##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660706_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 25860438 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.276644308963366 34.0 31.0 34.0 27.0 34.0 2 31.912018388861007 34.0 31.0 34.0 30.0 34.0 3 31.659950732466324 34.0 31.0 34.0 30.0 34.0 4 35.58163469621048 37.0 35.0 37.0 33.0 37.0 5 35.915533062510384 37.0 35.0 37.0 35.0 37.0 6 35.38199797698709 37.0 35.0 37.0 33.0 37.0 7 35.57375810881471 37.0 35.0 37.0 35.0 37.0 8 35.95583470009286 37.0 35.0 37.0 35.0 37.0 9 37.76990149973484 39.0 38.0 39.0 35.0 39.0 10-11 37.75032638658324 39.0 37.5 39.0 35.0 39.0 12-13 37.8663774372267 39.0 38.0 39.0 35.0 39.0 14-15 39.33051451023374 41.0 39.0 41.0 36.0 41.0 16-17 39.2962032390944 41.0 39.0 41.0 36.0 41.0 18-19 39.28521595419227 41.0 39.0 41.0 36.0 41.0 20-21 39.20692959647474 40.5 39.0 41.0 36.0 41.0 22-23 39.04764159446951 40.0 38.5 41.0 35.5 41.0 24-25 39.0845785751966 40.0 39.0 41.0 36.0 41.0 26-27 39.07426223794044 40.0 39.0 41.0 36.0 41.0 28-29 39.013265127218645 40.0 39.0 41.0 35.0 41.0 30-31 38.92737331053712 40.0 38.5 41.0 35.0 41.0 32-33 38.81423330880939 40.0 38.0 41.0 35.0 41.0 34-35 38.73533203497946 40.0 38.0 41.0 35.0 41.0 36-37 38.59340487581842 40.0 38.0 41.0 35.0 41.0 38-39 38.104469672942116 40.0 37.0 41.0 34.0 41.0 40-41 37.976227587483244 40.0 36.5 41.0 33.5 41.0 42-43 37.812922290798014 40.0 36.0 41.0 33.0 41.0 44-45 37.69508215599442 40.0 36.0 41.0 33.0 41.0 46-47 37.5537140940923 40.0 35.0 41.0 33.0 41.0 48-49 37.602693774946886 40.0 35.5 41.0 33.0 41.0 50-51 37.59817211912652 40.0 35.5 41.0 33.0 41.0 52-53 37.31165929981542 40.0 35.0 41.0 32.5 41.0 54-55 36.67137476557822 39.5 35.0 41.0 30.5 41.0 56-57 36.03673236315642 39.0 35.0 41.0 27.5 41.0 58-59 35.8665022997677 39.0 35.0 41.0 27.0 41.0 60-61 35.74037317156036 39.0 35.0 41.0 27.0 41.0 62-63 35.52912684618876 39.0 35.0 41.0 26.0 41.0 64-65 35.28942294016829 38.0 35.0 40.5 26.0 41.0 66-67 35.04346968137199 37.0 35.0 40.0 26.0 41.0 68-69 34.818774241178744 37.0 34.5 40.0 26.0 41.0 70-71 34.56168379669362 36.5 34.0 39.0 26.0 41.0 72-73 34.24995085156717 36.0 34.0 39.0 26.0 41.0 74-75 33.88560653922412 35.5 34.0 39.0 26.0 40.5 76-77 32.1798772897814 34.5 31.5 36.5 23.0 39.0 78-79 33.12613796409791 35.0 33.5 37.0 25.0 39.0 80-81 33.023757447573004 35.0 34.0 37.0 25.0 39.0 82-83 32.75785462334397 35.0 34.0 36.0 24.5 38.5 84-85 32.50378514470637 35.0 34.0 36.0 24.0 37.0 86-87 32.291333425984504 35.0 33.0 35.5 24.0 37.0 88-89 32.11786824724314 35.0 33.0 35.0 23.0 36.5 90-91 31.95826031639526 35.0 33.0 35.0 23.0 36.0 92-93 31.814524641848678 35.0 33.0 35.0 22.5 36.0 94-95 31.71014808411211 35.0 33.0 35.0 20.5 36.0 96-97 31.60988897403826 35.0 33.0 35.0 20.0 36.0 98-99 31.519439423261122 35.0 33.0 35.0 20.0 35.0 100 31.46577559900571 35.0 33.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.577355827676481 1101 2 1.1921101229762847 1101 3 1.5630882963625652 1101 4 0.9803135477565306 1101 5 0.5975819252432188 1101 6 1.1362053498838023 1101 7 0.8208553259549092 1101 8 0.49765989215236317 1101 9 0.5114483146583311 1101 10-11 0.43482324685217577 1101 12-13 0.3887114357560435 1101 14-15 0.5064823422003073 1101 16-17 0.5536501676961265 1101 18-19 0.5077036278909262 1101 20-21 0.523985779759137 1101 22-23 0.5378073685683802 1101 24-25 0.5281492899986233 1101 26-27 0.512529833548669 1101 28-29 0.4962810448704076 1101 30-31 0.4903250627176021 1101 32-33 0.520564016870253 1101 34-35 0.526953960693497 1101 36-37 0.488666253053502 1101 38-39 0.5682212068761885 1101 40-41 0.5406157173782447 1101 42-43 0.5615286502601933 1101 44-45 0.5477186574341175 1101 46-47 0.5635828542733137 1101 48-49 0.5381491240773499 1101 50-51 0.4934379734055767 1101 52-53 0.48948917063655273 1101 54-55 0.4800098862675739 1101 56-57 0.586898554069478 1101 58-59 0.5633154351680574 1101 60-61 0.5623535056510747 1101 62-63 0.5181539645764133 1101 64-65 0.5205642386302216 1101 66-67 0.5559669578329505 1101 68-69 0.5151342407157173 1101 70-71 0.5062354513596858 1101 72-73 0.475377564743809 1101 74-75 0.44017308832037116 1101 76-77 0.3605177965615738 1101 78-79 0.46949726637848954 1101 80-81 0.4637169966803114 1101 82-83 0.4327778726084759 1101 84-85 0.4614548655694506 1101 86-87 0.4357299940847099 1101 88-89 0.419736890416452 1101 90-91 0.42045823729147 1101 92-93 0.4314234319709307 1101 94-95 0.41153523609926523 1101 96-97 0.42179203682684374 1101 98-99 0.43967819726510626 1101 100 0.47708013532585625 1102 1 1.4699526054791257 1102 2 1.1325958445370752 1102 3 1.4917869078701855 1102 4 0.895582228167946 1102 5 0.49008190595324663 1102 6 1.0554012087120483 1102 7 0.764760041196304 1102 8 0.43628574187574287 1102 9 0.45879320397246204 1102 10-11 0.36035087588281556 1102 12-13 0.3432317519572976 1102 14-15 0.449013003614958 1102 16-17 0.4512669395574491 1102 18-19 0.40935378394682687 1102 20-21 0.42550619144576984 1102 22-23 0.40588993062422674 1102 24-25 0.4062485619949783 1102 26-27 0.43343820809824507 1102 28-29 0.42680543318531505 1102 30-31 0.43729815706100794 1102 32-33 0.4748170560057545 1102 34-35 0.4111143500809291 1102 36-37 0.4103859976928206 1102 38-39 0.442124258357282 1102 40-41 0.4648367882033142 1102 42-43 0.4655065854227374 1102 44-45 0.45059322533868595 1102 46-47 0.4621938169587949 1102 48-49 0.4493027417191797 1102 50-51 0.4000108345331981 1102 52-53 0.3834328468041761 1102 54-55 0.4298858613757872 1102 56-57 0.5072646102727489 1102 58-59 0.4416200730566615 1102 60-61 0.4323194540233928 1102 62-63 0.3952282637716351 1102 64-65 0.37553855885150966 1102 66-67 0.36162173673125864 1102 68-69 0.35675725577733175 1102 70-71 0.3167715342217008 1102 72-73 0.31461909258695897 1102 74-75 0.31135300302010194 1102 76-77 0.18519956985036146 1102 78-79 0.35636349426253133 1102 80-81 0.3644099022134526 1102 82-83 0.3451115158673943 1102 84-85 0.3376885028484864 1102 86-87 0.3529476901287225 1102 88-89 0.33598570890550405 1102 90-91 0.3433338950796099 1102 92-93 0.3168765004271883 1102 94-95 0.3217000641098515 1102 96-97 0.2982888038271021 1102 98-99 0.358706787900978 1102 100 0.34651817978539157 1103 1 1.46041539354108 1103 2 1.0765841519826473 1103 3 1.4278453587008144 1103 4 0.8926140320462608 1103 5 0.4923063138349377 1103 6 1.0148778264981075 1103 7 0.7389022928887456 1103 8 0.42828946147734825 1103 9 0.41936330646686315 1103 10-11 0.3509089445848943 1103 12-13 0.3033986690339603 1103 14-15 0.3805588890748197 1103 16-17 0.40879104513783204 1103 18-19 0.3564816745176529 1103 20-21 0.3650540485224596 1103 22-23 0.3760536316332477 1103 24-25 0.37581907086282484 1103 26-27 0.367490494934934 1103 28-29 0.33419062635563535 1103 30-31 0.36327447377609445 1103 32-33 0.39509364415513204 1103 34-35 0.38644696565672376 1103 36-37 0.34715184137184707 1103 38-39 0.36453699753138835 1103 40-41 0.40288068811458544 1103 42-43 0.3979198818676366 1103 44-45 0.33994835356886455 1103 46-47 0.3632968250269144 1103 48-49 0.3295515595089995 1103 50-51 0.33253090261431595 1103 52-53 0.3621662743307539 1103 54-55 0.35311419801656285 1103 56-57 0.2862382712668321 1103 58-59 0.280435173321969 1103 60-61 0.33736013388652 1103 62-63 0.2650085271942615 1103 64-65 0.24698364071965528 1103 66-67 0.2731541228489931 1103 68-69 0.26575449674411544 1103 70-71 0.22530042527453276 1103 72-73 0.2144647014378691 1103 74-75 0.25213544830262435 1103 76-77 0.10139627992624867 1103 78-79 0.25677174939702496 1103 80-81 0.2830546142202479 1103 82-83 0.2677902405712871 1103 84-85 0.21293283080886027 1103 86-87 0.23140273013641632 1103 88-89 0.24159477783628347 1103 90-91 0.25706187112275103 1103 92-93 0.2388205802934955 1103 94-95 0.22391255128176724 1103 96-97 0.26430736818174694 1103 98-99 0.2552541940949382 1103 100 0.25283867273121174 1104 1 1.3928207704561224 1104 2 1.0389972041357716 1104 3 1.3919798212919474 1104 4 0.8580533574042377 1104 5 0.4716526187036294 1104 6 1.0055487337342015 1104 7 0.7435112503602568 1104 8 0.38640807802963906 1104 9 0.3885167985694409 1104 10-11 0.30564741237395054 1104 12-13 0.26710245600310856 1104 14-15 0.30028328036433294 1104 16-17 0.33527128868678346 1104 18-19 0.29251373562939875 1104 20-21 0.31972507591920163 1104 22-23 0.3343207262576158 1104 24-25 0.3188618180120173 1104 26-27 0.30781300013608615 1104 28-29 0.28617408990068327 1104 30-31 0.29666850730789207 1104 32-33 0.33371229695404736 1104 34-35 0.32475681082960506 1104 36-37 0.32306453891109044 1104 38-39 0.3463202547083739 1104 40-41 0.3227254861531037 1104 42-43 0.34479960972254275 1104 44-45 0.31781949887439254 1104 46-47 0.3319662598490467 1104 48-49 0.2816666660230638 1104 50-51 0.2773710988785467 1104 52-53 0.294739548520802 1104 54-55 0.2859875431140111 1104 56-57 0.2984307620356361 1104 58-59 0.28508416896002586 1104 60-61 0.2673246285080353 1104 62-63 0.18796755012272115 1104 64-65 0.15508908066981775 1104 66-67 0.20292539447854097 1104 68-69 0.17066113636878555 1104 70-71 0.19339262909597466 1104 72-73 0.183885063378888 1104 74-75 0.20291320622282427 1104 76-77 0.11925047968919955 1104 78-79 0.25507093485028776 1104 80-81 0.28850524224650087 1104 82-83 0.29274713878514547 1104 84-85 0.2577648179415135 1104 86-87 0.277754275667327 1104 88-89 0.2312665920760537 1104 90-91 0.23923616567540762 1104 92-93 0.2380454118567208 1104 94-95 0.25214764167535364 1104 96-97 0.21674788670168965 1104 98-99 0.2507577449481069 1104 100 0.28729959804169525 1105 1 1.3719114520747588 1105 2 1.0041707943774831 1105 3 1.3554740837449586 1105 4 0.8403770228379912 1105 5 0.4467207008664005 1105 6 0.9865786821708298 1105 7 0.715576205083309 1105 8 0.3678510541764197 1105 9 0.36042913067183946 1105 10-11 0.27102197132195016 1105 12-13 0.23854773692240627 1105 14-15 0.3072111797724517 1105 16-17 0.3123238438943261 1105 18-19 0.3077679545276908 1105 20-21 0.31994070858881685 1105 22-23 0.3190554356074031 1105 24-25 0.31734577497897476 1105 26-27 0.3161814787224131 1105 28-29 0.2963495459716725 1105 30-31 0.30043480832582503 1105 32-33 0.3648052903538428 1105 34-35 0.33248279962699456 1105 36-37 0.3360924293548919 1105 38-39 0.35804745015336437 1105 40-41 0.37523607770578593 1105 42-43 0.35053555694273797 1105 44-45 0.34133252707204065 1105 46-47 0.3634605593030571 1105 48-49 0.34680034812650007 1105 50-51 0.35972479423205783 1105 52-53 0.35836864235107413 1105 54-55 0.4218316046733932 1105 56-57 0.44680910548760977 1105 58-59 0.3944439959740933 1105 60-61 0.3622830405130131 1105 62-63 0.3381427257835057 1105 64-65 0.28845060080801943 1105 66-67 0.29275374558720557 1105 68-69 0.2671961155325988 1105 70-71 0.2688780178530763 1105 72-73 0.250962333436334 1105 74-75 0.27843811333706725 1105 76-77 0.18498029149294837 1105 78-79 0.2934439667802664 1105 80-81 0.31957616471237316 1105 82-83 0.32348196411458474 1105 84-85 0.2937044898964487 1105 86-87 0.31318620003020214 1105 88-89 0.30098846187854633 1105 90-91 0.3013150763534469 1105 92-93 0.2926835142000819 1105 94-95 0.23754514829875362 1105 96-97 0.2505042633627532 1105 98-99 0.297168068893054 1105 100 0.2870162382778325 1106 1 1.3098607114957623 1106 2 0.9549099718611096 1106 3 1.303956981785639 1106 4 0.7749999021661225 1106 5 0.3797551569655795 1106 6 0.9073671562374201 1106 7 0.6356795480708399 1106 8 0.31505323675569485 1106 9 0.28008228810131897 1106 10-11 0.18404463876631638 1106 12-13 0.17649679021588582 1106 14-15 0.18945108441495506 1106 16-17 0.18343012559356708 1106 18-19 0.18900285650137505 1106 20-21 0.19868842922270602 1106 22-23 0.23816652901248858 1106 24-25 0.20150363867892196 1106 26-27 0.1794924622393168 1106 28-29 0.1764130658895695 1106 30-31 0.194440137434718 1106 32-33 0.22647257781681418 1106 34-35 0.24593027442433169 1106 36-37 0.20887708097445312 1106 38-39 0.2588064561077914 1106 40-41 0.26278560108648463 1106 42-43 0.27106654249440254 1106 44-45 0.2664683070592915 1106 46-47 0.2584647432701601 1106 48-49 0.23640542949311794 1106 50-51 0.24094210333726807 1106 52-53 0.24478062383702337 1106 54-55 0.26970980351092777 1106 56-57 0.2634114255041382 1106 58-59 0.22835483883540775 1106 60-61 0.17640375382792683 1106 62-63 0.19053262298204743 1106 64-65 0.1499770924503565 1106 66-67 0.12427999117919342 1106 68-69 0.1123132769179449 1106 70-71 0.10714618565831557 1106 72-73 0.09600603296761534 1106 74-75 0.13308117277880882 1106 76-77 0.019467970328008732 1106 78-79 0.16156526076175481 1106 80-81 0.17046805893021144 1106 82-83 0.20142199745190936 1106 84-85 0.20111941308257286 1106 86-87 0.17817622012518797 1106 88-89 0.18736561547717656 1106 90-91 0.22452050741041063 1106 92-93 0.19939869424176848 1106 94-95 0.1929074985165613 1106 96-97 0.21444741851279758 1106 98-99 0.23822368988962594 1106 100 0.21390772355094256 1107 1 1.276659195693771 1107 2 0.9059320439540635 1107 3 1.2725857273342385 1107 4 0.78015404405091 1107 5 0.383877129696387 1107 6 0.9047585479891467 1107 7 0.6415826232471602 1107 8 0.2909325062797663 1107 9 0.25430098207254304 1107 10-11 0.17965251026593165 1107 12-13 0.13130556488486178 1107 14-15 0.15579486917525287 1107 16-17 0.15495627701083237 1107 18-19 0.10889735226649577 1107 20-21 0.12280070576817081 1107 22-23 0.15763703651929717 1107 24-25 0.1602160580698353 1107 26-27 0.0944405270894535 1107 28-29 0.12614288494210513 1107 30-31 0.11699098747254055 1107 32-33 0.1718847751993664 1107 34-35 0.1942941086469787 1107 36-37 0.17216725919671916 1107 38-39 0.170009213011582 1107 40-41 0.17214208204626402 1107 42-43 0.18151992458145827 1107 44-45 0.17749837287478698 1107 46-47 0.21368133469837147 1107 48-49 0.18047911513042436 1107 50-51 0.15388313766121797 1107 52-53 0.14079603331467894 1107 54-55 0.16461196448195636 1107 56-57 0.16397493864528911 1107 58-59 0.12102825794766403 1107 60-61 0.11590982622620061 1107 62-63 0.08315330647020858 1107 64-65 0.07189431587482176 1107 66-67 0.07978646543107004 1107 68-69 0.04711757663777405 1107 70-71 0.03491213725046549 1107 72-73 0.04418564025261418 1107 74-75 0.05882072949815864 1107 76-77 -0.04968680250723878 1107 78-79 0.08599474506739568 1107 80-81 0.14366801852007427 1107 82-83 0.1609657898225052 1107 84-85 0.1984617072318997 1107 86-87 0.1581828599344206 1107 88-89 0.15774846986111868 1107 90-91 0.1494221551175059 1107 92-93 0.14819172307115736 1107 94-95 0.09745352080835801 1107 96-97 0.13246723184309417 1107 98-99 0.17018350254311088 1107 100 0.1646584860704401 1108 1 1.2374478211423074 1108 2 0.877521757114426 1108 3 1.2396534045681094 1108 4 0.7547077922628347 1108 5 0.33687434441677766 1108 6 0.8892966868157757 1108 7 0.5914497115973774 1108 8 0.2508859451783323 1108 9 0.21756153546989054 1108 10-11 0.13302098634135007 1108 12-13 0.11997207033778778 1108 14-15 0.12819884781562507 1108 16-17 0.15663297939562426 1108 18-19 0.09427150160242093 1108 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-0.9390408636318455 2316 80-81 -1.1557899567181096 2316 82-83 -1.221997543542237 2316 84-85 -0.8821049690728628 2316 86-87 -0.680659284139935 2316 88-89 -0.6230888167482647 2316 90-91 -0.6839856069861412 2316 92-93 -0.7376636372797485 2316 94-95 -0.7917230399417718 2316 96-97 -0.8797276043577398 2316 98-99 -1.064878688513872 2316 100 -0.9404035976195253 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 63.0 7 754.0 8 951.0 9 364.0 10 347.0 11 608.0 12 903.0 13 1178.0 14 1850.0 15 3469.0 16 6389.0 17 11285.0 18 17613.0 19 28847.0 20 44677.0 21 69113.0 22 110670.0 23 193718.0 24 364388.0 25 540715.0 26 554404.0 27 434944.0 28 345068.0 29 334736.0 30 376223.0 31 455108.0 32 564130.0 33 723645.0 34 1001699.0 35 1579575.0 36 2824512.0 37 5560096.0 38 7357047.0 39 2345897.0 40 5452.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 16.384308778298575 34.85130847086857 27.644305491389087 21.12007725944377 2 20.691745439775712 34.86950902249338 26.77324717916686 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25.842901728203298 19.805875050939463 22-23 24.005696656906 26.04923185636836 30.103228559942185 19.84184292678345 24-25 19.84490328134054 30.083728678478977 25.772356214531804 24.29901182564868 26-27 19.891008880359735 25.832404662449886 34.378019437160866 19.898567020029514 28-29 19.88905607442626 25.914551273764175 34.36064332224789 19.835749329561676 30-31 19.880835544903896 25.85818325387612 34.34748763630977 19.913493564910212 32-33 20.015014511539714 25.949944769787226 34.16171304198405 19.873327676689016 34-35 19.992108756457196 26.055911616525396 34.101806903339515 19.85017272367789 36-37 19.942097663256128 26.01616416064621 34.13244139578159 19.909296780316065 38-39 19.940387868083675 26.01032530340116 34.15441309829561 19.894873730219555 40-41 19.951753885259357 26.052660257570427 34.128433604627816 19.8671522525424 42-43 19.959726361728002 26.05377462237107 34.08465230314107 19.901846712759856 44-45 19.966890019327565 26.111148364599472 34.0574644266633 19.864497189409658 46-47 19.986105714128055 26.18304463495335 33.98196930255996 19.84888034835863 48-49 20.00004642178017 26.193597778872558 33.90081445079069 19.905541348556582 50-51 20.025172707185373 26.237331537524707 33.82586541601717 19.911630339272744 52-53 20.130675708565153 26.372781749666174 33.549660986846916 19.946881554921756 54-55 20.508022426727514 26.67273436353434 32.63999665166492 20.179246558073228 56-57 21.064484581083246 27.37569853720917 31.096949336412493 20.462867545295087 58-59 21.147669874979787 28.07410247632866 30.21803902907245 20.560188619619097 60-61 21.083893692334385 28.463895414195363 29.84150408965187 20.610706803818385 62-63 21.015808506931712 28.68668931080801 29.659288136023278 20.638214046236996 64-65 20.97255626147838 28.763941958618094 29.649669119308314 20.613832660595207 66-67 20.890978848588205 28.514551654446468 29.96707472013222 20.627394776833107 68-69 20.793480322738635 28.25288332350827 30.348126326716862 20.60551002703623 70-71 20.745028613575062 28.082650768434707 30.64572102645544 20.52659959153479 72-73 20.692070809679862 27.993819381861567 30.74854234701662 20.56556746144195 74-75 20.75322115086981 28.080877004271652 30.6007223890299 20.565179455828638 76-77 20.808778038722 28.263650082406272 30.371299018509184 20.55627286036254 78-79 20.929511794913857 28.469737072719468 29.962605880684706 20.638145251681962 80-81 21.031244085388 28.73128932080484 29.564291087663392 20.673175506143767 82-83 21.072392868265638 28.95278137253195 29.19837820166861 20.776447557533796 84-85 21.083932214101996 29.06896334800948 28.97278726332903 20.874317174559497 86-87 21.120802787541002 29.136886814465285 28.816031576066443 20.92627882192727 88-89 21.159519919095 29.267691506662143 28.62923471628072 20.943553857962137 90-91 21.23000913711588 29.268599111183374 28.459839861640667 21.041551890060077 92-93 21.246977563000264 29.358235361127626 28.33064741057131 21.0641396653008 94-95 21.263861385448948 29.435672367070087 28.209737221303705 21.090729026177254 96-97 21.28233036966734 29.423107709833445 28.122250187111593 21.172311733387623 98-99 21.31833410456904 29.444384543268104 28.05503833565671 21.182243016506145 100 21.289334531227862 29.47704571737238 27.987752209082096 21.24586754231767 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 31702.0 1 24112.0 2 13071.0 3 8825.0 4 7713.0 5 7365.5 6 7392.5 7 7348.0 8 7191.0 9 7313.5 10 8215.0 11 18686.5 12 158177.0 13 185818.5 14 81637.5 15 85859.5 16 98713.5 17 111132.0 18 121361.0 19 130405.5 20 142086.5 21 157947.5 22 178544.5 23 204533.5 24 233885.0 25 266340.0 26 305549.5 27 352264.0 28 404432.5 29 453874.0 30 493582.0 31 528648.5 32 567873.5 33 604335.0 34 632990.0 35 664995.0 36 703695.5 37 738535.0 38 763748.0 39 777996.5 40 781040.0 41 786399.5 42 801233.5 43 806497.5 44 806972.0 45 812409.0 46 809932.5 47 803577.0 48 790227.5 49 767482.5 50 751588.0 51 739230.0 52 718436.5 53 684409.5 54 640890.5 55 599697.0 56 559761.5 57 514299.5 58 460876.5 59 401736.0 60 336397.5 61 273662.0 62 218261.5 63 167357.5 64 124941.5 65 92207.0 66 68737.5 67 53001.5 68 41259.5 69 32655.5 70 26610.5 71 22107.0 72 18796.0 73 15592.0 74 12870.0 75 10712.0 76 8388.0 77 5983.0 78 4146.0 79 2884.0 80 1895.5 81 1254.5 82 795.5 83 443.0 84 265.0 85 165.5 86 100.0 87 53.0 88 38.0 89 34.5 90 24.0 91 17.0 92 13.0 93 9.5 94 7.0 95 5.5 96 3.5 97 2.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3304893753152982 2 0.0064848089579921265 3 2.4174455204509684 4 9.512599902600259E-4 5 4.640292635414759E-5 6 0.008835890559935606 7 0.007958101869736313 8 3.7122341083318074E-4 9 3.8669105295123E-6 10-11 1.0634003956158825E-4 12-13 1.93345526475615E-6 14-15 0.0 16-17 3.673565003036685E-5 18-19 0.0 20-21 3.2288702921427705E-4 22-23 2.900182897134225E-5 24-25 4.7562999513001293E-4 26-27 0.00348021947656107 28-29 0.006221859041985291 30-31 0.006003378597067845 32-33 0.015927804471061163 34-35 0.010297582740091255 36-37 0.005328602709667949 38-39 0.005568351162497712 40-41 0.008468534059631936 42-43 0.01958010146618553 44-45 0.034873732610406674 46-47 0.03404234684656153 48-49 0.040614161291467686 50-51 0.04105112218130257 52-53 0.06306157691528658 54-55 0.033966942091236046 56-57 0.0223681439579639 58-59 0.01693320120873436 60-61 0.01814354420447171 62-63 0.02517358754712507 64-65 0.04580742213260271 66-67 0.048405986008434966 68-69 0.051310035816098706 70-71 0.0614336075823619 72-73 0.06975519904187237 74-75 0.06047074686051335 76-77 0.060406942836776396 78-79 0.05463171196094977 80-81 0.05194807605346823 82-83 0.048676669745500833 84-85 0.050486383873312585 86-87 0.05913859618309635 88-89 0.06494089543262956 90-91 0.06128859843750519 92-93 0.06594629217030276 94-95 0.07434522184040349 96-97 0.07311167738148905 98-99 0.047597801707766896 100 0.006705222858174328 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.5860438E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.890760999786636 #Duplication Level Percentage of deduplicated Percentage of total 1 62.62327469602379 23.72843534531023 2 18.606448408960762 14.100249794375848 3 7.601207823242011 8.640466468205142 4 3.491890089858871 5.292414913294638 5 1.8661121556571139 3.5354204844400163 6 1.2184858365979037 2.7701613369693736 7 0.8277277187746632 2.195426321549255 8 0.5731144075530892 1.7372592833702727 9 0.44186161122059303 1.5068225435166116 >10 2.4799756073391186 17.725815379926253 >50 0.17652717124059866 4.5580906922092765 >100 0.09215836607663258 5.84489136808387 >500 4.3946839129019744E-4 0.10531206981205163 >1k 6.745647180774507E-4 0.5513336303567271 >5k 4.0829738180541167E-5 0.11843980012426666 >10k+ 6.124460727080826E-5 7.589460568456207 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1834457 7.0936810892375455 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 40745 0.15755726952497864 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 40003 0.15468802191208053 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0015390303907458953 7.7338210590246E-6 0.0 0.0 0.0 2 0.0015428973012754077 7.7338210590246E-6 0.0 3.8669105295123E-6 3.8669105295123E-6 3 0.0015506311223344323 7.7338210590246E-6 0.0 3.8669105295123E-6 7.7338210590246E-6 4 0.0015506311223344323 1.54676421180492E-5 0.0 3.8669105295123E-6 7.7338210590246E-6 5 0.0015506311223344323 4.25360158246353E-5 0.0 3.8669105295123E-6 1.54676421180492E-5 6 0.0015544980328639445 4.64029263541476E-5 0.0 3.8669105295123E-6 1.54676421180492E-5 7 0.0015544980328639445 5.0269836883659895E-5 0.0 3.8669105295123E-6 1.54676421180492E-5 8 0.001562231853922969 5.0269836883659895E-5 0.0 3.8669105295123E-6 1.54676421180492E-5 9 0.001562231853922969 5.80036579426845E-5 0.0 3.8669105295123E-6 1.54676421180492E-5 10-11 0.0015680322197172375 6.57374790017091E-5 0.0 3.8669105295123E-6 1.54676421180492E-5 12-13 0.0015699656749819937 7.34713000607337E-5 0.0 3.8669105295123E-6 2.126800791231765E-5 14-15 0.0015699656749819937 7.540475532548984E-5 0.0 3.8669105295123E-6 2.32014631770738E-5 16-17 0.0015796329513057745 8.50720316492706E-5 0.0 3.8669105295123E-6 2.32014631770738E-5 18-19 0.0015951005934238237 9.087239744353905E-5 3.8669105295123E-6 1.16007315885369E-5 2.32014631770738E-5 20-21 0.0016105682355418728 1.1794077115012515E-4 3.8669105295123E-6 1.16007315885369E-5 2.70683737065861E-5 22-23 0.0016279693329246782 1.2954150273866206E-4 3.8669105295123E-6 1.16007315885369E-5 2.70683737065861E-5 24-25 0.0017072409987796805 1.353418685329305E-4 3.8669105295123E-6 1.16007315885369E-5 2.70683737065861E-5 26-27 0.0019392556305504183 1.4114223432719895E-4 3.8669105295123E-6 1.16007315885369E-5 3.09352842360984E-5 28-29 0.002737772654894708 1.5660987644524813E-4 3.8669105295123E-6 1.16007315885369E-5 3.48021947656107E-5 30-31 0.004474015482645731 1.7594442909280963E-4 3.8669105295123E-6 1.16007315885369E-5 3.8669105295122995E-5 32-33 0.007936833861823995 1.93345526475615E-4 3.8669105295123E-6 1.353418685329305E-5 4.64029263541476E-5 34-35 0.013870608069360617 1.9914589226988343E-4 3.8669105295123E-6 1.54676421180492E-5 4.64029263541476E-5 36-37 0.02341801016672649 2.165469896526888E-4 3.8669105295123E-6 1.740109738280535E-5 4.64029263541476E-5 38-39 0.0374606957546504 2.165469896526888E-4 3.8669105295123E-6 1.9334552647561498E-5 4.64029263541476E-5 40-41 0.056016065930515176 2.281477212412257E-4 3.8669105295123E-6 2.32014631770738E-5 4.64029263541476E-5 42-43 0.0796834918263952 2.4168190809451874E-4 3.8669105295123E-6 3.2868739500854545E-5 4.833638161890375E-5 44-45 0.10918801916657406 2.5908300547732407E-4 7.7338210590246E-6 3.8669105295122995E-5 5.0269836883659895E-5 46-47 0.14678212333449264 2.822844686543979E-4 7.7338210590246E-6 4.060256055987914E-5 5.41367474131722E-5 48-49 0.19406090492357475 2.9195174497817863E-4 7.7338210590246E-6 4.25360158246353E-5 5.41367474131722E-5 50-51 0.24836586294478075 3.074193870962278E-4 1.16007315885369E-5 4.25360158246353E-5 5.80036579426845E-5 52-53 0.31050131478824916 3.132197528904963E-4 1.16007315885369E-5 4.25360158246353E-5 6.18705684721968E-5 54-55 0.3808906871569615 3.3062085027330164E-4 1.16007315885369E-5 4.25360158246353E-5 6.18705684721968E-5 56-57 0.46397512679406283 3.441550371265947E-4 1.16007315885369E-5 4.64029263541476E-5 6.380402373695296E-5 58-59 0.5597991031706424 3.5188885818561926E-4 1.16007315885369E-5 5.0269836883659895E-5 6.767093426646525E-5 60-61 0.6681712042154893 3.5575576871513156E-4 1.16007315885369E-5 5.607020267792835E-5 7.34713000607337E-5 62-63 0.7884746576991465 3.596226792446439E-4 1.16007315885369E-5 5.993711320744065E-5 7.34713000607337E-5 64-65 0.9194875198942879 3.712234108331808E-4 1.54676421180492E-5 6.18705684721968E-5 7.540475532548984E-5 66-67 1.064214380282345 3.7702377662744924E-4 1.9334552647561498E-5 8.12051211197583E-5 7.733821059024599E-5 68-69 1.2251126605048221 3.8669105295123E-4 1.9334552647561498E-5 8.12051211197583E-5 7.733821059024599E-5 70-71 1.4009855517528358 3.9055796348074227E-4 1.9334552647561498E-5 8.12051211197583E-5 7.733821059024599E-5 72-73 1.5906246444859131 4.0022523980452307E-4 1.9334552647561498E-5 8.12051211197583E-5 8.12051211197583E-5 74-75 1.7938327262670493 4.1569288192257225E-4 2.32014631770738E-5 8.12051211197583E-5 8.12051211197583E-5 76-77 2.0124523799635567 4.1955979245208454E-4 2.32014631770738E-5 8.313857638451445E-5 8.12051211197583E-5 78-79 2.2488926908353215 4.214932477168407E-4 2.32014631770738E-5 8.50720316492706E-5 8.12051211197583E-5 80-81 2.5010461926437593 4.214932477168407E-4 2.32014631770738E-5 8.893894217878289E-5 9.28058527082952E-5 82-83 2.773765858103409 4.330939793053776E-4 2.32014631770738E-5 8.893894217878289E-5 1.0053967376731979E-4 84-85 3.0639504249696 4.524285319529391E-4 2.32014631770738E-5 8.893894217878289E-5 1.044065842968321E-4 86-87 3.3710005221102595 4.6209580827671985E-4 2.32014631770738E-5 9.087239744353905E-5 1.082734948263444E-4 88 3.613399742108003 4.678961740709883E-4 2.70683737065861E-5 9.28058527082952E-5 1.082734948263444E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 238435 0.0 86.4531 8 GTGGTAT 237815 0.0 85.90159 6 AGTGGTA 238795 0.0 85.61394 5 AAGCAGT 232555 0.0 85.5864 1 TGGTATC 240675 0.0 85.54293 7 GCAGTGG 239230 0.0 85.401855 3 GTATCAA 241130 0.0 85.34848 9 AGCAGTG 245380 0.0 82.94971 2 CAGTGGT 247785 0.0 82.34733 4 CAACGCA 234560 0.0 45.059086 12-13 ATCAACG 236090 0.0 44.839737 10-11 ACGCAGA 237040 0.0 44.69662 14-15 AGAGTAC 240075 0.0 43.987095 18-19 TCAACGC 234460 0.0 43.87707 12-13 AACGCAG 236345 0.0 43.614555 14-15 GAGTACT 234960 0.0 43.48609 20-21 CGCAGAG 238330 0.0 43.29073 16-17 AGTACTT 244960 0.0 42.81754 20-21 TATCAAC 240475 0.0 42.771847 10-11 GTACTTT 244310 0.0 41.786423 22-23 >>END_MODULE