FastQCFastQC Report
Thu 26 May 2016
SRR1660706_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660706_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25860438
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18344577.0936810892375455No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT407450.15755726952497864No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT400030.15468802191208053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2384350.086.45318
GTGGTAT2378150.085.901596
AGTGGTA2387950.085.613945
AAGCAGT2325550.085.58641
TGGTATC2406750.085.542937
GCAGTGG2392300.085.4018553
GTATCAA2411300.085.348489
AGCAGTG2453800.082.949712
CAGTGGT2477850.082.347334
CAACGCA2345600.045.05908612-13
ATCAACG2360900.044.83973710-11
ACGCAGA2370400.044.6966214-15
AGAGTAC2400750.043.98709518-19
TCAACGC2344600.043.8770712-13
AACGCAG2363450.043.61455514-15
GAGTACT2349600.043.4860920-21
CGCAGAG2383300.043.2907316-17
AGTACTT2449600.042.8175420-21
TATCAAC2404750.042.77184710-11
GTACTTT2443100.041.78642322-23
Produced by FastQC (version 0.11.5)