517646839544 35.0 31.0 35.0 15.5 36.5 90-91 30.806524796679778 35.0 31.0 35.0 15.0 36.0 92-93 30.652327601721208 35.0 31.0 35.0 15.0 36.0 94-95 30.475610660577367 35.0 31.0 35.0 13.0 36.0 96-97 30.34388054448266 35.0 30.5 35.0 12.0 35.0 98-99 30.245837328818638 34.5 30.0 35.0 11.0 35.0 100 30.17279401841531 34.0 30.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5729246379384954 1101 2 0.595846290911819 1101 3 0.5873647113006477 1101 4 0.5065215958940072 1101 5 0.5106612200949101 1101 6 0.507935158444127 1101 7 0.5336972092170953 1101 8 0.5466315097244916 1101 9 0.6346894455665861 1101 10-11 0.6473036944379302 1101 12-13 0.6273864192832193 1101 14-15 0.7344394484100718 1101 16-17 0.6966347416721632 1101 18-19 0.7114462207713004 1101 20-21 0.7434756895368579 1101 22-23 0.8032007765307583 1101 24-25 0.811021767420911 1101 26-27 0.746086952342381 1101 28-29 0.7172509672051888 1101 30-31 0.7033166584690065 1101 32-33 0.690233652829761 1101 34-35 0.7010629001343105 1101 36-37 0.6975356837542392 1101 38-39 0.69697011920492 1101 40-41 0.68637333980449 1101 42-43 0.7212214879927714 1101 44-45 0.7587422570149585 1101 46-47 0.7872013460253342 1101 48-49 0.804072440776352 1101 50-51 0.7539183125355038 1101 52-53 0.8213885021460428 1101 54-55 0.8228170364558949 1101 56-57 0.7651368558451566 1101 58-59 0.6672539803563708 1101 60-61 0.6666017543808209 1101 62-63 0.6352073704706598 1101 64-65 0.6137296420412781 1101 66-67 0.6001572369342725 1101 68-69 0.608725406005469 1101 70-71 0.5963162376832756 1101 72-73 0.5709950907906816 1101 74-75 0.4941953985779364 1101 76-77 0.5334727762077236 1101 78-79 0.558868126991058 1101 80-81 0.5331464910046542 1101 82-83 0.5139776457617806 1101 84-85 0.4644992274183508 1101 86-87 0.43966710257741326 1101 88-89 0.43921255265355086 1101 90-91 0.4156823919298134 1101 92-93 0.3920035990082553 1101 94-95 0.46861763732214 1101 96-97 0.46664686111884635 1101 98-99 0.4086267949969198 1101 100 0.3604570216393519 1102 1 0.4795781842074298 1102 2 0.5150412045313111 1102 3 0.5228417137929178 1102 4 0.48976928495505234 1102 5 0.44499160720611997 1102 6 0.41776742246986487 1102 7 0.4455457964193599 1102 8 0.49126088179723837 1102 9 0.5046475091617708 1102 10-11 0.5231380967573571 1102 12-13 0.5407267773823037 1102 14-15 0.6377212759036937 1102 16-17 0.6039647198359077 1102 18-19 0.5641017317241435 1102 20-21 0.6330644031656121 1102 22-23 0.6456474879758929 1102 24-25 0.5981579343872099 1102 26-27 0.5960754072925738 1102 28-29 0.6047927058877605 1102 30-31 0.5812251574389151 1102 32-33 0.6124987925663419 1102 34-35 0.5698075664563405 1102 36-37 0.5879740725398221 1102 38-39 0.6392792593735876 1102 40-41 0.604398345398586 1102 42-43 0.5884070035366307 1102 44-45 0.6316731323554237 1102 46-47 0.6500862437113071 1102 48-49 0.6827511498526704 1102 50-51 0.6176217465367841 1102 52-53 0.6461101622798395 1102 54-55 0.6851977954778121 1102 56-57 0.7143036253916932 1102 58-59 0.5907595763780051 1102 60-61 0.5593941759284888 1102 62-63 0.5847342115058396 1102 64-65 0.5600308521013844 1102 66-67 0.5369645628808328 1102 68-69 0.5148763664533007 1102 70-71 0.4878733292566366 1102 72-73 0.4607146772522057 1102 74-75 0.4756449967677625 1102 76-77 0.4987033058718495 1102 78-79 0.4821261988809411 1102 80-81 0.4000988438028621 1102 82-83 0.3623305326993851 1102 84-85 0.38991250547947587 1102 86-87 0.3273092214653808 1102 88-89 0.3518409853045341 1102 90-91 0.3435879829360502 1102 92-93 0.3483899887737678 1102 94-95 0.3642973726251064 1102 96-97 0.33634016981245196 1102 98-99 0.2842766501292111 1102 100 0.2992923797770217 1103 1 0.40775782117084347 1103 2 0.44101066030210134 1103 3 0.4651576950165115 1103 4 0.3949659273623425 1103 5 0.4320290290253723 1103 6 0.3932840215403104 1103 7 0.39335449729810534 1103 8 0.4257172189069891 1103 9 0.5186563632538039 1103 10-11 0.46844115346002724 1103 12-13 0.49071209836304064 1103 14-15 0.5797628926331058 1103 16-17 0.532844724959908 1103 18-19 0.514820885157441 1103 20-21 0.5623476507009499 1103 22-23 0.6081241604034346 1103 24-25 0.6225139202354271 1103 26-27 0.5949278618894809 1103 28-29 0.5947853663781331 1103 30-31 0.5591607830730894 1103 32-33 0.5148154423339548 1103 34-35 0.5320154986632559 1103 36-37 0.5033437068181996 1103 38-39 0.536869518243968 1103 40-41 0.48130296488395885 1103 42-43 0.5184527721143724 1103 44-45 0.5477406053639768 1103 46-47 0.5763318063521652 1103 48-49 0.5858704360177285 1103 50-51 0.5618530873737342 1103 52-53 0.577526881235876 1103 54-55 0.6574652706213655 1103 56-57 0.6790742867490849 1103 58-59 0.5663563754001046 1103 60-61 0.5972975323270475 1103 62-63 0.5547758629742034 1103 64-65 0.5683667266257189 1103 66-67 0.5134717071647472 1103 68-69 0.5334058636285945 1103 70-71 0.4799342876248929 1103 72-73 0.4744597622323923 1103 74-75 0.48534764847206446 1103 76-77 0.4630273282596633 1103 78-79 0.4485114826982297 1103 80-81 0.403963400041615 1103 82-83 0.39488668677824634 1103 84-85 0.41691874727420597 1103 86-87 0.38712591737067115 1103 88-89 0.3757347991645119 1103 90-91 0.3512952587214073 1103 92-93 0.3125839978956364 1103 94-95 0.3746318680851566 1103 96-97 0.3265688136758875 1103 98-99 0.32813640804933186 1103 100 0.30488219120237403 1104 1 0.3736119253220984 1104 2 0.40485275045005764 1104 3 0.40787406112236724 1104 4 0.35361520728214657 1104 5 0.3649397974263877 1104 6 0.2717640638371037 1104 7 0.33196585155302216 1104 8 0.32917615502968545 1104 9 0.3577771343455183 1104 10-11 0.3632251047908355 1104 12-13 0.3696247235936383 1104 14-15 0.4546073601990912 1104 16-17 0.4352065645603034 1104 18-19 0.4278330616360151 1104 20-21 0.38848620395295086 1104 22-23 0.4115334787321956 1104 24-25 0.4468167320114276 1104 26-27 0.3821809955604891 1104 28-29 0.35995113707737403 1104 30-31 0.346599318219468 1104 32-33 0.3153157102266704 1104 34-35 0.3533941783964849 1104 36-37 0.33795839991472576 1104 38-39 0.357790173964851 1104 40-41 0.35069292827387955 1104 42-43 0.3497535032439032 1104 44-45 0.37338396509558436 1104 46-47 0.3801458516333085 1104 48-49 0.4519371008416897 1104 50-51 0.4132574772087665 1104 52-53 0.42217109659679153 1104 54-55 0.5629474185134953 1104 56-57 0.6267629455361856 1104 58-59 0.5556431895213549 1104 60-61 0.5620969776438471 1104 62-63 0.552541647144615 1104 64-65 0.4858057199883774 1104 66-67 0.49801043003214573 1104 68-69 0.4860049735876899 1104 70-71 0.4751283156101138 1104 72-73 0.46223033267874314 1104 74-75 0.4221059800830531 1104 76-77 0.45532662602898455 1104 78-79 0.44922646314204684 1104 80-81 0.40778468387872735 1104 82-83 0.3837730038724949 1104 84-85 0.39239723912645275 1104 86-87 0.34354314256822605 1104 88-89 0.3187406152196388 1104 90-91 0.3220440402683238 1104 92-93 0.3416611510734029 1104 94-95 0.4133946352112936 1104 96-97 0.338189257025288 1104 98-99 0.38883467613483447 1104 100 0.28344929766899796 1105 1 0.3103390683957876 1105 2 0.3569546309131404 1105 3 0.3344360377344451 1105 4 0.2980151929700696 1105 5 0.33153783051978536 1105 6 0.29979490303089307 1105 7 0.3225545140819577 1105 8 0.30478173200409486 1105 9 0.348005743598641 1105 10-11 0.3213684865407842 1105 12-13 0.3135909193151818 1105 14-15 0.34357272650281345 1105 16-17 0.3233418018518037 1105 18-19 0.32681806589426543 1105 20-21 0.3487802196914416 1105 22-23 0.39709394354657945 1105 24-25 0.43325489623178015 1105 26-27 0.43633493795719147 1105 28-29 0.42492798911703744 1105 30-31 0.4045579970866484 1105 32-33 0.4120475909554955 1105 34-35 0.41806467245595513 1105 36-37 0.392842364334129 1105 38-39 0.3902693795722385 1105 40-41 0.3926361063206727 1105 42-43 0.41466668887992597 1105 44-45 0.4492324147488276 1105 46-47 0.4537280053477133 1105 48-49 0.489080316820953 1105 50-51 0.35577583756570164 1105 52-53 0.4323152072971368 1105 54-55 0.5496090997229075 1105 56-57 0.542208630834466 1105 58-59 0.4468826715767591 1105 60-61 0.4340856423437529 1105 62-63 0.4332194745210387 1105 64-65 0.4417110935571813 1105 66-67 0.41508350804740957 1105 68-69 0.4495098949503742 1105 70-71 0.4277415391019943 1105 72-73 0.42769423730236156 1105 74-75 0.4167942379544911 1105 76-77 0.42317295863360016 1105 78-79 0.4180196978354047 1105 80-81 0.3844619219357348 1105 82-83 0.3585561716622898 1105 84-85 0.3384382316497394 1105 86-87 0.28886789248536004 1105 88-89 0.3426071500793171 1105 90-91 0.31128168297279046 1105 92-93 0.33249762942209315 1105 94-95 0.36790781439149 1105 96-97 0.3200043709534519 1105 98-99 0.29366692166027875 1105 100 0.3163683536380333 1106 1 0.21845463433118084 1106 2 0.2797607194111329 1106 3 0.25667501043536944 1106 4 0.2500390046367045 1106 5 0.25294817520767054 1106 6 0.22638013185806471 1106 7 0.2361281557765338 1106 8 0.24666420632475905 1106 9 0.2466002980024058 1106 10-11 0.23100244139148174 1106 12-13 0.23954367153244505 1106 14-15 0.3074081650838849 1106 16-17 0.2540876509704546 1106 18-19 0.2295778167736131 1106 20-21 0.2732292494641655 1106 22-23 0.266735998059211 1106 24-25 0.2817794787731742 1106 26-27 0.2830149290435955 1106 28-29 0.24108159405395213 1106 30-31 0.2392859317166085 1106 32-33 0.27475323466479296 1106 34-35 0.2684017886429757 1106 36-37 0.21429482340205652 1106 38-39 0.23248898195716805 1106 40-41 0.25460559051369813 1106 42-43 0.26932500942051263 1106 44-45 0.3132094338143858 1106 46-47 0.32730632146262195 1106 48-49 0.32202205615082846 1106 50-51 0.19640071565640937 1106 52-53 0.27948943009659644 1106 54-55 0.4105723176624636 1106 56-57 0.4008139722206039 1106 58-59 0.3636398523601514 1106 60-61 0.3605210655089479 1106 62-63 0.35921359706697586 1106 64-65 0.3761486857048979 1106 66-67 0.35025639382258333 1106 68-69 0.34053958334312284 1106 70-71 0.32541892184774923 1106 72-73 0.32401655232309423 1106 74-75 0.30796925160928623 1106 76-77 0.31335561389514766 1106 78-79 0.33139283569456524 1106 80-81 0.31773278637162505 1106 82-83 0.30061625127364167 1106 84-85 0.3220706548580665 1106 86-87 0.24704170323668606 1106 88-89 0.22494118143398012 1106 90-91 0.23169718500942338 1106 92-93 0.22492862093766064 1106 94-95 0.245552988831804 1106 96-97 0.25547631633582313 1106 98-99 0.25988415524925657 1106 100 0.2611246807623395 1107 1 0.2104022553426752 1107 2 0.2449864280793328 1107 3 0.22307006174500899 1107 4 0.22119695395750938 1107 5 0.23223282716915605 1107 6 0.17554816154221697 1107 7 0.19596635788123962 1107 8 0.2233406032581371 1107 9 0.2193692042009303 1107 10-11 0.22979889331832482 1107 12-13 0.2055474775088726 1107 14-15 0.21946176075529422 1107 16-17 0.22116047703482877 1107 18-19 0.2154789063271707 1107 20-21 0.21253186850919548 1107 22-23 0.24100588122164623 1107 24-25 0.2632589744048559 1107 26-27 0.229051060649077 1107 28-29 0.22908072014056557 1107 30-31 0.18674788247960095 1107 32-33 0.14851569590170755 1107 34-35 0.2247994592552658 1107 36-37 0.21749822812913777 1107 38-39 0.22210001657251865 1107 40-41 0.24215896443362794 1107 42-43 0.2871035945447602 1107 44-45 0.26677241630063264 1107 46-47 0.27225578583818333 1107 48-49 0.34551322496443504 1107 50-51 0.24554280555253172 1107 52-53 0.2530886941317405 1107 54-55 0.3568553603777005 1107 56-57 0.39762150637160687 1107 58-59 0.32796216762548625 1107 60-61 0.34058304265690253 1107 62-63 0.335154429070208 1107 64-65 0.30415567356735806 1107 66-67 0.33502024436051414 1107 68-69 0.3038965617740885 1107 70-71 0.29562376713655425 1107 72-73 0.2671452543431414 1107 74-75 0.2856331798636802 1107 76-77 0.2748004714733838 1107 78-79 0.27246286479530823 1107 80-81 0.22040383212172898 1107 82-83 0.2310585476481144 1107 84-85 0.2473563192915691 1107 86-87 0.23364961041591314 1107 88-89 0.2559589654049823 1107 90-91 0.25372755492266563 1107 92-93 0.22709190978692817 1107 94-95 0.2552205407163619 1107 96-97 0.30121778131979937 1107 98-99 0.29259819365834616 1107 100 0.27645465615420406 1108 1 0.12784881142510685 1108 2 0.16554395330413385 1108 3 0.17175446373326864 1108 4 0.17983614511027923 1108 5 0.2028331352496977 1108 6 0.10892877898798758 1108 7 0.14085570596112973 1108 8 0.14472176571933204 1108 9 0.18214110043473397 1108 10-11 0.1486321049148458 1108 12-13 0.1539520866509747 1108 14-15 0.19583794455854786 1108 16-17 0.14475971925214282 1108 18-19 0.13602590125464786 1108 20-21 0.16097579585179034 1108 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#Quality Count 2 13128.0 3 3338.0 4 2452.0 5 2031.0 6 3057.0 7 12454.0 8 24396.0 9 29039.0 10 31506.0 11 33927.0 12 39170.0 13 46257.0 14 55800.0 15 64968.0 16 74972.0 17 86922.0 18 98998.0 19 112647.0 20 130092.0 21 156901.0 22 201577.0 23 288379.0 24 444841.0 25 581622.0 26 570944.0 27 462206.0 28 393641.0 29 372120.0 30 402197.0 31 479266.0 32 592586.0 33 760804.0 34 1044886.0 35 1601397.0 36 2752491.0 37 5077874.0 38 6606231.0 39 2200706.0 40 4615.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 15.785612861575835 35.53833919793988 27.143085722264082 21.532962218220206 2 20.385434844262683 34.87799600619203 26.856716607252284 17.87985254229301 3 27.337169286366475 25.272764134363353 26.278088492745166 21.111978086525006 4 18.878837495454174 27.060009256675333 24.73668678890829 29.3244664589622 5 20.914243516702776 34.229934106493324 25.706067777108927 19.14975459969497 6 29.32361441822725 26.309434125278674 25.53204452640011 18.834906930093968 7 20.31919302187676 25.546090372027226 34.118145801550426 20.01657080454559 8 28.459246781974212 25.930056240592155 25.597247222580314 20.01344975485332 9 27.858445431377486 25.004473337803123 26.876920965094797 20.260160265724593 10-11 19.82632346805401 30.36659657030484 29.94985172856722 19.85722823307393 12-13 19.78433577834383 26.170649837510418 29.879190677673396 24.165823706472356 14-15 19.754326533578162 34.63324136911667 25.728212795592597 19.884219301712573 16-17 24.121584750161478 25.950118053118686 25.78257496507123 24.145722231648605 18-19 19.855508624054625 30.245665780603524 25.769175959343325 24.12964963599852 20-21 24.117486191474157 30.3080949127839 25.743156046675946 19.83126284906599 22-23 24.10694047307177 26.078519921272374 29.954231284693382 19.86030832096247 24-25 19.912267017323014 30.100990584054966 25.699041166066667 24.28770123255536 26-27 19.94669423275635 25.871576715772733 34.27007685173768 19.91165219973324 28-29 19.924474126136957 25.9726481917549 34.2518779377705 19.850999744337642 30-31 19.905315821924194 25.893268289540877 34.26478047720045 19.93663541133448 32-33 20.003817539052598 25.994119665991267 34.0869676196082 19.915095175347926 34-35 20.018461136831473 26.10476399988898 34.00498552501478 19.87178933826477 36-37 19.969826241349775 26.050973531634806 34.03455942807349 19.944640798941922 38-39 19.983119850608993 26.064639874598633 34.04546411159626 19.90677616319612 40-41 19.98218022486879 26.10033656715131 34.03650983005274 19.88097337792716 42-43 19.995241474745836 26.11447936856405 33.97936627146233 19.91091288522778 44-45 19.977388018366096 26.171943811448646 33.96886362980753 19.881804540377722 46-47 19.9573980250807 26.258422348363574 33.9239854862169 19.860194140338823 48-49 20.017268043491505 26.253177477139467 33.81489575428909 19.914658725079946 50-51 20.017886693544444 26.311823594081595 33.7618811181293 19.908408594244655 52-53 20.116868891659326 26.446500419987963 33.468409993644485 19.968220694708226 54-55 20.508636963031392 26.80508336957593 32.46918411875725 20.21709554863543 56-57 21.06532479606415 27.516299161126994 30.97078394203129 20.447592100777563 58-59 21.120869466674165 28.186019756436064 30.1681067098778 20.52500406701197 60-61 21.1085552654248 28.555550714702495 29.794653400157234 20.541240619715474 62-63 21.03063947968747 28.77033696279613 29.641134344370823 20.55788921314558 64-65 21.001227351934485 28.804559680379793 29.6697376855115 20.524475282174222 66-67 20.96545392372329 28.524653235234766 29.99563422452042 20.514258616521523 68-69 20.90594663737199 28.25835826741592 30.377501029552135 20.45819406565995 70-71 20.891536198259285 28.10997594612306 30.632029269728676 20.366458585888974 72-73 20.908898748405722 28.006759797612563 30.705758332492923 20.378583121488788 74-75 20.975806549019158 28.112574177485843 30.582551671445884 20.32906760204911 76-77 21.146222657516205 28.299178202595897 30.23301158201759 20.32158755787031 78-79 21.304221268386623 28.49510819225752 29.839525896373313 20.361144642982545 80-81 21.466422747984886 28.740588510208376 29.45299114264416 20.33999759916258 82-83 21.57423850809737 28.96772276312053 29.076355998723823 20.381682730058277 84-85 21.616014502326962 29.061571495145973 28.895876796034898 20.426537206492174 86-87 21.681108991844656 29.15557878550886 28.736525846776615 20.426786375869867 88-89 21.808463563521258 29.248012836708064 28.556300744680783 20.38722285508989 90-91 21.9329722483704 29.257452953802737 28.3806135604515 20.42896123737536 92-93 22.02828111687384 29.316183050843385 28.268897209465088 20.386638622817685 94-95 22.131246025767624 29.394433275140745 28.132572144490563 20.341748554601065 96-97 22.179748693545502 29.380466695392784 28.069475224152455 20.37030938690926 98-99 22.26906062840663 29.38859666858268 28.011985941108858 20.33035676190183 100 22.290032365744725 29.397828503559246 27.952096111615766 20.36004301908026 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 43540.0 1 31115.0 2 15600.0 3 11708.0 4 10666.5 5 10173.0 6 9959.0 7 9800.0 8 9621.5 9 9740.5 10 10155.5 11 13851.0 12 145845.5 13 181964.0 14 87250.0 15 91407.0 16 103524.0 17 114526.0 18 124316.0 19 133583.0 20 144823.0 21 160209.5 22 180815.0 23 205317.5 24 233524.0 25 266623.0 26 304618.0 27 349195.5 28 401079.5 29 450647.5 30 489853.5 31 523587.5 32 561257.5 33 599560.0 34 630294.0 35 660519.5 36 699326.5 37 735709.0 38 760434.0 39 774592.5 40 777593.5 41 782773.5 42 797210.5 43 800521.5 44 801778.5 45 807809.5 46 804307.5 47 799413.5 48 785623.5 49 763612.5 50 747995.0 51 733709.0 52 714048.0 53 682454.0 54 639375.0 55 597525.5 56 557704.5 57 513525.0 58 461519.0 59 402655.0 60 338771.5 61 277701.5 62 223092.5 63 172492.0 64 129818.0 65 96767.5 66 72688.0 67 56342.0 68 44287.0 69 35358.5 70 29018.5 71 24440.5 72 21043.5 73 17898.5 74 14954.5 75 12581.5 76 10255.0 77 7753.5 78 5800.0 79 4338.0 80 3259.5 81 2442.5 82 1863.0 83 1428.0 84 1092.0 85 834.5 86 703.5 87 620.5 88 535.5 89 443.5 90 375.0 91 329.5 92 289.0 93 246.5 94 196.0 95 157.0 96 143.0 97 123.0 98 98.0 99 74.0 100 48.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40636202681485906 2 0.3849896123182446 3 0.17012086183536412 4 0.1761532422614033 5 0.2766929160287231 6 0.1021483085475969 7 0.13727532379768664 8 0.1427624698390646 9 0.1361268513704215 10-11 0.13272590355971542 12-13 0.13449888203749683 14-15 0.14092762079281101 16-17 0.14511935180680235 18-19 0.1562985901476224 20-21 0.13673588977881967 22-23 0.09876862874480317 24-25 0.09402972989088584 26-27 0.08853678348371362 28-29 0.09892717207651318 30-31 0.10350172723292622 32-33 0.10456706108380685 34-35 0.10537717883973968 36-37 0.1008084240491209 38-39 0.1003501951513737 40-41 0.10138072680748872 42-43 0.0630171074441972 44-45 0.06596756017821508 46-47 0.08493475632547291 48-49 0.08938750380020632 50-51 0.0989561739054845 52-53 0.10424997442038686 54-55 0.10662232403024265 56-57 0.1072816322755245 58-59 0.10668999496450911 60-61 0.10466373384704465 62-63 0.10902560892433454 64-65 0.11612719011178388 66-67 0.12476973514524387 68-69 0.13021434517079716 70-71 0.1284142983193092 72-73 0.11516819630046483 74-75 0.11272044193528354 76-77 0.11453402297362482 78-79 0.11326954323047429 80-81 0.10744210906249924 82-83 0.11045636582025409 84-85 0.11639594039358499 86-87 0.1300422676522339 88-89 0.13823625106427045 90-91 0.14509421688836052 92-93 0.1442608976692506 94-95 0.13416632773195875 96-97 0.13056043366318854 98-99 0.1257209951355039 100 0.1197543521884664 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.5860438E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.97104596929916 #Duplication Level Percentage of deduplicated Percentage of total 1 64.6947853646145 25.212234544183342 2 17.672515368088078 13.774328176058127 3 7.065863350001305 8.260922562770608 4 3.226446383887458 5.029519613758287 5 1.8200730072659592 3.5465074416821127 6 1.1358396962671622 2.6558916610189427 7 0.7677936316883796 2.094520464082409 8 0.5590139386417393 1.7428286320228963 9 0.4319963629069035 1.515181510567348 >10 2.3658033668658365 17.3578167705076 >50 0.16854498355088696 4.468489543620093 >100 0.08994707806816378 5.931455955232582 >500 6.442443332743367E-4 0.16836820563779223 >1k 6.540437743048432E-4 0.598919219902078 >5k 3.959002306537293E-5 0.10840166741468331 >10k+ 3.959002306527782E-5 7.534614031540982 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1874067 7.2468494153115275 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 37604 0.14541130355178053 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 30447 0.117735824892061 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0015196958380983337 3.8669105295123E-6 0.0 0.0 0.0 2 0.0015235627486278461 3.8669105295123E-6 0.0 0.0 0.0 3 0.0015235627486278461 7.7338210590246E-6 0.0 0.0 3.8669105295123E-6 4 0.0015235627486278461 1.9334552647561498E-5 0.0 0.0 3.8669105295123E-6 5 0.0015235627486278461 2.32014631770738E-5 0.0 0.0 7.7338210590246E-6 6 0.0015274296591573585 2.32014631770738E-5 0.0 0.0 7.7338210590246E-6 7 0.0015274296591573585 3.09352842360984E-5 0.0 0.0 7.7338210590246E-6 8 0.0015274296591573585 3.09352842360984E-5 0.0 3.8669105295123E-6 7.7338210590246E-6 9 0.0015274296591573585 3.48021947656107E-5 0.0 3.8669105295123E-6 7.7338210590246E-6 10-11 0.0015390303907458955 3.8669105295122995E-5 0.0 7.7338210590246E-6 7.7338210590246E-6 12-13 0.0015428973012754077 4.833638161890375E-5 0.0 7.7338210590246E-6 1.740109738280535E-5 14-15 0.0015506311223344323 6.57374790017091E-5 0.0 7.7338210590246E-6 1.9334552647561498E-5 16-17 0.001562231853922969 7.34713000607337E-5 0.0 2.32014631770738E-5 1.9334552647561498E-5 18-19 0.001569965674981994 7.34713000607337E-5 0.0 2.32014631770738E-5 1.9334552647561498E-5 20-21 0.0015796329513057745 7.733821059024599E-5 0.0 2.70683737065861E-5 1.9334552647561498E-5 22-23 0.0016028344144828482 8.89389421787829E-5 0.0 2.70683737065861E-5 1.9334552647561498E-5 24-25 0.0016821060803378504 9.66727632378075E-5 0.0 2.900182897134225E-5 1.9334552647561498E-5 26-27 0.0018967196147257831 1.0053967376731979E-4 0.0 3.48021947656107E-5 1.9334552647561498E-5 28-29 0.0026585009890397063 1.1407386062061285E-4 0.0 3.48021947656107E-5 1.9334552647561498E-5 30-31 0.004334806703583288 1.4114223432719895E-4 0.0 3.8669105295122995E-5 2.126800791231765E-5 32-33 0.007743488335348381 1.430756895919551E-4 0.0 5.41367474131722E-5 2.32014631770738E-5 34-35 0.013648260713913663 1.4887605538622354E-4 0.0 5.41367474131722E-5 2.32014631770738E-5 36-37 0.022921112163684157 1.585433317100043E-4 0.0 5.80036579426845E-5 2.32014631770738E-5 38-39 0.036706648201395506 1.7787788435756578E-4 1.93345526475615E-6 6.18705684721968E-5 2.5134918441829947E-5 40-41 0.05496426626648783 1.894786159461027E-4 3.8669105295123E-6 6.18705684721968E-5 2.900182897134225E-5 42-43 0.07837647606742004 1.9914589226988343E-4 3.8669105295123E-6 6.18705684721968E-5 3.09352842360984E-5 44-45 0.1076470553205634 2.1848044491744494E-4 3.8669105295123E-6 6.380402373695296E-5 3.09352842360984E-5 46-47 0.14485446843553074 2.3394808703549414E-4 3.8669105295123E-6 6.57374790017091E-5 3.673565003036685E-5 48-49 0.19164601929789432 2.4361536335927488E-4 3.8669105295123E-6 6.57374790017091E-5 4.25360158246353E-5 50-51 0.2455198167950597 2.494157291535433E-4 5.80036579426845E-6 6.57374790017091E-5 5.41367474131722E-5 52-53 0.3068664188905076 2.6488337127159256E-4 7.7338210590246E-6 6.96043895312214E-5 5.993711320744065E-5 54-55 0.3763045312689599 2.70683737065861E-4 7.7338210590246E-6 7.733821059024599E-5 7.153784479597755E-5 56-57 0.4583333043315044 2.8035101338964174E-4 7.7338210590246E-6 7.733821059024599E-5 7.540475532548984E-5 58-59 0.5529024682412571 2.938852002429348E-4 7.7338210590246E-6 8.50720316492706E-5 7.733821059024599E-5 60-61 0.6596291988557966 3.0935284236098396E-4 7.7338210590246E-6 9.087239744353905E-5 7.733821059024599E-5 62-63 0.7784864278014162 3.2288702921427705E-4 7.7338210590246E-6 9.28058527082952E-5 7.733821059024599E-5 64-65 0.9079834610689889 3.286873950085455E-4 7.7338210590246E-6 9.28058527082952E-5 8.893894217878289E-5 66-67 1.051003080458266 3.325543055380578E-4 1.16007315885369E-5 9.28058527082952E-5 8.893894217878289E-5 68-69 1.209832563547454 3.402881265970824E-4 1.54676421180492E-5 9.473930797305135E-5 8.893894217878289E-5 70-71 1.383445245590968 3.460884923913508E-4 1.54676421180492E-5 9.860621850256364E-5 8.893894217878289E-5 72-73 1.570456772619242 3.634895897741562E-4 1.54676421180492E-5 1.0440658429683209E-4 8.893894217878289E-5 74-75 1.7705925939846803 3.6542304503891235E-4 1.54676421180492E-5 1.082734948263444E-4 8.893894217878289E-5 76-77 1.9859505086495441 3.712234108331808E-4 1.54676421180492E-5 1.1020695009110054E-4 9.087239744353905E-5 78-79 2.218980204434279 3.750903213626931E-4 1.54676421180492E-5 1.1214040535585669E-4 9.473930797305135E-5 80-81 2.4678719672110736 3.828241424217177E-4 1.54676421180492E-5 1.1407386062061285E-4 1.0053967376731979E-4 82-83 2.736626115922708 3.9829178453976686E-4 1.54676421180492E-5 1.16007315885369E-4 1.0053967376731979E-4 84-85 3.0225261459221997 4.1569288192257225E-4 1.54676421180492E-5 1.198742264148813E-4 1.082734948263444E-4 86-87 3.32512929595392 4.350274345701337E-4 1.54676421180492E-5 1.198742264148813E-4 1.082734948263444E-4 88 3.5634431249772334 4.4856162142342676E-4 1.54676421180492E-5 1.198742264148813E-4 1.082734948263444E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 233070 0.0 86.965324 8 GTGGTAT 234085 0.0 86.55044 6 AGTGGTA 234600 0.0 86.341446 5 TGGTATC 234290 0.0 86.10706 7 GTATCAA 235160 0.0 86.089645 9 AAGCAGT 241920 0.0 84.17532 1 GCAGTGG 242620 0.0 84.0254 3 CAGTGGT 243920 0.0 82.90181 4 AGCAGTG 249375 0.0 81.8317 2 CAACGCA 230350 0.0 45.322075 12-13 ATCAACG 231645 0.0 45.13364 10-11 ACGCAGA 232310 0.0 44.929996 14-15 AGAGTAC 231360 0.0 44.277115 18-19 TCAACGC 230210 0.0 44.23167 12-13 AACGCAG 232045 0.0 43.932827 14-15 GAGTACT 227230 0.0 43.690968 20-21 CGCAGAG 233650 0.0 43.530235 16-17 TATCAAC 234810 0.0 43.04001 10-11 AGTACTT 236780 0.0 42.920334 20-21 GTACTTT 236400 0.0 41.943832 22-23 >>END_MODULE